Background and Objective: Dental implantology, a pivotal aspect of restorative dentistry, has seen significant advancements, including the widespread use of implant-supported crowns. The choice of luting agent in these restorations plays a crucial role in determining their long-term success. Therefore, this study aimed to comprehensively investigate and compare the effectiveness of different luting agents. Methodology: In this prospective, randomized controlled trial, sixty participants requiring single-tooth implant-supported crowns were randomly assigned to three groups: resin-based, glass ionomer, and zinc oxide-eugenol. Rigorous inclusion and exclusion criteria were applied, and the study adhered to standardized protocols for crown placement and luting agent application. Clinical assessments, radiographic analysis, and patient-reported outcomes were systematically collected at baseline, post-implant placement, and during follow-up visits. Results: Findings revealed no statistically significant differences in retention values among the luting agent groups (Resin-Based: 5.40 mm, Glass Ionomer: 5.15 mm, Zinc Oxide-Eugenol: 5.30 mm; P > 0.05). Similarly, no significant variations were observed in clinical performance indicators, osseointegration scores (Resin-Based: 9.5, Glass Ionomer: 9.0, Zinc Oxide-Eugenol: 9.3; P > 0.05), or patient-reported outcomes (Discomfort: Control 2.1, Resin-Based 2.3, Glass Ionomer 2.0, Zinc Oxide-Eugenol 2.2; Satisfaction: Control 4.6, Resin-Based 4.4, Glass Ionomer 4.8, Zinc Oxide-Eugenol 4.5; P > 0.05). Conclusion: The study provides compelling evidence that the choice of luting agent does not significantly influence the retention and overall clinical performance of implant-supported crowns. Practitioners can, therefore, exercise flexibility in selecting luting agents based on clinical preferences without compromising key outcomes.