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Total mercury (THg), methylmercury (MeHg), and selenium (Se) concentrations were measured in various commercially important fish species. The benefit–risk binomial associated with these chemicals was assessed in children through the probability of exceeding the provisional tolerable weekly intakes (PTWIs) of the contaminants and the Se recommended dietary allowance (RDA). The Se:Hg molar ratios, selenium health benefit values (HBVSe), and monthly consumption rate limits (CRmm) for each species were also calculated. THg and Se were analyzed by atomic absorption spectrophotometer (Shimadzu, Milan, Italy), while MeHg was determined by Trace Ultra gas chromatograph connected with a PolarisQ MS (Thermo Fisher Scientific, Waltham, MA, USA). None of the analyzed fish had Hg levels above the European Community regulatory limits, while most large predators had MeHg levels over the threshold concentration set by US EPA. The estimated weekly intakes of THg and MeHg exceeded in many cases the PTWIs and the Se estimated daily intakes were provided from 0.71% to 2.75% of the RDA. Se:Hg molar ratios above 1 and positive HBVSe index suggested that Se in fish could be enough to alleviate the potential toxic effect of Hg. However, high-risk groups as children should consume fish in moderation because a large consumption pattern, especially of swordfish and tunas, might be of concern for health.
Grazia Barone; Arianna Storelli; Daniela Meleleo; Angela Dambrosio; Rita Garofalo; Antonio Busco; Maria Storelli. Levels of Mercury, Methylmercury and Selenium in Fish: Insights into Children Food Safety. Toxics 2021, 9, 39 .
AMA StyleGrazia Barone, Arianna Storelli, Daniela Meleleo, Angela Dambrosio, Rita Garofalo, Antonio Busco, Maria Storelli. Levels of Mercury, Methylmercury and Selenium in Fish: Insights into Children Food Safety. Toxics. 2021; 9 (2):39.
Chicago/Turabian StyleGrazia Barone; Arianna Storelli; Daniela Meleleo; Angela Dambrosio; Rita Garofalo; Antonio Busco; Maria Storelli. 2021. "Levels of Mercury, Methylmercury and Selenium in Fish: Insights into Children Food Safety." Toxics 9, no. 2: 39.
Several studies report the presence of Helicobacter pylori (H. pylori) in seawater either free or attached to planktonic organism. After considering the role played by plankton in the food chain of most aquatic ecosystems and the possible role that seafood products can assume in the transmission of H. pylori to humans, the aim of this study was to assess the survival of H. pylori in artificially contaminated Mytilus galloprovincialis (M. galloprovincialis). A traditional culture method and a reverse transcriptase-PCR (RT-PCR) assay were employed to detect the mRNA of known virulence factor (VacA) which can be considered use a marker of bacterial viability. The obtained results clearly show that H. pylori is able to survive in artificially contaminated mussels for 6 days (2 days in a cultivable form and 4 days in a non-cultivable form).
Nicoletta C. Quaglia; Maria M. Storelli; Teresa Scardocchia; Anna Lattanzi; Gaetano V. Celano; Rosa Monno; Angela Dambrosio. Helicobacter pylori: Survival in cultivable and non-cultivable form in artificially contaminated Mytilus galloprovincialis. International Journal of Food Microbiology 2019, 312, 108363 .
AMA StyleNicoletta C. Quaglia, Maria M. Storelli, Teresa Scardocchia, Anna Lattanzi, Gaetano V. Celano, Rosa Monno, Angela Dambrosio. Helicobacter pylori: Survival in cultivable and non-cultivable form in artificially contaminated Mytilus galloprovincialis. International Journal of Food Microbiology. 2019; 312 ():108363.
Chicago/Turabian StyleNicoletta C. Quaglia; Maria M. Storelli; Teresa Scardocchia; Anna Lattanzi; Gaetano V. Celano; Rosa Monno; Angela Dambrosio. 2019. "Helicobacter pylori: Survival in cultivable and non-cultivable form in artificially contaminated Mytilus galloprovincialis." International Journal of Food Microbiology 312, no. : 108363.
Nicoletta C. Quaglia; Mariamaddalena Storelli; Federica Ioanna; Giuseppe Celano; Gaetano V. Celano; Chiara Conversano; Michele De Rosa; Angela Dambrosio. Listeria monocytogenesand enterotoxigenicStaphylococcus aureusin dry fermented sausages belonging to “Traditional Agri‐Food Product” produced in Southern Italy. Journal of Food Safety 2019, 39, 1 .
AMA StyleNicoletta C. Quaglia, Mariamaddalena Storelli, Federica Ioanna, Giuseppe Celano, Gaetano V. Celano, Chiara Conversano, Michele De Rosa, Angela Dambrosio. Listeria monocytogenesand enterotoxigenicStaphylococcus aureusin dry fermented sausages belonging to “Traditional Agri‐Food Product” produced in Southern Italy. Journal of Food Safety. 2019; 39 (6):1.
Chicago/Turabian StyleNicoletta C. Quaglia; Mariamaddalena Storelli; Federica Ioanna; Giuseppe Celano; Gaetano V. Celano; Chiara Conversano; Michele De Rosa; Angela Dambrosio. 2019. "Listeria monocytogenesand enterotoxigenicStaphylococcus aureusin dry fermented sausages belonging to “Traditional Agri‐Food Product” produced in Southern Italy." Journal of Food Safety 39, no. 6: 1.
The aim of this study was to assess the microbiological quality and safety of some cheeses produced in Apulia region, Southern Italy, and classified as “traditional agri‐food products.” A total of 120 samples were collected and analyzed. From results, 82.5% of cheeses analyzed, had a satisfactory microbiological quality according to the European legislation. The coagulase positive Staphylococci (CPS) count was above the limits set by the European legislation in 17.5% of the samples analyzed. Within the 20 strains of Staphylococcus aureus (S. aureus) isolated, 12 were positive for the detection of the enterotoxin genes. Furthermore, nine strains of S. aureus were positive to reverse passive latex agglutination (SET‐RPLA) assay. One of the two samples of Cacioricotta di Vacca Fresco, with a CPS load of 3.02 × 105 cfu/g, showed unsatisfactory microbiological safety because was positive to SET‐RPLA. Based on the findings, it is essential to apply and maintain good hygiene practices throughout the food chain, especially for traditional agri‐food products, which are often produced in small family processing plants using production protocols based on the experience handed down from father to son. Practical application The traditional agri‐food products are foodstuffs much appreciated by consumers because of their organoleptic qualities and taste that brings back to the genuine flavors of the past. However, the hygienic quality and safety of these products is linked to the personal experience of food business operator and not to the regular checking of microbiological parameters that validate the acceptability of foodstuffs and their manufacturing, handling and distribution processes. The results of this study can contribute to the risk analysis for these particular products, and underline the need to apply and maintain good hygiene practices throughout the production chain.
Angela Dambrosio; Federica Ioanna; Maria M. Storelli; Domenico Castiglia; Francesco Giannico; Maria Antonietta Colonna; Michele De Rosa; Nicoletta C. Quaglia. Microbiological quality and safety of cheeses belonging to “Traditional Agri‐Food Products” (T.A.P.) produced in Southern Italy. Journal of Food Safety 2018, 38, 1 .
AMA StyleAngela Dambrosio, Federica Ioanna, Maria M. Storelli, Domenico Castiglia, Francesco Giannico, Maria Antonietta Colonna, Michele De Rosa, Nicoletta C. Quaglia. Microbiological quality and safety of cheeses belonging to “Traditional Agri‐Food Products” (T.A.P.) produced in Southern Italy. Journal of Food Safety. 2018; 38 (6):1.
Chicago/Turabian StyleAngela Dambrosio; Federica Ioanna; Maria M. Storelli; Domenico Castiglia; Francesco Giannico; Maria Antonietta Colonna; Michele De Rosa; Nicoletta C. Quaglia. 2018. "Microbiological quality and safety of cheeses belonging to “Traditional Agri‐Food Products” (T.A.P.) produced in Southern Italy." Journal of Food Safety 38, no. 6: 1.
Trace element (Hg, Pb, Cd, Zn, Cu, Ni, and Cr) occurrence was determined in the muscle tissue of swordfish collected in the Mediterranean Sea to assess whether the intakes complied with the recommended levels for essential metals and permissible levels for toxic elements. Metals were analyzed by an atomic absorption spectrophotometer (Shimadzu AA 7000). The methodology of Target Hazard Quotient (THQ) was also evaluated. The ranking order of toxic metal concentration was Hg > Cd > Pb, while for essential elements the distribution pattern followed the sequence Zn > Cu > Ni > Cr. The Estimated Weekly Intakes (EWI) as well as THQ for Cd and Pb indicated that swordfish consumption did not pose a risk to human health, whereas the major concern was for Hg. Fish size-related changes in Hg concentrations resulted in high EWI and THQ values relative to larger fish consumption, implying a potential risk to human health. For consumer protection, catches of swordfish approximately above 44 kg should be avoided as these fish have a higher risk of containing toxic levels of Hg.
Grazia Barone; Angela Dambrosio; Arianna Storelli; Rita Garofalo; Vito Pietro Busco; Maria Maddalena Storelli. Estimated Dietary Intake of Trace Metals from Swordfish Consumption: A Human Health Problem. Toxics 2018, 6, 22 .
AMA StyleGrazia Barone, Angela Dambrosio, Arianna Storelli, Rita Garofalo, Vito Pietro Busco, Maria Maddalena Storelli. Estimated Dietary Intake of Trace Metals from Swordfish Consumption: A Human Health Problem. Toxics. 2018; 6 (2):22.
Chicago/Turabian StyleGrazia Barone; Angela Dambrosio; Arianna Storelli; Rita Garofalo; Vito Pietro Busco; Maria Maddalena Storelli. 2018. "Estimated Dietary Intake of Trace Metals from Swordfish Consumption: A Human Health Problem." Toxics 6, no. 2: 22.
The aim of this study was to assess the growth and survival of Escherichia coli O157:H7 during the manufacturing and ripening of Cacioricotta goat cheese. Goat milk was artificially contaminated with E. coli O157:H7 and the bacterial load was monitored from production up to 90 days of ripening. Goat milk was inoculated with 102 cfu ml-1 of E. coli O157:H7 and the bacterial count of the curd at time zero was 2.31 log10 cfu g-1. During the first day of ripening, the bacterial load has increased to 5.73 log10 cfu g-1 to more than 6.20 log10 cfu g-1 during the first week. The bacterial load remained constant up to 28 days and then slightly decreased until the end of ripening, with values of aw and pH of 0.88 and 5.41 respectively. The results of this study highlighted that E. coli O157:H7 is able to survive the manufacturing process and they suggest that the 90-day period of ripening alone is insufficient to remove E. coli O157:H7 in contaminated Cacioricotta goat cheese. Moreover, these results support the assumption that the presence of a low contamination of milk with E. coli O157:H7 could represent a potential source of infection and a threat to consumers.
F. Ioanna; Nicoletta Cristiana Quaglia; M.M. Storelli; D. Castiglia; E. Goffredo; A. Storelli; M. De Rosa; G. Normanno; A. Caputi Jambrenghi; A. Dambrosio. Survival of Escherichia coli O157:H7 during the manufacture and ripening of Cacioricotta goat cheese. Food Microbiology 2018, 70, 200 -205.
AMA StyleF. Ioanna, Nicoletta Cristiana Quaglia, M.M. Storelli, D. Castiglia, E. Goffredo, A. Storelli, M. De Rosa, G. Normanno, A. Caputi Jambrenghi, A. Dambrosio. Survival of Escherichia coli O157:H7 during the manufacture and ripening of Cacioricotta goat cheese. Food Microbiology. 2018; 70 ():200-205.
Chicago/Turabian StyleF. Ioanna; Nicoletta Cristiana Quaglia; M.M. Storelli; D. Castiglia; E. Goffredo; A. Storelli; M. De Rosa; G. Normanno; A. Caputi Jambrenghi; A. Dambrosio. 2018. "Survival of Escherichia coli O157:H7 during the manufacture and ripening of Cacioricotta goat cheese." Food Microbiology 70, no. : 200-205.
Grazia Barone; Angela Dambrosio; Arianna Storelli; Antonio Busco; Federica Ioanna; Nicoletta Cristiana Quaglia; Roberto Giacominelli-Stuffler; Maria Maddalena Storelli. Traditional Italian cheeses: Trace element levels and estimation of dietary intake. Journal of Food Composition and Analysis 2018, 66, 205 -211.
AMA StyleGrazia Barone, Angela Dambrosio, Arianna Storelli, Antonio Busco, Federica Ioanna, Nicoletta Cristiana Quaglia, Roberto Giacominelli-Stuffler, Maria Maddalena Storelli. Traditional Italian cheeses: Trace element levels and estimation of dietary intake. Journal of Food Composition and Analysis. 2018; 66 ():205-211.
Chicago/Turabian StyleGrazia Barone; Angela Dambrosio; Arianna Storelli; Antonio Busco; Federica Ioanna; Nicoletta Cristiana Quaglia; Roberto Giacominelli-Stuffler; Maria Maddalena Storelli. 2018. "Traditional Italian cheeses: Trace element levels and estimation of dietary intake." Journal of Food Composition and Analysis 66, no. : 205-211.
Pangasius hypophthalmus fillets, classified according to different frozen technologies, were subjected to microbiological (Psychrophilic Total Aerobic Count = PTAC, Enterobacteriacea, Staphylococcus aureus, Salmonella spp. and Listeria monocytogenes) and chemical analyses (Hg, Pb, Cd and six “indicator” congeners of polychlorinated biphenyls=PCB6). Mean count of psychrophilic aerobic total bacteria, Enterobacteriaceae and S. aureus in all samples examined was 4.44 log CFU/g, 2.16 log CFU/g and 1.07 log CFU/g, respectively. Any sample showed the presence of Salmonella, while L. monocytogenes was encountered in 2.1% of samples tested. The microbiological counts were different among fillet categories, but with values always lower than the limit of ICMSF. Also the chemical quality of processed products is satisfactory being the concentrations of all metals and six “indicator” PCBs below safety levels for human consumption. This study has contributed to addressing the current paucity in literature on the microbial and chemical status of frozen P. hypophthalmus fillets. Practical Applications: The present research reveals a good chemical and microbiological quality of the frozen Pangasius fillets, although the samples examined show a low incidence of Listeria monocytogenes and Staphylococcus aureus. Thus, it would be desirable a greater attention to the operator's personal hygiene practices during the processing procedures, appropriate preservation techniques and sanitation programs in the processing plants to avoid cross-contamination. The data contained in this study may also be useful for national and international food safety regulatory authorities
Angela Dambrosio; Giovanni Normanno; Arianna Storelli; Grazia Barone; Federica Ioanna; Luigi Errico; Gerardo Centoducati; Maria M. Storelli. Aspects of Vietnamese Sutchi Catfish (Pangasius Hypophthalmus ) Frozen Fillet Quality: Microbiological Profile and Chemical Residues. Journal of Food Safety 2016, 36, 532 -536.
AMA StyleAngela Dambrosio, Giovanni Normanno, Arianna Storelli, Grazia Barone, Federica Ioanna, Luigi Errico, Gerardo Centoducati, Maria M. Storelli. Aspects of Vietnamese Sutchi Catfish (Pangasius Hypophthalmus ) Frozen Fillet Quality: Microbiological Profile and Chemical Residues. Journal of Food Safety. 2016; 36 (4):532-536.
Chicago/Turabian StyleAngela Dambrosio; Giovanni Normanno; Arianna Storelli; Grazia Barone; Federica Ioanna; Luigi Errico; Gerardo Centoducati; Maria M. Storelli. 2016. "Aspects of Vietnamese Sutchi Catfish (Pangasius Hypophthalmus ) Frozen Fillet Quality: Microbiological Profile and Chemical Residues." Journal of Food Safety 36, no. 4: 532-536.
PCBs including “indicator” and “dioxin-like” congeners were measured in a marine gastropod, Hexaplex trunculus, from Mediterranean areas exposed to anthropogenic pressure, to evaluate their compliance with European Union (EU) legislation. Total PCB concentrations showed location-dependent variation, and were higher in samples from Ionian Sea-Taranto (1001 ng g−1 lipid weight) compared with those from Adriatic Sea-Bari (519 ng g−1 lipid weight) and Adriatic-Sea-Brindisi (253 ng g−1 lipid weight). All sample concentrations were compliant with EU regulation limits for the sum of six “indicator” PCBs (Ionian Sea-Taranto: 40.7 ng g−1 wet weight; Adriatic Sea-Bari: 24.0 ng g−1 wet weight; Adriatic Sea-Brindisi: 11.0 ng g−1 wet weight). Also “dioxin-like” PCBs content expressed as toxic equivalent (TEQ) values, in samples from Adriatic Sea (Adriatic Sea-Bari: 2.48 pg WHO-TEQ/g wet weight; Adriatic Sea-Brindisi: 2.14 pg WHO-TEQ/g wet weight), were below the limit, while the specimens from the Ionian Sea (Ionian Sea-Taranto: 3.53 pg WHO-TEQ/g wet weight) showed levels exceeding the maximum threshold
Maria Maddalena Storelli; Angela Dambrosio; Arianna Storelli; Grazia Barone; Federica Ioanna; Giovanni Normanno. Levels of polychlorinated biphenyls (PCBs) in marine gastropod Hexaplex trunculus: Compliance with European Union legislation. Journal of Food Composition and Analysis 2014, 36, 35 -39.
AMA StyleMaria Maddalena Storelli, Angela Dambrosio, Arianna Storelli, Grazia Barone, Federica Ioanna, Giovanni Normanno. Levels of polychlorinated biphenyls (PCBs) in marine gastropod Hexaplex trunculus: Compliance with European Union legislation. Journal of Food Composition and Analysis. 2014; 36 (1-2):35-39.
Chicago/Turabian StyleMaria Maddalena Storelli; Angela Dambrosio; Arianna Storelli; Grazia Barone; Federica Ioanna; Giovanni Normanno. 2014. "Levels of polychlorinated biphenyls (PCBs) in marine gastropod Hexaplex trunculus: Compliance with European Union legislation." Journal of Food Composition and Analysis 36, no. 1-2: 35-39.
Thirty-five strains of lactic acid bacteria were isolated from artisanal raw milk cheese, presumptively identified and tested against one dairy Escherichia coli strain. Six lactobacilli, exhibiting antagonistic activity, were identified at the species level and their action was evaluated against four strains of Gram-foodborne pathogens (Escherichia coli O26, Escherichia coli O157:H7, Salmonella spp. 1023, and Salmonella Typhimurium) and the control strain Escherichia coli ATCC 45922. The antagonistic activity was determined by spot method and the inhibition zones were measured by Autodesk AutoCAD 2007. Three strains, all Lactobacillus paracasei, were active against all the pathogens; the other strains, all Lactobacillus plantarum, showed antagonistic activity against some pathogens. This study highlights the intense and different antagonistic activity induced by lactobacilli against various foodborne pathogens thus demonstrating that using selected lactic acid bacteria strains as adjunct cultures could be an effective strategy to prevent the development of foodborne pathogens in artisanal raw milk cheeses, and thus improving their safety.
Marco Geria; Angela Dambrosio; Giovanni Normanno; Vanessa Lorusso; Andrea Caridi. Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776. Acta Scientiarum. Technology 2013, 36, 1-6 .
AMA StyleMarco Geria, Angela Dambrosio, Giovanni Normanno, Vanessa Lorusso, Andrea Caridi. Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776. Acta Scientiarum. Technology. 2013; 36 (1):1-6.
Chicago/Turabian StyleMarco Geria; Angela Dambrosio; Giovanni Normanno; Vanessa Lorusso; Andrea Caridi. 2013. "Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776." Acta Scientiarum. Technology 36, no. 1: 1-6.
Burrata cheese is a popular typical Italian food product, produced in Puglia (an administrative region of southern Italy), and this study investigated the microbiological quality of 404 samples of this cheese. The samples were analyzed in order to quantify Escherichia coli and to detect the presence of Staphylococcus aureus, Salmonella spp., and Listeria monocytogenes. No sample exceeded the values of E. coli set by EC Regulation 1441/07 for some dairy products, while 15 (3.7%) samples tested coagulase-positive staphylococci positive, with values greater than 103 CFU/g. One strain of S. aureus was identified and characterized from each of these positive samples, and of these strains, 7 (46.6%) produced staphylococcal enterotoxin A, 5 (33.3%) produced staphylococcal enterotoxin C, 2 (13.3%) produced staphylococcal enterotoxin D, and 1 (6.6%) produced both staphylococcal enterotoxins A and D. All strains were mecA negative. The 15 S. aureus isolates were tested for their antimicrobial resistance patterns, and all analyzed strains showed antimicrobial resistance properties for at least one of the tested antibiotics. Testing for the other pathogens mentioned above gave negative results. The results of our study mean that the microbiological quality of Burrata cheese can be assumed to be good, although care must be taken with raw materials and good hygiene during processing in order to guarantee greater food safety.
Angela Dambrosio; Nicoletta Cristiana Quaglia; Mara Saracino; Maria Malcangi; Cosimo Montagna; Marcello Quinto; Vanessa Lorusso; Giovanni Normanno. Microbiological Quality of Burrata Cheese Produced in Puglia Region: Southern Italy. Journal of Food Protection 2013, 76, 1981 -1984.
AMA StyleAngela Dambrosio, Nicoletta Cristiana Quaglia, Mara Saracino, Maria Malcangi, Cosimo Montagna, Marcello Quinto, Vanessa Lorusso, Giovanni Normanno. Microbiological Quality of Burrata Cheese Produced in Puglia Region: Southern Italy. Journal of Food Protection. 2013; 76 (11):1981-1984.
Chicago/Turabian StyleAngela Dambrosio; Nicoletta Cristiana Quaglia; Mara Saracino; Maria Malcangi; Cosimo Montagna; Marcello Quinto; Vanessa Lorusso; Giovanni Normanno. 2013. "Microbiological Quality of Burrata Cheese Produced in Puglia Region: Southern Italy." Journal of Food Protection 76, no. 11: 1981-1984.
Marco Geria; Angela Dambrosio; Giovanni Normanno; Vanessa Lorusso; Andrea Caridi. Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776. Acta Scientiarum. Technology 2013, 36, 1 .
AMA StyleMarco Geria, Angela Dambrosio, Giovanni Normanno, Vanessa Lorusso, Andrea Caridi. Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776. Acta Scientiarum. Technology. 2013; 36 (1):1.
Chicago/Turabian StyleMarco Geria; Angela Dambrosio; Giovanni Normanno; Vanessa Lorusso; Andrea Caridi. 2013. "Antagonistic activity of dairy lactobacilli against gram-foodborne pathogens - doi: 10.4025/actascitechnol.v36i1.18776." Acta Scientiarum. Technology 36, no. 1: 1.
This study examined the effect of different crude protein (CP) levels in the diet on growth performance of broilers as well as the total aerobic mesophilic count (TAMC) and Escherichia coli count (ECC) isolated from chicken faeces. A total 150 day-old broilers (Hubbard) were allocated to three treatments with five replicates containing 10 birds. The dietary treatments consisted of three diets with different CP levels for 42 days: high-protein (HCP, 22.5%), medium-protein (MCP, 20.5%), and low-protein (LCP, 18.5%). Body weight and feed intake were determined and the feed conversion ratio was calculated. Faecal samples were collected at 14, 21 and 42 days. Dietary CP did not affect the growth performance of broilers. Reducing CP level was effective at beneficially modulating the composition of the faecal microflora, in particular TAMC and ECC concentrations (log10 CFU g−1) were significant lower for the LCP birds. During hot temperature conditions (33°C and 70% relative humidity) a low protein diet may help to control the characteristics of the faecal microbial community without negative effects on broiler performance.
Vito Laudadio; Angela Dambrosio; Giovanni Normanno; Rifat Ullah Khan; Shabana Naz; Ebrahim Rowghani; Vincenzo Tufarelli. Effect of Reducing Dietary Protein Level on Performance Responses and some Microbiological Aspects of Broiler Chickens under Summer Environmental Conditions. Avian Biology Research 2012, 5, 88 -92.
AMA StyleVito Laudadio, Angela Dambrosio, Giovanni Normanno, Rifat Ullah Khan, Shabana Naz, Ebrahim Rowghani, Vincenzo Tufarelli. Effect of Reducing Dietary Protein Level on Performance Responses and some Microbiological Aspects of Broiler Chickens under Summer Environmental Conditions. Avian Biology Research. 2012; 5 (2):88-92.
Chicago/Turabian StyleVito Laudadio; Angela Dambrosio; Giovanni Normanno; Rifat Ullah Khan; Shabana Naz; Ebrahim Rowghani; Vincenzo Tufarelli. 2012. "Effect of Reducing Dietary Protein Level on Performance Responses and some Microbiological Aspects of Broiler Chickens under Summer Environmental Conditions." Avian Biology Research 5, no. 2: 88-92.
Mercury, cadmium, and lead concentrations were determined in various fishery products (fishes, cephalopod molluscs, and crustaceans) imported into Italy from .many European and non-European coastal countries. Considerable differences were found in the concentrations of these metals among the products tested. The highest mean Hg concentration was found in fishes (0.21 μg g−1 wet weight), whereas cephalopods had the highest mean Cd concentration (0.35 μg g−1 wet weight). Swordfish (0.80 μg g−1 wet weight), longtail tuna (0.53 μg g−1 wet weight), and thornback ray (0.52 μg g−1 wet weight) had the highest concentrations of Hg, whereas maximum Cd concentrations were found in samples of common cuttlefish (0.85 μg g−1 wet weight) and common octopus (0.64 μgg−1 wet weight). The majority of the samples analyzed were in compliance with European Union legislation, except for a few cases. The calculated mean weekly intakes of Hg, Cd, and Pb through consumption of the fishery products tested were all below the legislated respective provisional tolerable weekly intakes. In general, the samples analyzed were considered safe to eat with regard to the metal concentrations found and the allowable intakes based on legislation. Nevertheless, the consumption of some species may be of significant importance for consumer health.
Maria Maddalena Storelli; Giovanni Normanno; Grazia Barone; Angela Dambrosio; Luigi Errico; Rita Garofalo; Roberto Giacominelli-Stuffler. Toxic Metals (Hg, Cd, and Pb) in Fishery Products Imported into Italy: Suitability for Human Consumption. Journal of Food Protection 2012, 75, 189 -194.
AMA StyleMaria Maddalena Storelli, Giovanni Normanno, Grazia Barone, Angela Dambrosio, Luigi Errico, Rita Garofalo, Roberto Giacominelli-Stuffler. Toxic Metals (Hg, Cd, and Pb) in Fishery Products Imported into Italy: Suitability for Human Consumption. Journal of Food Protection. 2012; 75 (1):189-194.
Chicago/Turabian StyleMaria Maddalena Storelli; Giovanni Normanno; Grazia Barone; Angela Dambrosio; Luigi Errico; Rita Garofalo; Roberto Giacominelli-Stuffler. 2012. "Toxic Metals (Hg, Cd, and Pb) in Fishery Products Imported into Italy: Suitability for Human Consumption." Journal of Food Protection 75, no. 1: 189-194.
Pit-1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals. In this study the Pit-1/. HinfI and Pit-1/. TaqI loci were investigated using PCR-RFLP approach in a sample of 308 Podolica young bulls. All the possible genotypes for both the SNPs were identified. The allelic frequencies at Pit-1/. TaqI locus were 0.76 (G) and 0.24 (A) while those at Pit-1/. HinfI locus were 0.70 (B) and 0.30 (A). Combined genotypic frequencies and possible haplotypes frequencies were also reported. Moreover the relationships between these SNPs and the growth traits of the Podolica bulls were studied. A positive effect of the Pit-1/. HinfI B allele on growth traits were observed. Furthermore significant statistical results concerning the association between Pit-1/. TaqI and early development (from birth to weaning) were found, with a superiority of the G allele. When in combination, the positive effects of the Pit-1/. HinfI B and the Pit-1/. TaqI G allele were partially confirmed
Maria Selvaggi; Cataldo Dario; Giovanni Normanno; Angela Dambrosio; Marco Dario. Analysis of two pit-1 gene polymorphisms and relationships with growth performance traits in Podolica young bulls. Livestock Science 2011, 138, 308 -312.
AMA StyleMaria Selvaggi, Cataldo Dario, Giovanni Normanno, Angela Dambrosio, Marco Dario. Analysis of two pit-1 gene polymorphisms and relationships with growth performance traits in Podolica young bulls. Livestock Science. 2011; 138 (1-3):308-312.
Chicago/Turabian StyleMaria Selvaggi; Cataldo Dario; Giovanni Normanno; Angela Dambrosio; Marco Dario. 2011. "Analysis of two pit-1 gene polymorphisms and relationships with growth performance traits in Podolica young bulls." Livestock Science 138, no. 1-3: 308-312.
Verocytotoxin-producing Escherichia coli (VTEC) O26 is an emergent pathotype that has caused an increasing number of sporadic cases and outbreaks of gastroenteritis, hemorrhagic colitis, and hemolytic uremic syndrome in the United States and Europe. Many cases are associated with the consumption of milk and undercooked or fermented meats. The stx2 strains of VTEC O26 seem to be more likely to cause human infections than isolates expressing only stx1. The isolation and identification of VTEC O26 from foods is labor intensive and time-consuming. We developed a multiplex PCR (M-PCR) assay for the identification and characterization of E. coli O26 VTEC and its detection in raw milk and ground beef. The method is based on the amplification of the wzx, stx1, and stx2 genes for the simultaneous detection of the O26 antigen and verocytotoxin types 1 and 2. This M-PCR assay had a sensitivity of 108 CFU/ml when applied to a bacterial suspension and of 106 CFU/ml or g when applied to both inoculated milk and minced beef samples. This M-PCR assay also was highly specific, and results were consistently negative for negative controls (nonpathogenic E. coli strains, uninoculated milk and beef samples, and samples inoculated with the nontarget microorganisms). This method could be used for the rapid detection of E. coli O26 VTEC from foods and for the rapid identification and characterization of clinical and environmental isolates.
V. Lorusso; Angela Dambrosio; N. C. Quaglia; Antonio Parisi; G. Lasalandra; G. Mula; S. Virgilio; G. Lucifora; M. Dario; G. Normanno. Development of a Multiplex PCR for Rapid Detection of Verocytotoxin-Producing Escherichia coli O26 in Raw Milk and Ground Beef. Journal of Food Protection 2011, 74, 13 -17.
AMA StyleV. Lorusso, Angela Dambrosio, N. C. Quaglia, Antonio Parisi, G. Lasalandra, G. Mula, S. Virgilio, G. Lucifora, M. Dario, G. Normanno. Development of a Multiplex PCR for Rapid Detection of Verocytotoxin-Producing Escherichia coli O26 in Raw Milk and Ground Beef. Journal of Food Protection. 2011; 74 (1):13-17.
Chicago/Turabian StyleV. Lorusso; Angela Dambrosio; N. C. Quaglia; Antonio Parisi; G. Lasalandra; G. Mula; S. Virgilio; G. Lucifora; M. Dario; G. Normanno. 2011. "Development of a Multiplex PCR for Rapid Detection of Verocytotoxin-Producing Escherichia coli O26 in Raw Milk and Ground Beef." Journal of Food Protection 74, no. 1: 13-17.
Verocytotoxin-producing Escherichia coli (VTEC) are foodborne pathogens that cause outbreaks linked to consumption of meat and raw milk. In this note the authors report results obtained from a survey conducted on minced beef and raw bovine milk samples using a Multiplex PCR (M-PCR) for the detection of eae, stx1, stx2 and hlyA genes as a screening step followed by a colony blot hybridization (CBH) technique for the isolation of the VTEC. Of 100 minced beef and 123 raw milk samples, 13 (13%) and 7 (5.7%) were positive in the M-PCR and among these 9 and 3 strains were isolated using CBH, respectively. All isolates showed the presence of the stx2 gene, single or in association with the other investigated genes. None of the isolates belonged to the O157, O26, O91, O103, O111 and O145 serogroups. The study showed that the use of M-PCR for the screening of samples coupled with a sensitive and specific detection technique, could improve the possibility of detection of VTEC strains in foods. Moreover, the presence of VTEC in minced beef and in raw milk confirms their important role as putative vehicles of infection to humans. Stringent control of these foodstuffs is essential for food safety purposes.
Antonio Parisi; Angela Miccolupo; Gianfranco Santagada; Carmine Pedarra; Angela Dambrosio; Giovanni Normanno. Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization. Food Control 2010, 21, 770 -773.
AMA StyleAntonio Parisi, Angela Miccolupo, Gianfranco Santagada, Carmine Pedarra, Angela Dambrosio, Giovanni Normanno. Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization. Food Control. 2010; 21 (5):770-773.
Chicago/Turabian StyleAntonio Parisi; Angela Miccolupo; Gianfranco Santagada; Carmine Pedarra; Angela Dambrosio; Giovanni Normanno. 2010. "Detection of verocytotoxin-producing Escherichia coli (VTEC) in minced beef and raw milk by colony blot hybridization." Food Control 21, no. 5: 770-773.
Escherichia coli O26 is known as a verocytotoxin-producing E. coli (VTEC) organism that causes severe foodborne diseases such as hemorrhagic colitis and hemolytic uremic syndrome. Although cattle are the most important reservoir of VTEC, only a few reports on the role of water buffalo (Bubalus bubalis) as a reservoir of VTEC and on the presence of these organisms in their milk are available. However, in Southern Italy, where water buffalo are intensively reared, an outbreak of hemolytic uremic syndrome due to E. coli O26 has recently been reported, in which the consumption of typical dairy products was considered to be a common risk factor. The aims of this work were to assess the prevalence of E. coli O26 in raw water buffalo milk, to characterize the virulence gene profiles of the isolates, and to evaluate their phenotypic antimicrobial resistance pattern. Of 160 analyzed samples, 1 (0.6%) tested positive for E. coli O26, and the isolate showed the stx1+/stx2+/eae−/hlyA+ genotypic profile. The strain showed resistance against glycopeptides, macrolides, and penicillins. The presence of VTEC organisms in raw water buffalo milk could be considered to be a potential threat to consumers; however, the strict adherence to the processes used in the preparation of the most common buffalo dairy products could strongly mitigate the foodborne risk. To our knowledge, this article reports the first isolation and characterization of E. coli O26 VTEC in raw water buffalo milk.
Vanessa Lorusso; Angela Dambrosio; Nicoletta Cristiana Quaglia; Antonio Parisi; Giovanna La Salandra; Giuseppe Lucifora; Giuseppina Mula; Sebastiano Virgilio; Leonardo Carosielli; Addolorata Rella; Marco Dario; Giovanni Normanno. Verocytotoxin-Producing Escherichia coli O26 in Raw Water Buffalo (Bubalus bubalis) Milk Products in Italy. Journal of Food Protection 2009, 72, 1705 -1708.
AMA StyleVanessa Lorusso, Angela Dambrosio, Nicoletta Cristiana Quaglia, Antonio Parisi, Giovanna La Salandra, Giuseppe Lucifora, Giuseppina Mula, Sebastiano Virgilio, Leonardo Carosielli, Addolorata Rella, Marco Dario, Giovanni Normanno. Verocytotoxin-Producing Escherichia coli O26 in Raw Water Buffalo (Bubalus bubalis) Milk Products in Italy. Journal of Food Protection. 2009; 72 (8):1705-1708.
Chicago/Turabian StyleVanessa Lorusso; Angela Dambrosio; Nicoletta Cristiana Quaglia; Antonio Parisi; Giovanna La Salandra; Giuseppe Lucifora; Giuseppina Mula; Sebastiano Virgilio; Leonardo Carosielli; Addolorata Rella; Marco Dario; Giovanni Normanno. 2009. "Verocytotoxin-Producing Escherichia coli O26 in Raw Water Buffalo (Bubalus bubalis) Milk Products in Italy." Journal of Food Protection 72, no. 8: 1705-1708.
Helicobacter pylori is an organism widespread in the human population and sometimes responsible for serious illnesses. Since H. pylori has been detected in Italy from an high percentage of sheep milk samples, it has been hypothesized that contaminated milk, may act as a vehicle of transmission of the microorganism to humans. In this work, a Nested Polymerase Chain Reaction approach has been used to detect H. pylori phosphoglucosamine mutase gene (glmM) from sheep, goat and cow milk artificially contaminated with wild H. pylori strains isolated from human gastric biopsies and the reference strain (H. pylori ATCC 43504). The technique showed a high sensitivity of 3 CFU/ml and proved to be both specific and rapid, The authors suggest that it could be used as a sensitive method for a rapid screening of sheep, goat and cow milk samples during the microbiological control of these large consumed foods.
N.C. Quaglia; Angela Dambrosio; G. Normanno; Gaetano Vitale Celano. Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk. Food Control 2009, 20, 119 -123.
AMA StyleN.C. Quaglia, Angela Dambrosio, G. Normanno, Gaetano Vitale Celano. Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk. Food Control. 2009; 20 (2):119-123.
Chicago/Turabian StyleN.C. Quaglia; Angela Dambrosio; G. Normanno; Gaetano Vitale Celano. 2009. "Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk." Food Control 20, no. 2: 119-123.
Helicobacter pylori is an organism widespread in humans and sometimes responsible for serious illnesses, such as gastric and duodenal ulcers, MALToma and even gastric cancer. It has been hypothesized that the infection route by H. pylori involves multiple pathways including food-borne transmission, as the microorganism has been detected from foods such as sheep and cow milk. This work reports the results of a survey conducted in order to investigate the presence of H. pylori in raw goat, sheep and cow milk produced in Southern Italy, employing a Nested Polymerase Chain Reaction (Nested-PCR) assay for the detection of the phosphoglucosamine mutase gene (glmM), as screening method followed by conventional bacteriological isolation. Out of the 400 raw milk samples examined, 139 (34.7%) resulted positive for the presence of glmM gene, but no strains were isolated. In this work H. pylori DNA has been firstly detected from 41 (25.6%) raw goat milk samples. The results deserve further investigations on the contamination source/s of the milk samples and on the major impact that it may have on consumers.
N.C. Quaglia; Angela Dambrosio; G. Normanno; Antonio Parisi; R. Patrono; G. Ranieri; A. Rella; Gaetano Vitale Celano. High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene: A risk of food-borne infection? International Journal of Food Microbiology 2008, 124, 43 -47.
AMA StyleN.C. Quaglia, Angela Dambrosio, G. Normanno, Antonio Parisi, R. Patrono, G. Ranieri, A. Rella, Gaetano Vitale Celano. High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene: A risk of food-borne infection? International Journal of Food Microbiology. 2008; 124 (1):43-47.
Chicago/Turabian StyleN.C. Quaglia; Angela Dambrosio; G. Normanno; Antonio Parisi; R. Patrono; G. Ranieri; A. Rella; Gaetano Vitale Celano. 2008. "High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene: A risk of food-borne infection?" International Journal of Food Microbiology 124, no. 1: 43-47.