In the present study, the potential of elemental analysis combined with statistical tools to identify honey origin was evaluated by mineral characterization of 173 honeys of 13 floral types (acacia, fir, spruce, linden, chestnut, lavender, coriander, thistle, honeydew, rosemary, sage, euphorbia and ziziphus plant species) collected from five geographical regions (Slovenia, Croatia, Bulgaria, Turkey, and Morocco). The objective of the study was to accurately and reliably differentiate the mineral composition among honey varieties. The aim was to establish traceability, to ensure product authenticity and to improve quality control measures within the honey industry. For this purpose, 18 major, minor and trace elements were quantified using microwave digestion, followed by ICP-MS measurement. Statistical evaluation of elemental concentration was undertaken using principal component analysis (PCA) to distinguish honey floral types. The research give light on the specific elements that can serve as indicators for determining the geographical and botanical source of honey. Our findings indicate that certain elements, such as Mn, K, and Ca, are primarily influenced by the type of pollen present in the honey, making them indicative of the floral source. On the other hand, levels of Na, Mg, and Fe were found to be more strongly influenced by environmental factors and can be considered as markers of geographical origin. One novel aspect of this research is the exploration of the relationship between honey minerals and honey botanical source. This was achieved through the analysis of chestnut tree samples and a subsequent comparison with the composition of chestnut honey.

Pollution by heavy metals is one of the most serious issues in the world, and the principal sources are manufacturing, fertilizers, mining, pesticides, transport, and wastewater discharge. In this study, the presence of macroelements (Nitrogen (N) and Phosphorus (P)) and heavy metals (Chromium (Cr), Nickel (Ni), Cadmium (Cd), Iron (Fe), Zinc (Zn), Lead (Pb), Cooper (Cu), and Manganese (Mn)) in the green tide caused by the green seaweed Ulva lactuca from Marchica lagoon (NE-Morocco, Mediterranean) was evaluated. The mean values of P and N in the algae were 1773.33 mg·kg ⁻¹ and 44.4 g·kg ⁻¹, respectively. The heavy metals have mean values following this descending sequence: Fe > Mn > Zn > Cu > Ni > Pb > Cr > Cd. This finding has suggested that Ulva has the strongest affinity to Fe and Mn among the metals examined. Mn, Fe, and Pb recorded the highest bioconcentration factors (BCFs), which were 1687, 1656, and 1643, respectively. Regarding their contribution to the Recommended Dietary Allowance (RDA), Fe showed a contribution of 13.58% to the RDA, while that of Mn varied between 4.42 % and 28.42%. The health risk assessment did not indicate any hazard related to the ingestion of Ulva lactuca. Therefore, this study suggests that Ulva lactuca in the Nador lagoon has the potential to bioaccumulate heavy metals and mitigate eutrophication.

Microgreens are receiving increasing attention due to their high content of bioactive components and their importance to human health. These emerging food products can be obtained from the seeds of different plant species, including aromatic herbs. Aromatic microgreens are gaining popularity as new functional food products. In this study, we investigated the effects of different light-emitting diode (LED) lamp spectra on the growth, pigments, nitrates, and osmoprotectant content of microgreens of Ocimum basilicum L., Trigonella foenum-graecum, Anethum graveolens, and Anthriscus cerefolium plants. Three types of artificial LED lamps were used: T0 as artificial white light, T1 as a continuous light-emitting diode with a longer blue wavelength, and T2 as a continuous light-emitting diode with a longer red wavelength. The results obtained showed that the three types of LED light had significant effects on the different parameters studied. In relation to growth parameters, such as fresh weight (FW) and microgreen height (H), the T2 treatment was most effective for fenugreek, dill, and chervil. However, in basil plants, FW and H values were higher under T1 treatment. Regarding nitrate accumulation, both T1 and T2 treatments reduced the content of this nutrient in the different species studied here. Finally, levels of chlorophyll, carotenoid, glucose, proline, and proteins were all higher in plants cultivated under T1 and T2 treatments than in control plants.

There is an increasing tendency to replace conventional agricultural plastic mulching films with biodegradable alternatives. However, while the latter biodegrade well under controlled conditions (e.g. industrial compost), their biodegradation in non-target environments (e.g. aquatic environments) is questioned and poorly understood. Therefore, in this study, microplastics derived from conventional polyethylene (PE) and biodegradable polybutylene adipate terephthalate starch blend (PBAT) mulching films were exposed to UV irradiation and subsequently tested for their ready biodegradability in an aqueous medium where changes in their characteristics were evaluated. The results showed limited biodegradation for pristine and UV-aged PE: no morphological, surface chemical or internal changes were observed. Pristine PBAT showed signs of initial biodegradation, while UV-aged PBAT biodegraded by up to 57%. New functional groups appeared on the PBAT surface after UV irradiation according to FTIR analysis and crystallinity increased after biodegradation. Elemental analysis revealed a range of metals in PE and PBAT microplastics. No changes in metal distribution analysed in microplastic after UV-aging or biodegradation were found, except that less titanium was present in PBAT after biodegradation indicating potential leaching. None of the PBAT microplastics had ecotoxic effects towards the aquatic plant Lemna minor. Pristine and UV-aged PE showed negative effects on roots, but these were not observed after biodegradation. Low biodegradation of pristine PBAT and possible leaching of metals demonstrated here raise questions about the sustainable use of biodegradable alternatives, especially when they enter non-target environments.

Sedimentation is a naturally occurring process of allowing particles in water bodies to settle out of the suspension under a gravity effect. In this study, the sediments of the Drava River were fully investigated to determine the heavy metal concentrations along the river and their potential reuse in the construction sector. Naturally dehydrated sediments from the Drava River were tested as an additive for the production of fired bricks. The dredged sediments were used as a substitute for natural brick clay in amounts up to 50% by weight, and it was confirmed that up to 20% by weight of the added sediment could be used directly in the process without critically affecting performance. Finally, the naturally dehydrated sediments were also evaluated for their use as a filling material in the construction of levees. The natural moisture content of the dehydrated sediment was too high for it to be used without additives, so quicklime was added as an inorganic binder. The test results showed an improvement in the geotechnical properties of the material to such an extent that it is suitable as a filling material for levees.

Knowing the fate of nanoplastics in organisms is indispensable to assess the risk of these persistent environmental pollutants. We designed an in-depth study on the biodistribution of metal (europium)-doped polystyrene nanoparticles (EuPSs) in terrestrial isopods (Crustacea, Porcellio scaber) upon dietary exposure. Biodistribution of two types of spherical EuPSs, 100 nm and 300 nm, with similar Eu content and negligible dissolution of Eu ions in deionized water, was investigated. In the first step, the total Eu concentration in different body regions (gut, digestive glands and rest of the body) was measured with inductively coupled plasma mass spectrometry (ICP-MS). Next, tissue localisation of the particles was analysed using X-ray microtomography, fluorescent microscopy and laser ablation ICP-MS. In addition, scanning electron microscopy was used to check the presence of the particles in the digestive system. As the measured levels of Eu in body parts other than the gut, including the digestive gland, are negligibly low, we conclude that EuPSs do not accumulate in the body of P. scaber following dietary exposure. Based on our findings, we recommend complementing analytical and microscopic techniques with detailed physiological and anatomical knowledge of the investigated organisms before drawing any conclusions on body distribution.