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The objective of this study was the development and design of a treatment system at a pilot-plant scale for the remediation of hydrocarbons in industrial wastewater. The treatment consists of a combined approach of absorption and biodegradation to obtain treated water with sufficient quality to be reused in fire defense systems (FDSs). The plant consists of four vertical flow columns (bioreactors) made of stainless steel (ATEX Standard) with dimensions of 1.65 × 0.5 m and water volumes of 192.4 L. Each bioreactor includes a holder to contain the absorbent material (Pad Sentec polypropylene). The effectiveness of the treatment system has been studied in wastewater with high and low pollutant loads (concentrations higher than 60,000 mg L−1 of total petroleum hydrocarbons (TPH) and lower than 500 mg L−1 of TPHs, respectively). The pilot-plant design can function at two different flow rates, Q1 (180 L h−1) and Q2 (780 L h−1), with or without additional aeration. The results obtained for strongly polluted wastewaters showed that, at low flow rates, additional aeration enhanced hydrocarbon removal, while aeration was unnecessary at high flow rates. For wastewater with a low pollutant load, we selected a flow rate of 780 L h−1 without aeration. Different recirculation times were also tested along with the application of a post-treatment lasting 7 days inside the bioreactor without recirculation. The microbial diversity studies showed similar populations of bacteria and fungi in the inlet and outlet wastewater. Likewise, high similarity indices were observed between the adhered and suspended biomass within the bioreactors. The results showed that the setup and optimization of the reactor represent a step forward in the application of bioremediation processes at an industrial/large scale.
Gloria Silva-Castro; Alfonso Rodríguez-Calvo; Tatiana Robledo-Mahón; Elisabet Aranda; Jesús González-López; Concepción Calvo. Design of Bio-Absorbent Systems for the Removal of Hydrocarbons from Industrial Wastewater: Pilot-Plant Scale. Toxics 2021, 9, 162 .
AMA StyleGloria Silva-Castro, Alfonso Rodríguez-Calvo, Tatiana Robledo-Mahón, Elisabet Aranda, Jesús González-López, Concepción Calvo. Design of Bio-Absorbent Systems for the Removal of Hydrocarbons from Industrial Wastewater: Pilot-Plant Scale. Toxics. 2021; 9 (7):162.
Chicago/Turabian StyleGloria Silva-Castro; Alfonso Rodríguez-Calvo; Tatiana Robledo-Mahón; Elisabet Aranda; Jesús González-López; Concepción Calvo. 2021. "Design of Bio-Absorbent Systems for the Removal of Hydrocarbons from Industrial Wastewater: Pilot-Plant Scale." Toxics 9, no. 7: 162.
Aerobic granular sludge (AGS) comprises an aggregation of microbial cells in a tridimensional matrix, which is able to remove carbon, nitrogen and phosphorous as well as other pollutants in a single bioreactor under the same operational conditions. During the past decades, the feasibility of implementing AGS in wastewater treatment plants (WWTPs) for treating sewage using fundamentally sequential batch reactors (SBRs) has been studied. However, granular sludge technology using SBRs has several disadvantages. For instance, it can present certain drawbacks for the treatment of high flow rates; furthermore, the quantity of retained biomass is limited by volume exchange. Therefore, the development of continuous flow reactors (CFRs) has come to be regarded as a more competitive option. This is why numerous investigations have been undertaken in recent years in search of different designs of CFR systems that would enable the effective treatment of urban and industrial wastewater, keeping the stability of granular biomass. However, despite these efforts, satisfactory results have yet to be achieved. Consequently, it remains necessary to carry out new technical approaches that would provide more effective and efficient AGS-CFR systems. In particular, it is imperative to develop continuous flow granular systems that can both retain granular biomass and efficiently treat wastewater, obviously with low construction, maintenance and exploitation cost. In this review, we collect the most recent information on different technological approaches aimed at establishing AGS-CFR systems, making possible their upscaling to real plant conditions. We discuss the advantages and disadvantages of these proposals and suggest future trends in the application of aerobic granular systems. Accordingly, we analyze the most significant technical and biological implications of this innovative technology.
Aurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water 2021, 13, 1792 .
AMA StyleAurora Rosa-Masegosa, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Massimiliano Fenice, Susanna Gorrasi, Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water. 2021; 13 (13):1792.
Chicago/Turabian StyleAurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. 2021. "New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects." Water 13, no. 13: 1792.
Enterobacteriaceae is present in various niches worldwide (i.e., the gastrointestinal tracts of animals, clinical specimens, and diverse environments) and hosts some well-known pathogens (i.e., salmonellas, shigellas and pathogenic coliforms). No investigation has focused on its occurrence in marine salterns, and it is not clear if these hypersaline environments could be a reservoir for these bacteria including some potentially harmful members. In this study, a two-year metabarcoding survey was carried out on samples collected from different ponds of the “Saline di Tarquinia” salterns and the nearby coastal waters. Enterobacteriaceae was recorded almost constantly in the seawaters feeding the saltern. Its abundance was generally higher in the sea than in the ponds, probably due to the higher anthropic impact. The same trend was evidenced for the key genus (Escherichia/Shigella) and OTU (OTU 5) of the Enterobacteriaceae community. Various parameters affected taxon/OTU abundance: Enterobacteriaceae, Escherichia/Shigella and OTU5 decreased with increasing salinity and rains; moreover, Escherichia/Shigella and OTU 5 were higher in autumn than in spring. Although Enterobacteriaceae did not seem to find the most favourable conditions for a high-abundance persistence in the saltern environment, it did not disappear. These observations suggested this environment as a potential reservoir for bacteria with possible important health implications.
Susanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water 2021, 13, 1443 .
AMA StyleSusanna Gorrasi, Marcella Pasqualetti, Andrea Franzetti, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez, Barbara Muñoz-Palazon, Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water. 2021; 13 (11):1443.
Chicago/Turabian StyleSusanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. 2021. "Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone." Water 13, no. 11: 1443.
The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during wastewater treatment leads to concerns about whether this process may represent a focal point for the transmission of COVID-19. An epidemiological analysis, based on a COVID-19 IgG/IgM Rapid Test Cassette, performed on 134 wastewater workers from 59 wastewater treatment plants from the province of Granada (Spain) showed a seroprevalence of 8.95% in IgG for SARS-CoV-2, which is similar to the incidence rate found for the general population of the province (9.6%; 95%CI = 7.2–12.8). These findings suggests that current safety measures are sufficient for the protection of workers against SARS-CoV-2.
B. Muñoz-Palazon; P. R. Bouzas; J. González-López; M. Manzanera. Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study. International Journal of Water Resources Development 2021, 1 -10.
AMA StyleB. Muñoz-Palazon, P. R. Bouzas, J. González-López, M. Manzanera. Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study. International Journal of Water Resources Development. 2021; ():1-10.
Chicago/Turabian StyleB. Muñoz-Palazon; P. R. Bouzas; J. González-López; M. Manzanera. 2021. "Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study." International Journal of Water Resources Development , no. : 1-10.
Two aerobic granular sludge (AGS) sequential batch reactors were operated at a mild (15 °C) temperature for 180 days. One of those bioreactors was exposed to a mixture of diclofenac, naproxen, trimethoprim, and carbamazepine. The AGS system, operating under pressure from emerging contaminants, showed a decrease in COD, BOD5, and TN removal capacity, mainly observed during the first 100 days, in comparison with the removal ratios detected in the control bioreactor. After an acclimatisation period, the removal reached high-quality effluent for COD and TN, close to 95% and 90%, respectively. In the steady-state period, trimethoprim and diclofenac were successfully removed with values around 50%, while carbamazepine and naproxen were more recalcitrant. The dominant bacterial OTUs were affected by the presence of a mixture of pharmaceutical compounds, under which the dominant phylotypes changed to OTUs classified among the Pseudomonas, Gemmobacter, and Comamonadaceae. The RT-qPCR and qPCR results showed the deep effects of pharmaceutical compounds on the number of copies of target genes. Statistical analyses allowed for linking the total and active microbial communities with the physico-chemical performance, describing the effects of pharmaceutical compounds in pollution degradation, as well as the successful adaptation of the system to treat wastewater in the presence of toxic compounds.
Barbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics 2021, 9, 93 .
AMA StyleBarbara Muñoz-Palazon, Aurora Rosa-Masegosa, Miguel Hurtado-Martinez, Alejandro Rodriguez-Sanchez, Alexander Link, Ramiro Vilchez-Vargas, Alejandro Gonzalez-Martinez, Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics. 2021; 9 (5):93.
Chicago/Turabian StyleBarbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. 2021. "Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds." Toxics 9, no. 5: 93.
Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denitrificans Z1, and P. fluorescens PSC26, previously reported as denitrifying microorganisms carrying the nosZ gene. Pseudomonas denitrificans Z1 produced fluffy, low-density granules, with a decantation speed below 10 m h−1. However, P. fluorescens PSC26, P. stutzeri I1, and P. fluorescens 376 formed stable granules, with mean size from 7 to 15 mm, related to the strain and carbon concentration. P. stutzeri I1 and P. fluorescens 376 removed nitrate efficiently with a ratio in the range of 96%, depending on the source and concentration of organic matter. Therefore, the findings suggest that the inoculation of GSBR systems with denitrifying strains of Pseudomonas spp. containing the nosZ gene enables the formation of stable granules, the efficient removal of nitrate, and the transformation of nitrate into nitrogen gas, a result of considerable environmental interest to avoid the generation of nitrous oxide.
Miguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water 2021, 13, 1119 .
AMA StyleMiguel Hurtado-Martinez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maximino Manzanera, Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water. 2021; 13 (8):1119.
Chicago/Turabian StyleMiguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. 2021. "Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors." Water 13, no. 8: 1119.
In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an important role in the microbial dynamics in wastewater treatment systems and the biosafety of effluents. In this work, the viral members present in influent wastewater, mixed liquor (aerobic bioreactor), excess sludge, and effluent water of a conventional activated sludge system for the treatment of urban wastewater were identified. Viral members were observed by transmission electron microscopy and studied through next-generation sequencing studies. The results showed the dominance of bacteriophages in the viral community in all samples, with the dominant viral phylotype classified as Escherichia coli O157 typing phage 7. Moreover, different human viruses, such as Cynomolgus cytomegalovirus and Gammaherpesvirus, were also detected.
Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water 2020, 12, 2316 .
AMA StyleCristina García-Fontana, Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maria Vela-Cano, Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water. 2020; 12 (8):2316.
Chicago/Turabian StyleCristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. 2020. "Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain." Water 12, no. 8: 2316.
Non-steroidal anti-inflammatory drugs (NSAIDs) and analgesics are two of the most employed drug groups around the world due to their use in the treatment of edema and pain. However, they also present an ecological challenge because they are considered as potential water pollutants. In this work, the biodegradation of four NSAIDs (diclofenac, ibuprofen, naproxen and ketoprofen) and one analgesic (acetaminophen) at 50 µM (initial concentration) by Penicillium oxalicum, at both flask and bioreactor bench scales, was evaluated. An important co-metabolic mechanism as part of the global bioremediation process for the elimination of these drugs was observed, as in some cases it was necessary to supplement glucose to achieve a 100% removal rate: both individually and as a complex mixture. Identical behavior in the implementation of a fluidized bench-scale batch bioreactor, inoculated with pellets of this fungus and the complex mix of the drugs, was observed. The role of the cytochrome P450 enzymes (CYP) in the biodegradation of the drugs mix were evidenced by the observation of hydroxylated by-products. The results on the reduction of toxicity (micro and phyto) were not conclusive; however, a reduction in phytotoxicity was detected.
Darío Rafael Olicón-Hernández; Maite Ortúzar; Clementina Pozo; Jesús González-López; Elisabet Aranda. Metabolic Capability of Penicillium oxalicum to Transform High Concentrations of Anti-Inflammatory and Analgesic Drugs. Applied Sciences 2020, 10, 2479 .
AMA StyleDarío Rafael Olicón-Hernández, Maite Ortúzar, Clementina Pozo, Jesús González-López, Elisabet Aranda. Metabolic Capability of Penicillium oxalicum to Transform High Concentrations of Anti-Inflammatory and Analgesic Drugs. Applied Sciences. 2020; 10 (7):2479.
Chicago/Turabian StyleDarío Rafael Olicón-Hernández; Maite Ortúzar; Clementina Pozo; Jesús González-López; Elisabet Aranda. 2020. "Metabolic Capability of Penicillium oxalicum to Transform High Concentrations of Anti-Inflammatory and Analgesic Drugs." Applied Sciences 10, no. 7: 2479.
Wastewaters polluted with hydrocarbons are an environmental problem that has a significant impact on the natural ecosystem and on human health. Thus, the aim of this research was to develop a bioreactor sorbent technology for treating these polluted waters. A lab-scale plant composed of three 1-L bioreactors with different sorbent materials inside (meltblown polypropylene and granulated cork) was built. Wastewater to be treated was recirculated through each bioreactor for 7 days. Results showed that hydrocarbon retention rates in the three bioreactors ranged between 92.6% and 94.5% of total petroleum hydrocarbons (TPHs) and that after one simple recirculation cycle, no hydrocarbon fractions were detected by gas chromatography/Mass Spectrometry (GC/MS) in the effluent wastewater. In addition, after the wastewater treatment, the sorbent materials were extracted from the bioreactors and deposited in vessels to study the biodegradation of the retained hydrocarbons by the wastewater indigenous microbiota adhered to sorbents during the wastewater treatment. A TPH removal of 41.2% was detected after one month of Pad Sentec™ carrier treatment. Further, the shifts detected in the percentages of some hydrocarbon fractions suggested that biodegradation is at least partially involved in the hydrocarbon removal process. These results proved the efficiency of this technology for the treatment of these hydrocarbon-polluted-waters.
Alfonso Rodríguez Calvo; Gloria Andrea Silva-Castro; Darío Rafael Olicón-Hernández; Jesús González-López; Concepción Calvo. Biodegradation and Absorption Technology for Hydrocarbon-Polluted Water Treatment. Applied Sciences 2020, 10, 841 .
AMA StyleAlfonso Rodríguez Calvo, Gloria Andrea Silva-Castro, Darío Rafael Olicón-Hernández, Jesús González-López, Concepción Calvo. Biodegradation and Absorption Technology for Hydrocarbon-Polluted Water Treatment. Applied Sciences. 2020; 10 (3):841.
Chicago/Turabian StyleAlfonso Rodríguez Calvo; Gloria Andrea Silva-Castro; Darío Rafael Olicón-Hernández; Jesús González-López; Concepción Calvo. 2020. "Biodegradation and Absorption Technology for Hydrocarbon-Polluted Water Treatment." Applied Sciences 10, no. 3: 841.
Two pilot-scale hybrid moving bed biofilm reactor-membrane bioreactors were operated in parallel for the treatment of salinity-amended urban wastewater under 6 hours of hydraulic retention time and 2500 mg L−1 total solids concentration. Two salinity conditions were tested: the constant salinity of 6.5 mS cm−1 electric conductivity (3.6 g L−1 NaCl) and the tidal-like variable salinity with maximum 6.5 mS cm−1 electric conductivity. An investigation was developed on the biofouling produced on the ultrafiltration membrane surface evaluating its bacterial community structure and its potential function in the fouling processes. The results showed that biofouling was clearly affected by salinity scenarios in terms of α-diversity and β-diversity and bacterial community structure, which confirms lower bacterial diversity under variable salinity conditions with Rhodanobacter and Dyella as dominant phylotypes. Microorganisms identified as bio-mineral formers belonged to genera Bacillus, Citrobacter, and Brevibacterium. These findings will be of help for the prevention and control of biofouling in saline wastewater treatment systems.
Alejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water 2018, 10, 1133 .
AMA StyleAlejandro Rodriguez-Sanchez, Juan Carlos Leyva-Diaz, Barbara Muñoz-Palazon, Maria Angustias Rivadeneyra, Miguel Hurtado-Martinez, Daniel Martin-Ramos, Alejandro Gonzalez-Martinez, Jose Manuel Poyatos, Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water. 2018; 10 (9):1133.
Chicago/Turabian StyleAlejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. 2018. "Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration." Water 10, no. 9: 1133.
Waste treatment and the simultaneous production of energy have gained great interest in the world. In the last decades, scientific efforts have focused largely on improving and developing sustainable bioprocess solutions for energy recovery from challenging waste. Anaerobic digestion (AD) has been developed as a low-cost organic waste treatment technology with a simple setup and relatively limited investment and operating costs. Different technologies such as one-stage and two-stage AD have been developed. The viability and performance of these technologies have been extensively reported, showing the supremacy of two-stage AD in terms of overall energy recovery from biomass under different substrates, temperatures, and pH conditions. However, a comprehensive review of the advantages and disadvantages of these technologies is still lacking. Since microbial ecology is critical to developing successful AD, many studies have shown the structure and dynamics of archaeal and bacterial communities in this type of system. However, the role of Eukarya groups remains largely unknown to date. In this review, we provide a comprehensive review of the role, abundance, dynamics, and structure of archaeal, bacterial, and eukaryal communities during the AD process. The information provided could help researchers to select the adequate operational parameters to obtain the best performance and biogas production results.
Antonio Castellano-Hinojosa; Caterina Armato; Clementina Pozo; Alejandro Gonzalez-Martinez; Jesús González-López. New concepts in anaerobic digestion processes: recent advances and biological aspects. Applied Microbiology and Biotechnology 2018, 102, 5065 -5076.
AMA StyleAntonio Castellano-Hinojosa, Caterina Armato, Clementina Pozo, Alejandro Gonzalez-Martinez, Jesús González-López. New concepts in anaerobic digestion processes: recent advances and biological aspects. Applied Microbiology and Biotechnology. 2018; 102 (12):5065-5076.
Chicago/Turabian StyleAntonio Castellano-Hinojosa; Caterina Armato; Clementina Pozo; Alejandro Gonzalez-Martinez; Jesús González-López. 2018. "New concepts in anaerobic digestion processes: recent advances and biological aspects." Applied Microbiology and Biotechnology 102, no. 12: 5065-5076.
A membrane bioreactor (MBR) and a hybrid moving bed biofilm reactor-membrane bioreactor (hybrid MBBR-MBR) for municipal wastewater treatment were studied to determine the effect of salinity on nitrogen removal and autotrophic kinetics. The biological systems were analyzed during the start-up phase with a hydraulic retention time (HRT) of 6 h, total biomass concentration of 2,500 mg L−1 in the steady state, and electric conductivities of 1.05 mS cm−1 for MBR and hybrid MBBR-MBR working under regular salinity and conductivity variations of 1.2–6.5 mS cm−1 for MBR and hybrid MBBR-MBR operating at variable salinity. The variable salinity affected the autotrophic biomass, which caused a reduction of the nitrogen degradation rate, an increase of time to remove ammonium from municipal wastewater and longer duration of the start-up phase for the MBR and hybrid MBBR-MBR.
J. C. Leyva-Díaz; Alejandro Rodriguez-Sanchez; J. González-López; J. M. Poyatos. Effect of salinity variation on the autotrophic kinetics of the start-up of a membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor at low hydraulic retention time. Water Science and Technology 2017, 77, 714 -720.
AMA StyleJ. C. Leyva-Díaz, Alejandro Rodriguez-Sanchez, J. González-López, J. M. Poyatos. Effect of salinity variation on the autotrophic kinetics of the start-up of a membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor at low hydraulic retention time. Water Science and Technology. 2017; 77 (3):714-720.
Chicago/Turabian StyleJ. C. Leyva-Díaz; Alejandro Rodriguez-Sanchez; J. González-López; J. M. Poyatos. 2017. "Effect of salinity variation on the autotrophic kinetics of the start-up of a membrane bioreactor and hybrid moving bed biofilm reactor-membrane bioreactor at low hydraulic retention time." Water Science and Technology 77, no. 3: 714-720.
Community structure, population dynamics and diversity of fungi were monitored in a full-scale membrane bioreactor (MBR) operated throughout four experimental phases (Summer 2009, Autumn 2009, Summer 2010 and Winter, 2012) under different conditions, using the 18S-rRNA gene and the intergenic transcribed spacer (ITS2-region) as molecular markers, and a combination of temperature-gradient gel electrophoresis and 454-pyrosequencing. Both total and metabolically-active fungal populations were fingerprinted, by amplification of molecular markers from community DNA and retrotranscribed RNA, respectively. Fingerprinting and 454-pyrosequencing evidenced that the MBR sheltered a dynamic fungal community composed of a low number of species, in accordance with the knowledge of fungal diversity in freshwater environments, and displaying a medium-high level of functional organization with few numerically dominant phylotypes. Population shifts were experienced in strong correlation with the changes of environmental variables and operation parameters, with pH contributing the highest level of explanation. Phylotypes assigned to nine different fungal Phyla were detected, although the community was mainly composed of Ascomycota, Basidiomycota and Chytridiomycota/Blastocladiomycota. Prevailing fungal phylotypes were affiliated to Saccharomycetes and Chytridiomycetes/Blastocladiomycetes, which displayed antagonistic trends in their relative abundance throughout the experimental period. Fungi identified in the activated sludge were closely related to genera of relevance for the degradation of organic matter and trace-organic contaminants, as well as genera of dimorphic fungi potentially able to produce plant operational issues such as foaming or biofouling. Phylotypes closely related to genera of human and plant pathogenic fungi were also detected.
P. Maza-Márquez; R. Vilchez-Vargas; Frederiek-Maarten Kerckhof; Elisabet Aranda; J. González-López; Belén Rodelas. Community structure, population dynamics and diversity of fungi in a full-scale membrane bioreactor (MBR) for urban wastewater treatment. Water Research 2016, 105, 507 -519.
AMA StyleP. Maza-Márquez, R. Vilchez-Vargas, Frederiek-Maarten Kerckhof, Elisabet Aranda, J. González-López, Belén Rodelas. Community structure, population dynamics and diversity of fungi in a full-scale membrane bioreactor (MBR) for urban wastewater treatment. Water Research. 2016; 105 ():507-519.
Chicago/Turabian StyleP. Maza-Márquez; R. Vilchez-Vargas; Frederiek-Maarten Kerckhof; Elisabet Aranda; J. González-López; Belén Rodelas. 2016. "Community structure, population dynamics and diversity of fungi in a full-scale membrane bioreactor (MBR) for urban wastewater treatment." Water Research 105, no. : 507-519.
The abundance of total and metabolically active populations of Mycolata was evaluated in a full-scale membrane bioreactor (MBR) experiencing seasonal foaming, using quantitative PCR (qPCR) and retrotranscribed qPCR (RT-qPCR) targeting the 16S rRNA gene sequence. While the abundance of total Mycolata remained stable (1010 copies of 16S rRNA genes/L activated sludge) throughout four different experimental phases, significant variations (up to one order of magnitude) were observed when the 16S rRNA was targeted. The highest ratios of metabolically active versus total Mycolata populations were observed in samples of two experimental phases when foaming was experienced in the MBR. Non-metric multidimensional scaling and BIO-ENV analyses demonstrated that this ratio was positively correlated to the concentrations of substrates in the influent water, F/M ratio, and pH, and negatively correlated to temperature and solids retention time. It the first time that the ratio of metabolically active versus total Mycolata is found to be a key parameter triggering foaming in the MBR; thus, we propose it as a candidate predictive tool.
P. Maza-Márquez; R. Vílchez-Vargas; Nico Boon; J. González-López; M.V. Martínez-Toledo; Belén Rodelas. The ratio of metabolically active versus total Mycolata populations triggers foaming in a membrane bioreactor. Water Research 2016, 92, 208 -217.
AMA StyleP. Maza-Márquez, R. Vílchez-Vargas, Nico Boon, J. González-López, M.V. Martínez-Toledo, Belén Rodelas. The ratio of metabolically active versus total Mycolata populations triggers foaming in a membrane bioreactor. Water Research. 2016; 92 ():208-217.
Chicago/Turabian StyleP. Maza-Márquez; R. Vílchez-Vargas; Nico Boon; J. González-López; M.V. Martínez-Toledo; Belén Rodelas. 2016. "The ratio of metabolically active versus total Mycolata populations triggers foaming in a membrane bioreactor." Water Research 92, no. : 208-217.
High concentrations of proteins and amino acids can be found in wastewater and wastewater stream produced in anaerobic digesters, having shown that amino acids could persist over different managements for nitrogen removal affecting the nitrogen removal processes. Nitrogen removal is completely necessary because of their implications and the significant adverse environmental impact of ammonium such as eutrophication and toxicity to aquatic life on the receiving bodies. In the last decade, the treatment of effluents with high ammonium concentration through anammox-based bioprocesses has been enhanced because these biotechnologies are cheaper and more environmentally friendly than conventional technologies. However, it has been shown that the presence of important amounts of proteins and amino acids in the effluents seriously affects the microbial autotrophic consortia leading to important losses in terms of ammonium oxidation efficiency. Particularly the presence of sulfur amino acids such as methionine and cysteine has been reported to drastically decrease the autotrophic denitrification processes as well as affect the microbial community structure promoting the decline of ammonium oxidizing bacteria in favor of other phylotypes. In this context we discuss that new biotechnological processes that improve the degradation of protein and amino acids must be considered as a priority to increase the performance of the autotrophic denitrification biotechnologies.
Alejandro Gonzalez-Martinez; Kadiya Calderon; Jesús González-López. New concepts of microbial treatment processes for the nitrogen removal: effect of protein and amino acids degradation. Amino Acids 2016, 48, 1123 -1130.
AMA StyleAlejandro Gonzalez-Martinez, Kadiya Calderon, Jesús González-López. New concepts of microbial treatment processes for the nitrogen removal: effect of protein and amino acids degradation. Amino Acids. 2016; 48 (5):1123-1130.
Chicago/Turabian StyleAlejandro Gonzalez-Martinez; Kadiya Calderon; Jesús González-López. 2016. "New concepts of microbial treatment processes for the nitrogen removal: effect of protein and amino acids degradation." Amino Acids 48, no. 5: 1123-1130.
C. Cortés-Lorenzo; Alejandro Gonzalez-Martinez; Hauke Smidt; J. González-López; Belén Rodelas. Influence of salinity on fungal communities in a submerged fixed bed bioreactor for wastewater treatment. Chemical Engineering Journal 2016, 285, 562 -572.
AMA StyleC. Cortés-Lorenzo, Alejandro Gonzalez-Martinez, Hauke Smidt, J. González-López, Belén Rodelas. Influence of salinity on fungal communities in a submerged fixed bed bioreactor for wastewater treatment. Chemical Engineering Journal. 2016; 285 ():562-572.
Chicago/Turabian StyleC. Cortés-Lorenzo; Alejandro Gonzalez-Martinez; Hauke Smidt; J. González-López; Belén Rodelas. 2016. "Influence of salinity on fungal communities in a submerged fixed bed bioreactor for wastewater treatment." Chemical Engineering Journal 285, no. : 562-572.
The community structure and population dynamics of Mycolata were monitored in a full-scale membrane bioreactor during four experimental phases under changing operating and environmental conditions, by means of temperature-gradient gel electrophoresis of partial 16S-rRNA genes amplified from community DNA and RNA templates (total and active populations). Non-metric multidimensional scaling and BIO-ENV analyses demonstrated that population dynamics were mostly explained (30-32%) by changes in the input of nutrients in the influent water and the accumulation of biomass in the bioreactors, while the influence of hydraulic and solid retention times, temperature and F/M ratio was minor. Significant correlations were observed between particular Mycolata phylotypes and one or more variables, contributing information for the prediction of their abundance and activity under changing conditions. Fingerprinting and multivariate analyses demonstrated that two foaming episodes, recorded at temperatures <20°C, were connected to the increase of the relative abundance of Mycolata unrelated to Gordonia amarae.
P. Maza-Márquez; Cinta Gomez-Silvan; M.A. Gómez; J. González-López; M.V. Martínez-Toledo; Belén Rodelas. Linking operation parameters and environmental variables to population dynamics of Mycolata in a membrane bioreactor. Bioresource Technology 2015, 180, 318 -329.
AMA StyleP. Maza-Márquez, Cinta Gomez-Silvan, M.A. Gómez, J. González-López, M.V. Martínez-Toledo, Belén Rodelas. Linking operation parameters and environmental variables to population dynamics of Mycolata in a membrane bioreactor. Bioresource Technology. 2015; 180 ():318-329.
Chicago/Turabian StyleP. Maza-Márquez; Cinta Gomez-Silvan; M.A. Gómez; J. González-López; M.V. Martínez-Toledo; Belén Rodelas. 2015. "Linking operation parameters and environmental variables to population dynamics of Mycolata in a membrane bioreactor." Bioresource Technology 180, no. : 318-329.
I.M. Guisado; Jessica Purswani; J. Gonzalez-Lopez; Clementina Pozo. Physiological and genetic screening methods for the isolation of methyl tert-butyl ether-degrading bacteria for bioremediation purposes. International Biodeterioration & Biodegradation 2015, 97, 67 -74.
AMA StyleI.M. Guisado, Jessica Purswani, J. Gonzalez-Lopez, Clementina Pozo. Physiological and genetic screening methods for the isolation of methyl tert-butyl ether-degrading bacteria for bioremediation purposes. International Biodeterioration & Biodegradation. 2015; 97 ():67-74.
Chicago/Turabian StyleI.M. Guisado; Jessica Purswani; J. Gonzalez-Lopez; Clementina Pozo. 2015. "Physiological and genetic screening methods for the isolation of methyl tert-butyl ether-degrading bacteria for bioremediation purposes." International Biodeterioration & Biodegradation 97, no. : 67-74.
Nitrogen is a main contaminant of wastewater worldwide. Novel processes for nitrogen removal have been developed over the last several decades. One of these is the partial nitritation process. This process includes the oxidation of ammonium to nitrite without the generation of nitrate. The partial nitritation process has several advantages over traditional nitrification-denitrification processes for nitrogen removal from wastewaters. In addition, partial nitritation is required for anammox elimination of nitrogen from wastewater. Partial nitritation is affected by operational conditions and substances present in the influent, such as quinolone antibiotics. In this review, the impact that several operational conditions, such as temperature, pH, dissolved oxygen concentration, hydraulic retention time and solids retention time, have over the partial nitritation process is covered. The effect of quinolone antibiotics and other emerging contaminants are discussed. Finally, future perspectives for the partial nitritation process are commented upon.
Alejandro Rodriguez-Sanchez; Alejandro Gonzalez-Martinez; Maria Victoria Martinez-Toledo; Maria Jesus Garcia-Ruiz; Francisco Osorio; Jesús González-López. The Effect of Influent Characteristics and Operational Conditions over the Performance and Microbial Community Structure of Partial Nitritation Reactors. Water 2014, 6, 1905 -1924.
AMA StyleAlejandro Rodriguez-Sanchez, Alejandro Gonzalez-Martinez, Maria Victoria Martinez-Toledo, Maria Jesus Garcia-Ruiz, Francisco Osorio, Jesús González-López. The Effect of Influent Characteristics and Operational Conditions over the Performance and Microbial Community Structure of Partial Nitritation Reactors. Water. 2014; 6 (7):1905-1924.
Chicago/Turabian StyleAlejandro Rodriguez-Sanchez; Alejandro Gonzalez-Martinez; Maria Victoria Martinez-Toledo; Maria Jesus Garcia-Ruiz; Francisco Osorio; Jesús González-López. 2014. "The Effect of Influent Characteristics and Operational Conditions over the Performance and Microbial Community Structure of Partial Nitritation Reactors." Water 6, no. 7: 1905-1924.
The ability of bacteria isolated from a fixed-film bioreactor to precipitate phosphate crystals for the treatment of domestic wastewater in both artificial and natural media was studied. When this was demonstrated in artificial solid media for crystal formation, precipitation took place rapidly, and crystal formation began 3 days after inoculation. The percentage of phosphate-forming bacteria was slightly higher than 75%. Twelve major colonies with phosphate precipitation capacity were the dominant heterotrophic platable bacteria growing aerobically in artificial media. According to their taxonomic affiliations (based on partial sequencing of the 16S rRNA), the 12 strains belonged to the following genera of Gram-negative bacteria: Rhodobacter, Pseudoxanthobacter, Escherichia, Alcaligenes, Roseobacter, Ochrobactrum, Agromyce, Sphingomonas and Paracoccus. The phylogenetic tree shows that most of the identified populations were evolutionarily related to the Alphaproteobacteria (91.66% of sequences). The minerals formed were studied by X-ray diffraction, scanning electron microscopy (SEM), and energy dispersive X-ray microanalysis (EDX). All of these strains formed phosphate crystals and precipitated struvite (MgNH4PO4·6H2O), bobierrite [Mg3(PO4)2·8H2O] and baricite [(MgFe)3(PO4)2·8H2O]. The results obtained in this study show that struvite and spherulite crystals did not show any cell marks. Moreover, phosphate precipitation was observed in the bacterial mass but also near the colonies. Our results suggest that the microbial population contributed to phosphate precipitation by changing the media as a consequence of their metabolic activity. Moreover, the results of this research suggest that bacteria play an active role in the mineral precipitation of soluble phosphate from urban wastewater in submerged fixed-film bioreactors.
Almudena Rivadeneyra; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Daniel Martin-Ramos; Maria Victoria Martinez-Toledo; Maria Angustias Rivadeneyra. Precipitation of Phosphate Minerals by Microorganisms Isolated from a Fixed-Biofilm Reactor Used for the Treatment of Domestic Wastewater. International Journal of Environmental Research and Public Health 2014, 11, 3689 -3704.
AMA StyleAlmudena Rivadeneyra, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez, Daniel Martin-Ramos, Maria Victoria Martinez-Toledo, Maria Angustias Rivadeneyra. Precipitation of Phosphate Minerals by Microorganisms Isolated from a Fixed-Biofilm Reactor Used for the Treatment of Domestic Wastewater. International Journal of Environmental Research and Public Health. 2014; 11 (4):3689-3704.
Chicago/Turabian StyleAlmudena Rivadeneyra; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Daniel Martin-Ramos; Maria Victoria Martinez-Toledo; Maria Angustias Rivadeneyra. 2014. "Precipitation of Phosphate Minerals by Microorganisms Isolated from a Fixed-Biofilm Reactor Used for the Treatment of Domestic Wastewater." International Journal of Environmental Research and Public Health 11, no. 4: 3689-3704.