Although tissue and cell manipulation nowadays is a common task in biomedical analysis, there are still many different ways to accomplish it, most of which are still not sufficiently general, inexpensive, accurate, efficient or effective. Several problems arise both for in vivo or in vitro analysis, such as the maximum overall size of the device and the gripper jaws (like in minimally-invasive open biopsy) or very limited manipulating capability, degrees of freedom or dexterity (like in tissues or cell-handling operations). This paper presents a new approach to tissue and cell manipulation, which employs a conceptually new conjugate surfaces flexure hinge (CSFH) silicon MEMS-based technology micro-gripper that solves most of the above-mentioned problems. The article describes all of the phases of the development, including topology conception, structural design, simulation, construction, actuation testing and in vitro observation. The latter phase deals with the assessment of the function capability, which consists of taking a series of in vitro images by optical microscopy. They offer a direct morphological comparison between the gripper and a variety of tissues.

Pollution derived from transportation systems is a worldwide, timelier issue than ever. The abatement actions of harmful substances in the air are on the agenda and they are necessary today to safeguard our welfare and that of the planet. Environmental pollution in large cities is approximately 20% due to the transportation system. In addition, private traffic contributes greatly to city pollution. Further, “vehicle operating life” is most often exceeded and vehicle emissions do not comply with European antipollution standards. It becomes mandatory to find a solution that respects the environment and, realize an appropriate transportation service to the customers. New technologies related to hybrid–electric engines are making great strides in reducing emissions, and the funds allocated by public authorities should be addressed. In addition, the use (implementation) of new technologies is also convenient from an economic point of view. In fact, by implementing the use of hybrid vehicles, fuel consumption can be reduced. The different hybrid configurations presented refer to such a series architecture, developed by the researchers and Research and Development groups. Regarding energy flows, different strategy logic or vehicle management units have been illustrated. Various configurations and vehicles were studied by simulating different driving cycles, both European approval and homologation and customer ones (typically municipal and university). The simulations have provided guidance on the optimal proposed configuration and information on the component to be used.

Nowadays, the Organic Rankine Cycle (ORC) system, which operates with organic fluids, is one of the leading technologies for “waste energy recovery”. It works as a conventional Rankine Cycle but, as mentioned, instead of steam/water, an organic fluid is used. This change allows it to convert low temperature heat into electric energy where required. Large numbers of studies have been carried out to identify the most suitable fluids, system parameters and the various configurations. In the present market, most ORC systems are designed and manufactured for the recovery of thermal energy from various sources operating at “large power rating” (exhaust gas turbines, internal combustion engines, geothermal sources, large melting furnaces, biomass, solar, etc.); from which it is possible to produce a large amount of electric energy (30 kW ÷ 300 kW). Such applications for small nominal power sources, as well as the exhaust gases of internal combustion engines (car sedan or town, ships, etc.) or small heat exchangers, are very limited. The few systems that have been designed and built for small scale applications, have, on the other hand, different types of expander (screw, scroll, etc.). These devices are not adapted for placement in small and restricted places like the interior of a conventional car. The aim of this work is to perform the preliminary design of a turbo-expander that meets diverse system requirements such as low pressure, small size and low mass flow rates. The expander must be adaptable to a small ORC system utilizing gas of a diesel engine or small gas turbine as thermal source to produce 2–10 kW of electricity. The temperature and pressure of the exhaust gases, in this case study (400–600 °C and a pressure of 2 bar), imposes a limit on the use of an organic fluid and on the net power that can be produced. In addition to water, fluids such as CO₂, R134a and R245fa have been considered. Once the operating fluids has been chosen, the turbine characteristics (dimensions, input and output temperature, pressure ratio, etc.) have been calculated and an attempt to find the “nearly-optimal” combination has been carried out. The detailed design of a radial expander is presented and discussed. A thermo-mechanical performance study was carry out to verify structural tension and possible displacement. On the other hand, preliminary CFD analyses have been performed to verify the effectiveness of the design procedure.