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The Mediterranean diet (MD) has been sponsored worldwide as a healthy and sustainable diet. Our aim was to update and compare MD adherence and food choices across several Southern European countries: Spain (SP), Portugal (PT), Italy (IT), Greece (GR), and Cyprus (CY) (MED, Mediterranean), and Bulgaria (BG) and the Republic of North Macedonia (NMK) (non-MED, non-Mediterranean). Participants (N = 3145, ≥18 y) completed a survey (MeDiWeB) with sociodemographic, anthropometric, and food questions (14-item Mediterranean Diet Adherence Screener, 14-MEDAS). The MED and non-MED populations showed moderate (7.08 ± 1.96) and weak (5.58 ± 1.82) MD adherence, respectively, with significant yet small differences across countries (SP > PT > GR > IT > CY > BG > NMK, p-value < 0.001). The MED participants scored higher than the non-MED ones for most of the Mediterranean-typical foods, with the greatest differences found for olive oil (OO) and white meat preference. In most countries, ≥70% of the participants reported quantities of red meat, butter, sweet drinks, and desserts below the recommended cutoff points, whereas <50% achieved the targets for plant-based foods, OO, fish, and wine. Being a woman and increasing age were associated with superior adherence (p-value < 0.001), but differences were rather small. Our results suggest that the campaigns carried out to support and reinforce the MD and to promote plant-based foods have limited success across Southern Europe, and that more hard-hitting strategies are needed.
Stefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients 2021, 13, 1432 .
AMA StyleStefano Quarta, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Rui Jorge, Vanda Andrade, Elena Philippou, Constantinos Zisimou, Viktorija Maksimova, Katarina Smilkov, Darinka Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Deligiannidou, Christos Kontogiorgis, Julio Sánchez-Meca, Paula Pinto, María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients. 2021; 13 (5):1432.
Chicago/Turabian StyleStefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. 2021. "Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations?" Nutrients 13, no. 5: 1432.
The Mediterranean diet (MD) and other lifestyle characteristics have been associated with well-being, a broad multiparameter concept that includes individual’s subjective assessment of their own well-being (SWB). Some studies have suggested that diet influences SWB, thus, this work aimed to add novel information on the association of MD and SWB in a sample of Portuguese adults. Data on sociodemographic, economic, lifestyle, diet, and SWB were collected through a self-filled online questionnaire. MD adherence was assessed by the Mediterranean Diet Adherence Screener (MEDAS) score ]. Results showed a moderate adherence to the MD in 490 Portuguese adults (mean MEDAS of 7.4 ± 2.1). A higher MD adherence was found to be significantly positively associated with women, employed individuals, a higher number of meals per day, and those with frequent contact with nature (p-value < 0.0025, using Bonferroni adjustment). As a novelty, this study divided the participants into low SWB, medium SWB, and medium to high SWB profiles (3.9 ± 1.0; 6.2 ± 1.0; 8.2 ± 1.3, respectively; p-value < 0.05), which reported significantly increasing MEDAS scores (6.5 ± 2.1; 7.3 ± 2.1; 7.8 ± 1.9; respectively, p-value < 0.05).
Vanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients 2020, 12, 3837 .
AMA StyleVanda Andrade, Rui Jorge, María-Teresa García-Conesa, Elena Philippou, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients. 2020; 12 (12):3837.
Chicago/Turabian StyleVanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults." Nutrients 12, no. 12: 3837.
This study provides comprehensive validation of the 14-item Mediterranean Diet Adherence Screener (14-MEDAS) in an adult population from Greece (GR), Portugal (PT), Italy (IT), Spain (SP), Cyprus (CY), Republic of North Macedonia (NMK), and Bulgaria (BG). A moderate association between the 14-MEDAS and the reference food diary was estimated for the entire population (Pearson r = 0.573, p-value < 0.001; Intraclass Correlation Coefficient (ICC) = 0.692, p-value < 0.001) with the strongest correlation found in GR, followed by PT, IT, SP, and CY. These results were supported by kappa statistics in GR, PT, IT, and SP with ≥50% of food items exhibiting a fair or better agreement. Bland–Altman analyses showed an overestimation of the 14-MEDAS score in the whole population (0.79 ± 1.81, 95%Confidence Interval (CI) 0.61, 0.96), but this value was variable across countries, with GR, NMK, and BG exhibiting the lowest bias. Taking all analyses together, the validation achieved slightly better results in the Mediterranean countries but a definitive validation ranking order was not evident. Considering growing evidence of the shift from Mediterranean Diet (MD) adherence and of the importance of culture in making food choices it is crucial that we further improve validation protocols with specific applications to compare MD adherence across countries.
María-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients 2020, 12, 2960 .
AMA StyleMaría-Teresa García-Conesa, Elena Philippou, Christos Pafilas, Marika Massaro, Stefano Quarta, Vanda Andrade, Rui Jorge, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients. 2020; 12 (10):2960.
Chicago/Turabian StyleMaría-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region." Nutrients 12, no. 10: 2960.
Food phytochemicals are increasingly considered to play a key role in the cardiometabolic health effects of plant foods. However, the heterogeneity in responsiveness to their intake frequently observed in clinical trials can hinder the beneficial effects of these compounds in specific subpopulations. A range of factors, including genetic background, gut microbiota, age, sex and health status, could be involved in these interindividual variations; however, the current knowledge is limited and fragmented. The European network, European Cooperation in Science and Technology (COST)-POSITIVe, has analysed, in a systematic way, existing knowledge with the aim to better understand the factors responsible for the interindividual variation in response to the consumption of the major families of plant food bioactives, regarding their bioavailability and bioefficacy. If differences in bioavailability, likely reflecting differences in human subjects’ genetics or in gut microbiota composition and functionality, are believed to underpin much of the interindividual variability, the key molecular determinants or microbial species remain to be identified. The systematic analysis of published studies conducted to assess the interindividual variation in biomarkers of cardiometabolic risk suggested some factors (such as adiposity and health status) as involved in between-subject variation. However, the contribution of these factors is not demonstrated consistently across the different compounds and biological outcomes and would deserve further investigations. The findings of the network clearly highlight that the human subjects’ intervention studies published so far are not adequate to investigate the relevant determinants of the absorption/metabolism and biological responsiveness. They also emphasise the need for a new generation of intervention studies designed to capture this interindividual variation.
Christine Morand; Baukje De Roos; Maria Teresa Garcia-Conesa; Eileen R. Gibney; Rikard Landberg; Claudine Manach; Dragan Milenkovic; Ana Rodriguez-Mateos; Tom Van De Wiele; Francisco Tomas-Barberan. Why interindividual variation in response to consumption of plant food bioactives matters for future personalised nutrition. Proceedings of the Nutrition Society 2020, 79, 225 -235.
AMA StyleChristine Morand, Baukje De Roos, Maria Teresa Garcia-Conesa, Eileen R. Gibney, Rikard Landberg, Claudine Manach, Dragan Milenkovic, Ana Rodriguez-Mateos, Tom Van De Wiele, Francisco Tomas-Barberan. Why interindividual variation in response to consumption of plant food bioactives matters for future personalised nutrition. Proceedings of the Nutrition Society. 2020; 79 (2):225-235.
Chicago/Turabian StyleChristine Morand; Baukje De Roos; Maria Teresa Garcia-Conesa; Eileen R. Gibney; Rikard Landberg; Claudine Manach; Dragan Milenkovic; Ana Rodriguez-Mateos; Tom Van De Wiele; Francisco Tomas-Barberan. 2020. "Why interindividual variation in response to consumption of plant food bioactives matters for future personalised nutrition." Proceedings of the Nutrition Society 79, no. 2: 225-235.
Polyphenols are a class of well-known bioactive compounds widely distributed in the plant kingdom and abundant in plant foods and derived food products [...].
María-Teresa García-Conesa; Mar Larrosa. Polyphenol-Rich Foods for Human Health and Disease. Nutrients 2020, 12, 400 .
AMA StyleMaría-Teresa García-Conesa, Mar Larrosa. Polyphenol-Rich Foods for Human Health and Disease. Nutrients. 2020; 12 (2):400.
Chicago/Turabian StyleMaría-Teresa García-Conesa; Mar Larrosa. 2020. "Polyphenol-Rich Foods for Human Health and Disease." Nutrients 12, no. 2: 400.
Background A healthy diet and optimal lifestyle choices are amongst the most important actions for the prevention of cardiometabolic diseases. Despite this, it appears difficult to convince consumers to select more nutritious foods. Furthermore, the development and production of healthier foods do not always lead to economic profits for the agro-food sector. Most dietary recommendations for the general population represent a “one-size-fits-all approach” which does not necessarily ensure that everyone has adequate exposure to health-promoting constituents of foods. Indeed, we now know that individuals show a high variability in responses when exposed to specific nutrients, foods, or diets. Purpose This review aims to highlight our current understanding of inter-individual variability in response to dietary bioactives, based on the integration of findings of the COST Action POSITIVe. We also evaluate opportunities for translation of scientific knowledge on inter-individual variability in response to dietary bioactives, once it becomes available, into practical applications for stakeholders, such as the agro-food industry. The potential impact from such applications will form an important impetus for the food industry to develop and market new high quality and healthy foods for specific groups of consumers in the future. This may contribute to a decrease in the burden of diet-related chronic diseases.
Baukje De Roos; Anna-Marja Aura; Maria Bronze; Aedin Cassidy; María-Teresa Garcia Conesa; Eileen R. Gibney; Arno Greyling; Jim Kaput; Zohar Kerem; Nada Knežević; Paul Kroon; Rikard Landberg; Claudine Manach; Dragan Milenkovic; Ana Rodriguez-Mateos; Francisco A. Tomás-Barberán; Tom Van De Wiele; Christine Morand. Targeting the delivery of dietary plant bioactives to those who would benefit most: from science to practical applications. European Journal of Nutrition 2019, 58, 65 -73.
AMA StyleBaukje De Roos, Anna-Marja Aura, Maria Bronze, Aedin Cassidy, María-Teresa Garcia Conesa, Eileen R. Gibney, Arno Greyling, Jim Kaput, Zohar Kerem, Nada Knežević, Paul Kroon, Rikard Landberg, Claudine Manach, Dragan Milenkovic, Ana Rodriguez-Mateos, Francisco A. Tomás-Barberán, Tom Van De Wiele, Christine Morand. Targeting the delivery of dietary plant bioactives to those who would benefit most: from science to practical applications. European Journal of Nutrition. 2019; 58 (2):65-73.
Chicago/Turabian StyleBaukje De Roos; Anna-Marja Aura; Maria Bronze; Aedin Cassidy; María-Teresa Garcia Conesa; Eileen R. Gibney; Arno Greyling; Jim Kaput; Zohar Kerem; Nada Knežević; Paul Kroon; Rikard Landberg; Claudine Manach; Dragan Milenkovic; Ana Rodriguez-Mateos; Francisco A. Tomás-Barberán; Tom Van De Wiele; Christine Morand. 2019. "Targeting the delivery of dietary plant bioactives to those who would benefit most: from science to practical applications." European Journal of Nutrition 58, no. 2: 65-73.
The obligate intracellular bacterium Chlamydia abortus causes abortion and constitutes a worldwide threat for livestock. Plant-derived flavonoids have antimicrobial effects against veterinary and human pathogens and may be of help in the fight against C. abortus. The anti-infective efficacy against C. abortus of the flavonoids apigenin and naringenin, and of four of their derived metabolites was explored using an in vitro model of ovine trophoblast cells. Overall, the anti-infective effectiveness was apigenin > naringenin > benzoic acid > 3-(4-hydroxyphenyl) propionic acid ~ 4-hydroxyphenyl acetic acid ~ 4-hydroxybenzoic. At the lowest concentration tested (10 μM), apigenin, naringenin and benzoic acid inhibited the formation of C. abortus inclusions by 80%, 67%, and 39%, respectively. The cytotoxicity and anti-proliferative effects on the trophoblast host cells also differed greatly between the tested compounds. Our findings suggest that flavonoids may be of therapeutic value against C. abortus infection but metabolic conversion has a substantial and variable effect on their anti-chlamydial activity. Our results also support the notion that the mechanisms of anti-infective action may involve combined effects of the compounds against the host cells and the bacteria.
Laura Del Rio; Jesús Salinas; Antonio J. Buendía; María-Teresa García-Conesa. The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line. Research in Veterinary Science 2019, 126, 199 -206.
AMA StyleLaura Del Rio, Jesús Salinas, Antonio J. Buendía, María-Teresa García-Conesa. The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line. Research in Veterinary Science. 2019; 126 ():199-206.
Chicago/Turabian StyleLaura Del Rio; Jesús Salinas; Antonio J. Buendía; María-Teresa García-Conesa. 2019. "The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line." Research in Veterinary Science 126, no. : 199-206.
Chokeberry polyphenols have been suggested to reduce cholesterol and blood pressure and thus protect against cardiovascular diseases (CVD), but the evidence in humans is limited and inconsistent. This randomized double-blinded three-parallel groups trial investigated the changes in various anthropometric and clinical biomarkers, and in plasma phospholipids fatty acids (PPFA) in volunteers at cardiovascular risk after a four-week intervention with 100 mL/day of (1) chokeberry juice with a high-dose of polyphenols (1177.11 mg gallic acid equivalents, GAE); (2) chokeberry juice with a low-dose of polyphenols (294.28 mg GAE) and; (3) a nutritionally matched polyphenol-free placebo drink. Our results indicate that the intake of chokeberry juice containing either the low or the high dose of polyphenols cannot be linked with a reduction in total- and low-density lipoprotein (LDL)cholesterol or in systolic (SBP) and diastolic (DBP) blood pressure in comparison with the consumption of the placebo drink. However, we found evidence of moderate changes in the PPFA, i.e., increased saturated fatty acids (SFA), mostly palmitic acid, and reduced n-6 polyunsaturated fatty acids (PUFA), principally linoleic acid (LA) with the intake of chokeberry against the placebo. These effects may be associated with the polyphenols but we could not differentiate a clear dose-response effect. Further research is still needed to elucidate the contribution of the polyphenolic fraction to the potential cardiovascular effects of the chokeberry and to build up the evidence of its potential benefit via the modulation of PPFA composition.
Biljana Pokimica; María-Teresa García-Conesa; Manja Zec; Jasmina Debeljak-Martačić; Slavica Ranković; Nevena Vidović; Gordana Petrović-Oggiano; Aleksandra Konić-Ristić; Maria Glibetić. Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. Nutrients 2019, 11, 850 .
AMA StyleBiljana Pokimica, María-Teresa García-Conesa, Manja Zec, Jasmina Debeljak-Martačić, Slavica Ranković, Nevena Vidović, Gordana Petrović-Oggiano, Aleksandra Konić-Ristić, Maria Glibetić. Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk. Nutrients. 2019; 11 (4):850.
Chicago/Turabian StyleBiljana Pokimica; María-Teresa García-Conesa; Manja Zec; Jasmina Debeljak-Martačić; Slavica Ranković; Nevena Vidović; Gordana Petrović-Oggiano; Aleksandra Konić-Ristić; Maria Glibetić. 2019. "Chokeberry Juice Containing Polyphenols Does Not Affect Cholesterol or Blood Pressure but Modifies the Composition of Plasma Phospholipids Fatty Acids in Individuals at Cardiovascular Risk." Nutrients 11, no. 4: 850.
Probiotics constitute an attractive alternative in the battle against microbial infections. Oral administration of certain strains of lactobacilli isolated from human milk has resulted in an effective reduction of the bacterial load as well as an improvement of the mastitis-associated symptoms. Nevertheless, little is yet known about the potential molecular mechanisms and specific targets implicated in these effects. Transcriptomic profiling has been used to search for disease-associated and therapy-responsive molecules in different disorders and experimental models. We have applied for the first time a gene expression-based molecular approach to explore for potential targets responsive to intervention with a probiotic in: (i) breast milk somatic cells (n = 17) and (ii) blood leukocytes (n = 19). Women with mastitis ingested a new strain of lactobacilli, Lactobacillus salivarius PS2 (3 × capsules per day, each capsule contained ~9.5 log10 CFU) for 21 days. We applied Affymetrix microarrays and Taqman one-step quantitative reverse transcription PCR (RT-qPCR) to analyze and compare gene expression changes between samples pre- and post-treatment. Our results substantiate the involvement of inflammatory and cell-growth related pathways and genes in the breast milk somatic cells following the intake of L. salivarius PS2. Individual analyses of selected genes: (1) supported the upregulation of STC1 and IL19 and the downregulation of PLAUR and IFNGR1 in the somatic cells of the patients as potential targets responsive to the probiotic, (2) detected a lack of a relationship between the gene expression responses in the two types of cells, and (3) evidenced a substantial interindividual variability in the gene expression changes in both types of cells. Our study provides an insight into the essentiality of incorporating the study of tissue-specific interindividual molecular responsivity into future clinical intervention trials to further understand the complexity of human gene expression responses to therapy and the potentiality of selecting appropriate responsive targets.
Javier De Andrés; Esther Jiménez; Irene Espinosa-Martos; Juan Rodríguez; María-Teresa García-Conesa. An Exploratory Search for Potential Molecular Targets Responsive to the Probiotic Lactobacillus salivarius PS2 in Women With Mastitis: Gene Expression Profiling vs. Interindividual Variability. Frontiers in Microbiology 2018, 9, 1 .
AMA StyleJavier De Andrés, Esther Jiménez, Irene Espinosa-Martos, Juan Rodríguez, María-Teresa García-Conesa. An Exploratory Search for Potential Molecular Targets Responsive to the Probiotic Lactobacillus salivarius PS2 in Women With Mastitis: Gene Expression Profiling vs. Interindividual Variability. Frontiers in Microbiology. 2018; 9 ():1.
Chicago/Turabian StyleJavier De Andrés; Esther Jiménez; Irene Espinosa-Martos; Juan Rodríguez; María-Teresa García-Conesa. 2018. "An Exploratory Search for Potential Molecular Targets Responsive to the Probiotic Lactobacillus salivarius PS2 in Women With Mastitis: Gene Expression Profiling vs. Interindividual Variability." Frontiers in Microbiology 9, no. : 1.
Pre-clinical cell and animal nutrigenomic studies have long suggested the modulation of the transcription of multiple gene targets in cells and tissues as a potential molecular mechanism of action underlying the beneficial effects attributed to plant-derived bioactive compounds. To try to demonstrate these molecular effects in humans, a considerable number of clinical trials have now explored the changes in the expression levels of selected genes in various human cell and tissue samples following intervention with different dietary sources of bioactive compounds. In this review, we have compiled a total of 75 human studies exploring gene expression changes using quantitative reverse transcription PCR (RT-qPCR). We have critically appraised the study design and methodology used as well as the gene expression results reported. We herein pinpoint some of the main drawbacks and gaps in the experimental strategies applied, as well as the high interindividual variability of the results and the limited evidence supporting some of the investigated genes as potential responsive targets. We reinforce the need to apply normalized procedures and follow well-established methodological guidelines in future studies in order to achieve improved and reliable results that would allow for more relevant and biologically meaningful results.
Biljana Pokimica; María-Teresa García-Conesa. Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. Nutrients 2018, 10, 807 .
AMA StyleBiljana Pokimica, María-Teresa García-Conesa. Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. Nutrients. 2018; 10 (7):807.
Chicago/Turabian StyleBiljana Pokimica; María-Teresa García-Conesa. 2018. "Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies." Nutrients 10, no. 7: 807.
Understanding interindividual variability in response to dietary polyphenols remains essential to elucidate their effects on cardiometabolic disease development. A meta-analysis of 128 randomized clinical trials was conducted to investigate the effects of berries and red grapes/wine as sources of anthocyanins and of nuts and pomegranate as sources of ellagitannins on a range of cardiometabolic risk biomarkers. The potential influence of various demographic and lifestyle factors on the variability in the response to these products were explored. Both anthocyanin- and ellagitannin-containing products reduced total-cholesterol with nuts and berries yielding more significant effects than pomegranate and grapes. Blood pressure was significantly reduced by the two main sources of anthocyanins, berries and red grapes/wine, whereas waist circumference, LDL-cholesterol, triglycerides, and glucose were most significantly lowered by the ellagitannin-products, particularly nuts. Additionally, we found an indication of a small increase in HDL-cholesterol most significant with nuts and, in flow-mediated dilation by nuts and berries. Most of these effects were detected in obese/overweight people but we found limited or non-evidence in normoweight individuals or of the influence of sex or smoking status. The effects of other factors, i.e., habitual diet, health status or country where the study was conducted, were inconsistent and require further investigation.
María-Teresa García-Conesa; Karen Chambers; Emilie Combet; Paula Pinto; Mar Garcia-Aloy; Cristina Andrés-Lacueva; Sonia De Pascual-Teresa; Pedro Mena; Aleksandra Konic Ristic; Wendy J. Hollands; Paul A. Kroon; Ana Rodríguez-Mateos; Geoffrey Istas; Christos A. Kontogiorgis; Dilip K. Rai; Eileen R. Gibney; Christine Morand; Juan Carlos Espín; Antonio González-Sarrías. Meta-Analysis of the Effects of Foods and Derived Products Containing Ellagitannins and Anthocyanins on Cardiometabolic Biomarkers: Analysis of Factors Influencing Variability of the Individual Responses. International Journal of Molecular Sciences 2018, 19, 694 .
AMA StyleMaría-Teresa García-Conesa, Karen Chambers, Emilie Combet, Paula Pinto, Mar Garcia-Aloy, Cristina Andrés-Lacueva, Sonia De Pascual-Teresa, Pedro Mena, Aleksandra Konic Ristic, Wendy J. Hollands, Paul A. Kroon, Ana Rodríguez-Mateos, Geoffrey Istas, Christos A. Kontogiorgis, Dilip K. Rai, Eileen R. Gibney, Christine Morand, Juan Carlos Espín, Antonio González-Sarrías. Meta-Analysis of the Effects of Foods and Derived Products Containing Ellagitannins and Anthocyanins on Cardiometabolic Biomarkers: Analysis of Factors Influencing Variability of the Individual Responses. International Journal of Molecular Sciences. 2018; 19 (3):694.
Chicago/Turabian StyleMaría-Teresa García-Conesa; Karen Chambers; Emilie Combet; Paula Pinto; Mar Garcia-Aloy; Cristina Andrés-Lacueva; Sonia De Pascual-Teresa; Pedro Mena; Aleksandra Konic Ristic; Wendy J. Hollands; Paul A. Kroon; Ana Rodríguez-Mateos; Geoffrey Istas; Christos A. Kontogiorgis; Dilip K. Rai; Eileen R. Gibney; Christine Morand; Juan Carlos Espín; Antonio González-Sarrías. 2018. "Meta-Analysis of the Effects of Foods and Derived Products Containing Ellagitannins and Anthocyanins on Cardiometabolic Biomarkers: Analysis of Factors Influencing Variability of the Individual Responses." International Journal of Molecular Sciences 19, no. 3: 694.
Several randomized controlled trials (RCTs) and meta-analyses support the benefits of flavanols on cardiometabolic health, but the factors affecting variability in the responses to these compounds have not been properly assessed. The objectives of this meta-analysis were to systematically collect the RCTs-based-evidence of the effects of flavanol-containing tea, cocoa and apple products on selected biomarkers of cardiometabolic risk and to explore the influence of various factors on the variability in the responses to the consumption of these products. A total of 120 RCTs were selected. Despite a high heterogeneity, the intake of the flavanol-containing products was associated using a random model with changes (reported as standardized difference in means (SDM)) in body mass index (−0.15, p < 0.001), waist circumference (−0.29, p < 0.001), total-cholesterol (−0.21, p < 0.001), LDL-cholesterol (−0.23, p < 0.001), and triacylglycerides (−0.11, p = 0.027), and with an increase of HDL-cholesterol (0.15, p = 0.005). Through subgroup analyses, we showed the influence of baseline-BMI, sex, source/form of administration, medication and country of investigation on some of the outcome measures and suggest that flavanols may be more effective in specific subgroups such as those with a BMI ≥ 25.0 kg/m2, non-medicated individuals or by specifically using tea products. This meta-analysis provides the first robust evidence of the effects induced by the consumption of flavanol-containing tea, cocoa and apple products on weight and lipid biomarkers and shows the influence of various factors that can affect their bioefficacy in humans. Of note, some of these effects are quantitatively comparable to those produced by drugs, life-style changes or other natural products. Further, RCTs in well-characterized populations are required to fully comprehend the factors affecting inter-individual responses to flavanol and thereby improve flavanols efficacy in the prevention of cardiometabolic disorders.
Antonio González-Sarrías; Emilie Combet; Paula Pinto; Pedro Mena; Margherita Dall’Asta; Mar Garcia-Aloy; Ana Rodríguez-Mateos; Eileen R. Gibney; Julie Dumont; Marika Massaro; Julio Sánchez-Meca; Christine Morand; María-Teresa García-Conesa. A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy. Nutrients 2017, 9, 746 .
AMA StyleAntonio González-Sarrías, Emilie Combet, Paula Pinto, Pedro Mena, Margherita Dall’Asta, Mar Garcia-Aloy, Ana Rodríguez-Mateos, Eileen R. Gibney, Julie Dumont, Marika Massaro, Julio Sánchez-Meca, Christine Morand, María-Teresa García-Conesa. A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy. Nutrients. 2017; 9 (7):746.
Chicago/Turabian StyleAntonio González-Sarrías; Emilie Combet; Paula Pinto; Pedro Mena; Margherita Dall’Asta; Mar Garcia-Aloy; Ana Rodríguez-Mateos; Eileen R. Gibney; Julie Dumont; Marika Massaro; Julio Sánchez-Meca; Christine Morand; María-Teresa García-Conesa. 2017. "A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy." Nutrients 9, no. 7: 746.
The clinical evidence of dietary polyphenols as colorectal cancer (CRC) chemopreventive compounds is very weak. Verification in humans of tissue-specific molecular regulation by the intake of polyphenols requires complex clinical trials that allow for the procurement of sufficient pre- and postsupplementation tissue samples. Ellagitannins (ETs), ellagic acid (EA) and their gut microbiota-derived metabolites, the urolithins, modify gene expression in colon normal and cancer cultured cells. We conducted here the first clinical trial with 35 CRC patients daily supplemented with 900 mg of an ET-containing pomegranate extract (PE) and evaluated the expression of various CRC-related genes in normal and cancerous colon tissues before (biopsies) and after (surgical specimens) 5–35 days of supplementation. Tissues were also obtained from 10 control patients (no supplementation) that confirmed a large, gene- and tissue-specific interindividual variability and impact of the experimental protocol on gene expression, with some genes induced (MYC, CD44, CDKN1A, CTNNB1), some repressed (CASP3) and others not affected (KRAS). Despite these issues, the consumption of the PE was significantly associated with a counterbalance effect in the expression of CD44, CTNNB1, CDKN1A, EGFR and TYMs, suggesting that the intake of this PE modulated the impact of the protocol on gene expression in a gene- and tissue-specific manner. These effects were not associated with the individuals' capacity to produce specific urolithins (i.e., metabotypes) or the levels of urolithins and EA in the colon tissues and did not reproduce in vitro effects evidencing the difficulty of demonstrating in vivo the in vitro results.
María A. Nuñez-Sánchez; Antonio González-Sarrías; Rocio Garcia Villalba; Tamara Monedero-Saiz; Noelia V. García-Talavera; María B. Gómez-Sánchez; Carmen Sánchez-Álvarez; Ana M. García-Albert; Francisco J. Rodríguez-Gil; Miguel Ruiz-Marín; Francisco A. Pastor-Quirante; Francisco Martínez-Díaz; Francisco Tomas-Barberan; Juan Carlos Espín; María-Teresa García-Conesa. Gene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical trial. The Journal of Nutritional Biochemistry 2017, 42, 126 -133.
AMA StyleMaría A. Nuñez-Sánchez, Antonio González-Sarrías, Rocio Garcia Villalba, Tamara Monedero-Saiz, Noelia V. García-Talavera, María B. Gómez-Sánchez, Carmen Sánchez-Álvarez, Ana M. García-Albert, Francisco J. Rodríguez-Gil, Miguel Ruiz-Marín, Francisco A. Pastor-Quirante, Francisco Martínez-Díaz, Francisco Tomas-Barberan, Juan Carlos Espín, María-Teresa García-Conesa. Gene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical trial. The Journal of Nutritional Biochemistry. 2017; 42 ():126-133.
Chicago/Turabian StyleMaría A. Nuñez-Sánchez; Antonio González-Sarrías; Rocio Garcia Villalba; Tamara Monedero-Saiz; Noelia V. García-Talavera; María B. Gómez-Sánchez; Carmen Sánchez-Álvarez; Ana M. García-Albert; Francisco J. Rodríguez-Gil; Miguel Ruiz-Marín; Francisco A. Pastor-Quirante; Francisco Martínez-Díaz; Francisco Tomas-Barberan; Juan Carlos Espín; María-Teresa García-Conesa. 2017. "Gene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical trial." The Journal of Nutritional Biochemistry 42, no. : 126-133.
Bioactive compounds in plant-based foods have health properties that contribute to the prevention of age-related chronic diseases, particularly cardiometabolic disorders. Conclusive proof and understanding of these benefits in humans is essential in order to provide effective dietary recommendations but, so far, the evidence obtained from human intervention trials is limited and contradictory. This is partly due to differences between individuals in the absorption, distribution, metabolism and excretion of bioactive compounds, as well as to heterogeneity in their biological response regarding cardiometabolic health outcomes. Identifying the main factors underlying inter-individual differences, as well as developing new and innovative methodologies to account for such variability constitute an overarching goal to ultimately optimize the beneficial health effects of plant food bioactives for each and every one of us. In this respect, this position paper from the COST Action FA1403-POSITIVe examines the main factors likely to affect the individual responses to consumption of plant food bioactives and presents perspectives for assessment and consideration of inter-individual variability.
Claudine Manach; Dragan Milenkovic; Tom Van De Wiele; Ana Rodriguez-Mateos; Baukje De Roos; Maria Teresa Garcia Conesa; Rikard Landberg; Eileen R. Gibney; Marina Heinonen; Francisco Tomas-Barberan; Christine Morand. Addressing the inter-individual variation in response to consumption of plant food bioactives: Towards a better understanding of their role in healthy aging and cardiometabolic risk reduction. Molecular Nutrition & Food Research 2016, 61, 1 .
AMA StyleClaudine Manach, Dragan Milenkovic, Tom Van De Wiele, Ana Rodriguez-Mateos, Baukje De Roos, Maria Teresa Garcia Conesa, Rikard Landberg, Eileen R. Gibney, Marina Heinonen, Francisco Tomas-Barberan, Christine Morand. Addressing the inter-individual variation in response to consumption of plant food bioactives: Towards a better understanding of their role in healthy aging and cardiometabolic risk reduction. Molecular Nutrition & Food Research. 2016; 61 (6):1.
Chicago/Turabian StyleClaudine Manach; Dragan Milenkovic; Tom Van De Wiele; Ana Rodriguez-Mateos; Baukje De Roos; Maria Teresa Garcia Conesa; Rikard Landberg; Eileen R. Gibney; Marina Heinonen; Francisco Tomas-Barberan; Christine Morand. 2016. "Addressing the inter-individual variation in response to consumption of plant food bioactives: Towards a better understanding of their role in healthy aging and cardiometabolic risk reduction." Molecular Nutrition & Food Research 61, no. 6: 1.
Urolithins are dibenzo[b,d]pyran‐6‐one derivatives that are produced by the human gut microbiota from ellagitannins and ellagic acid (EA). These metabolites are much better absorbed than their precursors and have been suggested to be responsible for the health effects attributed to ellagitannins and EA that occur in food products as berries and nuts. In the present review, the role and potential of urolithins in human health are critically reviewed, and a perspective of the research approach needed to demonstrate these health effects is presented, based on the existing knowledge. The analytical methods available for urolithin analysis, their occurrence in different tissues and biological fluids, and their metabolism by human gut microbiota are considered. In addition, the interindividual variability observed for the production of urolithins (metabotypes) and its relationship with health status and dysbiosis are also reviewed. The potential mechanisms of action of urolithins are also critically discussed, paying attention to the concentration and the type of metabolites used in the in vitro and in vivo assays and the physiological significance of the results obtained. The gut microbiota metabolism of EA to urolithins and that of daidzein to equol, their individual variations, and the effects on health are also compared.
Francisco A. Tomás-Barberán; Antonio González-Sarrías; Rocio Garcia Villalba; Maria Angeles Nuñez Sanchez; Maria Victoria Selma; Maria Teresa Garcia Conesa; Juan Carlos Espín. Urolithins, the rescue of “old” metabolites to understand a “new” concept: Metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status. Molecular Nutrition & Food Research 2016, 61, 1 .
AMA StyleFrancisco A. Tomás-Barberán, Antonio González-Sarrías, Rocio Garcia Villalba, Maria Angeles Nuñez Sanchez, Maria Victoria Selma, Maria Teresa Garcia Conesa, Juan Carlos Espín. Urolithins, the rescue of “old” metabolites to understand a “new” concept: Metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status. Molecular Nutrition & Food Research. 2016; 61 (1):1.
Chicago/Turabian StyleFrancisco A. Tomás-Barberán; Antonio González-Sarrías; Rocio Garcia Villalba; Maria Angeles Nuñez Sanchez; Maria Victoria Selma; Maria Teresa Garcia Conesa; Juan Carlos Espín. 2016. "Urolithins, the rescue of “old” metabolites to understand a “new” concept: Metabotypes as a nexus among phenolic metabolism, microbiota dysbiosis, and host health status." Molecular Nutrition & Food Research 61, no. 1: 1.
Colon cancer stem cells (CSCs) offer a novel paradigm for colorectal cancer (CRC) treatment and dietary polyphenols may contribute to battle these cells. Specifically, polyphenol-derived colon metabolites have the potential to interact with and affect colon CSCs. We herein report the effects against colon CSCs of two mixtures of ellagitannin (ET) metabolites, ellagic acid (EA) and the gut microbiota-derived urolithins (Uro) at concentrations detected in the human colon tissues following the intake of ET-containing products (pomegranate, walnuts). These mixtures reduce phenotypic and molecular features in two models of colon CSCs: Caco-2 cells and primary tumour cells from a patient with CRC. The mixture containing mostly Uro-A (85% Uro-A, 10% Uro-C, 5% EA) was most effective at inhibiting the number and size of colonospheres and aldehyde dehydrogenase activity (ALDH, a marker of chemoresistance) whereas the mixture containing less Uro-A but IsoUro-A and Uro-B (30% Uro-A, 50% IsoUro-A, 10% Uro-B, 5% Uro-C, 5% EA) had some effects on the number and size of colonospheres but not on ALDH. These data support a role for polyphenols metabolites in the control of colon cancer chemoresistance and relapse and encourage the research on the effects of polyphenols against CSCs.
Maria Angeles Nuñez Sanchez; Ankur Karmokar; Antonio González-Sarrías; Rocio Garcia Villalba; Francisco A. Tomás-Barberán; María Teresa García-Conesa; Karen Brown; Juan Carlos Espín. In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer. Food and Chemical Toxicology 2016, 92, 8 -16.
AMA StyleMaria Angeles Nuñez Sanchez, Ankur Karmokar, Antonio González-Sarrías, Rocio Garcia Villalba, Francisco A. Tomás-Barberán, María Teresa García-Conesa, Karen Brown, Juan Carlos Espín. In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer. Food and Chemical Toxicology. 2016; 92 ():8-16.
Chicago/Turabian StyleMaria Angeles Nuñez Sanchez; Ankur Karmokar; Antonio González-Sarrías; Rocio Garcia Villalba; Francisco A. Tomás-Barberán; María Teresa García-Conesa; Karen Brown; Juan Carlos Espín. 2016. "In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer." Food and Chemical Toxicology 92, no. : 8-16.
Ellagitannins, ellagic acid, and the colonic metabolites urolithins (Uros) exhibit anticancer effects against colon cells, but a comprehensive molecular analysis has not been done. Herein, we used a panel of cell lines to first time evaluate the antiproliferative properties and accompanying molecular responses of two ellagitannin metabolites mixtures mimicking the situation in vivo and of each individual metabolite. We examined cell growth, cell cycle, apoptosis, and the expression of related genes and microRNAs (miRs) in a panel of nonmalignant and malignant colon cell lines. Regardless of the composition, the mixed metabolites similarly inhibited proliferation, induced cycle arrest, and apoptosis. All the metabolites contributed to these effects, but Uro-A, isourolithin A, Uro-C, and Uro-D were more potent than Uro-B and ellagic acid. Despite molecular differences between the cell lines, we discerned relevant changes in key cancer markers and corroborated the induction of CDKN1A (cyclin-dependent kinase inhibitor 1A gene (p21, Cip1); encoding p21) as a common step underlying the anticancer properties of Uros. Interestingly, cell-unique downregulation of miR-224 or upregulation of miR-215 was found associated with CDKN1A induction. Physiologically relevant mixtures of Uros exert anticancer effects against colon cancer cells via a common CDKN1A upregulatory mechanism. Other associated molecular responses are however heterogeneous and mostly cell-specific.
Antonio González-Sarrías; Maria Angeles Nuñez Sanchez; João Tomé-Carneiro; Francisco Tomas-Barberan; Maria Teresa Garcia Conesa; Juan Carlos Espín. Comprehensive characterization of the effects of ellagic acid and urolithins on colorectal cancer and key-associated molecular hallmarks: MicroRNA cell specific induction of CDKN1A (p21) as a common mechanism involved. Molecular Nutrition & Food Research 2015, 60, 701 -716.
AMA StyleAntonio González-Sarrías, Maria Angeles Nuñez Sanchez, João Tomé-Carneiro, Francisco Tomas-Barberan, Maria Teresa Garcia Conesa, Juan Carlos Espín. Comprehensive characterization of the effects of ellagic acid and urolithins on colorectal cancer and key-associated molecular hallmarks: MicroRNA cell specific induction of CDKN1A (p21) as a common mechanism involved. Molecular Nutrition & Food Research. 2015; 60 (4):701-716.
Chicago/Turabian StyleAntonio González-Sarrías; Maria Angeles Nuñez Sanchez; João Tomé-Carneiro; Francisco Tomas-Barberan; Maria Teresa Garcia Conesa; Juan Carlos Espín. 2015. "Comprehensive characterization of the effects of ellagic acid and urolithins on colorectal cancer and key-associated molecular hallmarks: MicroRNA cell specific induction of CDKN1A (p21) as a common mechanism involved." Molecular Nutrition & Food Research 60, no. 4: 701-716.
The bark, seeds, fruits and leaves of the genus Fraxinus (Oleaceae) which contain a wide range of phytochemicals, mostly secoiridoid glucosides, have been widely used in folk medicine against a number of ailments, yet little is known about the metabolism and uptake of the major Fraxinus components. The aim of this work was to advance in the knowledge on the bioavailability of the secoiridoids present in a Fraxinus angustifolia Vahl seed/fruit extract using both targeted and untargeted metabolomic analyses. Plasma and urine samples from nine healthy volunteers were taken at specific time intervals following the intake of the extract and analyzed by UPLC-ESI-QTOF. Predicted metabolites such as tyrosol and ligstroside-aglycone glucuronides and sulfates were detected at low intensity. These compounds reached peak plasma levels 2 h after the intake and exhibited high variability among the participants. The ligstroside-aglycone conjugates may be considered as potential biomarkers of the Fraxinus secoiridoids intake. Using the untargeted approach we additionally detected phenolic conjugates identified as ferulic acid and caffeic acid sulfates, as well as hydroxybenzyl and hydroxyphenylacetaldehyde sulfate derivatives which support further metabolism of the secoiridoids by phase I and (or) microbial enzymes. Overall, the results of this study suggest low uptake of intact secoiridoids from a Fraxinus angustifolia Vahl extract in healthy human volunteers and metabolic conversion by esterases, glycosidases, and phase II sulfo- and glucuronosyl transferases to form smaller conjugated derivatives.
Rocío García-Villalba; Francisco A. Tomás-Barberán; Pascale Fança-Berthon; Marc Roller; Pilar Zafrilla; Nicolas Issaly; María-Teresa García-Conesa. Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study. Molecules 2015, 20, 22202 -22219.
AMA StyleRocío García-Villalba, Francisco A. Tomás-Barberán, Pascale Fança-Berthon, Marc Roller, Pilar Zafrilla, Nicolas Issaly, María-Teresa García-Conesa. Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study. Molecules. 2015; 20 (12):22202-22219.
Chicago/Turabian StyleRocío García-Villalba; Francisco A. Tomás-Barberán; Pascale Fança-Berthon; Marc Roller; Pilar Zafrilla; Nicolas Issaly; María-Teresa García-Conesa. 2015. "Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study." Molecules 20, no. 12: 22202-22219.
: MicroRNAs (miRs) are proposed as colorectal cancer (CRC) biomarkers. Pomegranate ellagic acid and their microbiota metabolites urolithins exert anticancer effects in preclinical CRC models, and target normal and malignant colon tissues in CRC patients. Herein, we investigated whether the intake of pomegranate extract (PE) modified miRs expression in surgical colon tissues vs biopsies from CRC patients. : We conducted a randomized, double‐blind, controlled trial. Thirty‐five CRC patients consumed 900 mg PE daily before surgery. Control CRC patients (no PE intake, n = 10) were included. Our results revealed: 1) Significant differences for specific miRs between malignant and normal tissues modifiable by the surgical protocols; 2) opposed trends between ‐5p and ‐3p isomolecules; 3) general induction of miRs attributable to the surgery; 4) moderate modulation of various miRs following the PE intake and 5) no association between tissue urolithins and the observed miRs changes. : PE consumption appears to affect specific colon tissue miRs but surgery critically alters miRs levels hindering the discrimination of significant changes caused by dietary factors and the establishment of genuine differences between malignant and normal tissues as biomarkers. The components responsible for the PE effects and the clinical relevance of these observations deserve further research. This article is protected by copyright. All rights reserved
María A. Nuñez-Sánchez; Alberto Davalos; Antonio González-Sarrías; Patricia Casas-Agustench; Francesco Visioli; Tamara Monedero-Saiz; Noelia V. García-Talavera; María B. Gómez-Sánchez; Carmen Sánchez-Álvarez; Ana M. García-Albert; Francisco J. Rodríguez-Gil; Miguel Ruiz-Marín; Francisco A. Pastor-Quirante; Francisco Martínez-Díaz; Francisco Tomas-Barberan; María Teresa García-Conesa; Juan Carlos Espín. MicroRNAs expression in normal and malignant colon tissues as biomarkers of colorectal cancer and in response to pomegranate extracts consumption: Critical issues to discern between modulatory effects and potential artefacts. Molecular Nutrition & Food Research 2015, 59, 1973 -1986.
AMA StyleMaría A. Nuñez-Sánchez, Alberto Davalos, Antonio González-Sarrías, Patricia Casas-Agustench, Francesco Visioli, Tamara Monedero-Saiz, Noelia V. García-Talavera, María B. Gómez-Sánchez, Carmen Sánchez-Álvarez, Ana M. García-Albert, Francisco J. Rodríguez-Gil, Miguel Ruiz-Marín, Francisco A. Pastor-Quirante, Francisco Martínez-Díaz, Francisco Tomas-Barberan, María Teresa García-Conesa, Juan Carlos Espín. MicroRNAs expression in normal and malignant colon tissues as biomarkers of colorectal cancer and in response to pomegranate extracts consumption: Critical issues to discern between modulatory effects and potential artefacts. Molecular Nutrition & Food Research. 2015; 59 (10):1973-1986.
Chicago/Turabian StyleMaría A. Nuñez-Sánchez; Alberto Davalos; Antonio González-Sarrías; Patricia Casas-Agustench; Francesco Visioli; Tamara Monedero-Saiz; Noelia V. García-Talavera; María B. Gómez-Sánchez; Carmen Sánchez-Álvarez; Ana M. García-Albert; Francisco J. Rodríguez-Gil; Miguel Ruiz-Marín; Francisco A. Pastor-Quirante; Francisco Martínez-Díaz; Francisco Tomas-Barberan; María Teresa García-Conesa; Juan Carlos Espín. 2015. "MicroRNAs expression in normal and malignant colon tissues as biomarkers of colorectal cancer and in response to pomegranate extracts consumption: Critical issues to discern between modulatory effects and potential artefacts." Molecular Nutrition & Food Research 59, no. 10: 1973-1986.
The aim of this review was to critically assess the evidence supporting the metabolic and anti-inflammatory effects attributed to polyphenols and the potential mechanisms of action underlying these effects. The metabolic and anti-inflammatory properties of polyphenols and polyphenol-rich products have been shown mostly in rodents. These compounds appear to share multiple mechanisms of action at different body sites (gastrointestinal tract, microbiota, host organs) and the responsible molecules may be the original plant compounds, the microbial metabolites and (or) the host derived conjugates. Polyphenols may modify digestion and absorption of nutrients, microbiota composition and metabolism, and host tissue metabolic pathways but none of these mechanisms have been fully demonstrated in vivo and thus, more and better designed studies are needed. Furthermore, human clinical trials show inconsistent evidence of the metabolic and inflammation regulatory properties of polyphenols. Some of the principal limitations of these studies as well as recommendations to further progress in the understanding of the metabolic effects and mechanisms of action of polyphenols are discussed.
Maria-Teresa Garcia-Conesa. Dietary Polyphenols against Metabolic Disorders: How Far Have We Progressed in the Understanding of the Molecular Mechanisms of Action of These Compounds? Critical Reviews in Food Science and Nutrition 2015, 00 -00.
AMA StyleMaria-Teresa Garcia-Conesa. Dietary Polyphenols against Metabolic Disorders: How Far Have We Progressed in the Understanding of the Molecular Mechanisms of Action of These Compounds? Critical Reviews in Food Science and Nutrition. 2015; ():00-00.
Chicago/Turabian StyleMaria-Teresa Garcia-Conesa. 2015. "Dietary Polyphenols against Metabolic Disorders: How Far Have We Progressed in the Understanding of the Molecular Mechanisms of Action of These Compounds?" Critical Reviews in Food Science and Nutrition , no. : 00-00.