The transformation from natural fermentation to synthetic fermentation is essential in constructing a constant food fermentation process, which is the premise for stably making high-quality food. According to flavor-producing and cooccurring functions in dominant microbes, we provided a system-level approach to identify the core microbiota in Chinese light-aroma-type liquor fermentation. In addition, we successfully constructed a synthetic core microbiota to simulate the microbial community succession and flavor compound production in the in vitro system. The constructed synthetic core microbiota could not only facilitate a mechanistic understanding of the structure and function of the microbiota but also be beneficial for constructing a tractable and reproducible food fermentation process.

Lignocellulosic biomass, including agricultural, forestry, and energy crop waste, is one of Earth’s most abundant renewable resources, accounting for approximately 50% of global renewable resources. It contains cellulose, hemicellulose, and lignin, making it crucial for biofuels and bio-based chemicals. Due to its complex structure, single-pretreatment methods are inefficient, leading to the development of combined pretreatment technologies. These methods enhance cellulose accessibility and conversion efficiency. This paper analyzes the principles, advantages, and disadvantages of various combined pretreatment methods and their practical benefits. It highlights recent research achievements and applications in biofuel, biochemical production, and feed. By integrating multiple pretreatment methods, biomass degradation efficiency can be significantly improved, energy consumption reduced, and chemical reagent use minimized. Future advancements in combined physical, chemical, and biological pretreatment technologies will further enhance biomass utilization efficiency, reduce energy consumption, and protect the environment, providing robust support for sustainable renewable energy development and ecological protection.

We used Chinese liquor fermentation as a model system to show that microbiome composition changes more dramatically across seasons than throughout the fermentation process within seasons. These changes translate to differences in the metabolome as the ultimate functional outcome of microbial activity, suggesting that temporal changes in microbiome composition are translating into functional changes. This result is striking as it suggests that microbial functioning, despite controlled conditions in the fermentors, fluctuates over season along with external temperature differences, which threatens a reproducible food taste. As such, we believe that our study provides a stepping-stone into novel taxonomy-functional studies that promote future work in other systems and that also is relevant in applied settings to better control surrounding conditions in food production.

Low and unstable digestion performance is a challenging issue for anaerobic digestion, which prompts researchers to develop new strategies. In addition to traditional approaches such as co-digestion, pre-treatment, and recirculation, some emerging strategies, namely additive processes and microaeration, have also been recognized and developed in recent years. Many studies have evaluated the effect of these strategies on digestion performance. However, their comprehensive analysis is lacking, especially regarding the mechanisms of the different strategies. This review presents a comprehensive overview of research progress on these strategies based on the latest research, considering the five main strategies listed above. Through critical thinking, a summary of their mechanism, reactor performance, and availability of these strategies is presented. The results demonstrate that the contribution of microaeration is mainly to balance the composition and activity of hydrolysis, acidogenesis, and methanogenic archaea. Recirculation and co-digestion mainly balance mass and reaction environments. Pre-treatment, such as removing lignin, reducing cellulose crystallinity, and increasing the substrate-specific surface area, makes the characteristics of the substrate more conducive to the digestion of microorganisms. The mechanism of additive strategies varies greatly depending on the type of additive, such as enhancing interspecies electron transfer through conductive materials, resisting adverse digestion conditions through functional microbial additives, and accelerating nutrient absorption by regulating the bioavailability of trace elements. Although these strategies have different mechanisms for promoting digestion performance, their ultimate effect is to allow the parameters of the reactor to reach an ideal status and then achieve a balance among the substance, microorganisms, and water in an anaerobic reactor.