The article presents the data on immunological and morphological blood indicators of the newborn piglets. In the experiment, 20 sows of the 3rd-4th farrowing and the offspring obtained from them were selected. During the farrowing period, the piglets underwent clinical examination and weighing. The animals that did not reach 900 g were counted as hypotrophic piglets, the animals over 900 g – normotrophic, respectively. The first group (n=10) - hypotrophic piglets, and the second (n=10) - normotrophic piglets. Morphological blood examination in the hypotrophic piglets showed a decrease in the concentration of hematocrit and hemoglobin. The mean cell hemoglobin (MCH) assumed the values at the upper limit of the physiological norm, while the mean cell hemoglobin concentration (MCHC) in the normotrophics was by 17% higher than in the hypotrophics (p<0.05). The red cell distribution width (RDW) in the normotrophics was by 2.3 times higher than in the hypotrophics (p<0.05). When studying the immunological status of piglets, it was noted that the serum lysozyme activity in the hypotrophics was by 16% lower than in the normotrophics. The PAL in the normotrophics was 79.6±1.58, which was by 4.8% higher than in the hypotrophics (p<0.05). The PhN in the hypotrophics was by 11% less than in the normotrophics (p<0.05). The phagocytic index in the normotrophics was by 10% higher than in the hypotrophics (4.68±0.16) (p < 0.05). The PR of the normotrophics was by 1.5 times higher than that of the hypotrophics and was 3.03±0.2 (p<0.05).

The state of the immunobiochemical status of the blood of cows when using a drug containing gm-csf was studied on 15 animals with a hypofunctional state of the ovaries. The animals were divided according to the principle of analogues into three groups. The animals were included in the experiment 45- 60 days after calving without signs of sexual cyclicity. The cows of the first group were injected with a drug based on gm-csf (granulocyte-macrophage colony-stimulating factor), the cows of the second one - follimag, the third group served as a negative control without the introduction of drugs. Evaluation of the efficacy of the use of gonadotropic and recombinant drugs showed that the use of the agent based on gm-csf turned out to be the most effective in comparison with follimag and the negative control. After the use of the drug of recombinant interferons, the content of circulating immune complexes turned out to be lower by 30.9%, malon dialdehyde - by 17.1%, medium molecular peptides - by 34.9% TNFα - by 21.0%, IL-1β - by 41.1%, IL-2 - by 35.9%, with an increase in serum bactericidal and lysozyme activity by 13.8% and 21.7%, respectively, phagocytic index - by 12.9%, phagocytic number - by 18.4%, progesterone - by 5.09 times, estradiol - by 29.3%, dehydroepiandrosterone sulfate - by 78.9%, IL-4 - by 44.6%, IL-10 - by 20.3%, which indicates activation the humoral link of general nonspecific resistance, an increase in the content of anti-inflammatory cytokines, with a decrease in the intensity of lipid peroxidation processes, the level of proinflammatory cytokines against the background of the restoration of the hormoneproducing function of the sex gonads. Changes in the indicators of the immunobiochemical blood status of cows are reflected in the activation of the functional activity of the ovaries, the manifestation of full-fledged sexual cycles by animals and successful insemination.

The article presents the results of studying the efficacy of the drug based on granulocyte-macrophage colony-stimulating factor (GM-CSF) in cows with postpartum ovarian hypofunction. It has been established that its twofold administration at a dose of 10.0 ml/animal with a 7-day interval ensures the restoration of sexual cyclicity in 90.0% of cows with a reduction in the length of infertility by 18.0-30.1 days and the fertilization coefficient – by 0.56-0.78. A twofold use of the drug based on GM-CSF at a dose of 10.0 ml is accompanied by the development of dominant follicles, the diameter of which is by 46.6% larger compared to saline, an increase in the level of DHEA-S by 2.0 times, estradiol-17β – by 29.3%, which ensures a full manifestation of sexual cyclicity.

The study presents the data on the level of expression of pro- and anti-inflammatory cytokines in piglets with gas-trointestinal diseases of bacterial etiology. The analysis of gene expression showed that the expression levels of IL-1α and IL-10 were significantly increased in sick piglets, while IL-1β lost its activity. During the histological examination, there were no differences between the structure of the intestines of sick and healthy piglets. However, cytological examination: of the samples obtained from sick piglets demonstrated alteration and a decrease in the adhesive barrier in the epithelium of intestinal villi.