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Plants are affected by numerous environmental factors that influence their physiological processes and productivity. Early revealing of their action based on measuring spectra of reflected light and calculating reflectance indices is an important stage in the protection of agricultural plants. Photochemical reflectance index (PRI) is a widely used parameter related to photosynthetic changes in plants under action of stressors. We developed a new system for proximal imaging of PRI based on using short pulses of measuring light detected simultaneously in green (530 nm) and yellow (570 nm) spectral bands. The system has several advances compared to those reported in literature. Active light illumination and subtraction of the ambient light allow for PRI measurements without periodic calibrations. Short duration of measuring pulses (18 ms) minimizes their influence on plants. Measurements in two spectral bands operated by separate cameras with aligned fields of visualization allow one to exclude mechanically switchable parts like filter wheels thus minimizing acquisition time and increasing durability of the setup. Absolute values of PRI and light-induced changes in PRI (ΔPRI) in pea leaves and changes of these parameters under action of light with different intensities, water shortage, and heating have been investigated using the developed setup. Changes in ΔPRI are shown to be more robust than the changes in the absolute value of PRI which is in a good agreement with our previous studies. Values of PRI and, especially, ΔPRI are strongly linearly related to the energy-dependent component of the non-photochemical quenching and can be potentially used for estimation of this component. Additionally, we demonstrate that the developed system can also measure fast changes in PRI (hundreds of milliseconds and seconds) under leaf illumination by the pulsed green-yellow measuring light. Thus, the developed system of proximal PRI imaging can be used for PRI measurements (including fast changes in PRI) and estimation of stressors-induced photosynthetic changes.
Vladimir Sukhov; Ekaterina Sukhova; Andrey Khlopkov; Lyubov Yudina; Anastasiia Ryabkova; Alexander Telnykh; Ekaterina Sergeeva; Vladimir Vodeneev; Ilya Turchin. Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light. Remote Sensing 2021, 13, 1762 .
AMA StyleVladimir Sukhov, Ekaterina Sukhova, Andrey Khlopkov, Lyubov Yudina, Anastasiia Ryabkova, Alexander Telnykh, Ekaterina Sergeeva, Vladimir Vodeneev, Ilya Turchin. Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light. Remote Sensing. 2021; 13 (9):1762.
Chicago/Turabian StyleVladimir Sukhov; Ekaterina Sukhova; Andrey Khlopkov; Lyubov Yudina; Anastasiia Ryabkova; Alexander Telnykh; Ekaterina Sergeeva; Vladimir Vodeneev; Ilya Turchin. 2021. "Proximal Imaging of Changes in Photochemical Reflectance Index in Leaves Based on Using Pulses of Green-Yellow Light." Remote Sensing 13, no. 9: 1762.
Local damage (e.g., burning) induces a variation potential (VP), which is an important electrical signal in higher plants. A VP propagates into undamaged parts of the plant and influences numerous physiological processes, including photosynthesis. Rapidly increasing plant tolerance to stressors is likely to be a result of the physiological changes. Thus, developing methods of revealing VP-induced physiological changes can be used for the remote sensing of plant systemic responses to local damage. Previously, we showed that burning-induced VP influenced a photochemical reflectance index in pea leaves, but the influence of the electrical signals on other reflectance indices was not investigated. In this study, we performed a complex analysis of the influence of VP induction by local burning on difference reflectance indices based on 400–700 nm wavelengths in leaves of pea seedlings. Heat maps of the significance of local burning-induced changes in the reflectance indices and their correlations with photosynthetic parameters were constructed. Large spectral regions with significant changes in these indices after VP induction were revealed. Most changes were strongly correlated to photosynthetic parameters. Some indices, which can be potentially effective for revealing local burning-induced photosynthetic changes, are separately shown. Our results show that difference reflectance indices based on 400–700 nm wavelengths can potentially be used for the remote sensing of plant systemic responses induced by local damages and subsequent propagation of VPs.
Ekaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Vladimir Vodeneev; Vladimir Sukhov. Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings. Plants 2021, 10, 878 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Ekaterina Gromova, Anastasiia Ryabkova, Vladimir Vodeneev, Vladimir Sukhov. Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings. Plants. 2021; 10 (5):878.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Vladimir Vodeneev; Vladimir Sukhov. 2021. "Influence of Local Burning on Difference Reflectance Indices Based on 400–700 nm Wavelengths in Leaves of Pea Seedlings." Plants 10, no. 5: 878.
A drought, which can be often accompanied by increased temperature, is a key adverse factor for agricultural plants. Remote sensing of early plant changes under water shortage is a prospective way to improve plant cultivation; in particular, the sensing can be based on measurement of difference reflectance indices (RIs). We complexly analyzed the efficiency of RIs based on 400–700 nm wavelengths for revealing the influences of water shortage and short-term heating on plant seedlings. We measured spectra of reflected light in leaves of pea, wheat, and pumpkin under control and stress conditions. All possible RIs in the 400–700 nm range were calculated, significances of differences between experimental and control indices were estimated, and heatmaps of the significances were constructed. It was shown that the water shortage (pea seedlings) changed absolute values of large quantity of calculated RIs. Absolute values of some RIs were significantly changed for 1–5 or 2–5 days of the water shortage; they were strongly correlated to the potential quantum yield of photosystem II and relative water content in leaves. In contrast, the short-term heating (pea, wheat, and pumpkin seedlings) mainly influenced light-induced changes in RIs. Our results show new RIs, which are potentially sensitive to the action of stressors.
Ekaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Dmitry Kior; Vladimir Sukhov. Complex Analysis of the Efficiency of Difference Reflectance Indices on the Basis of 400–700 nm Wavelengths for Revealing the Influences of Water Shortage and Heating on Plant Seedlings. Remote Sensing 2021, 13, 962 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Ekaterina Gromova, Anastasiia Ryabkova, Dmitry Kior, Vladimir Sukhov. Complex Analysis of the Efficiency of Difference Reflectance Indices on the Basis of 400–700 nm Wavelengths for Revealing the Influences of Water Shortage and Heating on Plant Seedlings. Remote Sensing. 2021; 13 (5):962.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Anastasiia Ryabkova; Dmitry Kior; Vladimir Sukhov. 2021. "Complex Analysis of the Efficiency of Difference Reflectance Indices on the Basis of 400–700 nm Wavelengths for Revealing the Influences of Water Shortage and Heating on Plant Seedlings." Remote Sensing 13, no. 5: 962.
Electrical signals in plants caused by external stimuli are capable of inducing various physiological responses. The mechanisms of transformation of a long-distance electrical signal (ES) into a functional response remain largely unexplored and require additional research. In this work, we investigated the role of calcium ions in the development of ES-induced respiratory response. Gradual heating of the leaf causes the propagation of variation potential (VP) in the pea seedling. The propagation of VP leads to a transient activation of respiration in an unaffected leaf. During the VP generation, a transient increase in the intracellular calcium concentration takes place. A calcium channel blocker inhibits the respiratory response, and a calcium ionophore induces the activation of respiration. Inhibitory analysis has showed that the VP-induced increase in respiration activity is probably associated with calcium-mediated activation of rotenone-insensitive alternative NADPH dehydrogenases in mitochondria.
Andrey Khlopkov; Oksana Sherstneva; Maria Ladeynova; Marina Grinberg; Lyubov Yudina; Vladimir Sukhov; Vladimir Vodeneev. Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings. Plant Signaling & Behavior 2021, 16, 1869415 .
AMA StyleAndrey Khlopkov, Oksana Sherstneva, Maria Ladeynova, Marina Grinberg, Lyubov Yudina, Vladimir Sukhov, Vladimir Vodeneev. Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings. Plant Signaling & Behavior. 2021; 16 (4):1869415.
Chicago/Turabian StyleAndrey Khlopkov; Oksana Sherstneva; Maria Ladeynova; Marina Grinberg; Lyubov Yudina; Vladimir Sukhov; Vladimir Vodeneev. 2021. "Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings." Plant Signaling & Behavior 16, no. 4: 1869415.
Local damage (e.g., burning, heating, or crushing) causes the generation and propagation of a variation potential (VP), which is a unique electrical signal in higher plants. A VP influences numerous physiological processes, with photosynthesis and respiration being important targets. VP generation is based on transient inactivation of H+-ATPase in plasma membrane. In this work, we investigated the participation of this inactivation in the development of VP-induced photosynthetic and respiratory responses. Two- to three-week-old pea seedlings (Pisum sativum L.) and their protoplasts were investigated. Photosynthesis and respiration in intact seedlings were measured using a GFS-3000 gas analyzer, Dual-PAM-100 Pulse-Amplitude-Modulation (PAM)-fluorometer, and a Dual-PAM gas-exchange Cuvette 3010-Dual. Electrical activity was measured using extracellular electrodes. The parameters of photosynthetic light reactions in protoplasts were measured using the Dual-PAM-100; photosynthesis- and respiration-related changes in O2 exchange rate were measured using an Oxygraph Plus System. We found that preliminary changes in the activity of H+-ATPase in the plasma membrane (its inactivation by sodium orthovanadate or activation by fusicoccin) influenced the amplitudes and magnitudes of VP-induced photosynthetic and respiratory responses in intact seedlings. Decreases in H+-ATPase activity (sodium orthovanadate treatment) induced fast decreases in photosynthetic activity and increases in respiration in protoplasts. Thus, our results support the effect of H+-ATPase inactivation on VP-induced photosynthetic and respiratory responses.
Lyubov Yudina; Oksana Sherstneva; Ekaterina Sukhova; Marina Grinberg; Sergey Mysyagin; Vladimir Vodeneev; Vladimir Sukhov. Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas. Plants 2020, 9, 1585 .
AMA StyleLyubov Yudina, Oksana Sherstneva, Ekaterina Sukhova, Marina Grinberg, Sergey Mysyagin, Vladimir Vodeneev, Vladimir Sukhov. Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas. Plants. 2020; 9 (11):1585.
Chicago/Turabian StyleLyubov Yudina; Oksana Sherstneva; Ekaterina Sukhova; Marina Grinberg; Sergey Mysyagin; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Inactivation of H+-ATPase Participates in the Influence of Variation Potential on Photosynthesis and Respiration in Peas." Plants 9, no. 11: 1585.
Abscisic acid (ABA) is an important hormone in plants that participates in their acclimation to the action of stressors. Treatment by exogenous ABA and its synthetic analogs are a potential way of controlling the tolerance of agricultural plants; however, the mechanisms of influence of the ABA treatment on photosynthetic processes require further investigations. The aim of our work was to investigate the participation of inactivation of the plasma membrane H+-ATP-ase on the influence of ABA treatment on photosynthetic processes and their regulation by electrical signals in peas. The ABA treatment of seedlings was performed by spraying them with aqueous solutions (10−5 M). The combination of a Dual-PAM-100 PAM fluorometer and GFS-3000 infrared gas analyzer was used for photosynthetic measurements; the patch clamp system on the basis of a SliceScope Pro 2000 microscope was used for measurements of electrical activity. It was shown that the ABA treatment stimulated the cyclic electron flow around photosystem I and decreased the photosynthetic CO2 assimilation, the amplitude of burning-induced electrical signals (variation potentials), and the magnitude of photosynthetic responses relating to these signals; in contrast, treatment with exogenous ABA increased the heat tolerance of photosynthesis. An investigation of the influence of ABA treatment on the metabolic component of the resting potential showed that this treatment decreased the activity of the H+-ATP-ase in the plasma membrane. Inhibitor analysis using sodium orthovanadate demonstrated that this decrease may be a mechanism of the ABA treatment-induced changes in photosynthetic processes, their heat tolerance, and regulation by electrical signals.
Lyubov Yudina; Ekaterina Sukhova; Oksana Sherstneva; Marina Grinberg; Maria Ladeynova; Vladimir Vodeneev; Vladimir Sukhov. Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane. Biology 2020, 9, 324 .
AMA StyleLyubov Yudina, Ekaterina Sukhova, Oksana Sherstneva, Marina Grinberg, Maria Ladeynova, Vladimir Vodeneev, Vladimir Sukhov. Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane. Biology. 2020; 9 (10):324.
Chicago/Turabian StyleLyubov Yudina; Ekaterina Sukhova; Oksana Sherstneva; Marina Grinberg; Maria Ladeynova; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Exogenous Abscisic Acid Can Influence Photosynthetic Processes in Peas through a Decrease in Activity of H+-ATP-ase in the Plasma Membrane." Biology 9, no. 10: 324.
Electrical signals (ESs) can be induced by local action of stressors in plants; they influence numerous physiological processes (photosynthesis, transpiration, respiration, genes expression, synthesis of phytohormones, etc.) and, thereby, induce a systemic adaptation response. Development of optical methods of a remote sensing of this response can be important for agricultural and ecological monitoring. Preliminarily, we showed (Sukhova et al., Plant Sign Behav 2019; 14:e1610301) that burning-induced ESs induced changes in leaf reflectance at broad spectral bands (400-500, 500-600, 600-700, and 700-800 nm). The aims of the present work were (i) investigation of ESs influence on difference reflectance indices (RIs) calculated on the basis of these broad spectral bands and (ii) analysis of connection of the indices with water content in plants. Pea seedlings were investigated. ESs were induced by burning of the first mature leaf; ESs had high amplitudes in the second leaf and had low amplitudes in the fourth leaf. It was shown that ESs induced significant changes in RIs, which were calculated on basis of intensities of the reflected light at (i) 400-500 and 600-700 nm, (ii) 500-600 and 700-800 nm, and (iii) 600-700 and 700-800 nm. The effect was strong in the second leaf and weak in the fourth leaf; that is, the response was dependent on the magnitude of ESs. ESs-induced changes in RI were strongly connected with ESs-induced decrease of leaf water content which was estimated on basis of decrease of water index. Thus, broadband RIs can be used for revealing the ESs-induced systemic stress response in plants.
Ekaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves. Plant Signaling & Behavior 2020, 15, 1737786 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Ekaterina Gromova, Vladimir Nerush, Vladimir Vodeneev, Vladimir Sukhov. Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves. Plant Signaling & Behavior. 2020; 15 (4):1737786.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Ekaterina Gromova; Vladimir Nerush; Vladimir Vodeneev; Vladimir Sukhov. 2020. "Burning-induced electrical signals influence broadband reflectance indices and water index in pea leaves." Plant Signaling & Behavior 15, no. 4: 1737786.
Local action of stressors induces generation and propagation of electrical signals (ESs), which influence numerous physiological processes (including photosynthesis, expression of genes, production of phytohormones, etc.) in undamaged parts of plants; i.e. they induce a systemic stress response. Development of methods of remote sensing of this response (in particular, optical methods) is an important practical task for agricultural and ecological monitoring. However, this problem is not sufficiently researched. Earlier, we reported that ESs influence the photochemical reflectance index, which can be calculated on the basis of reflected light at 531 and 570 nm, and these changes are connected with photosynthetic changes. The aim of the current work is investigation of the influence of ESs on reflectance at broad spectral bands (400–500 nm, 500–600 nm, 600–700 nm and 700–800 nm). We showed that burning-induced ESs caused transient increase of intensity of reflected light at the all investigated spectral bands of visible light: reflectance at 600–700 nm had the maximal magnitude of changes and reflectance at 700–800 nm had the minimal magnitude of changes. Dynamics of the reflectance changes were distinguished from dynamics of photosynthetic changes, induced by ESs; i.e. ESs-induced changes in reflectance seem to be weakly connected with the photosynthetic response. Thus, our results show that changes in reflectance at broad spectral bands can also be used for remote sensing of the ESs-induced systemic stress response in plants.
Ekaterina Sukhova; Lyubov Yudina; Elena Akinchits; Vladimir Vodeneev; Vladimir Sukhov. Influence of electrical signals on pea leaf reflectance in the 400–800-nm range. Plant Signaling & Behavior 2019, 14, 1610301 .
AMA StyleEkaterina Sukhova, Lyubov Yudina, Elena Akinchits, Vladimir Vodeneev, Vladimir Sukhov. Influence of electrical signals on pea leaf reflectance in the 400–800-nm range. Plant Signaling & Behavior. 2019; 14 (7):1610301.
Chicago/Turabian StyleEkaterina Sukhova; Lyubov Yudina; Elena Akinchits; Vladimir Vodeneev; Vladimir Sukhov. 2019. "Influence of electrical signals on pea leaf reflectance in the 400–800-nm range." Plant Signaling & Behavior 14, no. 7: 1610301.
Local damages induce a wide spectrum of functional responses in intact parts of the plant, including changes in transpiration and CO2 assimilation, which may be associated with propagating variation potential (VP). In the present work carried out on pea (Pisum sativum L.) plants, an analysis of the influence of local burn leading to generation of VP on transpiration and CO2 assimilation under conditions of different air humidity was conducted. It was shown that VP induced multiphase changes in these physiological functions in intact pea leaf, including rapid lowering their parameters, slow elevation of them, and prolonged dropping transpiration and assimilation. Analysis of the impact of air humidity on these processes showed that quick and long lowering transpiration was significantly suppressed under the conditions of high humidity, while slowly increasing transpiration hardly depended on air humidity. Dependence of VP-induced CO2 assimilation response on air humidity exhibited a similar character. Additional correlation analysis of amplitudes of VP-induced transpiration and CO2 assimilation changes showed that prolonged stomata closing may be the mechanism of long-term lowering assimilation of intact leaves after the action of local burn. In general, it was established that the VP-induced transpiration response is a complex combination of activation and suppression of transpiration, and the contribution of different components to the observed responses depends on air humidity.
L. M. Yudina; O. N. Sherstneva; S. A. Mysyagin; Vladimir Vodeneev; V. S. Sukhov. Impact of Local Damage on Transpiration of Pea Leaves at Various Air Humidity. Russian Journal of Plant Physiology 2019, 66, 87 -94.
AMA StyleL. M. Yudina, O. N. Sherstneva, S. A. Mysyagin, Vladimir Vodeneev, V. S. Sukhov. Impact of Local Damage on Transpiration of Pea Leaves at Various Air Humidity. Russian Journal of Plant Physiology. 2019; 66 (1):87-94.
Chicago/Turabian StyleL. M. Yudina; O. N. Sherstneva; S. A. Mysyagin; Vladimir Vodeneev; V. S. Sukhov. 2019. "Impact of Local Damage on Transpiration of Pea Leaves at Various Air Humidity." Russian Journal of Plant Physiology 66, no. 1: 87-94.