In Mexico, kiwifruit has adapted to high tropical climates, but plant numbers remain limited for its commercial cultivation. In vitro cultivation offers a solution for its propagation and commercial introduction. This study compared semisolid medium, partial immersion, and temporary bioreactor (BIT®) in vitro systems for plant multiplication and field introduction. For the BIT® system, five immersion times (2, 3, 5, 8, and 11 min) were tested for multiplication. After 60 days, the number of shoots, roots, and leaves, along with root and shoot size, were analysed using normality tests, parametric, non‐parametric and principal component analysis. The best immersion time for BIT® was eight minutes, resulting in the highest shoot production. When the three systems were compared, partial immersion and semisolid medium systems supported chlorophyll content, root development, and acclimatisation, enhancing plant survival. The BIT® system proved more efficiency for leaf and shoot multiplication due to periodic oxygenation, but it had the lowest acclimatisation percentage. However, with optimal multiplication and a phytohormonal treatment before acclimatisation, plant production for commercial introduction could be achieved. Field introduction was successful in Ixhuatlán del café, Veracruz, México, demonstrating the potential of in vitro techniques for kiwifruit plants production and their cultivation.

The Orchidaceae family is of significant decorative, pharmaceutical, alimentary, and cultural importance worldwide. This family is very vulnerable due to illegal looting, habitat destruction, and climate change. The development of new hybrids helps meet the demand for specimens that possess outstanding appearance, fragrance, and resistance characteristics and may reduce illegal looting. The objective of this research was to investigate the in vitro propagation of the hybrid Rhyncattleanthe Queen Bee JLA 1 (Rth. Queen Bee JLA 1). Shoot induction was performed with germinated seedlings that were 1 cm in length on semi-solid MS medium with different 6-Benzylaminopurine (BAP), 1-Naphthaleneacetic acid (NAA), 3-Indoleacetic acid (IAA), and 3-indolebutyric acid (IBA) concentrations. Micropropagation was conducted using a temporary immersion system (TIS), a liquid continuous immersion system (CIS), and a conventional semi-solid system (SSS). Afterwards, all regenerated seedlings underwent an acclimatization stage. The highest numbers of shoots (7.04) and leaves (14.28) were obtained with the combination of 1.5 mg L⁻¹ BAP and 0.4 mg L⁻¹ NAA, while the addition of 0.4 mg L⁻¹ IBA in combination with 1.5 mg L⁻¹ BAP enhanced the length of stems (2.12 cm) and leaves (1.88 cm). TIS produced the highest number of shoots (15.68), leaves (22.92), stem length (5.94 cm), and number of leaves (3.50) in seedlings analyzed. The combination of growth regulators BAP and NAA together with the temporary immersion system influenced both the development of the vitroplants and their vegetative development after acclimatization of the hybrid Rth. Queen Bee JLA1 orchid.

El limón Persa (Citrus x latifolia) tiene gran relevancia ya que su comercio internacional ha crecido en los últimos años y la superficie cultivada está aumentando, lo que genera mayor demanda de plantas sanas y genéticamente puras. El limón Persa produce frutos con ausencia de semillas. La ausencia de semillas limita la reproducción sexual de este cultivo, la propagación asexual como el cultivo in vitro de tejidos ofrece una excelente alternativa reproductiva. El cultivo in vitro a través de los sistemas de inmersión temporal con biorreactores RITA® promueve la multiplicación de plantas con calidad fitosanitaria. El objetivo de este estudio fue analizar el efecto de los biorreactores RITA®, medio semisólido e inmersión parcial en el cultivo in vitro de limón Persa determinar su desarrollo morfológico, concentración de las clorofilas a, b y total, fitohormonas AIB, BAP y KIN y de nutrimentos N, P, K, Ca, Mg, Fe, Cu, Zn, Mn, Fe y B en tejido vegetal. en Biorreactores RITA® se generó el mayor número de brotes, mayor concentración de BAP y KIN, macronutrimentos N, P y K y de los micronutrimentos Fe, Mn y B, la propagación in vitro de limón Persa en biorreactores RITA® es altamente eficiente.

The main commercial species of coffee is Coffea arabica; however, it is highly susceptible to biotic and abiotic factors. For this reason, scions of Coffea arabica species are used on robusta (Coffea canephora) rootstocks since grafting can modify the organoleptic, nutraceutical, and nutritional characteristics of crops. The objective of this study was to evaluate the effects of five roasting degrees (light, medium light, medium, medium dark, and dark) in two varieties of Coffea arabica species (Colombia Rojo and Costa Rica 95) and their respective grafts on C. canephora variety Romex. The concentrations of acrylamide, caffeine, and chlorogenic acids were analyzed using HPLC, and the nutrient content was analyzed using plasma induction atomic emission spectrophotometry. The graft of the Colombia Rojo variety with the medium dark roast had the highest concentration of caffeine (1.523%). The same variety with the light roast had the highest concentration of chlorogenic acid (3.088 mg g⁻¹), while the highest acrylamide content (336.70 ng g⁻¹) was found in the same variety with the medium light roast. The roasting degree, variety, and grafting of coffee influence the organoleptic, nutraceutical, and nutritional contents of coffee infusions.