Uiara Maria de Barros Lira Lins

Mrs. Uiara Maria de Barros Lira Lins


Research Focus & Metrics

Academic Career Length

3Years (2024-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

View All Publications
18 July 2024 in Fermentation
Verified
Journal Article
Open access
PDF file
Uiara Maria de Barros Lira LinsUiara Maria de Barros Lira Lins
Uiara Maria de Barros Lira Lins
RMRafael de. Souza Mendonça
Rafael de. Souza Mendonça
SDSérgio S. S. Dantas
Sérgio S. S. Dantas
ASAdriana Ferreira de Souza
Adriana Ferreira de Souza
Dayana Montero RodríguezDayana Montero Rodríguez
Dayana Montero Rodríguez
GTGalba Maria De Campos Takaki
Galba Maria De Campos Takaki
Simvastatin, a semisynthetic drug widely used to lower cholesterol, is among the most prescribed statins worldwide. This study focuses on the direct production of a simvastatin-like biomolecule using alternative substrates by Aspergillus spp. strains. Two species, A. terreus UCP 1276 and A. flavus UCP 0316, were initially evaluated in synthetic media as control. Subsequently, the carbon and nitrogen sources were replaced by agro-industrial substrates, resulting in five modified media. Cultures were maintained at 28 °C, pH 6.5, at 180 rpm for 21 days. Fungal growth kinetics were evaluated and a 23 full-factorial design (FFD) was used to investigate the influence of substrate concentration on statin yield. Presence of inhibitors was confirmed by bioassay, UV–visible spectrophotometry, and thin-layer chromatography (TLC). According to the results, A. flavus UCP yielded 0.24 mg/g of statin in condition 2 of FFD (medium containing 4.5% soluble starch and saline base), suggesting it as a promising candidate for direct production of the biomolecule. Statistical analysis showed the significant effect of soluble starch on inhibitor production, making it a viable and profitable alternative substrate. Moreover, the isolated statin exhibited broad-spectrum antimicrobial activity, including efficacy against Gram-negative and Gram-positive bacteria and yeasts, indicating therapeutic potential against antimicrobial resistance.