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Gabriel López-García
Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia, Av, Vicente Andrés Estellés s/n, 46100 Burjassot, Spain

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Paper
Published: 24 November 2020 in Food & Function
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Bovine plasma hydrolysates with a degree of hydrolysis of 19.1% have an iron chelating capacity of 38.5 ± 0.4% and increase the synthesis of ferritin in Caco-2 cells five-fold compared to the control.

ACS Style

Nathalia A. Gómez-Grimaldos; Leidy J. Gómez-Sampedro; José E. Zapata-Montoya; Gabriel López-García; Antonio Cilla; Amparo Alegría-Torán. Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells. Food & Function 2020, 1 .

AMA Style

Nathalia A. Gómez-Grimaldos, Leidy J. Gómez-Sampedro, José E. Zapata-Montoya, Gabriel López-García, Antonio Cilla, Amparo Alegría-Torán. Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells. Food & Function. 2020; ():1.

Chicago/Turabian Style

Nathalia A. Gómez-Grimaldos; Leidy J. Gómez-Sampedro; José E. Zapata-Montoya; Gabriel López-García; Antonio Cilla; Amparo Alegría-Torán. 2020. "Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells." Food & Function , no. : 1.

Proceedings
Published: 09 November 2020 in Proceedings
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Unabsorbed cholesterol, along with that of bile secretions and flaked colon cells, can be metabolized by colonic microbiota. The generated metabolites have been proposed as promoters of colorectal cancer (CRC). In this study, the cytotoxicity (MTT assay) of the main commercially available cholesterol-derived metabolites (coprostanol, cholestanol, coprostanone, and cholestenone) on human colon cancer (Caco-2) and non-tumor (CCD-18Co) cells was evaluated at different physiologically relevant concentrations (9.4–300 µM) and incubation times (24–72 h). In general, the metabolites that most reduced cell viability were coprostanone (54–85% in Caco-2 and 20–81% in CCD- 18Co) and cholestenone (17–91% in Caco-2 and 14–81% in CCD-18Co). These two metabolites are the most hydrophobic, thus reflecting a possible relationship between hydrophobicity and cytotoxicity. Moreover, cholestenone (IC50 at 72 h: 5 ± 1 µg/mL) should be considered cytotoxic on CCD- 18Co cells (non-tumor cells) since it shows an IC50 close to the one considered toxic (<4 µg/mL). Furthermore, CCD-18Co cells are more vulnerable to the cytotoxic effect of cholesterol metabolites. Possible compensatory responses, attenuating the reduction in cell viability caused by cholesterol metabolites, were observed, however these reactions could favor inflammation, resistance to apoptosis, and cellular proliferation, likely contributing to the development of CRC. In conclusion, cholesterol metabolites, mainly the most hydrophobic, could act as promoters of CRC through their cytotoxic activity.

ACS Style

Mussa Makran; Gabriel López-García; Guadalupe Garcia-Llatas; Reyes Barberá; Amparo Alegría; Antonio Cilla. Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis. Proceedings 2020, 70, 56 .

AMA Style

Mussa Makran, Gabriel López-García, Guadalupe Garcia-Llatas, Reyes Barberá, Amparo Alegría, Antonio Cilla. Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis. Proceedings. 2020; 70 (1):56.

Chicago/Turabian Style

Mussa Makran; Gabriel López-García; Guadalupe Garcia-Llatas; Reyes Barberá; Amparo Alegría; Antonio Cilla. 2020. "Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis." Proceedings 70, no. 1: 56.

Journal article
Published: 06 July 2020 in Foods
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Iron deficiencies continue to cause significant health problems in vulnerable populations. A good strategy to combat mineral deficiency includes fortification with iron-binding peptides. This research aims to determine the optimal conditions to hydrolyze red tilapia viscera (RTV) using Alcalase 2.4 L and recovery of iron-binding protein hydrolysate. The result showed that under the optimal hydrolysis condition including pH 10, 60 °C, E/S ratio of 0.306 U/g protein, and substrate concentration of 8 g protein/L, the obtained hydrolysate with 42.5% degree of hydrolysis (RTVH-B), displayed the maximal iron-binding capacity of 67.1 ± 1.9%. Peptide fractionation was performed using ultrafiltration and the p < 0.05) than iron salt in its free form, increased 4.7 times for the Fe2+–RTVH-B complex. This research suggests a potential application of RTVH-B as dietary supplements to improve iron absorption.

ACS Style

Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability. Foods 2020, 9, 883 .

AMA Style

Leidy J. Gómez, Nathalia A. Gómez, José E. Zapata, Gabriel López-García, Antonio Cilla, Amparo Alegría. Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability. Foods. 2020; 9 (7):883.

Chicago/Turabian Style

Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. 2020. "Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability." Foods 9, no. 7: 883.

Journal article
Published: 28 April 2020 in Antioxidants
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The antiproliferative effect of the bioaccessible fractions (BFs) of four hydroponic Brassicaceae microgreens (broccoli, kale, mustard and radish) was evaluated on colon cancer Caco-2 cells vs. normal colon CCD18-Co cells after 24 h treatment with BFs diluted 1:10 v/v in cell culture medium. Their bioactivity was compared with the digestion blank, while the colon cancer chemotherapeutic drug 5-fluorouracil was used as a positive control. Cell viability (mitochondrial enzyme activity assay (MTT test) and Trypan blue test) and mechanisms related to antiproliferative activity (cell cycle, apoptosis/necrosis, mitochondrial membrane potential, reactive oxygen species (ROS) production, Ca2+ and glutathione (GSH) intracellular content) were studied. All microgreen BFs increased ROS and decreased GSH, altering the redox status and causing mitochondrial membrane dissipation followed by a general cell cycle arrest in G2/M and apoptotic cell death via a Ca2+-independent mechanism. As a result, the antioxidant bioactive compounds present in these microgreen species reduced the proliferation of tumoral cells (10 to 12.8% -MTT or 20 to 41.9% -Trypan blue), showing lesser effects with broccoli microgreens, in line with their lower ascorbic acid content and total antioxidant capacity. Therefore, the daily intake of microgreens within a balanced diet could be a preventive nutritional strategy to reduce the burden of chronic degenerative diseases such as colon cancer.

ACS Style

Beatriz De La Fuente; Gabriel López-García; Vicent Máñez; Amparo Alegría; Reyes Barberá; Antonio Cilla. Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition. Antioxidants 2020, 9, 368 .

AMA Style

Beatriz De La Fuente, Gabriel López-García, Vicent Máñez, Amparo Alegría, Reyes Barberá, Antonio Cilla. Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition. Antioxidants. 2020; 9 (5):368.

Chicago/Turabian Style

Beatriz De La Fuente; Gabriel López-García; Vicent Máñez; Amparo Alegría; Reyes Barberá; Antonio Cilla. 2020. "Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition." Antioxidants 9, no. 5: 368.

Journal article
Published: 09 July 2019 in Foods
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Microgreens constitute an emerging class of fresh, healthy foods due to their nutritional composition. In this study the content of minerals and antioxidant bioactive compounds, and for the first time bioaccessibility, were evaluated in broccoli (Brassica oleracea L. var. italica Plenck), green curly kale (Brassica oleracea var. sabellica L.), red mustard (Brassica juncea (L.) Czern.) and radish (Raphanus sativus L.) hydroponic microgreens. Macro- (K, Ca, Mg) and oligo-elements (Fe, Zn), ascorbic acid, total soluble polyphenols, total carotenoids, total anthocyanins, total isothiocyanates and total antioxidant capacity (Trolox Equivalent Antioxidant Capacity and Oxygen Radical Absorbance Capacity) were determined before and after the standardized simulated gastrointestinal digestion process. All microgreens provided relevant amounts of vitamin C (31–56 mg/100 g fresh weight) and total carotenoids (162–224 mg β-carotene/100 g dry weight). Mineral content was comparable to that normally found in hydroponic microgreens and the low potassium levels observed would allow their dietetic recommendation for patients with impaired kidney function. Both total soluble polyphenols and total isothiocyanates were the greatest contributors to the total antioxidant capacity after digestion (43–70% and 31–63% bioaccessibility, respectively) while macroelements showed an important bioaccessibility (34–90%). In general, radish and mustard presented the highest bioaccessibility of bioactive compounds and minerals. Overall, the four hydroponic Brassicaceae microgreens present a wide array of antioxidant bioactive compounds.

ACS Style

Beatriz De La Fuente; Gabriel López-García; Vicent Mañez; Amparo Alegría; Reyes Barberá; Antonio Cilla. Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens. Foods 2019, 8, 250 .

AMA Style

Beatriz De La Fuente, Gabriel López-García, Vicent Mañez, Amparo Alegría, Reyes Barberá, Antonio Cilla. Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens. Foods. 2019; 8 (7):250.

Chicago/Turabian Style

Beatriz De La Fuente; Gabriel López-García; Vicent Mañez; Amparo Alegría; Reyes Barberá; Antonio Cilla. 2019. "Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens." Foods 8, no. 7: 250.

Journal article
Published: 01 June 2019 in Food Research International
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The antioxidant capacity of red tilapia viscera hydrolysates (RTVH) with different degrees of hydrolysis (DH) as well as their ultrafiltration membrane fractions, were analyzed using different chemical assays. Their protective effects against oxidative stress were evaluated using H2O2-stressed human intestinal differentiated Caco-2. The highest antioxidant capacity was obtained with a DH of 42.5% (RTVH-A) and its <1 kDa fraction (FRTVH-V). RTVH-A and FRTVH-V did not show cytotoxic effects at a concentration of ≤0.5 mg/mL,prevented the decrease in cell viability, and suppressed intracellular reactive oxygen species (ROS) accumulation induced by H2O2. However, pretreatment with RTVH-A after adding H2O2, showed a greater decrease in glutathione levels. Moreover, FRTVH-V allowed for a recovery close to that of control levels of cell proportions in the G1 and G2/M cell cycle phases; and a decrease in the cell proportion in late apoptosis. These results suggest that RTVH-A and FRTVH-V can be beneficial ingredients with antioxidant properties and can have protective effects against ROS-mediated intestinal injuries.

ACS Style

Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates. Food Research International 2019, 120, 52 -61.

AMA Style

Leidy J. Gómez, Nathalia A. Gómez, José E. Zapata, Gabriel López-García, Antonio Cilla, Amparo Alegría. In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates. Food Research International. 2019; 120 ():52-61.

Chicago/Turabian Style

Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. 2019. "In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates." Food Research International 120, no. : 52-61.

Journal article
Published: 04 April 2019 in Foods
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The potential anti-inflammatory effect of plant sterols (PS) enriched milk-based fruit beverages (PS, 1 g/100 mL) (MfB) with/without galactooligosaccharides (GOS, 2 g/100 mL) (MfB-G) in an experimental mice model of chronic ulcerative colitis was evaluated. Beverages were orally administered to mice every day by gavage to achieve PS and GOS doses of 35 and 90 mg/kg, respectively, and experimental colitis was induced by giving mice drinking water ad libitum containing 2% (w/v) dextran sulphate sodium (DSS) for 7 days, alternating with periods without DSS up to the end of the study (56 days). MfB beverage showed significant reduction of symptoms associated to ulcerative colitis and improved the colon shortening and mucosal colonic damage, but it was not able to reduce the increase of myeloperoxidase levels produced by DSS. MfB-G showed higher incidence of bloody feces and loss of stool consistency than MfB, as well as high levels of immune cells infiltration in colon tissue and myeloperoxidase. Therefore, PS-enriched milk-based fruit beverage could be an interesting healthy food to extend the remission periods of the diseases and the need to evaluate, in a pre-clinical model, the anti-inflammatory effect of the combination of bioactive compounds in the context of a whole food matrix.

ACS Style

Gabriel López-García; Antonio Cilla; Reyes Barberá; Amparo Alegría; María C. Recio. Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model. Foods 2019, 8, 114 .

AMA Style

Gabriel López-García, Antonio Cilla, Reyes Barberá, Amparo Alegría, María C. Recio. Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model. Foods. 2019; 8 (4):114.

Chicago/Turabian Style

Gabriel López-García; Antonio Cilla; Reyes Barberá; Amparo Alegría; María C. Recio. 2019. "Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model." Foods 8, no. 4: 114.