University of Thessaly

Coffee belongs among the most widespread beverages worldwide, and its increasing consumption leads to the generation of a large amount of by-products, mainly spent coffee grounds (SCGs). SCGs can be further recycled as they contain bioactive compounds. This research aims to search for the extraction conditions that lead to the highest recovery of these compounds through a green extraction technique, pressurized liquid extraction (PLE). The parameters studied were solvent composition, temperature, and extraction time, while the pressure was kept constant at 1700 psi. The optimum conditions were 48% v/v ethanol solution at 160 °C for 25 min. Under these conditions, the maximum recoveries were total polyphenol content of 15.99 mg gallic acid equivalents/g dry weight (dw), a total caffeine content of 1.15 mg/g dw, and an antioxidant capacity determined through the ferric-reducing antioxidant power of 101.87 μmol ascorbic acid equivalents/g dw. Additionally, individual polyphenols in SCGs were studied by high-performance liquid chromatography, revealing that the extracts were rich in chlorogenic acid, (−)-epicatechin, and rutin. The results of this work can set the basis for the further utilization of SCGs through PLE by the beverage, food, pharmaceutical, and cosmetic industries.

Athanasios Christoforidis; Martha Mantiniotou; Vassilis Athanasiadis; Stavros I. Lalas. Caffeine and Polyphenolic Compound Recovery Optimization from Spent Coffee Grounds Utilizing Pressurized Liquid Extraction. Beverages 2025, 11, 74 .

Athanasios Christoforidis, Martha Mantiniotou, Vassilis Athanasiadis, Stavros I. Lalas. Caffeine and Polyphenolic Compound Recovery Optimization from Spent Coffee Grounds Utilizing Pressurized Liquid Extraction. Beverages. 2025; 11 (3):74.

Athanasios Christoforidis; Martha Mantiniotou; Vassilis Athanasiadis; Stavros I. Lalas. 2025. "Caffeine and Polyphenolic Compound Recovery Optimization from Spent Coffee Grounds Utilizing Pressurized Liquid Extraction." Beverages 11, no. 3: 74.

Periklis Marnas; Stefan Lüpold; Lydia Giannakou; Athanasios-Stefanos Giannopoulos; Chrissi Hatzoglou; Konstantinos I Gourgoulianis; Sotirios G Zarogiannis; Erasmia Rouka. Modeling COPD in Drosophila melanogaster by cigarette smoke inhalation: Functional changes and alterations in the expression of COPD-relevant orthologous genes. 2025 .

Periklis Marnas, Stefan Lüpold, Lydia Giannakou, Athanasios-Stefanos Giannopoulos, Chrissi Hatzoglou, Konstantinos I Gourgoulianis, Sotirios G Zarogiannis, Erasmia Rouka. Modeling COPD in Drosophila melanogaster by cigarette smoke inhalation: Functional changes and alterations in the expression of COPD-relevant orthologous genes. . 2025; ():.

Periklis Marnas; Stefan Lüpold; Lydia Giannakou; Athanasios-Stefanos Giannopoulos; Chrissi Hatzoglou; Konstantinos I Gourgoulianis; Sotirios G Zarogiannis; Erasmia Rouka. 2025. "Modeling COPD in Drosophila melanogaster by cigarette smoke inhalation: Functional changes and alterations in the expression of COPD-relevant orthologous genes." , no. : .

The aim of this study was to evaluate the technical and economic impact of using commercial phytogenic feed supplements and dried Greek Oregano leaves as feed additives on dairy sheep farms. Fifteen farms in the Greek region of Thessaly were divided into intervention and control farms, and techno-economic data were collected before and after supplementation through structured interviews and cost analysis. The results showed that the administration of certain phytogenic supplements and oregano to ewes resulted in improved animal health, higher milk yield, and lower production costs, which created a positive trend in the financial results of the farm. Further research is needed to accurately determine the ideal production stage of the animals for the interventions, the amount of supplements administered, and the selection of appropriate plant species, which would lead to better financial management of the farms.

Maria Spilioti; Konstantinos Tousis; Georgios Papakonstantinou; Eleftherios Meletis; Alexis Manouras; Eleftherios Nellas; Garyfalia Economou; Vasileios G. Papatsiros; Konstantinos Tsiboukas. Techno-Economic Analysis of Innovative Phytogenic-Based Supplements for Ruminant Health and Productivity. Agriculture 2025, 15, 1090 .

Maria Spilioti, Konstantinos Tousis, Georgios Papakonstantinou, Eleftherios Meletis, Alexis Manouras, Eleftherios Nellas, Garyfalia Economou, Vasileios G. Papatsiros, Konstantinos Tsiboukas. Techno-Economic Analysis of Innovative Phytogenic-Based Supplements for Ruminant Health and Productivity. Agriculture. 2025; 15 (10):1090.

Maria Spilioti; Konstantinos Tousis; Georgios Papakonstantinou; Eleftherios Meletis; Alexis Manouras; Eleftherios Nellas; Garyfalia Economou; Vasileios G. Papatsiros; Konstantinos Tsiboukas. 2025. "Techno-Economic Analysis of Innovative Phytogenic-Based Supplements for Ruminant Health and Productivity." Agriculture 15, no. 10: 1090.