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Guzmán Carro-Huerga; Álvaro Rodríguez-González; Sara Mayo-Prieto; Samuel Álvarez-García; Santiago Gutiérrez; Pedro Antonio Casquero Luelmo. USO DE NUTRAGREEN® COMO TRANSPORTADOR COLOIDAL PARA REDUCIR EL USO DE FERTILIZANTES Y PESTICIDAS EN PERAL. Agrárias: Pesquisa e Inovação nas Ciências que Alimentam o Mundo V 2021, 220 -227.
AMA StyleGuzmán Carro-Huerga, Álvaro Rodríguez-González, Sara Mayo-Prieto, Samuel Álvarez-García, Santiago Gutiérrez, Pedro Antonio Casquero Luelmo. USO DE NUTRAGREEN® COMO TRANSPORTADOR COLOIDAL PARA REDUCIR EL USO DE FERTILIZANTES Y PESTICIDAS EN PERAL. Agrárias: Pesquisa e Inovação nas Ciências que Alimentam o Mundo V. 2021; ():220-227.
Chicago/Turabian StyleGuzmán Carro-Huerga; Álvaro Rodríguez-González; Sara Mayo-Prieto; Samuel Álvarez-García; Santiago Gutiérrez; Pedro Antonio Casquero Luelmo. 2021. "USO DE NUTRAGREEN® COMO TRANSPORTADOR COLOIDAL PARA REDUCIR EL USO DE FERTILIZANTES Y PESTICIDAS EN PERAL." Agrárias: Pesquisa e Inovação nas Ciências que Alimentam o Mundo V , no. : 220-227.
The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the volatile organic compound chamber (VOC chamber), specifically designed to perform these experiments. The novel devices were tested using four Trichoderma strains against Fusarium oxysporum and Rhizoctonia solani. We demonstrate that VOC chambers provide higher sensitivity and selectivity between treatments and higher homogeneity of results than the traditional method. VOC chambers are also able to test both vented and non-vented conditions. We prove that ventilation plays a very important role regarding volatile interactions, up to the point that some growth-inhibitory effects observed in closed environments switch to promoting ones when tested in vented conditions. This promoting activity seems to be related to the accumulation of squalene by T. harzianum. The VOC chambers proved to be an easy, homogeneous, flexible, and repeatable method, able to better select microorganisms with high biocontrol activity and to guide the future identification of new bioactive VOCs and their role in microbial interactions.
Samuel Álvarez-García; Sara Mayo-Prieto; Guzmán Carro-Huerga; Álvaro Rodríguez-González; Óscar González-López; Santiago Gutiérrez; Pedro Casquero. Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays. Journal of Fungi 2021, 7, 248 .
AMA StyleSamuel Álvarez-García, Sara Mayo-Prieto, Guzmán Carro-Huerga, Álvaro Rodríguez-González, Óscar González-López, Santiago Gutiérrez, Pedro Casquero. Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays. Journal of Fungi. 2021; 7 (4):248.
Chicago/Turabian StyleSamuel Álvarez-García; Sara Mayo-Prieto; Guzmán Carro-Huerga; Álvaro Rodríguez-González; Óscar González-López; Santiago Gutiérrez; Pedro Casquero. 2021. "Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays." Journal of Fungi 7, no. 4: 248.
Fungal species from the genus Fusarium are important soil-borne pathogens worldwide, causing significant economic losses in diverse crops. The need to find sustainable solutions against this disease has led to the development of new strategies—for instance, the use of biocontrol agents. In this regard, non-pathogenic Fusarium isolates have demonstrated their ability to help other plants withstand subsequent pathogen attacks. In the present work, several Fusarium isolates were evaluated in climatic chambers to identify those presenting low or non-pathogenic behavior. The inoculation with a low-pathogenic isolate of the fungus did not affect the development of the plant, contrary to the results observed in plants inoculated with pathogenic isolates. The expression of defense-related genes was evaluated and compared between plants inoculated with pathogenic and low-pathogenic Fusarium isolates. Low-pathogenic isolates caused a general downregulation of several plant defense-related genes, while pathogenic ones produced an upregulation of these genes. This kind of response to low-pathogenic fungal isolates has been already described for other plant species and fungal pathogens, being related to enhanced tolerance to later pathogen attacks. The results here presented suggest that low-pathogenic F. oxysporum and F. solani isolates may have potential biocontrol activity against bean pathogens via induced and systemic responses in the plant.
Alejandra J. Porteous-Álvarez; Sara Mayo-Prieto; Samuel Álvarez-García; Bonifacio Reinoso; Pedro A. Casquero. Genetic Response of Common Bean to the Inoculation with Indigenous Fusarium Isolates. Journal of Fungi 2020, 6, 228 .
AMA StyleAlejandra J. Porteous-Álvarez, Sara Mayo-Prieto, Samuel Álvarez-García, Bonifacio Reinoso, Pedro A. Casquero. Genetic Response of Common Bean to the Inoculation with Indigenous Fusarium Isolates. Journal of Fungi. 2020; 6 (4):228.
Chicago/Turabian StyleAlejandra J. Porteous-Álvarez; Sara Mayo-Prieto; Samuel Álvarez-García; Bonifacio Reinoso; Pedro A. Casquero. 2020. "Genetic Response of Common Bean to the Inoculation with Indigenous Fusarium Isolates." Journal of Fungi 6, no. 4: 228.
Self-inhibitory processes are a common feature shared by different organisms. One of the main mechanisms involved in these interactions regarding microorganisms is the release of toxic diffusible substances into the environment. These metabolites can exert both antimicrobial effects against other organisms as well as self-inhibitory ones. The in vitro evaluation of these effects against other organisms has been widely used to identify potential biocontrol agents against phytopathogenic microorganisms. In the present study, we performed membrane assays to compare the self-inhibitory effects of soluble metabolites produced by several Trichoderma isolates and their antifungal activity against a phytopathogenic strain of Fusarium oxysporum. The results demonstrated that Trichoderma spp. present a high self-inhibitory activity in vitro, being affected in both their growth rate and the macroscopic structure of their colonies. These effects were highly similar to those exerted against F. oxysporum in the same conditions, showing no significant differences in most cases. Consequently, membrane assays may not be very informative by themselves to assess putative biocontrol capabilities. Therefore, different methods, or a combination of antifungal and self-inhibitory experiments, could be a better approach to evaluate the potential biocontrol activity of microbial strains in order to pre-select them for further in vivo trials.
Samuel Álvarez-García; Sara Mayo-Prieto; Santiago Gutiérrez; Pedro Antonio Casquero. Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum. Journal of Fungi 2020, 6, 176 .
AMA StyleSamuel Álvarez-García, Sara Mayo-Prieto, Santiago Gutiérrez, Pedro Antonio Casquero. Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum. Journal of Fungi. 2020; 6 (3):176.
Chicago/Turabian StyleSamuel Álvarez-García; Sara Mayo-Prieto; Santiago Gutiérrez; Pedro Antonio Casquero. 2020. "Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum." Journal of Fungi 6, no. 3: 176.
The bean weevil, Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), causes severe post-harvest losses in the common bean, Phaseolus vulgaris L. The control of this insect is still poor and involves the use of conventional insecticides. There is an increasing demand in the search for new active substances and products for pest control towards reduction of adverse effects on human health and the environment. The protection of grains with alternative products, such as essential oils, is a possible alternative to meet the needs described above. Therefore, this investigation evaluated the applications of basil, Ocimum basilicum, and citronella, Cymbopogon winterianus, essential oils for A. obtectus control. These essential oils significantly reduced the bean weight losses and the number of beans damaged by A. obtectus at higher doses than 60 or 120 μL/sample. The number of holes per bean did not differ between the doses of basil essential oil, not even at the dose of 60 μL, while it was higher at 120 μL, probably due to a lower capacity of movement of the insects treated with this dose and/or the oil’s direct or indirect effects on the insects. Basil and citronella oils exhibited similar patterns of insecticidal activity over the insect, both directly in adult insects or indirectly over bean seeds. These essential oils affected the development of A. obtectus since the greatest doses applied on beans decreased the emergence of the bean weevil. The results prove the insecticidal capacity of the tested essential oils and hence their potential as active substances against A. obtectus in environmentally low risk pest control strategies. Supplementary trials should be conducted under real storage conditions.
Álvaro Rodríguez-González; Samuel Álvarez-García; Óscar González-López; Franceli Da Silva; Pedro A. Casquero. Insecticidal Properties of Ocimum basilicum and Cymbopogon winterianus against Acanthoscelides obtectus, Insect Pest of the Common Bean (Phaseolus vulgaris, L.). Insects 2019, 10, 151 .
AMA StyleÁlvaro Rodríguez-González, Samuel Álvarez-García, Óscar González-López, Franceli Da Silva, Pedro A. Casquero. Insecticidal Properties of Ocimum basilicum and Cymbopogon winterianus against Acanthoscelides obtectus, Insect Pest of the Common Bean (Phaseolus vulgaris, L.). Insects. 2019; 10 (5):151.
Chicago/Turabian StyleÁlvaro Rodríguez-González; Samuel Álvarez-García; Óscar González-López; Franceli Da Silva; Pedro A. Casquero. 2019. "Insecticidal Properties of Ocimum basilicum and Cymbopogon winterianus against Acanthoscelides obtectus, Insect Pest of the Common Bean (Phaseolus vulgaris, L.)." Insects 10, no. 5: 151.