Our country is home to many endemic plant species including medicinal, aromatic and spice. One of these species is thyme plant used both in daily life and for medical purposes. One of the most popular methods of phylogenetic classification of plants is the internal transcribed spacer (ITS) region which locates between the ITS1 and ITS2 regions that highly conserved. In this paper, a thyme species was used, which grows naturally in Sultan Murat Sarıkaya High-plateau in eastern Black Sea Region (Turkey) and phylogenetic analysis of oregano plant was carried out and antioxidant and antibacterial activity was investigated. The assessment of the antioxidant activity of the plant was researched using DPPH method using plant excretes prepared at various time intervals. The antibacterial activity of plant was studied by using disc diffusion and minimum inhibitory concentration (MIC) methods against Gram (+) and Gram (-) bacteria. The phylogenetic analysis was performed by obtaining the genomic DNA of the plant by the analysis of the specific DNA sequences used in the species identification, and the species of the plant was identified as Origanum vulgare and was identified as MH174928.1 (Origanum vulgare isolate SRY61) from Gen Bank. O. vulgare showed a high rate of antioxidant properties and showed antibacterial effect in accordance with the literature.

Tomato crop residues (TCR) from soilless greenhouses are treated as waste, causing greenhouse gas emissions and biomass loss. Within a circular economy framework, gasification converts TCR into renewable energy and biochar; however, its high pH and electrical conductivity (EC) limit its use as a substrate. This study evaluated whether pre-treatment could enable TCR biochar to act as a substrate component and nutrient source in tomato and pepper seedlings. Biochar was produced by gasification and pre-treated by water incubation plus nitric acid, reducing EC from 27 to 8.7 dS m⁻¹ and pH from 10.4 to 8.2 while achieving nitrate loading without leaching. Pristine biochar severely restricted growth. Subsequent experiments evaluated pre-treated biochar mixed with perlite or cocopeat, with or without external N and K. The 15/85% (w/w) pre-treated biochar/cocopeat mixture (PTB/C) showed the best overall performance. In the absence of additional N/K, PTB/C produced shoot biomass and shoot N concentrations comparable to N-/K-supplemented cocopeat; shoot K was comparable in tomato and higher in pepper. With N and K supplementation, PTB/C exceeded supplemented cocopeat biomass by 1.41- and 1.95-fold in tomato and pepper, respectively. These results indicate that pre-treated TCR biochar can reduce dependence on imported cocopeat and external N/K supply.

Amaç: Bu çalışmada Gümüşhane ilinde doğal olarak yetişen kuşburnu meyvelerinin hem kabuk hem de çekirdeğinin (tohum) ultrasonik destekli ekstraksiyon (UAE) yöntemi kullanılarak hazırlanan etanol ekstraktlarının 2,2-diphenyl-1-picryl-hydrazil (DPPH) metodu ile antioksidan aktivitelerinin kıyaslanması amaçlanmıştır.Yöntem: Çalışmada kuşburnu meyvesinin kabuk ve çekirdeğine ait kısımlarından farklı zaman aralıklarında UAE yöntemi kullanılarak hazırlanmış olan ekstraktların antioksidan aktivitesi 2,2-diphenyl-1-picryl-hydrazil (DPPH) metodu ile belirlenmiştir.Bulgular: 2 dakika süreyle UAE destekli ekstraksiyona ait kuşburnu ekstraktlarının serbest radikal yakalama aktivitesinin hem kabuk hem de çekirdek için IC50 değeri sırasıyla 0.69±0.005 mg mL-1 ve 0.39±0.0007 mg mL-1 olarak hesaplanmıştır. Kuşburnu meyvesine ait çekirdek kısmının kabuk kısmına göre daha yüksek bir radikal süpürücü aktiviteye sahip olduğu bulunmuştur.Sonuç: Elde edilen verilerin literatürde mevcut bulunan verilere göre daha etkili olduğu sonucuna varılmış olup bunun kullanılan ekstraksiyon yöntemi ile ilişkili olduğu düşünülmektedir.

The use of chemical pesticides is limited by several public health concerns regarding their toxicity levels and indiscriminate use. Nevertheless, they are still vital components of agricultural industry since no other competitive equivalents to chemical pesticides still exist in terms of efficiency. This study describes the preparation and biological assessment of an insecticide releasing plastic film for agricultural covering purposes. The formulation was prepared by incorporation of deltamethrin loaded, nano-sized halloysite nanotubes into polymeric films. Thermal, morphological, and mechanical properties of films were characterized by Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM) and Universal Testing Machine UTM. Sustained release profiles of the films were evaluated by Thermogravimetric Analysis (TGA). Results reveal that deltamethrin was successfully loaded into halloysite nanotubes and nanotube incorporation enhances the elastic modulus of linear-low density polyethylene (LLDPE) films. In addition, films exhibit controlled release function of the active agent for 32 days. Bioassays of the nanocomposite films with varying deltamethrin doses tested on grasshoppers showed that the LD ₅₀ values of the films are 1.85 x 10 ⁻⁵ g/cm ² . Insecticidal activities of films were tested in greenhouse on Medicago Sativa plants contaminated with thrips and aphid. Nanocomposites are observed to repel mature aphids and kill young aphids and thrips.