A wireless and battery-less trimodal neural interface system-on-chip (SoC), capable of 16-ch neural recording, 8-ch electrical stimulation, and 16-ch optical stimulation, all integrated on a 5 × 3 mm ² chip fabricated in 0.35-μm standard CMOS process. The trimodal SoC is designed to be inductively powered and communicated. The downlink data telemetry utilizes on-off keying pulse-position modulation (OOK-PPM) of the power carrier to deliver configuration and control commands at 50 kbps. The analog front-end (AFE) provides adjustable mid-band gain of 55-70 dB, low/high cut-off frequencies of 1-100 Hz/10 kHz, and input-referred noise of 3.46 μV _rms within 1 Hz-50 kHz band. AFE outputs of every two-channel are digitized by a 50 kS/s 10-bit SAR-ADC, and multiplexed together to form a 6.78 Mbps data stream to be sent out by OOK modulating a 434 MHz RF carrier through a power amplifier (PA) and 6 cm monopole antenna, which form the uplink data telemetry. Optical stimulation has a switched-capacitor based stimulation (SCS) architecture, which can sequentially charge four storage capacitor banks up to 4 V and discharge them in selected μLEDs at instantaneous current levels of up to 24.8 mA on demand. Electrical stimulation is supported by four independently driven stimulating sites at 5-bit controllable current levels in ±(25-775) μA range, while active/passive charge balancing circuits ensure safety. In vivo testing was conducted on four anesthetized rats to verify the functionality of the trimodal SoC.

An inductively-powered wireless integrated neural recording and stimulation (WINeRS-8) system-on-a-chip (SoC) that is compatible with the EnerCage-HC2 for wireless/battery- less operation has been presented for neuroscience experiments on freely behaving animals. WINeRS-8 includes a 32-ch recording analog front-end (AFE), a 4-ch current-controlled stimulator, and a 434 MHz on-off keying (OOK) data link to an external software- defined radio (SDR) wideband receiver (Rx). The headstage also has of a Bluetooth Low Energy (BLE) link for controlling the SoC. WINeRS-8/EnerCage-HC2 systems form a bidirectional wireless and battery-less neural interface within a standard homecage, which can support longitudinal experiments in an enriched environment. Both systems were verified in vivo on rat animal model, and the recorded signals were compared with hardwired and battery-powered recording results. Real-time stimulation and recording verified the system's potential for bidirectional neural interfacing within the homecage, while continuously delivering 35 mW to the hybrid WINeRS-8 headstage over an unlimited period.

Distributed neural interfaces made of many mm-sized implantable medical devices (IMDs) are poised to play a key role in future brain-computer interfaces because of less damage to the surrounding tissue. Evaluating them wirelessly at preclinical stage (e.g., in a rodent model), however, is a major challenge due to weak coupling and significant losses, resulting in limited power delivery to the IMD within a nominal experimental arena, like a homecage, without surpassing the specific absorption rate limit. To address this problem, we present a dual-band EnerCage system with two multi-coil inductive links, which first deliver power at 13.56 MHz from the EnerCage (46 × 24 × 20 cm ³ ) to a headstage (18 × 18 × 15 mm ³ , 4.8 g) that is carried by the animal via a 4-coil inductive link. Then, a 60 MHz 3-coil inductive link from the headstage powers up the small IMD (2.5 × 2.5 × 1.5 mm ³ , 15 mg), which in this case is a free floating, wirelessly powered, implantable optical stimulator (FF-WIOS). The power transfer efficiency and power delivered to the load (PDL) from EnerCage to the headstage at 7 cm height were 14.9%-22.7% and 122 mW; and from headstage to FF-WIOS at 5 mm depth were 18% and 2.7 mW, respectively. Bidirectional data connectivity between EnerCage-headstage was established via bluetooth low energy. Between headstage and FF-WIOS, on-off keying and load-shift-keying were used for downlink and uplink data, respectively. Moreover, a closed-loop power controller stabilized PDL to both the headstage and the FF-WIOS against misalignments.

This paper presents a mm-sized, free-floating, wirelessly powered, implantable optical stimulation (FF-WIOS) device for untethered optogenetic neuromodulation. A resonator-based three-coil inductive link creates a homogeneous magnetic field that continuously delivers sufficient power (>2.7 mW) at an optimal carrier frequency of 60 MHz to the FF-WIOS in the near field without surpassing the specific absorption rate limit, regardless of the position of the FF-WIOS in a large brain area. Forward data telemetry carries stimulation parameters by on-off-keying the power carrier at a data rate of 50 kb/s to selectively activate a 4 × 4 μLED array. Load-shift-keying back telemetry controls the wireless power transmission by reporting the FF-WIOS received power level in a closed-loop power control mechanism. LEDs typically require high instantaneous power to emit sufficient light for optical stimulation. Thus, a switched-capacitor-based stimulation architecture is used as an energy storage buffer with one off-chip capacitor to receive charge directly from the inductive link and deliver it to the selected μLED at the onset of stimulation. The FF-WIOS system-on-a-chip prototype, fabricated in a 0.35-μm standard CMOS process, charges a 10-μF capacitor up to 5 V with 37% efficiency and passes instantaneous current spikes up to 10 mA in the selected μLED, creating a bright exponentially decaying flash with minimal wasted power. An in vivo experiment was conducted to verify the efficacy of the FF-WIOS by observing light-evoked local field potentials and immunostained tissue response from the primary visual cortex (V1) of two anesthetized rats.

Integrative neural interfaces combining neurophysiology and optogenetics with neural imaging provide numerous opportunities for neuroscientists to study the structure and function of neural circuits in the brain. Such a comprehensive interface demands miniature electrode arrays with high transparency, mechanical flexibility, electrical conductivity, and biocompatibility. Conventional transparent microelectrodes made of a single material, such as indium tin oxide (ITO), ultrathin metals, graphene and poly-(3,4-ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT:PSS), hardly possess the desired combination of those properties. Herein, ultra-flexible, highly conductive and fully transparent microscale electrocorticogram (μECoG) electrode arrays made of a PEDOT:PSS–ITO–Ag–ITO assembly are constructed on thin parylene C films. The PEDOT:PSS–ITO–Ag–ITO assembly achieves a maximum ∼14% enhancement in light transmission over a broad spectrum (350–650 nm), a significant reduction in electrochemical impedance by 91.25%, and an increase in charge storage capacitance by 1229.78 μC cm⁻². Peeling, bending, and Young's modulus tests verify the enhanced mechanical flexibility and robustness of the multilayer assembly. The μECoG electrodes enable electrical recordings with high signal-to-noise ratios (SNRs) (∼35–36 dB) under different color photostimulations, suggesting that the electrodes are resilient to photon-induced artifacts. In vivo animal experiments confirm that our array can successfully record light-evoked ECoG oscillations from the primary visual cortex (V1) of an anesthetized rat.

Objective . We have developed a wireless opto-electro interface (WOENI) device, which combines electrocorticogram (ECoG) recording and optical stimulation for bi-directional neuromodulation on small, freely behaving animals, such as rodents. Approach . The device is comprised of two components, a detachable headstage and an implantable polyimide-based substrate. The headstage establishes a bluetooth low energy (BLE) bi-directional data communication with an external custom-designed USB dongle for receiving user commands and optogenetic stimulation patterns, and sending digitalized ECoG data. Main results . The functionality and stability of the device were evaluated in vivo on freely behaving rats. When the animal received optical stimulation on the primary visual cortex (V1) and visual stimulation via eyes, spontaneous changes in ECoG signals were recorded from both left and right V1 during four consecutive experiments with 7 d intervals over a time span of 21 d following device implantation. Immunostained tissue analyses showed results consistent with ECoG analyses, validating the efficacy of optical stimulation to upregulate the activity of cortical neurons expressing ChR2. Significance . The proposed WOENI device is potentially a versatile tool in the studies that involve long-term optogenetic neuromodulation.