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This study proposes a review on hyaluronic acid (HA) known as hyaluronan or hyaluronate and its derivates and their application in cosmetic formulations. HA is a glycosaminoglycan constituted from two disaccharides (N-acetylglucosamine and D-glucuronic acid), isolated initially from the vitreous humour of the eye, and subsequently discovered in different tissues or fluids (especially in the articular cartilage and the synovial fluid). It is ubiquitous in vertebrates, including humans, and it is involved in diverse biological processes, such as cell differentiation, embryological development, inflammation, wound healing, etc. HA has many qualities that recommend it over other substances used in skin regeneration, with moisturizing and anti-ageing effects. HA molecular weight influences its penetration into the skin and its biological activity. Considering that, nowadays, hyaluronic acid has a wide use and a multitude of applications (in ophthalmology, arthrology, pneumology, rhinology, aesthetic medicine, oncology, nutrition, and cosmetics), the present study describes the main aspects related to its use in cosmetology. The biological effect of HA on the skin level and its potential adverse effects are discussed. Some available cosmetic products containing HA have been identified from the brand portfolio of most known manufacturers and their composition was evaluated. Further, additional biological effects due to the other active ingredients (plant extracts, vitamins, amino acids, peptides, proteins, saccharides, probiotics, etc.) are presented, as well as a description of their possible toxic effects.
Anca Juncan; Dana Moisă; Antonello Santini; Claudiu Morgovan; Luca-Liviu Rus; Andreea Vonica-Țincu; Felicia Loghin. Advantages of Hyaluronic Acid and Its Combination with Other Bioactive Ingredients in Cosmeceuticals. Molecules 2021, 26, 4429 .
AMA StyleAnca Juncan, Dana Moisă, Antonello Santini, Claudiu Morgovan, Luca-Liviu Rus, Andreea Vonica-Țincu, Felicia Loghin. Advantages of Hyaluronic Acid and Its Combination with Other Bioactive Ingredients in Cosmeceuticals. Molecules. 2021; 26 (15):4429.
Chicago/Turabian StyleAnca Juncan; Dana Moisă; Antonello Santini; Claudiu Morgovan; Luca-Liviu Rus; Andreea Vonica-Țincu; Felicia Loghin. 2021. "Advantages of Hyaluronic Acid and Its Combination with Other Bioactive Ingredients in Cosmeceuticals." Molecules 26, no. 15: 4429.
Obesity induces hemodynamic and humoral changes that are associated with functional and structural cardiac remodeling, which ultimately result in the development of heart failure (HF) with preserved ejection fraction (HFpEF). In recent years, pharmacological studies in patients with HFpEF were mostly unsatisfactory. In these conditions, alternative new therapeutic approaches are necessary. The aim of our study was (1) to assess the effects of obesity on heart function in an experimental model and (2) to evaluate the efficacy of an alpha-lipoic acid (ALA) antioxidant treatment. Sprague-Dawley rats (7 weeks old) were either included in the control group (n = 6) or subjected to abdominal aortic banding (AAB) and divided into three subgroups, depending on their diet: standard (AAB + SD, n = 8), hypecaloric (AAB + HD, n = 8) and hypercaloric with discontinuous ALA treatment (AAB + HD + ALA, n = 9). Body weight (BW), glycemia, echocardiography parameters and plasma hydroperoxides were monitored throughout the study. After 36 weeks, plasma adiposity (leptin and adiponectin) and inflammation (IL-6 and TNF-alpha) markers, together with B-type natriuretic peptide and oxidative stress markers (end-products of lipid peroxidation and endogenous antioxidant systems) were assessed. Moreover, cardiac fiber diameters were measured. In our experiment, diet-induced obesity generated cardiometabolic disturbances, and in association with pressure-overload induced by AAB, it precipitated the onset of heart failure, cardiac hypertrophy and diastolic dysfunction, while producing a pro-oxidant and pro-inflammatory plasmatic status. In relationship with its antioxidant effects, the chronic ALA-discontinuous treatment prevented BW gain and decreased metabolic and cardiac perturbations, confirming its protective effects on the cardiovascular system.
Cristina Pop; Maria-Georgia Ștefan; Dana-Maria Muntean; Laurențiu Stoicescu; Adrian Gal; Béla Kiss; Claudiu Morgovan; Felicia Loghin; Luc Rochette; Benjamin Lauzier; Cristina Mogoșan; Steliana Ghibu. Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats. Antioxidants 2020, 9, 1073 .
AMA StyleCristina Pop, Maria-Georgia Ștefan, Dana-Maria Muntean, Laurențiu Stoicescu, Adrian Gal, Béla Kiss, Claudiu Morgovan, Felicia Loghin, Luc Rochette, Benjamin Lauzier, Cristina Mogoșan, Steliana Ghibu. Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats. Antioxidants. 2020; 9 (11):1073.
Chicago/Turabian StyleCristina Pop; Maria-Georgia Ștefan; Dana-Maria Muntean; Laurențiu Stoicescu; Adrian Gal; Béla Kiss; Claudiu Morgovan; Felicia Loghin; Luc Rochette; Benjamin Lauzier; Cristina Mogoșan; Steliana Ghibu. 2020. "Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats." Antioxidants 9, no. 11: 1073.
Antioxidant dietary intervention is considered a potential strategy in delaying age-related dysfunctions. In this study of 56 days, we assessed the antioxidant effects of walnut kernel (WK) and walnut septum extract (WSE) in a D-galactose (D-gal)-induced aging model and in a naturally aged rat model. Young Wistar rats, treated with D-gal (1200 mg/week), and old rats received daily WK or WSE added to the feed. After 8 weeks, blood, liver, and brain samples were collected and hematological, biochemical, oxidative stress biomarkers, histological, and immunohistochemical analyses were performed. Moreover, acetylcholinesterase activity was investigated in brain homogenates. The outcomes demonstrated significant improvement in cellular antioxidant activity and/or decrease of reactive oxygen species, advanced glycation end products, nitric oxide, malondialdehyde, or increase of glutathione after WK or WSE intake in both models. Additionally, WSE showed hypoglycemic effect, and both WK and WSE lowered acetylcholinesterase activity. Both diets could protect neurons against the induced senescence and could reverse the pathological conditions in the physiological aged brain. Thus, dietary supplementation with WK or WSE can maintain the liver and brain health and reduce the risk of age-related diseases, as well as delaying the onset of aging processes.
Marius Emil Rusu; Carmen Georgiu; Anca Pop; Andrei Mocan; Bela Kiss; Oliviu Vostinaru; Ionel Fizesan; Maria-Georgia Stefan; Ana-Maria Gheldiu; Letitia Mates; Rebeca Moldovan; Dana Maria Muntean; Felicia Loghin; Laurian Vlase; Daniela-Saveta Popa. Antioxidant Effects of Walnut (Juglans regia L.) Kernel and Walnut Septum Extract in a D-Galactose-Induced Aging Model and in Naturally Aged Rats. Antioxidants 2020, 9, 424 .
AMA StyleMarius Emil Rusu, Carmen Georgiu, Anca Pop, Andrei Mocan, Bela Kiss, Oliviu Vostinaru, Ionel Fizesan, Maria-Georgia Stefan, Ana-Maria Gheldiu, Letitia Mates, Rebeca Moldovan, Dana Maria Muntean, Felicia Loghin, Laurian Vlase, Daniela-Saveta Popa. Antioxidant Effects of Walnut (Juglans regia L.) Kernel and Walnut Septum Extract in a D-Galactose-Induced Aging Model and in Naturally Aged Rats. Antioxidants. 2020; 9 (5):424.
Chicago/Turabian StyleMarius Emil Rusu; Carmen Georgiu; Anca Pop; Andrei Mocan; Bela Kiss; Oliviu Vostinaru; Ionel Fizesan; Maria-Georgia Stefan; Ana-Maria Gheldiu; Letitia Mates; Rebeca Moldovan; Dana Maria Muntean; Felicia Loghin; Laurian Vlase; Daniela-Saveta Popa. 2020. "Antioxidant Effects of Walnut (Juglans regia L.) Kernel and Walnut Septum Extract in a D-Galactose-Induced Aging Model and in Naturally Aged Rats." Antioxidants 9, no. 5: 424.
We report the synthesis of magnetite nanoparticles (IOMNPs) using the polyol method performed at elevated temperature (300 °C) and high pressure. The ferromagnetic polyhedral IOMNPs exhibited high saturation magnetizations at room temperature (83 emu/g) and a maximum specific absorption rate (SAR) of 2400 W/gFe in water. The uniform dispersion of IOMNPs in solid matrix led to a monotonous increase of SAR maximum (3600 W/gFe) as the concentration decreased. Cytotoxicity studies on two cell lines (cancer and normal) using Alamar Blues and Neutral Red assays revealed insignificant toxicity of the IOMNPs on the cells up to a concentration of 1000 μg/mL. The cells internalized the IOMNPs inside lysosomes in a dose-dependent manner, with higher amounts of IOMNPs in cancer cells. Intracellular hyperthermia experiments revealed a significant increase in the macroscopic temperatures of the IOMNPs loaded cell suspensions, which depend on the amount of internalized IOMNPs and the alternating magnetic field amplitude. The cancer cells were found to be more sensitive to the intracellular hyperthermia compared to the normal ones. For both cell lines, cells heated at the same macroscopic temperature presented lower viability at higher amplitudes of the alternating magnetic field, indicating the occurrence of mechanical or nanoscale heating effects.
Cristian Iacovita; Ionel Fizeșan; Anca Pop; Lavinia Scorus; Roxana Dudric; Gabriela Stiufiuc; Nicoleta Vedeanu; Romulus Tetean; Felicia Loghin; Rares Stiufiuc; Constantin Mihai Lucaciu. In Vitro Intracellular Hyperthermia of Iron Oxide Magnetic Nanoparticles, Synthesized at High Temperature by a Polyol Process. Pharmaceutics 2020, 12, 424 .
AMA StyleCristian Iacovita, Ionel Fizeșan, Anca Pop, Lavinia Scorus, Roxana Dudric, Gabriela Stiufiuc, Nicoleta Vedeanu, Romulus Tetean, Felicia Loghin, Rares Stiufiuc, Constantin Mihai Lucaciu. In Vitro Intracellular Hyperthermia of Iron Oxide Magnetic Nanoparticles, Synthesized at High Temperature by a Polyol Process. Pharmaceutics. 2020; 12 (5):424.
Chicago/Turabian StyleCristian Iacovita; Ionel Fizeșan; Anca Pop; Lavinia Scorus; Roxana Dudric; Gabriela Stiufiuc; Nicoleta Vedeanu; Romulus Tetean; Felicia Loghin; Rares Stiufiuc; Constantin Mihai Lucaciu. 2020. "In Vitro Intracellular Hyperthermia of Iron Oxide Magnetic Nanoparticles, Synthesized at High Temperature by a Polyol Process." Pharmaceutics 12, no. 5: 424.
Despite recent advances in disease management and prevention, heart failure (HF) prevalence is still high. Hypertension, inflammation and oxidative stress are being investigated as important causative processes in HF. L. barbarum L. polysaccharides (LBPs) are widely used for their anti-inflammatory and antioxidant properties. Thus, the aim of the present study was to evaluate the effects of LBPs on inflammation and oxidative stress markers in a pressure overload-induced HF rat model, surgically induced by abdominal aorta banding in Wistar rats (AAB) (n = 28). Also, control rats (n = 10) were subjected to a sham operation. After echocardiographic confirmation of HF (week 24), AAB rats were divided into three groups: rats treated with LBPs for 12 weeks: 100 mg/kg body weight /day (AAB_100, n = 9), 200 mg/kg body weight /day (AAB_200, n = 7) and no-treatment group (control AAB, n = 12). After 12 weeks of treatment with LBPs, the decline of cardiac function was prevented compared to the control AAB rats. Treatment with 200 mg/kg body weight /day LBPs significantly reduced the inflammation as seen by cytokine levels (IL-6 and TNF-α) and the plasma lipid peroxidation, as seen by malondialdehyde levels. These results suggest that LBPs present anti-inflammatory and antioxidant effects with utility in a HF animal model and encourage further investigation of the cardioprotective effects of these polysaccharides.
Cristina Pop; Cristian Berce; Steliana Ghibu; Iuliu Scurtu; Olga Sorițău; Cezar Login; Béla Kiss; Maria Georgia Stefan; Ionel Fizeșan; Horațiu Silaghi; Andrei Mocan; Gianina Crișan; Felicia Loghin; Cristina Mogoșan. Effects of Lycium barbarum L. Polysaccharides on Inflammation and Oxidative Stress Markers in a Pressure Overload-Induced Heart Failure Rat Model. Molecules 2020, 25, 466 .
AMA StyleCristina Pop, Cristian Berce, Steliana Ghibu, Iuliu Scurtu, Olga Sorițău, Cezar Login, Béla Kiss, Maria Georgia Stefan, Ionel Fizeșan, Horațiu Silaghi, Andrei Mocan, Gianina Crișan, Felicia Loghin, Cristina Mogoșan. Effects of Lycium barbarum L. Polysaccharides on Inflammation and Oxidative Stress Markers in a Pressure Overload-Induced Heart Failure Rat Model. Molecules. 2020; 25 (3):466.
Chicago/Turabian StyleCristina Pop; Cristian Berce; Steliana Ghibu; Iuliu Scurtu; Olga Sorițău; Cezar Login; Béla Kiss; Maria Georgia Stefan; Ionel Fizeșan; Horațiu Silaghi; Andrei Mocan; Gianina Crișan; Felicia Loghin; Cristina Mogoșan. 2020. "Effects of Lycium barbarum L. Polysaccharides on Inflammation and Oxidative Stress Markers in a Pressure Overload-Induced Heart Failure Rat Model." Molecules 25, no. 3: 466.
Cristina Barbălată; Alina Porfire; Anca Cherfan; Felicia Loghin; Ioan Tomuță. QbD development of a liposomal co-formulation with Doxorubicin and Simvastatin for an enhanced antiproliferative effect on T47D-KBluc cell line. II. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science 2020, 1 .
AMA StyleCristina Barbălată, Alina Porfire, Anca Cherfan, Felicia Loghin, Ioan Tomuță. QbD development of a liposomal co-formulation with Doxorubicin and Simvastatin for an enhanced antiproliferative effect on T47D-KBluc cell line. II. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science. 2020; ():1.
Chicago/Turabian StyleCristina Barbălată; Alina Porfire; Anca Cherfan; Felicia Loghin; Ioan Tomuță. 2020. "QbD development of a liposomal co-formulation with Doxorubicin and Simvastatin for an enhanced antiproliferative effect on T47D-KBluc cell line." II. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science , no. : 1.
The interactions of nanoparticles with living organisms are driven by an interface called the protein corona. This interface is formed when nanoparticles are introduced in biological milieu and proteins are adsorbed at nanoparticles’ surfaces. Understanding the factors that are responsible for the formation and the composition of the protein corona could reveal mechanistic insights that are involved in the interaction of nanoparticles with biological structures. Multiple studies have qualitatively described the protein corona, but just a few have proposed quantification methods, especially for gold nanoparticles. Using bovine serum albumin conjugated with fluorescein-5-isothiocyanate as a model protein, we developed a fluorescent-based quantification method for gold nanoparticles’ protein coronas. The impact of nanoparticle size and surface chemistry was studied, and our research emphasizes that size and surface chemistry are determinant factors: Bigger nanoparticles and amino-modified surface chemistry are responsible for higher protein adsorption compared to smaller ones and carboxyl- or methoxy-modified surface chemistry. The proposed method can be used to complete the full picture of the interactions of nanoparticles with biological milieu and to describe the parameters which govern these interactions for the better development of nanomedicines.
Raul Nicoară; Maria Ilieș; Alina Uifălean; Cristina Adela Iuga; Felicia Loghin. Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry. Applied Sciences 2019, 9, 4789 .
AMA StyleRaul Nicoară, Maria Ilieș, Alina Uifălean, Cristina Adela Iuga, Felicia Loghin. Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry. Applied Sciences. 2019; 9 (22):4789.
Chicago/Turabian StyleRaul Nicoară; Maria Ilieș; Alina Uifălean; Cristina Adela Iuga; Felicia Loghin. 2019. "Quantification of the PEGylated Gold Nanoparticles Protein Corona. Influence on Nanoparticle Size and Surface Chemistry." Applied Sciences 9, no. 22: 4789.
Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry.
Marius Emil Rusu; Ionel Fizeșan; Anca Pop; Ana-Maria Gheldiu; Andrei Mocan; Gianina Crișan; Laurian Vlase; Felicia Loghin; Daniela-Saveta Popa; Ioan Tomuta; Rusu; Pop; Popa. Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities. Antioxidants 2019, 8, 460 .
AMA StyleMarius Emil Rusu, Ionel Fizeșan, Anca Pop, Ana-Maria Gheldiu, Andrei Mocan, Gianina Crișan, Laurian Vlase, Felicia Loghin, Daniela-Saveta Popa, Ioan Tomuta, Rusu, Pop, Popa. Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities. Antioxidants. 2019; 8 (10):460.
Chicago/Turabian StyleMarius Emil Rusu; Ionel Fizeșan; Anca Pop; Ana-Maria Gheldiu; Andrei Mocan; Gianina Crișan; Laurian Vlase; Felicia Loghin; Daniela-Saveta Popa; Ioan Tomuta; Rusu; Pop; Popa. 2019. "Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities." Antioxidants 8, no. 10: 460.
The present study aimed to evaluate the potential differences in the biological effects of two types of spherical silver particles of 20 and 200 nm (Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of 50 nm and length up to 50 μm, using a complex 3D model representative for the alveolar barrier cultured at air-liquid interface (ALI). The alveolar model was exposed to 0.05, 0.5 and 5 μg/cm2 of test compounds at ALI using a state-of-the-art exposure system (Vitrocell™Cloud System). Endpoints related to the oxidative stress induction, anti-oxidant defence mechanisms, pro-inflammatory responses and cellular death were selected to evaluate the biocompatibility of silver particles and nanowires (AgNMs) and to further ascribe particular biological effects to the different morphologic properties between the three types of AgNMs evaluated. Significant cytotoxic effect was observed for all three types of AgNMs at the highest tested doses. The increased mRNA levels of the pro-apoptotic gene CASP7 suggests that apoptosis may occur after exposure to AgNWs. All three types of AgNMs increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of the metal-binding anti-oxidant metallothioneins (MTs), with AgNWs being the most potent inducer. Even though all types of AgNMs induced the nuclear translocation of NF-kB, only AgNWs increased the mRNA level of pro-inflammatory mediators. The pro-inflammatory response elicited by AgNWs was further confirmed by the increased secretion of the 10 evaluated interleukins. In the current study, we demonstrated that the direct exposure of a complex tetra-culture alveolar model to different types of AgNMs at ALI induces shape- and size-specific biological responses. From the three AgNMs tested, AgNWs were the most potent in inducing biological alterations. Starting from 50 ng/cm2, a dose representative for an acute exposure in a high exposure occupational setting, AgNWs induced prominent changes indicative for a pro-inflammatory response. Even though the acute responses towards a dose representative for a full-lifetime exposure were also evaluated, chronic exposure scenarios at low dose are still unquestionably needed to reveal the human health impact of AgNMs during realistic conditions.
Ionel Fizeșan; Sébastien Cambier; Elisa Moschini; Aline Chary; Inge Nelissen; Johanna Ziebel; Jean-Nicolas Audinot; Tom Wirtz; Marcin Kruszewski; Anca Pop; Béla Kiss; Tommaso Serchi; Felicia Loghin; Arno C. Gutleb. In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires. Particle and Fibre Toxicology 2019, 16, 1 -21.
AMA StyleIonel Fizeșan, Sébastien Cambier, Elisa Moschini, Aline Chary, Inge Nelissen, Johanna Ziebel, Jean-Nicolas Audinot, Tom Wirtz, Marcin Kruszewski, Anca Pop, Béla Kiss, Tommaso Serchi, Felicia Loghin, Arno C. Gutleb. In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires. Particle and Fibre Toxicology. 2019; 16 (1):1-21.
Chicago/Turabian StyleIonel Fizeșan; Sébastien Cambier; Elisa Moschini; Aline Chary; Inge Nelissen; Johanna Ziebel; Jean-Nicolas Audinot; Tom Wirtz; Marcin Kruszewski; Anca Pop; Béla Kiss; Tommaso Serchi; Felicia Loghin; Arno C. Gutleb. 2019. "In vitro exposure of a 3D-tetraculture representative for the alveolar barrier at the air-liquid interface to silver particles and nanowires." Particle and Fibre Toxicology 16, no. 1: 1-21.
To evaluate the influence of weather conditions on mycotoxin presence in wheat, deoxynivalenol (DON), 3-acetyldeoxynivalenol (3AcDON), 15-acetyldeoxynivalenol (15AcDON), fusarenon-X (FUS-X), nivalenol (NIV), HT-2 toxin (HT-2), T-2 toxin (T-2), diacetoxyscirpenol (DAS), neosolaniol (NEO) and zearalenone (ZEN) were evaluated in 102 Romanian wheat samples coming from five wheat growing areas during 2015. Only six mycotoxins were detected, while FUS-X, DAS, NEO and NIV were not present in the wheat samples. Mycotoxin concentrations were correlated with precipitation and temperature values during anthesis and the preharvest period. Overall, the highest frequency was registered for DON, while the lowest frequency was registered for NIV. In the North Muntenia, DON and ZEN registered high frequencies (68% and 16%, respectively). This region was characterized in June and July by medium to high values of rainfall (41–100 mm/month) and normal temperatures (mean of 20.0 °C in June and 24.0 °C in July), suggesting that precipitation levels influence fungi and mycotoxin development to a greater extent compared to the influence of temperature.
Oana Stanciu; Cristina Juan; Houda Berrada; Doina Miere; Felicia Loghin; Jordi Mañes. Study on Trichothecene and Zearalenone Presence in Romanian Wheat Relative to Weather Conditions. Toxins 2019, 11, 163 .
AMA StyleOana Stanciu, Cristina Juan, Houda Berrada, Doina Miere, Felicia Loghin, Jordi Mañes. Study on Trichothecene and Zearalenone Presence in Romanian Wheat Relative to Weather Conditions. Toxins. 2019; 11 (3):163.
Chicago/Turabian StyleOana Stanciu; Cristina Juan; Houda Berrada; Doina Miere; Felicia Loghin; Jordi Mañes. 2019. "Study on Trichothecene and Zearalenone Presence in Romanian Wheat Relative to Weather Conditions." Toxins 11, no. 3: 163.
Efforts for the early diagnosis of the pancreatic ductal adenocarcinoma (PDAC) have recently been driven to one of the precursor lesions, namely intraductal papillary mucinous neoplasm of the pancreas (IPMN). Only a few studies have focused on IPMN molecular biology and its overall progression to cancer. Therefore, IPMN lacks comprehensive characterization which makes its clinical management controversial. In this study, we characterized plasma proteins in the presence of IPMNs in comparison to healthy controls, chronic pancreatitis, and PDAC by a proteomics approach using data-independent acquisition based mass spectrometry. We describe several protein sets that could aid IPMN diagnosis, but also differentiation of IPMN from healthy controls, as well as from benign and malignant diseases. Among all, high levels of carbonic anhydrases and hemoglobins were characteristic for the IPMN group. By employing ELISA based quantification we validated our results for human tissue inhibitor of metalloproteinase inhibitor 1 (TIMP-1). We consider IPMN management directed towards an early potential cancer development a crucial opportunity before PDAC initiation and thus its early detection and cure.
Maria Ilies; Praveen Kumar Sappa; Cristina Adela Iuga; Felicia Loghin; Manuela Gesell Salazar; Frank Ulrich Weiss; Georg Beyer; Markus M. Lerch; Uwe Völker; Julia Mayerle; Elke Hammer. Plasma protein profiling of patients with intraductal papillary mucinous neoplasm of the pancreas as potential precursor lesions of pancreatic cancer. Clinica Chimica Acta 2018, 477, 127 -134.
AMA StyleMaria Ilies, Praveen Kumar Sappa, Cristina Adela Iuga, Felicia Loghin, Manuela Gesell Salazar, Frank Ulrich Weiss, Georg Beyer, Markus M. Lerch, Uwe Völker, Julia Mayerle, Elke Hammer. Plasma protein profiling of patients with intraductal papillary mucinous neoplasm of the pancreas as potential precursor lesions of pancreatic cancer. Clinica Chimica Acta. 2018; 477 ():127-134.
Chicago/Turabian StyleMaria Ilies; Praveen Kumar Sappa; Cristina Adela Iuga; Felicia Loghin; Manuela Gesell Salazar; Frank Ulrich Weiss; Georg Beyer; Markus M. Lerch; Uwe Völker; Julia Mayerle; Elke Hammer. 2018. "Plasma protein profiling of patients with intraductal papillary mucinous neoplasm of the pancreas as potential precursor lesions of pancreatic cancer." Clinica Chimica Acta 477, no. : 127-134.
The results of national safety studies are essential for decision-making at the regulatory level and have also educational implications for prescribing patterns. The aim of the present study was to analyze the ADRs of analgesic medicines spontaneously reported to Pharmacovigilance and Risk Management Service of the Romanian National Agency for Medicines and Medical Devices between 2011 and 2015. For the 71 reports, patients had a mean age of 39.8 years; 60.6% of patients were female, 38% male and 1.4% were unknown. Reporters were mainly physicians (74.7%) and 52.1% of ADR reports were transmitted through marketing authorization holders. Out of the serious ADRs (32.4%), 34.7% led to hospitalization or prolonged hospitalization. The most frequent ADRs reported were skin and subcutaneous tissue disorders (25.8%) and general disorders and administration site conditions (19.2%). Metamizole, alone or in combination, was the main analgesic suspected in almost 15.5% of the cases and remains one of the most popular analgesics in Romania. Ten cases were assessed as preventable or potentially preventable (14%), of which two cases were serious. Even if the level of reporting is still low, this study conducted on ADRs of analgesics reported to the national pharmacovigilance centre represents an essential step towards promoting the rational use of analgesics in Romania. This article is protected by copyright. All rights reserved.
Irina Cazacu; Roxana Stroe; Roxana Dondera; Cristina Mogosan; Francoise Haramburu; Annie Fourrier-Réglat; Felicia Loghin. Adverse drug reactions of analgesic medicines: analysis of the Romanian pharmacovigilance database. Fundamental & Clinical Pharmacology 2018, 32, 330 -336.
AMA StyleIrina Cazacu, Roxana Stroe, Roxana Dondera, Cristina Mogosan, Francoise Haramburu, Annie Fourrier-Réglat, Felicia Loghin. Adverse drug reactions of analgesic medicines: analysis of the Romanian pharmacovigilance database. Fundamental & Clinical Pharmacology. 2018; 32 (3):330-336.
Chicago/Turabian StyleIrina Cazacu; Roxana Stroe; Roxana Dondera; Cristina Mogosan; Francoise Haramburu; Annie Fourrier-Réglat; Felicia Loghin. 2018. "Adverse drug reactions of analgesic medicines: analysis of the Romanian pharmacovigilance database." Fundamental & Clinical Pharmacology 32, no. 3: 330-336.
Irina Cazacu; Annie Fourier-Reglat; Francoise Haramburu; Oliviu Vostinaru; Cristina Mogosan; Felicia Loghin. Pharmaceutical Market of Analgesic Medicines in Romania. Clinical Therapeutics 2017, 39, e5 .
AMA StyleIrina Cazacu, Annie Fourier-Reglat, Francoise Haramburu, Oliviu Vostinaru, Cristina Mogosan, Felicia Loghin. Pharmaceutical Market of Analgesic Medicines in Romania. Clinical Therapeutics. 2017; 39 (8):e5.
Chicago/Turabian StyleIrina Cazacu; Annie Fourier-Reglat; Francoise Haramburu; Oliviu Vostinaru; Cristina Mogosan; Felicia Loghin. 2017. "Pharmaceutical Market of Analgesic Medicines in Romania." Clinical Therapeutics 39, no. 8: e5.
Complete blood protein profiles of 4 different blood sample collection methods (EDTA-, heparin- and citrate plasma and serum) were investigated and the data presented herein is an extension of the research article in Ilies et al. [1]. Specimens were depleted of 6 highly abundant proteins and protein profiling was assessed by nano-LC UDMSE. Exhaustive protein sets and protein abundances before and after depletion are presented in tables and figures. Also, the core protein set and the unique proteins for each sample collection method previously described [1] are disclosed.
Maria Ilies; Cristina Adela Iuga; Felicia Loghin; Vishnu Mukund Dhople; Thomas Thiele; Uwe Völker; Elke Hammer. Data on the impact of the blood sample collection methods on blood protein profiling studies. Data in Brief 2017, 14, 313 -319.
AMA StyleMaria Ilies, Cristina Adela Iuga, Felicia Loghin, Vishnu Mukund Dhople, Thomas Thiele, Uwe Völker, Elke Hammer. Data on the impact of the blood sample collection methods on blood protein profiling studies. Data in Brief. 2017; 14 ():313-319.
Chicago/Turabian StyleMaria Ilies; Cristina Adela Iuga; Felicia Loghin; Vishnu Mukund Dhople; Thomas Thiele; Uwe Völker; Elke Hammer. 2017. "Data on the impact of the blood sample collection methods on blood protein profiling studies." Data in Brief 14, no. : 313-319.
In this study, a total of 244 wheat and wheat-based products collected from Romania were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) in order to evaluate the presence of four enniatins (ENs; i.e., ENA, ENA1, ENB, and ENB1) and beauvericin (BEA). For the wheat samples, the influence of agricultural practices was assessed, whereas the results for the wheat-based products were used to calculate the estimated daily intake of emerging mycotoxins through wheat consumption for the Romanian population. ENB presented the highest incidence (41% in wheat and 32% in wheat-based products), with its maximum levels of 815 μg kg−1 and 170 μg kg−1 in wheat and wheat-based products, respectively. The correlation between the concentrations of ENB and ENB1 in wheat grain samples and farm practices (organic or conventional) was confirmed statistically (p < 0.05). This is the first study that provides comprehensive information about the influence of agricultural practice on emerging Fusarium mycotoxin presence in Romanian wheat samples and the estimated daily intake of ENs and BEA present in wheat-based products for human consumption commercialized in Romania.
Oana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption. Toxins 2017, 9, 189 .
AMA StyleOana Stanciu, Cristina Juan, Doina Miere, Felicia Loghin, Jordi Mañes. Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption. Toxins. 2017; 9 (6):189.
Chicago/Turabian StyleOana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. 2017. "Presence of Enniatins and Beauvericin in Romanian Wheat Samples: From Raw Material to Products for Direct Human Consumption." Toxins 9, no. 6: 189.
Pre-analytical factors have a significant impact on the integrity of blood samples used for qualitative and quantitative protein profiling. Important factors are the type of the blood collection tube and the anticoagulant used. Only a few studies have been performed to assess these variables by comparing only serum and EDTA plasma collection tubes with some target proteins or peptides. In this study, we investigated the protein profile of blood samples collected in serum, EDTA-, heparin-, and citrate plasma tubes. Furthermore, we compared the depletion efficiency of 6 high abundant proteins, the detectable blood protein profile, the variance, and the coverage of the detectable protein sets. The largest differences were found between serum and plasma samples with respect to the peptide number and the occurrence of classical blood proteins. The heparin plasma evidenced a high number of detectable proteins, low global variance and a high similarity to EDTA- and citrate plasma and may therefore be also a useful test tube for blood protein profiling. In addition, a core set of blood proteins were described and the portions and compositions of sampling specific proteins were disclosed. Therefore, pre-analytical issues such as the sample collection method should be considered for protein profiling studies.
Maria Ilies; Cristina Adela Iuga; Felicia Loghin; Vishnu Mukund Dhople; Thomas Thiele; Uwe Völker; Elke Hammer. Impact of blood sample collection methods on blood protein profiling studies. Clinica Chimica Acta 2017, 471, 128 -134.
AMA StyleMaria Ilies, Cristina Adela Iuga, Felicia Loghin, Vishnu Mukund Dhople, Thomas Thiele, Uwe Völker, Elke Hammer. Impact of blood sample collection methods on blood protein profiling studies. Clinica Chimica Acta. 2017; 471 ():128-134.
Chicago/Turabian StyleMaria Ilies; Cristina Adela Iuga; Felicia Loghin; Vishnu Mukund Dhople; Thomas Thiele; Uwe Völker; Elke Hammer. 2017. "Impact of blood sample collection methods on blood protein profiling studies." Clinica Chimica Acta 471, no. : 128-134.
In this study, the presence of fourteen Fusarium mycotoxins, legislated by the European Union – deoxynivalenol, zearalenone, HT-2 and T-2 toxins (EC/1881/2006; 2013/165/EU), or non-legislated (five trichothecens and five "emerging" mycotoxins), was evaluated in 31 whole unprocessed wheat samples and 35 white wheat flour samples from different areas of Romania. For this purpose, a validated multi-mycotoxins liquid chromatography tandem mass spectrometry method was applied. Seventy three percent of the analyzed samples contained at least one mycotoxin. The highest occurrence was for enniatin B, 71% of the analyzed samples being positive (21–407 μg kg−1). Regarding the legislated mycotoxins, deoxynivalenol was detected in 14% (111–1787 μg kg−1) of the samples, while zearalenone was detected in 9% (51–1135 μg kg−1). Only one sample was positive for neosolaniol. Concerning the co-occurrence, 42% of the samples were contaminated with two to five mycotoxins, the most frequent being the binary or tertiary combinations of enniatins. This is the first study applied to Romanian wheat grains and flour samples using a high sensitive multi-mycotoxins method, and which included also "emerging" mycotoxins.
Oana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. Occurrence and co-occurrence of Fusarium mycotoxins in wheat grains and wheat flour from Romania. Food Control 2017, 73, 147 -155.
AMA StyleOana Stanciu, Cristina Juan, Doina Miere, Felicia Loghin, Jordi Mañes. Occurrence and co-occurrence of Fusarium mycotoxins in wheat grains and wheat flour from Romania. Food Control. 2017; 73 ():147-155.
Chicago/Turabian StyleOana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. 2017. "Occurrence and co-occurrence of Fusarium mycotoxins in wheat grains and wheat flour from Romania." Food Control 73, no. : 147-155.
Due to the matrix complexity for wheat-based products, a comparative study of different rapid extraction procedures was performed for the extraction of enniatins (ENA, ENA1, ENB, ENB1) and beauvericin in flour, pasta, breakfast cereals, and biscuits. Three different approaches were studied during the extraction and purification steps (shaker, Ultra-Turrax, and QuEChERS) for each matrix. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray source working in a positive mode was used. For the analysis of the five mycotoxins, the three methods were tested in terms of recovery, matrix effect, and sensibility, concluding that Ultra-Turrax extraction was the most competent method. The applicability of the validated method was demonstrated by analyzing 16 commercial samples from Romania.
Oana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products. CyTA - Journal of Food 2017, 15, 433 -440.
AMA StyleOana Stanciu, Cristina Juan, Doina Miere, Felicia Loghin, Jordi Mañes. Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products. CyTA - Journal of Food. 2017; 15 (3):433-440.
Chicago/Turabian StyleOana Stanciu; Cristina Juan; Doina Miere; Felicia Loghin; Jordi Mañes. 2017. "Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products." CyTA - Journal of Food 15, no. 3: 433-440.
Recently, a series of thiazolo arene ruthenium complexes were found to be highly cytotoxic in vitro, on both cisplatin-sensitive and cisplatin-resistant ovarian cancer cells. The most active compound of the series, [(η6-p-cymene)Ru(L)Cl]Cl (L = 1-(2-(2-(3-chlorobenzylidene)hydrazinyl)-4-methylthiazol-5-yl)ethanone), was selected for an in vivo study in order to assess its safety profile. The ruthenium complex was administered to female Crl:WI rats orally, by gastric intubation and intraperitoneal injection. The hematological parameters and the histopathological changes in liver, kidneys, spleen and brain were investigated after a 14-days treatment. The substance was very well tolerated orally, with a LD50 value of over 2000 mg/kg body weight. Symptoms were observed only in the first day after intraperitoneal administration of the highest dose, with a LD50 value between 300 and 2000 mg/kg bw. The hematological profile was not modified at any of the tested doses, after both oral and intraperitoneal acute administration. Structural modifications (moderate lymphocytolysis) were identified only in the spleen at the highest tested dose. In conclusion, the thiazolo arene ruthenium complex was very well tolerated orally and had a low acute toxicity after intraperitoneal administration in Crl:WI rats The results justify further investigation to determine the in vivo therapeutic potential of this promising ruthenium complex.
Adriana Grozav; Viorel Miclaus; Oliviu Vostinaru; Steliana Ghibu; Cristian Berce; Ioana Rotar; Cristina Mogosan; Bruno Therrien; Felicia Loghin; Daniela-Saveta Popa. Acute toxicity evaluation of a thiazolo arene ruthenium (II) complex in rats. Regulatory Toxicology and Pharmacology 2016, 80, 233 -240.
AMA StyleAdriana Grozav, Viorel Miclaus, Oliviu Vostinaru, Steliana Ghibu, Cristian Berce, Ioana Rotar, Cristina Mogosan, Bruno Therrien, Felicia Loghin, Daniela-Saveta Popa. Acute toxicity evaluation of a thiazolo arene ruthenium (II) complex in rats. Regulatory Toxicology and Pharmacology. 2016; 80 ():233-240.
Chicago/Turabian StyleAdriana Grozav; Viorel Miclaus; Oliviu Vostinaru; Steliana Ghibu; Cristian Berce; Ioana Rotar; Cristina Mogosan; Bruno Therrien; Felicia Loghin; Daniela-Saveta Popa. 2016. "Acute toxicity evaluation of a thiazolo arene ruthenium (II) complex in rats." Regulatory Toxicology and Pharmacology 80, no. : 233-240.
Highlights•Certain food additives & cosmetic preservatives are potential endocrine disruptors.•The selected compounds had no in vitro androgenic activity.•Three of the selected compounds showed in vitro anti-androgenic activity.•The effect of binary mixtures could be predicted by the dose addition model. AbstractThe individual and combined (binary mixtures) (anti)androgenic effect of butylparaben (BuPB), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and propyl gallate (PG) was evaluated using the MDA-kb2 cell line. Exposing these cells to AR agonists results in the expression of the reporter gene (encoding for luciferase) and luminescence can be measured in order to monitor the activity of the reporter protein. In case of the evaluation of the anti-androgenic effect, the individual test compounds or binary mixtures were tested in the presence of a fixed concentration of a strong AR agonist (1000 pM 5-alpha-dihydrotestosterone; DHT). Cell viability was assessed using a resazurin based assay. For PG, this is the first report in the literature concerning its (anti)androgenic activity. In case of both individual and mixture testing none of the compounds or binary combinations showed androgenic activity. When tested in the presence of DHT, BuPB, BHA and BHT proved to be weak anti-androgens and this was confirmed during the evaluation of binary mixtures (BuPB + BHA, BuPB + BHT and BHA + BHT). Besides performing the in vitro testing of the binary combinations, two mathematical models (dose addition and response addition) were evaluated in terms of accuracy of prediction of the anti-androgenic effect of the selected binary mixtures. The dose addition model guaranteed a good correlation between the experimental and predicted data. However, no estimation was possible in case of mixtures containing PG, due to the lack of effect of the compound in case of the individual testing.
Anca Pop; Tudor Catalin Drugan; Arno C. Gutleb; Diana Lupu; Julien Cherfan; Felicia Loghin; Béla Kiss. Individual and combined in vitro (anti)androgenic effects of certain food additives and cosmetic preservatives. Toxicology in Vitro 2016, 32, 269 -277.
AMA StyleAnca Pop, Tudor Catalin Drugan, Arno C. Gutleb, Diana Lupu, Julien Cherfan, Felicia Loghin, Béla Kiss. Individual and combined in vitro (anti)androgenic effects of certain food additives and cosmetic preservatives. Toxicology in Vitro. 2016; 32 ():269-277.
Chicago/Turabian StyleAnca Pop; Tudor Catalin Drugan; Arno C. Gutleb; Diana Lupu; Julien Cherfan; Felicia Loghin; Béla Kiss. 2016. "Individual and combined in vitro (anti)androgenic effects of certain food additives and cosmetic preservatives." Toxicology in Vitro 32, no. : 269-277.