Natalia Pachura-Hanusek

Dr. Natalia Pachura-Hanusek

Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Norwida 25, 50-375 Wrocław, Poland
orcid

Research Focus & Metrics

Academic Career Length

7Years (2020-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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13 August 2020 in Foods
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AKAndrzej Kwaśnica
Andrzej Kwaśnica
NPNatalia Pachura-Hanusek
Natalia Pachura-Hanusek
Klaudia MasztalerzKlaudia Masztalerz
Klaudia Masztalerz
Adam FigielAdam Figiel
Adam Figiel
AZAleksandra Zimmer
Aleksandra Zimmer
HRHenryk Różański
Henryk Różański
Flowers of hemp (Cannabis sativa L.) are widely used in cosmetics, food, and in the pharmaceutical industry. The drying process plays a key role in retention of aroma and also in the quality of products. Seven variants of hemp flower drying, including convection drying (CD), vacuum–microwave drying (VMD), and combined drying consisting of convective pre-drying followed by vacuum–microwave finishing drying (CPD-VMFD) were checked in this study. For each process, we applied the two-term model. Dried material was submitted to color and chromatographical assessments. Analyses of obtained essential oil showed the presence of 93 volatile compounds, predominantly β-myrcene, limonene, and β-(E)-caryophyllene, as well as α-humulene. Application of 240 W during VMD and 50 °C during CD gave the highest retention of aroma compounds, amounting to 85 and 76%, respectively, but with huge color changes. Additionally, sensory analysis proved that drying with a microwave power of 240 W provides a product most similar to fresh material.

22 May 2020 in Molecules
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JWJoanna Wezgowiec
Joanna Wezgowiec
AWAnna Wieczynska
Anna Wieczynska
WWWlodzimierz Wieckiewicz
Wlodzimierz Wieckiewicz
Julita KulbackaJulita Kulbacka
Julita Kulbacka
JSJolanta Saczko
Jolanta Saczko
KWKazimiera A. Wilk
Kazimiera A. Wilk
The purpose of this study was to compare the chemical composition and biological properties of Polish propolis. Ethanol, ethanol-hexane, hexane and hexane-ethanol extracts of propolis from three different regions of Poland were prepared. On the basis of the evaluation of their chemical composition as well as the extraction yield and free radical scavenging activity, the ethanol and hexane-ethanol extractions were proposed as the most effective methods. Subsequently, the biological properties of the extracts were evaluated to investigate the selectivity of an anticancer effect on tongue cancer cells in comparison to normal gingival fibroblasts. The obtained products demonstrated anticancer activity against tongue cancer cells. Additionally, when the lowest extract concentration (100 µg/mL) was applied, they were not cytotoxic to gingival fibroblasts. Finally, a possible anti-inflammatory potential of the prepared products was revealed, as reduced mitochondrial activity and proliferation of macrophages exposed to the extracts were observed. The results obtained indicate a potential of Polish propolis as a natural product with cancer-selective toxicity and anti-inflammatory effect. However, further studies are still needed to thoroughly explain the molecular mechanisms of its action and to obtain the promising health benefits of this versatile natural product.

14 September 2020 in Molecules
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KPKatarzyna Pacyga
Katarzyna Pacyga
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
Izabela MichalakIzabela Michalak
Izabela Michalak
Anita BiesiadaAnita Biesiada
Anita Biesiada
Antoni Jacek SzumnyAntoni Jacek Szumny
Antoni Jacek Szumny
UPUrszula Piszcz
Urszula Piszcz
The use of higher plants for the production of plant growth biostimulants is receiving increased attention among scientists, farmers, investors, consumers and regulators. The aim of the present study was to examine the possibility of converting plants commonly occurring in Europe (St. John’s wort, giant goldenrod, common dandelion, red clover, nettle, valerian) into valuable and easy to use bio-products. The biostimulating activity of botanical extracts and their effect on the chemical composition of celeriac were identified. Plant-based extracts, obtained by ultrasound-assisted extraction and mechanical homogenisation, were tested in field trials. It was found that the obtained formulations increased the total yield of leaves rosettes and roots, the dry weight of leaves rosettes and roots, the content of chlorophyll a + b and carotenoids, the greenness index of leaves, the content of vitamin C in leaves and roots. They mostly decreased the content of polyphenols and antioxidant activities in leaves but increased them in roots and conversely affected the nitrates content. Extracts showed a varied impact on the content of micro and macroelements, as well as the composition of volatile compounds and fatty acids in the celeriac biomass. Due to the modulatory properties of the tested products, they may be used successfully in sustainable horticulture.

17 June 2021 in Frontiers in Plant Science
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KPKatarzyna Pacyga
Katarzyna Pacyga
PPPaweł Pacyga
Paweł Pacyga
IMIzabela Michalak
Izabela Michalak
ABAnita Biesiada
Anita Biesiada
ASAntoni Szumny
Antoni Szumny
UPUrszula Piszcz
Urszula Piszcz
The modern agricultural sector faces the challenge of addressing the needs of the fast-growing global population. This process should be both high-yielding and sustainable, without creating risks for the environment and human health. Therefore, natural products are gaining attention in the production of safe and nutritious food. In a systematic effort to develop affordable and effective biostimulants, we examined the impact of botanical extracts on the growth and physiological parameters of radish plants under field conditions. Ultrasound-assisted extraction, mechanical homogenization, and water were used for the production of potential plant-based biostimulants. Foliar applications of the bio-products, developed and used in our study, have led to an increase in the examined parameters (total yield, dry weight, photosynthetic pigments, vitamin C, nitrates, and micro- and macroelements). A decrease in the total phenolic compounds content was also noted, as well as a varied impact on the steam volatile compounds, fatty acids, sterol, and glucosinolates composition. The most beneficial effects on radish, in terms of physiological and biochemical properties, were found in groups treated with extracts based on the common dandelion, valerian, and giant goldenrod. This innovative approach presented in our study could provide a valuable tool for sustainable horticultural production.

1 April 2021 in Molecules
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KPKatarzyna Pacyga
Katarzyna Pacyga
PPPaweł Wiesław Pacyga
Paweł Wiesław Pacyga
Izabela MichalakIzabela Michalak
Izabela Michalak
Anita BiesiadaAnita Biesiada
Anita Biesiada
Antoni Jacek SzumnyAntoni Jacek Szumny
Antoni Jacek Szumny
UPUrszula Piszcz
Urszula Piszcz
Nutraceuticals and functional foods are gaining more attention amongst consumers interested in nutritious food. The consumption of foodstuffs with a high content of phytochemicals has been proven to provide various health benefits. The application of biostimulants is a potential strategy to fortify cultivated plants with beneficial bioactive compounds. Nevertheless, it has not yet been established whether the proposed higher plants (St. John’s wort, giant goldenrod, common dandelion, red clover, nettle, and valerian) are appropriate for the production of potential bio-products enhancing the nutritional value of white cabbage. Therefore, this research examines the impact of botanical extracts on the growth and nutritional quality of cabbage grown under field conditions. Two extraction methods were used for the production of water-based bio-products, namely: ultrasound-assisted extraction and mechanical homogenisation. Bio-products were applied as foliar sprays to evaluate their impact on total yield, dry weight, photosynthetic pigments, polyphenols, antioxidant activity, vitamin C, nitrates, micro- and macroelements, volatile compounds, fatty acids, sterols, and sugars. Botanical extracts showed different effects on the examined parameters. The best results in terms of physiological and biochemical properties of cabbage were obtained for extracts from common dandelion, valerian, nettle, and giant goldenrod. When enriched with nutrients, vegetables can constitute a valuable component of functional food.

2 March 2022 in Applied Sciences
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DMDamian Maksimowski
Damian Maksimowski
NPNatalia Pachura-Hanusek
Natalia Pachura-Hanusek
Maciej OziembłowskiMaciej Oziembłowski
Maciej Oziembłowski
Agnieszka Nawirska-OlszańskaAgnieszka Nawirska-Olszańska
Agnieszka Nawirska-Olszańska
Antoni Jacek SzumnyAntoni Jacek Szumny
Antoni Jacek Szumny
Due to the dynamic growth of the cold brew coffee market, the aim of this study was to identify and characterize main bioactive and aromatic compounds that may be helpful for quality control during the production of popular beverages. Using headspace solid-phase microextraction and GC-MS and LC-MS analysis, prepared cold brew coffee extracts were investigated and compared with different green bean roasting profiles and varying extraction temperature and time parameters. In terms of quantitative composition, the study showed that cold brew coffees are an exceptional source of chlorogenic acid. Therefore, they may change consumers purchasing decisions on the beverage market and establish a new and natural substitute for controversial energy drinks. The analyses confirm the possibility of producing a beverage with increased chlorogenic acid content above 900 mg/L or at a similar level of 400–500 mg/L with caffeine, which may be important on an industrial scale due to the possibility of diversifying beverage production. Furthermore, aroma compounds were presented as markers responsible for fruity or caramel–roasted-almond notes and changes in their concentrations according to the recipe were also presented. The best option for cold brew coffee production appears to be beans roasted in the 210–220 °C temperature range.