Worldwide pesticides are used by farmers for growing more food on less land by protecting crops from pests, diseases and weeds as well as raising productivity per hectare. Also, pesticides enable farmers to produce safe, quality foods at affordable prices. Therefore, the market for agricultural pesticides is anticipated to rise by $ 26.23 billion between 2021 and 2025. Pesticide residues can be discovered in a wide range of common foods and drinks, such as prepared meals, water, wine, fruit juices, snacks, and animal feeds. Furthermore, it should be noticed that chemical pesticides have been linked to a variety of detrimental health consequences, including impacts on the skin, gastrointestinal system, nervous system, respiratory system, reproductive system, and endocrine system. The current review was carried out using an objective mixture of primary and secondary information, including inputs from key participants in pesticides industry. Also, it aims at highlighting the urgent need for a new concept in agriculture involving a drastic reduction in the use of chemical pesticides, driving the market to bio-based pesticides.

Important land surfaces from hill and mountain areas from the northern hemisphere formerly used for cropping were abandoned. Often, the abandoned land evolved by natural succession to grassland, shrubland or even to forest. The main goal of this paper is to bring new datasets necessary for the understanding of the evolution of ex-arable grassland vegetation from the forest steppe area into relationship with climate. The researches were performed in the locality of Grădinari (Caraş-Severin County, Western Romania) on an ex-arable plot abandoned since 1995. The vegetation data were collected for 19 years (time interval 2003–2021). The analyzed vegetation features were floristic composition, biodiversity and pastoral value. The climate data considered were air temperature and rainfall amount. The vegetation and climate data were correlated statistically, with a view to highlighting the potential impact of the temperature and rainfalls during the evolution of succession process on the grassland’s floristic composition, biodiversity and pastoral value. The pressure of the increased temperatures on the natural restoration process of the biodiversity and pastoral value of ex-arable forest steppe grassland could, at least partially, be mitigated by random grazing and mulching works.

This study aims to highlight the therapeutic potential of some Lamiacea essential oils (EOs). For this purpose, eight EOs, including two from Lavandula angustifolia Mill. cultivated in Romania and Spain (LA1 and LA2), Salvia officinalis L. (SO), Lavandula hybrida Balb. ex Ging (LH), Salvia sclarea L. (SS), Mentha smithiana L. (MS), Perovskia atriplicifolia Benth. (PA), and Mentha x piperita L. (MP), were evaluated in vitro in terms of antioxidant, cytotoxic, antimicrobial, and anti-migratory activities. As regards the antioxidant capacity, expressed as the EO concentration that produces 50% of the maximum effect (IC₅₀ value), the EOs obtained from the cultivated plants of the Lamiaceae family are ordered as follows: LA2 ˃ LA1 ˃ LH > MP > MS > SO > SS > PA. For the determination of antimicrobial activity, the reference strains used for testing were Salmonella enterica serotype typhimurium, Shigella flexneri serotype 2b, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, and Candida parapsilosis. The most intense inhibitory effect was observed in EOs of MS and MP on all tested microbial strains. The cytotoxic and anti-migratory activity of EOs was tested on two melanoma cell lines (A375 and B164A5) and on a healthy keratinocyte line (HaCaT). EOs LA1 and MP manifested the highest selectivity on the analysed tumoural cells, by reducing their migration in comparison with the control, proving to have therapeutic potential.

This paper’s aim was to investigate the influence of variety and the climatic year on vegetation phenophases in blueberries grown in southwest Romania, the Banat region. This study was carried out during the growing season of 2020–2022 in a blueberry plantation, for ‘Duke’, ‘Hannah’s Choice’ and ‘Elliott’ varieties. In the study, phenological traits were recorded using the BBCH phenological scale and the observation of phenotypic data was recorded as in Julian days. Thus, it is found that the duration of each phenophase characterized each variety. The calendar periods for the onset of vegetation and the duration of spring phenological development stages in varieties have differed from year to year and depended on weather conditions. In the case of the phenological stage, depending on variety, the maximum amplitude was recorded for BBCH 87 stage (75% blue fruits) of 51 days, and the minimum amplitude, of 25 days, for BBCH 51 stage (bud swell) and BBCH 59 (late pink bud). The coefficient of variation, depending on climatic year, for generative phenophases, had values between 6.5% (BBCH 67-petal fall) and 21.1% (BBCH 51-bud swell). It was found that the variety and the climatic year influence the development of vegetation phenophases. The results indicate that blueberry cultivars have demonstrated a high degree of phenotypic plasticity to respond to gradual changes in environmental conditions and are important for the evaluation of cultivar cultivation prospects in the studied area.

The landscapes and, implicitly, the surfaces of secondary grasslands in the mountain areas have been intensively modified and transformed by humans. In this context, this paper analyses the spatial and temporal changes of grassland surfaces following the impact of human population reduction. Thus, the study proposes the implementation of the Grassland Anthropic Impact Index (GAII) as a “measurement tool” to functionally link the two components, grassland surface and human population. The spatiotemporal analyses are based on Corine Land Cover data and demographic data, processed via Geographic Information Systems (GIS) methods and the Land Change Modeler (LCM) tool. The research shows that over a period of 28 years, the population, which was continuously decreasing, caused a series of transformations to the grasslands over an area of 33343 ha. The influence of the reduction in the number of inhabitants was also demonstrated by the direction of the changes produced in the grassland surfaces: in the better populated areas, the grasslands expanded over lands with other uses, and in the sparsely populated areas, they were abandoned. GAII values generally increase with the decrease of the population in the target area, meaning that for an inhabitant (potential user) a greater grassland surface is reported, resulting in a greater responsibility for the management of this resource on a space and time scale. Following the evaluation of the trend of the last 28 years, it was observed that the depopulation of mountain areas can be seen as a threat to grassland ecosystems, either through the transition to other categories of use, or through abandonment. The implications of these phenomena are much broader: they produce chain reactions and affect other components of the regional geosystem.

Fusarium graminearum (Fusarium Head Blight - FHB) is a dangerous pathogen of the cereals producing mycotoxins harmful for human and animal health. The quality of the wheat grains is severely affected in the years with FHB epidemics due to the high mycotoxins level that can overpass maximum allowable concentrations, with implications for food safety. The purpose of the work was to investigate some variables involved in the contamination of the wheat grains with several harmful mycotoxins (DON, ZON and T-2) in conditions of FHB natural infection in an epidemic year. The main finding of the results highlighted that the low ears densities of wheat crop canopy determinate high FHB attack frequency, too. Climate conditions from May 2019 were very favourable for the evolution of FHB epidemics in western Romania. This fact was confirmed by the experimental results of FHB attack, and later by the concentrations of mycotoxins (DON) over the maximum allowable concentrations from the wheat grains at the harvesting moment. The overpass of DON concentration was found in 15 from the total 16 wheat varieties from the experiment. Positive significant correlations were found between DON and T-2 and between DON and fungus attack intensity. According with the results, in FHB epidemic year some of the most influential factors in the contamination of the wheat grains with DON, ZON and T-2 in the order of their importance are: ears density, frequency of the attack on ear, number of diseased ears and attack intensity on ears