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The aim was to evaluate the antiproliferative activity of bioaccessible fractions (BFs) obtained by the internationally standardized INFOGEST static digestion method to Sicilian honeys of three distinct floral origins (Sulla, Thistle, Dill) and the Manuka honey (gold standard), comparing their effect based on total polyphenols content (TPC). Differentiated CaCo-2 cells (intestinal-like) and non-differentiated CaCo-2 and HCT-116 colonic tumor-like cells were incubated for 24h with BFs of honeys to test viability, apoptosis, mitochondrial membrane potential-MMP, ROS and cell cycle. TPC after digestion ranked: Dill >Thistle >Sulla>Manuka. No decrease in cell viability in differentiated CaCo-2 cells was observed, while a reduction to 25-85% (CaCo-2) and 20-80% (HCT-116) viability was obtained. This descent in viability was caused by a cell cycle block with an increase in apoptosis through dissipation of MMP and raise in ROS levels, being Thistle and Dill, the most effective honeys followed by Sulla and finally Manuka, in agreement with TPC after digestion.
Antonio Cilla; Gabriel López‐García; Reyes Barberá; Anna Frazzitta; Ignazio Restivo; Luisa Tesoriere; Alessandro Attanzio. Antiproliferative effects of bioaccessible fractions of honeys from Sicilian black honeybee ( Apis mellifera ssp. sicula ) on human colorectal carcinoma cells. International Journal of Food Science & Technology 2021, 1 .
AMA StyleAntonio Cilla, Gabriel López‐García, Reyes Barberá, Anna Frazzitta, Ignazio Restivo, Luisa Tesoriere, Alessandro Attanzio. Antiproliferative effects of bioaccessible fractions of honeys from Sicilian black honeybee ( Apis mellifera ssp. sicula ) on human colorectal carcinoma cells. International Journal of Food Science & Technology. 2021; ():1.
Chicago/Turabian StyleAntonio Cilla; Gabriel López‐García; Reyes Barberá; Anna Frazzitta; Ignazio Restivo; Luisa Tesoriere; Alessandro Attanzio. 2021. "Antiproliferative effects of bioaccessible fractions of honeys from Sicilian black honeybee ( Apis mellifera ssp. sicula ) on human colorectal carcinoma cells." International Journal of Food Science & Technology , no. : 1.
Bovine plasma hydrolysates with a degree of hydrolysis of 19.1% have an iron chelating capacity of 38.5 ± 0.4% and increase the synthesis of ferritin in Caco-2 cells five-fold compared to the control.
Nathalia A. Gómez-Grimaldos; Leidy J. Gómez-Sampedro; José E. Zapata-Montoya; Gabriel López-García; Antonio Cilla; Amparo Alegría-Torán. Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells. Food & Function 2020, 1 .
AMA StyleNathalia A. Gómez-Grimaldos, Leidy J. Gómez-Sampedro, José E. Zapata-Montoya, Gabriel López-García, Antonio Cilla, Amparo Alegría-Torán. Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells. Food & Function. 2020; ():1.
Chicago/Turabian StyleNathalia A. Gómez-Grimaldos; Leidy J. Gómez-Sampedro; José E. Zapata-Montoya; Gabriel López-García; Antonio Cilla; Amparo Alegría-Torán. 2020. "Bovine plasma hydrolysates’ iron chelating capacity and its potentiating effect on ferritin synthesis in Caco-2 cells." Food & Function , no. : 1.
Unabsorbed cholesterol, along with that of bile secretions and flaked colon cells, can be metabolized by colonic microbiota. The generated metabolites have been proposed as promoters of colorectal cancer (CRC). In this study, the cytotoxicity (MTT assay) of the main commercially available cholesterol-derived metabolites (coprostanol, cholestanol, coprostanone, and cholestenone) on human colon cancer (Caco-2) and non-tumor (CCD-18Co) cells was evaluated at different physiologically relevant concentrations (9.4–300 µM) and incubation times (24–72 h). In general, the metabolites that most reduced cell viability were coprostanone (54–85% in Caco-2 and 20–81% in CCD- 18Co) and cholestenone (17–91% in Caco-2 and 14–81% in CCD-18Co). These two metabolites are the most hydrophobic, thus reflecting a possible relationship between hydrophobicity and cytotoxicity. Moreover, cholestenone (IC50 at 72 h: 5 ± 1 µg/mL) should be considered cytotoxic on CCD- 18Co cells (non-tumor cells) since it shows an IC50 close to the one considered toxic (<4 µg/mL). Furthermore, CCD-18Co cells are more vulnerable to the cytotoxic effect of cholesterol metabolites. Possible compensatory responses, attenuating the reduction in cell viability caused by cholesterol metabolites, were observed, however these reactions could favor inflammation, resistance to apoptosis, and cellular proliferation, likely contributing to the development of CRC. In conclusion, cholesterol metabolites, mainly the most hydrophobic, could act as promoters of CRC through their cytotoxic activity.
Mussa Makran; Gabriel López-García; Guadalupe Garcia-Llatas; Reyes Barberá; Amparo Alegría; Antonio Cilla. Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis. Proceedings 2020, 70, 56 .
AMA StyleMussa Makran, Gabriel López-García, Guadalupe Garcia-Llatas, Reyes Barberá, Amparo Alegría, Antonio Cilla. Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis. Proceedings. 2020; 70 (1):56.
Chicago/Turabian StyleMussa Makran; Gabriel López-García; Guadalupe Garcia-Llatas; Reyes Barberá; Amparo Alegría; Antonio Cilla. 2020. "Cytotoxic Effect of Cholesterol Metabolites on Human Colonic Tumor (Caco-2) and Non-Tumor (CCD-18Co) Cells and Their Potential Implication in Colorectal Carcinogenesis." Proceedings 70, no. 1: 56.
HPP can be recommended as a suitable technology to retain or indeed increase antioxidant bioactives and their cytoprotective activity in Clementine mandarin juices after gastrointestinal digestion.
Antonio Cilla; María J. Rodrigo; Begoña De Ancos; Concepción Sánchez-Moreno; M. Pilar Cano; Lorenzo Zacarías; Reyes Barberá; Amparo Alegría. Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells. Food & Function 2020, 11, 8951 -8962.
AMA StyleAntonio Cilla, María J. Rodrigo, Begoña De Ancos, Concepción Sánchez-Moreno, M. Pilar Cano, Lorenzo Zacarías, Reyes Barberá, Amparo Alegría. Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells. Food & Function. 2020; 11 (10):8951-8962.
Chicago/Turabian StyleAntonio Cilla; María J. Rodrigo; Begoña De Ancos; Concepción Sánchez-Moreno; M. Pilar Cano; Lorenzo Zacarías; Reyes Barberá; Amparo Alegría. 2020. "Impact of high-pressure processing on the stability and bioaccessibility of bioactive compounds in Clementine mandarin juice and its cytoprotective effect on Caco-2 cells." Food & Function 11, no. 10: 8951-8962.
Iron deficiencies continue to cause significant health problems in vulnerable populations. A good strategy to combat mineral deficiency includes fortification with iron-binding peptides. This research aims to determine the optimal conditions to hydrolyze red tilapia viscera (RTV) using Alcalase 2.4 L and recovery of iron-binding protein hydrolysate. The result showed that under the optimal hydrolysis condition including pH 10, 60 °C, E/S ratio of 0.306 U/g protein, and substrate concentration of 8 g protein/L, the obtained hydrolysate with 42.5% degree of hydrolysis (RTVH-B), displayed the maximal iron-binding capacity of 67.1 ± 1.9%. Peptide fractionation was performed using ultrafiltration and the p < 0.05) than iron salt in its free form, increased 4.7 times for the Fe2+–RTVH-B complex. This research suggests a potential application of RTVH-B as dietary supplements to improve iron absorption.
Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability. Foods 2020, 9, 883 .
AMA StyleLeidy J. Gómez, Nathalia A. Gómez, José E. Zapata, Gabriel López-García, Antonio Cilla, Amparo Alegría. Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability. Foods. 2020; 9 (7):883.
Chicago/Turabian StyleLeidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. 2020. "Optimization of the Red Tilapia (Oreochromis spp.) Viscera Hydrolysis for Obtaining Iron-Binding Peptides and Evaluation of In Vitro Iron Bioavailability." Foods 9, no. 7: 883.
Olive (Olea europaea L.) leaves and tea (Camellia sinensis) are rich sources of bioactive compounds, especially polyphenols. Our previous studies have evidenced the potential use of Saccharomyces cerevisiae as a natural delivery system for these antioxidants and a means to improve their bioaccessibility in the human gut. In the present work, the antiproliferative effect of green tea (GT), black tea (BT) and olive leaves (OL) infusions and suspensions of S. cerevisiae were evaluated, for the first time, in human colon cancer cells (Caco-2) after biosorption and in vitro gastrointestinal digestion. The bioaccessible fractions (BF) were not overtly cytotoxic, not affecting cell viability. ROS and mitochondrial membrane potential changes (Δψm) values were reduced compared with control cells. Moreover, all the BF after biosorption induced a significant (p < 0.05) increase in cell proportions in S-phase. The arrest of the cell cycle was reversible without induction of apoptosis, suggesting that the biosorbed phenolics in both infusions and suspensions act as cytostatic agents.
Hanène Jilani; Antonio Cilla; Reyes Barberá; Moktar Hamdi. Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-2 cells. Food Research International 2020, 136, 109317 .
AMA StyleHanène Jilani, Antonio Cilla, Reyes Barberá, Moktar Hamdi. Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-2 cells. Food Research International. 2020; 136 ():109317.
Chicago/Turabian StyleHanène Jilani; Antonio Cilla; Reyes Barberá; Moktar Hamdi. 2020. "Antiproliferative activity of green, black tea and olive leaves polyphenols subjected to biosorption and in vitro gastrointestinal digestion in Caco-2 cells." Food Research International 136, no. : 109317.
Bioactive peptides derived from food protein sources have been widely studied in the last years, and scientific researchers have been proving their role in human health, beyond their nutritional value. Several bioactivities have been attributed to these peptides, such as immunomodulatory, antimicrobial, antioxidant, antihypertensive, and opioid. Among them, metal-binding capacity has gained prominence. Mineral chelating peptides have shown potential to be applied in food products so as to decrease mineral deficiencies since peptide-metal complexes could enhance their bioavailability. Furthermore, many studies have been investigating their potential to decrease the Fe pro-oxidant effect by forming a stable structure with the metal and avoiding its interaction with other food constituents. These complexes can be formed during gastrointestinal digestion or can be synthesized prior to intake, with the aim to protect the mineral through the gastrointestinal tract. This review addresses: (i) the amino acid residues for metal-binding peptides and their main protein sources, (ii) peptide-metal complexation prior to or during gastrointestinal digestion, (iii) the function of metal (especially Fe, Ca, and Zn)-binding peptides on the metal bioavailability and (iv) their reactivity and possible pro-oxidant and side effects.
Maria Elisa Caetano-Silva; Flavia Maria Netto; Maria Teresa Bertoldo-Pacheco; Amparo Alegría; Antonio Cilla. Peptide-metal complexes: obtention and role in increasing bioavailability and decreasing the pro-oxidant effect of minerals. Critical Reviews in Food Science and Nutrition 2020, 61, 1470 -1489.
AMA StyleMaria Elisa Caetano-Silva, Flavia Maria Netto, Maria Teresa Bertoldo-Pacheco, Amparo Alegría, Antonio Cilla. Peptide-metal complexes: obtention and role in increasing bioavailability and decreasing the pro-oxidant effect of minerals. Critical Reviews in Food Science and Nutrition. 2020; 61 (9):1470-1489.
Chicago/Turabian StyleMaria Elisa Caetano-Silva; Flavia Maria Netto; Maria Teresa Bertoldo-Pacheco; Amparo Alegría; Antonio Cilla. 2020. "Peptide-metal complexes: obtention and role in increasing bioavailability and decreasing the pro-oxidant effect of minerals." Critical Reviews in Food Science and Nutrition 61, no. 9: 1470-1489.
The antiproliferative effect of the bioaccessible fractions (BFs) of four hydroponic Brassicaceae microgreens (broccoli, kale, mustard and radish) was evaluated on colon cancer Caco-2 cells vs. normal colon CCD18-Co cells after 24 h treatment with BFs diluted 1:10 v/v in cell culture medium. Their bioactivity was compared with the digestion blank, while the colon cancer chemotherapeutic drug 5-fluorouracil was used as a positive control. Cell viability (mitochondrial enzyme activity assay (MTT test) and Trypan blue test) and mechanisms related to antiproliferative activity (cell cycle, apoptosis/necrosis, mitochondrial membrane potential, reactive oxygen species (ROS) production, Ca2+ and glutathione (GSH) intracellular content) were studied. All microgreen BFs increased ROS and decreased GSH, altering the redox status and causing mitochondrial membrane dissipation followed by a general cell cycle arrest in G2/M and apoptotic cell death via a Ca2+-independent mechanism. As a result, the antioxidant bioactive compounds present in these microgreen species reduced the proliferation of tumoral cells (10 to 12.8% -MTT or 20 to 41.9% -Trypan blue), showing lesser effects with broccoli microgreens, in line with their lower ascorbic acid content and total antioxidant capacity. Therefore, the daily intake of microgreens within a balanced diet could be a preventive nutritional strategy to reduce the burden of chronic degenerative diseases such as colon cancer.
Beatriz De La Fuente; Gabriel López-García; Vicent Máñez; Amparo Alegría; Reyes Barberá; Antonio Cilla. Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition. Antioxidants 2020, 9, 368 .
AMA StyleBeatriz De La Fuente, Gabriel López-García, Vicent Máñez, Amparo Alegría, Reyes Barberá, Antonio Cilla. Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition. Antioxidants. 2020; 9 (5):368.
Chicago/Turabian StyleBeatriz De La Fuente; Gabriel López-García; Vicent Máñez; Amparo Alegría; Reyes Barberá; Antonio Cilla. 2020. "Antiproliferative Effect of Bioaccessible Fractions of Four Brassicaceae Microgreens on Human Colon Cancer Cells Linked to Their Phytochemical Composition." Antioxidants 9, no. 5: 368.
This study evaluates the impact of two plant sterol (PS) enriched fruit beverages (0.6 g /100 ml), without (MfB) or with GOS (MfB-G) (1.2 g/100 ml) on the resistance against oxidative stress induced by non-conventional (cholesterol oxidation products (COPs)) and conventional (H2O2) oxidant compounds, as well as their impact on C. elegans longevity. Nematodes fed with both beverages (0.005–0.25%, v/v) showed similar improved oxidative stress resistance against COPs and H2O2. This effect was dependent on daf-16 transcription factor, although GOS showed an additional beneficial effect independent to daf-16. In addition, both beverages extended nematode lifespan, independently of the presence of GOS. Longevity assays using daf-16 and daf-2 mutant strains revealed that the observed effect was potentially linked to the insulin-IGF-1 pathway. These results provide new in vivo evidence for the potential use of PS enriched fruit beverages in the prevention of oxidative stress underlying many diseases.
G. López-García; A. Cilla; R. Barberá; S. Genovés; P. Martorell; A. Alegría. Effect of plant sterol and galactooligosaccharides enriched beverages on oxidative stress and longevity in Caenorhabditis elegans. Journal of Functional Foods 2019, 65, 103747 .
AMA StyleG. López-García, A. Cilla, R. Barberá, S. Genovés, P. Martorell, A. Alegría. Effect of plant sterol and galactooligosaccharides enriched beverages on oxidative stress and longevity in Caenorhabditis elegans. Journal of Functional Foods. 2019; 65 ():103747.
Chicago/Turabian StyleG. López-García; A. Cilla; R. Barberá; S. Genovés; P. Martorell; A. Alegría. 2019. "Effect of plant sterol and galactooligosaccharides enriched beverages on oxidative stress and longevity in Caenorhabditis elegans." Journal of Functional Foods 65, no. : 103747.
Plant sterol (PS) (1 g/100 mL) enriched milk-based fruit beverages with or without galactooligosaccharides (GOS) (1.8 g/100 mL) were used in differentiated Caco-2 cells. Their potential cytopreventive effect against oxidative stress induced by cholesterol oxidation products (COPs) and their anti-inflammatory properties were evaluated. Preincubation (24 h) with bioaccessible fractions from beverages without and with GOS (MfB and MfB-G) completely prevented the COPs (60 μM/4 h) induced oxidative stress independent to GOS presence with exception to calcium influx and GSH content, where a partial protective effect was observed. Besides, MfB produced a significant (p < 0.05) reduction of IL-8 (40%) and IL-6 (50%) after IL-1β-induction (1 ng/mL/24 h) through the inhibition of NF-κB p65 translocation into the nucleus (16%) compared to control cells, while GOS presence compromised their anti-inflammatory effect. Therefore, PS-enriched milk-based fruit beverage could be an interesting strategy to prevent intestinal injury produced by COPs and to attenuate the pro-inflammatory process in intestinal human diseases. GOS addition had no extra beneficial antioxidant effect and even reduced their anti-inflammatory properties.
Gabriel López-García; Antonio Cilla; Reyes Barbera; Amparo Alegria. Anti-Inflammatory and Cytoprotective Effect of Plant Sterol and Galactooligosaccharides-Enriched Beverages in Caco-2 Cells. Journal of Agricultural and Food Chemistry 2019, 68, 1862 -1870.
AMA StyleGabriel López-García, Antonio Cilla, Reyes Barbera, Amparo Alegria. Anti-Inflammatory and Cytoprotective Effect of Plant Sterol and Galactooligosaccharides-Enriched Beverages in Caco-2 Cells. Journal of Agricultural and Food Chemistry. 2019; 68 (7):1862-1870.
Chicago/Turabian StyleGabriel López-García; Antonio Cilla; Reyes Barbera; Amparo Alegria. 2019. "Anti-Inflammatory and Cytoprotective Effect of Plant Sterol and Galactooligosaccharides-Enriched Beverages in Caco-2 Cells." Journal of Agricultural and Food Chemistry 68, no. 7: 1862-1870.
Microgreens constitute an emerging class of fresh, healthy foods due to their nutritional composition. In this study the content of minerals and antioxidant bioactive compounds, and for the first time bioaccessibility, were evaluated in broccoli (Brassica oleracea L. var. italica Plenck), green curly kale (Brassica oleracea var. sabellica L.), red mustard (Brassica juncea (L.) Czern.) and radish (Raphanus sativus L.) hydroponic microgreens. Macro- (K, Ca, Mg) and oligo-elements (Fe, Zn), ascorbic acid, total soluble polyphenols, total carotenoids, total anthocyanins, total isothiocyanates and total antioxidant capacity (Trolox Equivalent Antioxidant Capacity and Oxygen Radical Absorbance Capacity) were determined before and after the standardized simulated gastrointestinal digestion process. All microgreens provided relevant amounts of vitamin C (31–56 mg/100 g fresh weight) and total carotenoids (162–224 mg β-carotene/100 g dry weight). Mineral content was comparable to that normally found in hydroponic microgreens and the low potassium levels observed would allow their dietetic recommendation for patients with impaired kidney function. Both total soluble polyphenols and total isothiocyanates were the greatest contributors to the total antioxidant capacity after digestion (43–70% and 31–63% bioaccessibility, respectively) while macroelements showed an important bioaccessibility (34–90%). In general, radish and mustard presented the highest bioaccessibility of bioactive compounds and minerals. Overall, the four hydroponic Brassicaceae microgreens present a wide array of antioxidant bioactive compounds.
Beatriz De La Fuente; Gabriel López-García; Vicent Mañez; Amparo Alegría; Reyes Barberá; Antonio Cilla. Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens. Foods 2019, 8, 250 .
AMA StyleBeatriz De La Fuente, Gabriel López-García, Vicent Mañez, Amparo Alegría, Reyes Barberá, Antonio Cilla. Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens. Foods. 2019; 8 (7):250.
Chicago/Turabian StyleBeatriz De La Fuente; Gabriel López-García; Vicent Mañez; Amparo Alegría; Reyes Barberá; Antonio Cilla. 2019. "Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens." Foods 8, no. 7: 250.
The antioxidant capacity of red tilapia viscera hydrolysates (RTVH) with different degrees of hydrolysis (DH) as well as their ultrafiltration membrane fractions, were analyzed using different chemical assays. Their protective effects against oxidative stress were evaluated using H2O2-stressed human intestinal differentiated Caco-2. The highest antioxidant capacity was obtained with a DH of 42.5% (RTVH-A) and its <1 kDa fraction (FRTVH-V). RTVH-A and FRTVH-V did not show cytotoxic effects at a concentration of ≤0.5 mg/mL,prevented the decrease in cell viability, and suppressed intracellular reactive oxygen species (ROS) accumulation induced by H2O2. However, pretreatment with RTVH-A after adding H2O2, showed a greater decrease in glutathione levels. Moreover, FRTVH-V allowed for a recovery close to that of control levels of cell proportions in the G1 and G2/M cell cycle phases; and a decrease in the cell proportion in late apoptosis. These results suggest that RTVH-A and FRTVH-V can be beneficial ingredients with antioxidant properties and can have protective effects against ROS-mediated intestinal injuries.
Leidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates. Food Research International 2019, 120, 52 -61.
AMA StyleLeidy J. Gómez, Nathalia A. Gómez, José E. Zapata, Gabriel López-García, Antonio Cilla, Amparo Alegría. In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates. Food Research International. 2019; 120 ():52-61.
Chicago/Turabian StyleLeidy J. Gómez; Nathalia A. Gómez; José E. Zapata; Gabriel López-García; Antonio Cilla; Amparo Alegría. 2019. "In-vitro antioxidant capacity and cytoprotective/cytotoxic effects upon Caco-2 cells of red tilapia (Oreochromis spp.) viscera hydrolysates." Food Research International 120, no. : 52-61.
The potential anti-inflammatory effect of plant sterols (PS) enriched milk-based fruit beverages (PS, 1 g/100 mL) (MfB) with/without galactooligosaccharides (GOS, 2 g/100 mL) (MfB-G) in an experimental mice model of chronic ulcerative colitis was evaluated. Beverages were orally administered to mice every day by gavage to achieve PS and GOS doses of 35 and 90 mg/kg, respectively, and experimental colitis was induced by giving mice drinking water ad libitum containing 2% (w/v) dextran sulphate sodium (DSS) for 7 days, alternating with periods without DSS up to the end of the study (56 days). MfB beverage showed significant reduction of symptoms associated to ulcerative colitis and improved the colon shortening and mucosal colonic damage, but it was not able to reduce the increase of myeloperoxidase levels produced by DSS. MfB-G showed higher incidence of bloody feces and loss of stool consistency than MfB, as well as high levels of immune cells infiltration in colon tissue and myeloperoxidase. Therefore, PS-enriched milk-based fruit beverage could be an interesting healthy food to extend the remission periods of the diseases and the need to evaluate, in a pre-clinical model, the anti-inflammatory effect of the combination of bioactive compounds in the context of a whole food matrix.
Gabriel López-García; Antonio Cilla; Reyes Barberá; Amparo Alegría; María C. Recio. Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model. Foods 2019, 8, 114 .
AMA StyleGabriel López-García, Antonio Cilla, Reyes Barberá, Amparo Alegría, María C. Recio. Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model. Foods. 2019; 8 (4):114.
Chicago/Turabian StyleGabriel López-García; Antonio Cilla; Reyes Barberá; Amparo Alegría; María C. Recio. 2019. "Effect of a Milk-Based Fruit Beverage Enriched with Plant Sterols and/or Galactooligosaccharides in a Murine Chronic Colitis Model." Foods 8, no. 4: 114.
The aim of this study was to assess the effect of temperature, solvent (hydroethanolic mixtures) and pH on the recovery of individual phenolic compounds from “horchata” by-products. These parameters were optimized by response surface methodology and triple-TOF-LC-MS-MS was selected as the analytical tool to identify and quantify the individual compounds. The optimum extraction conditions were 50% ethanol, 35 °C and pH 2.5, which resulted in values of 222.6 mg gallic acid equivalents (GAE)/100 g dry matter and 1948.1 µM trolox equivalent (TE)/g of dry matter for total phenolic content (TPC) and trolox equivalent antioxidant capacity (TEAC), respectively. The extraction of phenolic compounds by the conventional solvent method with agitation was influenced by temperature (p = 0.0073), and more strongly, by the content of ethanol in the extraction solution (p = 0.0007) while the pH did not show a great impact (p = 0.7961). On the other hand, the extraction of phenolic acids was affected by temperature (p = 0.0003) and by ethanol amount (p < 0.0001) but not by the pH values (p = 0.53). In addition, the percentage of ethanol influenced notably the extraction of both 4-vinylphenol (p = 0.0002) and the hydroxycinnamic acids (p = 0.0039). Finally, the main individual phenolic extracted with hydroethanolic mixtures was 4-vinylphenol (303.3 μg/kg DW) followed by spinacetin3-O-glucosyl-(1→6)-glucoside (86.2 μg/kg DW) and sinensetin (77.8 μg/kg DW).
Elena Roselló-Soto; Francisco J. Martí-Quijal; Antonio Cilla; Paulo Eduardo Sichetti Munekata; Jose M. Lorenzo; Fabienne Remize; Francisco J. Barba. Influence of Temperature, Solvent and pH on the Selective Extraction of Phenolic Compounds from Tiger Nuts by-Products: Triple-TOF-LC-MS-MS Characterization. Molecules 2019, 24, 797 .
AMA StyleElena Roselló-Soto, Francisco J. Martí-Quijal, Antonio Cilla, Paulo Eduardo Sichetti Munekata, Jose M. Lorenzo, Fabienne Remize, Francisco J. Barba. Influence of Temperature, Solvent and pH on the Selective Extraction of Phenolic Compounds from Tiger Nuts by-Products: Triple-TOF-LC-MS-MS Characterization. Molecules. 2019; 24 (4):797.
Chicago/Turabian StyleElena Roselló-Soto; Francisco J. Martí-Quijal; Antonio Cilla; Paulo Eduardo Sichetti Munekata; Jose M. Lorenzo; Fabienne Remize; Francisco J. Barba. 2019. "Influence of Temperature, Solvent and pH on the Selective Extraction of Phenolic Compounds from Tiger Nuts by-Products: Triple-TOF-LC-MS-MS Characterization." Molecules 24, no. 4: 797.
Breast, prostate, and colon cancer account for the highest incidents of all cancers in the human population. Plant extracts, used as food or in traditional medicine, contain different phytochemicals (including phytosterols) that could exert anticarcinogenic effects. Plant extracts comprise a complex series of bioactive compounds, with a low percentage of phytosterols (up to 6%). Antiproliferative activity is mainly evaluated on the basis of IC50, with mechanistic action studies being scarce. However, it is difficult to attribute anticarcinogenic effects only to the phytosterols present in plant extracts – although their contribution cannot be neglected. Regarding phytosterols isolated from plant extracts, stigmasterol has been shown to display the greatest antiproliferative action, followed by β‐sitosterol. Morphological changes with chromatin condensation and nuclear fragmentation, deregulation of the cell cycle, and apoptosis are the main mechanisms involved. The few published studies involving induced rat mammary tumor models have found phytosterols to reduce tumor volume and mass, with an increase in survival. This chapter reviews the activity of plant extracts containing phytosterols or of isolated phytosterols obtained from plant extracts upon breast, prostate, and colon cancer.
Gabriel López-García; Amparo Alegría; Reyes Barberá; Antonio Cilla. Antiproliferative Effects and Mechanism of Action of Phytosterols Derived from Bioactive Plant Extracts. Nutraceuticals and Natural Product Derivatives 2018, 145 -165.
AMA StyleGabriel López-García, Amparo Alegría, Reyes Barberá, Antonio Cilla. Antiproliferative Effects and Mechanism of Action of Phytosterols Derived from Bioactive Plant Extracts. Nutraceuticals and Natural Product Derivatives. 2018; ():145-165.
Chicago/Turabian StyleGabriel López-García; Amparo Alegría; Reyes Barberá; Antonio Cilla. 2018. "Antiproliferative Effects and Mechanism of Action of Phytosterols Derived from Bioactive Plant Extracts." Nutraceuticals and Natural Product Derivatives , no. : 145-165.
Cigarette smoking has been linked with oxidative stress and inflammation. In turn, eryptosis, the suicidal erythrocyte death similar to apoptosis that can be triggered by oxidative stress, has been associated with chronic inflammatory diseases including atherosclerosis. However, the link between smoking and eryptosis has not been explored so far. The aim of the present study was to determine the level of eryptotic erythrocytes in healthy male smokers (n = 21) compared to non-smokers (n = 21) and assess its relationship with systemic inflammation (CRP) as well as with antioxidant defense (GSH) and their resistance to ex-vivo induced hemolysis. Smoking caused an increase in phosphatidylserine translocation outside the erythrocyte membrane (hallmark of eryptosis), significantly correlated to the plasma level of CRP (r = 0.546) and GSH concentration in erythrocytes (r=-0.475). With respect to non-smokers, smokers show a marginal increase of total leucocytes and erythrocyte volume, no modifications of the RBC resistance to oxidative stress-induced hemolysis and hematological and lipid parameters unvaried. We conclude that the inflammatory status (high CRP levels) and RBC oxidative stress (low GSH levels) caused by cigarette smoking are associated with an increase of eryptotic erythrocytes, a yet unknown relationship potentially involved with atherosclerosis and cardiovascular disease in smokers.
Alessandro Attanzio; Anna Frazzitta; Sonya Vasto; Luisa Tesoriere; Anna Maria Pintaudi; Maria Antonia Livrea; Antonio Cilla; Mario Allegra. Increased eryptosis in smokers is associated with the antioxidant status and C-reactive protein levels. Toxicology 2018, 411, 43 -48.
AMA StyleAlessandro Attanzio, Anna Frazzitta, Sonya Vasto, Luisa Tesoriere, Anna Maria Pintaudi, Maria Antonia Livrea, Antonio Cilla, Mario Allegra. Increased eryptosis in smokers is associated with the antioxidant status and C-reactive protein levels. Toxicology. 2018; 411 ():43-48.
Chicago/Turabian StyleAlessandro Attanzio; Anna Frazzitta; Sonya Vasto; Luisa Tesoriere; Anna Maria Pintaudi; Maria Antonia Livrea; Antonio Cilla; Mario Allegra. 2018. "Increased eryptosis in smokers is associated with the antioxidant status and C-reactive protein levels." Toxicology 411, no. : 43-48.
Dietary interventions may effectively control cancer development, with phytosterols (PS) being a class of cancer chemopreventive dietary phytochemicals. The present study, for the first time, evaluates the antiproliferative effects of a PS-ingredient used for the enrichment of several foods and its main PS, β-sitosterol, at physiological serum levels, in the most prevalent cancer cells in women (breast (MCF-7), colon (HCT116) and cervical (HeLa)). In all three cell lines, these compounds induced significant cell viability reduction without a clear time- and dose-dependent response. Moreover, all treatments produced apoptotic cell death with the induction of DNA fragmentation through the appearance of a sub-G1 cell population. Thus, the use of PS as functional ingredients in the development of PS-enriched foods could exert a potential preventive effect against human breast, colon and cervical cancer, although further in vivo studies are required to confirm our preclinical findings.
Andrea Alvarez-Sala; Alessandro Attanzio; Luisa Tesoriere; Guadalupe Garcia-Llatas; Reyes Barberá; Antonio Cilla. Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines. International Journal of Food Sciences and Nutrition 2018, 70, 323 -334.
AMA StyleAndrea Alvarez-Sala, Alessandro Attanzio, Luisa Tesoriere, Guadalupe Garcia-Llatas, Reyes Barberá, Antonio Cilla. Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines. International Journal of Food Sciences and Nutrition. 2018; 70 (3):323-334.
Chicago/Turabian StyleAndrea Alvarez-Sala; Alessandro Attanzio; Luisa Tesoriere; Guadalupe Garcia-Llatas; Reyes Barberá; Antonio Cilla. 2018. "Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines." International Journal of Food Sciences and Nutrition 70, no. 3: 323-334.
Alessandro Attanzio; Luisa Tesoriere; Mahesha Manjunatha Poojary; Antonio Cilla. Fruit and Vegetable Derived Waste as a Sustainable Alternative Source of Nutraceutical Compounds. Journal of Food Quality 2018, 2018, 1 -2.
AMA StyleAlessandro Attanzio, Luisa Tesoriere, Mahesha Manjunatha Poojary, Antonio Cilla. Fruit and Vegetable Derived Waste as a Sustainable Alternative Source of Nutraceutical Compounds. Journal of Food Quality. 2018; 2018 ():1-2.
Chicago/Turabian StyleAlessandro Attanzio; Luisa Tesoriere; Mahesha Manjunatha Poojary; Antonio Cilla. 2018. "Fruit and Vegetable Derived Waste as a Sustainable Alternative Source of Nutraceutical Compounds." Journal of Food Quality 2018, no. : 1-2.
Positive impact on serum lipids and cytokines after functional beverage's intake by postmenopausal women.
Andrea Alvarez-Sala; Virginia Blanco-Morales; Antonio Cilla; Ramona Ángeles Silvestre; Elena Hernández-Álvarez; Fernando Granado-Lorencio; Reyes Barberá; Guadalupe Garcia-Llatas. A positive impact on the serum lipid profile and cytokines after the consumption of a plant sterol-enriched beverage with a milk fat globule membrane: a clinical study. Food & Function 2018, 9, 5209 -5219.
AMA StyleAndrea Alvarez-Sala, Virginia Blanco-Morales, Antonio Cilla, Ramona Ángeles Silvestre, Elena Hernández-Álvarez, Fernando Granado-Lorencio, Reyes Barberá, Guadalupe Garcia-Llatas. A positive impact on the serum lipid profile and cytokines after the consumption of a plant sterol-enriched beverage with a milk fat globule membrane: a clinical study. Food & Function. 2018; 9 (10):5209-5219.
Chicago/Turabian StyleAndrea Alvarez-Sala; Virginia Blanco-Morales; Antonio Cilla; Ramona Ángeles Silvestre; Elena Hernández-Álvarez; Fernando Granado-Lorencio; Reyes Barberá; Guadalupe Garcia-Llatas. 2018. "A positive impact on the serum lipid profile and cytokines after the consumption of a plant sterol-enriched beverage with a milk fat globule membrane: a clinical study." Food & Function 9, no. 10: 5209-5219.
Maria Elisa Caetano-Silva; Antonio Cilla; Maria Teresa Bertoldo-Pacheco; Flavia Maria Netto; Amparo Alegría. Evaluation of in vitro iron bioavailability in free form and as whey peptide-iron complexes. Journal of Food Composition and Analysis 2018, 68, 95 -100.
AMA StyleMaria Elisa Caetano-Silva, Antonio Cilla, Maria Teresa Bertoldo-Pacheco, Flavia Maria Netto, Amparo Alegría. Evaluation of in vitro iron bioavailability in free form and as whey peptide-iron complexes. Journal of Food Composition and Analysis. 2018; 68 ():95-100.
Chicago/Turabian StyleMaria Elisa Caetano-Silva; Antonio Cilla; Maria Teresa Bertoldo-Pacheco; Flavia Maria Netto; Amparo Alegría. 2018. "Evaluation of in vitro iron bioavailability in free form and as whey peptide-iron complexes." Journal of Food Composition and Analysis 68, no. : 95-100.