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Aerobic granular sludge (AGS) comprises an aggregation of microbial cells in a tridimensional matrix, which is able to remove carbon, nitrogen and phosphorous as well as other pollutants in a single bioreactor under the same operational conditions. During the past decades, the feasibility of implementing AGS in wastewater treatment plants (WWTPs) for treating sewage using fundamentally sequential batch reactors (SBRs) has been studied. However, granular sludge technology using SBRs has several disadvantages. For instance, it can present certain drawbacks for the treatment of high flow rates; furthermore, the quantity of retained biomass is limited by volume exchange. Therefore, the development of continuous flow reactors (CFRs) has come to be regarded as a more competitive option. This is why numerous investigations have been undertaken in recent years in search of different designs of CFR systems that would enable the effective treatment of urban and industrial wastewater, keeping the stability of granular biomass. However, despite these efforts, satisfactory results have yet to be achieved. Consequently, it remains necessary to carry out new technical approaches that would provide more effective and efficient AGS-CFR systems. In particular, it is imperative to develop continuous flow granular systems that can both retain granular biomass and efficiently treat wastewater, obviously with low construction, maintenance and exploitation cost. In this review, we collect the most recent information on different technological approaches aimed at establishing AGS-CFR systems, making possible their upscaling to real plant conditions. We discuss the advantages and disadvantages of these proposals and suggest future trends in the application of aerobic granular systems. Accordingly, we analyze the most significant technical and biological implications of this innovative technology.
Aurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water 2021, 13, 1792 .
AMA StyleAurora Rosa-Masegosa, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Massimiliano Fenice, Susanna Gorrasi, Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water. 2021; 13 (13):1792.
Chicago/Turabian StyleAurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. 2021. "New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects." Water 13, no. 13: 1792.
Enterobacteriaceae is present in various niches worldwide (i.e., the gastrointestinal tracts of animals, clinical specimens, and diverse environments) and hosts some well-known pathogens (i.e., salmonellas, shigellas and pathogenic coliforms). No investigation has focused on its occurrence in marine salterns, and it is not clear if these hypersaline environments could be a reservoir for these bacteria including some potentially harmful members. In this study, a two-year metabarcoding survey was carried out on samples collected from different ponds of the “Saline di Tarquinia” salterns and the nearby coastal waters. Enterobacteriaceae was recorded almost constantly in the seawaters feeding the saltern. Its abundance was generally higher in the sea than in the ponds, probably due to the higher anthropic impact. The same trend was evidenced for the key genus (Escherichia/Shigella) and OTU (OTU 5) of the Enterobacteriaceae community. Various parameters affected taxon/OTU abundance: Enterobacteriaceae, Escherichia/Shigella and OTU5 decreased with increasing salinity and rains; moreover, Escherichia/Shigella and OTU 5 were higher in autumn than in spring. Although Enterobacteriaceae did not seem to find the most favourable conditions for a high-abundance persistence in the saltern environment, it did not disappear. These observations suggested this environment as a potential reservoir for bacteria with possible important health implications.
Susanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water 2021, 13, 1443 .
AMA StyleSusanna Gorrasi, Marcella Pasqualetti, Andrea Franzetti, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez, Barbara Muñoz-Palazon, Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water. 2021; 13 (11):1443.
Chicago/Turabian StyleSusanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. 2021. "Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone." Water 13, no. 11: 1443.
The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during wastewater treatment leads to concerns about whether this process may represent a focal point for the transmission of COVID-19. An epidemiological analysis, based on a COVID-19 IgG/IgM Rapid Test Cassette, performed on 134 wastewater workers from 59 wastewater treatment plants from the province of Granada (Spain) showed a seroprevalence of 8.95% in IgG for SARS-CoV-2, which is similar to the incidence rate found for the general population of the province (9.6%; 95%CI = 7.2–12.8). These findings suggests that current safety measures are sufficient for the protection of workers against SARS-CoV-2.
B. Muñoz-Palazon; P. R. Bouzas; J. González-López; M. Manzanera. Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study. International Journal of Water Resources Development 2021, 1 -10.
AMA StyleB. Muñoz-Palazon, P. R. Bouzas, J. González-López, M. Manzanera. Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study. International Journal of Water Resources Development. 2021; ():1-10.
Chicago/Turabian StyleB. Muñoz-Palazon; P. R. Bouzas; J. González-López; M. Manzanera. 2021. "Transmission of SARS-CoV-2 associated with wastewater treatment: a seroprevalence study." International Journal of Water Resources Development , no. : 1-10.
Two aerobic granular sludge (AGS) sequential batch reactors were operated at a mild (15 °C) temperature for 180 days. One of those bioreactors was exposed to a mixture of diclofenac, naproxen, trimethoprim, and carbamazepine. The AGS system, operating under pressure from emerging contaminants, showed a decrease in COD, BOD5, and TN removal capacity, mainly observed during the first 100 days, in comparison with the removal ratios detected in the control bioreactor. After an acclimatisation period, the removal reached high-quality effluent for COD and TN, close to 95% and 90%, respectively. In the steady-state period, trimethoprim and diclofenac were successfully removed with values around 50%, while carbamazepine and naproxen were more recalcitrant. The dominant bacterial OTUs were affected by the presence of a mixture of pharmaceutical compounds, under which the dominant phylotypes changed to OTUs classified among the Pseudomonas, Gemmobacter, and Comamonadaceae. The RT-qPCR and qPCR results showed the deep effects of pharmaceutical compounds on the number of copies of target genes. Statistical analyses allowed for linking the total and active microbial communities with the physico-chemical performance, describing the effects of pharmaceutical compounds in pollution degradation, as well as the successful adaptation of the system to treat wastewater in the presence of toxic compounds.
Barbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics 2021, 9, 93 .
AMA StyleBarbara Muñoz-Palazon, Aurora Rosa-Masegosa, Miguel Hurtado-Martinez, Alejandro Rodriguez-Sanchez, Alexander Link, Ramiro Vilchez-Vargas, Alejandro Gonzalez-Martinez, Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics. 2021; 9 (5):93.
Chicago/Turabian StyleBarbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. 2021. "Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds." Toxics 9, no. 5: 93.
Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denitrificans Z1, and P. fluorescens PSC26, previously reported as denitrifying microorganisms carrying the nosZ gene. Pseudomonas denitrificans Z1 produced fluffy, low-density granules, with a decantation speed below 10 m h−1. However, P. fluorescens PSC26, P. stutzeri I1, and P. fluorescens 376 formed stable granules, with mean size from 7 to 15 mm, related to the strain and carbon concentration. P. stutzeri I1 and P. fluorescens 376 removed nitrate efficiently with a ratio in the range of 96%, depending on the source and concentration of organic matter. Therefore, the findings suggest that the inoculation of GSBR systems with denitrifying strains of Pseudomonas spp. containing the nosZ gene enables the formation of stable granules, the efficient removal of nitrate, and the transformation of nitrate into nitrogen gas, a result of considerable environmental interest to avoid the generation of nitrous oxide.
Miguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water 2021, 13, 1119 .
AMA StyleMiguel Hurtado-Martinez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maximino Manzanera, Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water. 2021; 13 (8):1119.
Chicago/Turabian StyleMiguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. 2021. "Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors." Water 13, no. 8: 1119.
A pilot-scale anammox biofilter was set up and operated using reject water in order to investigate its nitrogen removal performance and the microbial community present at different stages of operation of this technology. A novel material “Filtralite” was used a support in order to achieve shorter start-up periods and obtain higher nitrogen load removal than other anammox technologies. Physico-chemical analysis demonstrated that nitrogen removal efficiency was higher than 70% from operational Day 100, reaching steady-stable total nitrogen removal and NH4+-N of 82% and 91%, respectively, this results suggest the robustness and adaptability of the technology to withstand changing nitrogen loads in the influent. Molecular analysis showed the presence of Nitrosomonas and Candidatus Brocadiales with relative abundance on different key days at around 2.5% of total relative abundance, although other phylotypes involved in the nitrogen cycle were also found. RT-PCR analysis showed that the pre-seed Filtralite used promoted the occurrence of anammox bacteria and other ammonia-oxidizing bacteria. The eukaryotic community oscillated greatly during operation and did not show any clear pattern. Both Prokarya and Eukarya showed high diversity and evenness, attributes which were very stable during operation. The biofilter performed robustly, despite changes in influent and nitrogen load, validating its suitability for treating industrial or urban effluent with high nitrogen loading in order to avoid damages in the environment in the save-cost way.
Maria Jesus Garcia-Ruiz; Barbara Muñoz-Palazon; Jesus Gonzalez-Lopez; Francisco Osorio. Performance and microbial community structure of an anammox biofilter treating real wastewater from a sludge return. Journal of Environmental Chemical Engineering 2021, 9, 105211 .
AMA StyleMaria Jesus Garcia-Ruiz, Barbara Muñoz-Palazon, Jesus Gonzalez-Lopez, Francisco Osorio. Performance and microbial community structure of an anammox biofilter treating real wastewater from a sludge return. Journal of Environmental Chemical Engineering. 2021; 9 (3):105211.
Chicago/Turabian StyleMaria Jesus Garcia-Ruiz; Barbara Muñoz-Palazon; Jesus Gonzalez-Lopez; Francisco Osorio. 2021. "Performance and microbial community structure of an anammox biofilter treating real wastewater from a sludge return." Journal of Environmental Chemical Engineering 9, no. 3: 105211.
The aim of this work was to study the operational performance and the microbial community dynamics during the start-up of ANITATMMox technology implemented at full-scale wastewater treatment plant in Finland to treat reject water from anaerobic digesters. The average ammonium removal in the studied setup reached around 90%, withstanding ammonium loads up to 0.13 g N m−2h−1. The nitrite concentration in the effluent did not exceed 10 mg L−1, and there was a slight accumulation of NO3−-N during the operation which was controlled. Thus, the result showed a robust success to high ammonium loading in presence of organic matter. The sequencing showed a heterogeneous microbial population where Methanosaeta, WCHA1-57 genus, Sphingobacteriia, Chlorobia and diverse unknown fungi were found as dominant phylotypes. Moreover, members of the Brocadiaceae family were dominant in the adhered biomass, mostly represented by Candidatus Scalindua, rarely reported in WWTPs. Overall, the results demonstrated a drastic effect of region-specific operational conditions on carrier biofilm microbial communities as it was demonstrated by the microbial studies.
A. González-Martínez; B. Muñoz-Palazon; A. Kruglova; M. Vilpanen; A. Kuokkanen; A. Mikola; M. Heinonen. Performance and microbial community structure of a full-scale ANITATMMox bioreactor for treating reject water located in Finland. Chemosphere 2021, 271, 129526 .
AMA StyleA. González-Martínez, B. Muñoz-Palazon, A. Kruglova, M. Vilpanen, A. Kuokkanen, A. Mikola, M. Heinonen. Performance and microbial community structure of a full-scale ANITATMMox bioreactor for treating reject water located in Finland. Chemosphere. 2021; 271 ():129526.
Chicago/Turabian StyleA. González-Martínez; B. Muñoz-Palazon; A. Kruglova; M. Vilpanen; A. Kuokkanen; A. Mikola; M. Heinonen. 2021. "Performance and microbial community structure of a full-scale ANITATMMox bioreactor for treating reject water located in Finland." Chemosphere 271, no. : 129526.
Granular aerobic sludge systems have been a very efficient technology in urban and industrial wastewater treatment. In this research, a novel modification of aerobic granular sludge technology was developed for the treatment of nitrate-polluted groundwater, adding very low concentrations of a solution based on carbon and oligoelements in the groundwater to promote the growth of denitrifying microorganisms, avoiding expensive technologies to supply drinking water in small urban nuclei. The denitrification process was successfully reached at 0.15 g C2H3NaO2 L−1, meeting the Nitrate Directive of Europe for drinking water. The granular biomass was compact and dense with average values of mean size and settling velocity of 4.0 mm and 40 mh−1, respectively. The prokaryotic and eukaryotic communities were studied by massive parallel sequencing techniques. The dominant prokaryotic phylotypes were related to influent composition, belonging to Comamonadaceae, Rhizobiales, Acinetobacter and Pseudomonas. The dominant eukaryotic phylotype was affiliated to Haematococcus microalgae. The diversity and evenness were high, regardless of influent composition. This study demonstrates support for the innovation of aerobic granular sludge technology application in terms of performance, operation, granular maturation and stability, as well as the role of denitrifying microorganisms to implement a low-cost, easy-to-use and maintain, environmental-friendly drinking water technology for rural populations.
Miguel Hurtado-Martinez; Bárbara Muñoz-Palazon; Virginia María Robles-Arenas; Alejandro Gonzalez-Martinez; Jesús Gonzalez-Lopez. Biological nitrate removal from groundwater by an aerobic granular technology to supply drinking water at pilot-scale. Journal of Water Process Engineering 2020, 40, 101786 .
AMA StyleMiguel Hurtado-Martinez, Bárbara Muñoz-Palazon, Virginia María Robles-Arenas, Alejandro Gonzalez-Martinez, Jesús Gonzalez-Lopez. Biological nitrate removal from groundwater by an aerobic granular technology to supply drinking water at pilot-scale. Journal of Water Process Engineering. 2020; 40 ():101786.
Chicago/Turabian StyleMiguel Hurtado-Martinez; Bárbara Muñoz-Palazon; Virginia María Robles-Arenas; Alejandro Gonzalez-Martinez; Jesús Gonzalez-Lopez. 2020. "Biological nitrate removal from groundwater by an aerobic granular technology to supply drinking water at pilot-scale." Journal of Water Process Engineering 40, no. : 101786.
Biofouling significantly reduces the performance efficiency of membrane bioreactors due to the colonization and accumulation of a matrix of microorganisms. In order to investigate the effect of ultrasonic frequency on biofilm formation and bacterial ecology at different frequencies, four microfiltration membrane modules were subjected at 0, 20, 30 and 40 kHz in pilot-scales. The results of massive parallel sequencing showed that the application of ultrasonic frequency significantly affected the bacterial population in biofouling and modifies the species richness even at low frequency. This research reveals that the application of ultrasonic frequencies avoids the colonization of Acinetobacter genus, which is considered an initial colonizer of biofouling and clogging in the submerged membrane reactor, fact that caused problems in full-scale wastewater treatment plant. The promotion of Gordonia genus was observed during the application of any ultrasounds frequencies (20,30 and 40 Hz). The compositional statistics data exposed the similarity of bacterial communities under medium-high ultrasonic frequency, while those great distances were found in both low and no-ultrasounds frequencies apply. These results suggest that the application of ultrasounds in membrane bioreactor for treating wastewater could be effective in the potential reduction of biofouling formation in order to improve the to improve the useful life of this system with short periods of application of ultrasound.
Alfonso Rodríguez-Calvo; Jesus Gonzalez-Lopez; Luz Marina Ruiz; Miguel Ángel Gómez-Nieto; Barbara Muñoz-Palazon. Effect of ultrasonic frequency on the bacterial community structure during biofouling formation in microfiltration membrane bioreactors for wastewater treatment. International Biodeterioration & Biodegradation 2020, 155, 105102 .
AMA StyleAlfonso Rodríguez-Calvo, Jesus Gonzalez-Lopez, Luz Marina Ruiz, Miguel Ángel Gómez-Nieto, Barbara Muñoz-Palazon. Effect of ultrasonic frequency on the bacterial community structure during biofouling formation in microfiltration membrane bioreactors for wastewater treatment. International Biodeterioration & Biodegradation. 2020; 155 ():105102.
Chicago/Turabian StyleAlfonso Rodríguez-Calvo; Jesus Gonzalez-Lopez; Luz Marina Ruiz; Miguel Ángel Gómez-Nieto; Barbara Muñoz-Palazon. 2020. "Effect of ultrasonic frequency on the bacterial community structure during biofouling formation in microfiltration membrane bioreactors for wastewater treatment." International Biodeterioration & Biodegradation 155, no. : 105102.
In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an important role in the microbial dynamics in wastewater treatment systems and the biosafety of effluents. In this work, the viral members present in influent wastewater, mixed liquor (aerobic bioreactor), excess sludge, and effluent water of a conventional activated sludge system for the treatment of urban wastewater were identified. Viral members were observed by transmission electron microscopy and studied through next-generation sequencing studies. The results showed the dominance of bacteriophages in the viral community in all samples, with the dominant viral phylotype classified as Escherichia coli O157 typing phage 7. Moreover, different human viruses, such as Cynomolgus cytomegalovirus and Gammaherpesvirus, were also detected.
Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water 2020, 12, 2316 .
AMA StyleCristina García-Fontana, Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maria Vela-Cano, Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water. 2020; 12 (8):2316.
Chicago/Turabian StyleCristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. 2020. "Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain." Water 12, no. 8: 2316.
Three bioreactors were inoculated with Polar Arctic Circle-activated sludge, started-up and operated for 150 days at 8, 15 and 26 °C. Removal performances and granular conformation were similar at steady-state, but higher stability from start-up was found when operating at 8 °C. Important changes in the eukaryotic and prokaryotic populations caused by operational temperature were observed, being fungi dominant at 8 °C and 15 °C, while that ciliated organisms were found at 26 °C. The qPCR results showed higher copies of bacteria, and nitrifiers and denitrifying bacteria at cold temperature. The emission of nitrous oxide was linked directly with temperature and the involved microorganisms. This study represents a proof of concept in performance, greenhouse gas emission, granular formation and the role of the Polar Arctic Circle microbial population in AGS technology under different temperatures with the aim to understand the effect of seasonal o daily changes for implementation of AGS at full-scale.
Bárbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Francisco Santana; Jesus Gonzalez-Lopez; Leoni Mack; Alejandro Gonzalez-Martinez. Polar Arctic Circle biomass enhances performance and stability of aerobic granular sludge systems operated under different temperatures. Bioresource Technology 2019, 300, 122650 .
AMA StyleBárbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Miguel Hurtado-Martinez, Francisco Santana, Jesus Gonzalez-Lopez, Leoni Mack, Alejandro Gonzalez-Martinez. Polar Arctic Circle biomass enhances performance and stability of aerobic granular sludge systems operated under different temperatures. Bioresource Technology. 2019; 300 ():122650.
Chicago/Turabian StyleBárbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Francisco Santana; Jesus Gonzalez-Lopez; Leoni Mack; Alejandro Gonzalez-Martinez. 2019. "Polar Arctic Circle biomass enhances performance and stability of aerobic granular sludge systems operated under different temperatures." Bioresource Technology 300, no. : 122650.
The present work aims to use aerobic granular sludge technology for the treatment of wastewater containing high organic matter loads and a mixture of phenolic compounds normally present in olive washing water. The physicochemical performance of five bioreactors treating different concentrations of mixture of phenolic acid was monitored to observe the response of the systems. The bioreactors that operated at 50, 100 and 300 mg L−1 did not show relevant changes in terms of performance and granules properties, showing high ratio of phenolic compound removal ratio. However, the bioreactors operated with high phenolic compound concentrations showed low rates of organic matter, nitrogen and phenolic acid removal. In the same way, high concentrations of phenolic compounds determined the disintegration of the granular biomass. Next-generation sequencing studies showed a stable community structure in the bioreactors operating with 50, 100 and 300 mg L−1 of phenolic acids, with the genera Lampropedia and Arenimonas, family Xanthobacteraceae and Fungi Pezizomycotina as the dominant phylotypes. Conversely, the reactors operated at 500 and 600 mg L−1 of phenolic substances promoted the proliferation of Oligohymenophorea ciliates. Thus, this study suggests that aerobic granular sludge technology could be useful for the treatment of wastewaters such as olive washing water.
Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Ines Manuel de Castro; Belén Juarez-Jimenez; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez. Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations. Journal of Hazardous Materials 2019, 376, 58 -67.
AMA StyleBarbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Miguel Hurtado-Martinez, Ines Manuel de Castro, Belén Juarez-Jimenez, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez. Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations. Journal of Hazardous Materials. 2019; 376 ():58-67.
Chicago/Turabian StyleBarbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Ines Manuel de Castro; Belén Juarez-Jimenez; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez. 2019. "Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations." Journal of Hazardous Materials 376, no. : 58-67.
A lab-scale partial nitritation SBR was operated at 11 °C for 300 days used for the treatment of high-ammonium wastewater, which was inoculated with activated sludge from Rovaniemi WWTP (located in Polar Arctic Circle) in order to evaluate the influence the temperature on the performance, stability and dynamics of its microbial community. The partial nitritation achieved steady-state long-term operation and granulation process was not affected despite the low temperature and high ammonia concentration. The steady conditions were reached after 60 days of operation where the granular biomass was fully-formed and the 50%–50% of ammonium-nitrite effluent was successful achieved. Inoculation with cold adapted inoculum showed to yield bigger, denser granules with faster start-up without necessity of low temperature adaptation period. Next-generation sequences techniques showed that Trichosporonaceae and Xanthomonadaceae were the dominant OTUs in the mature granules. Our study could be useful in the implementation of full-scale partial nitritation reactors in cold regions such as Nordic countries for treating wastewater with high concentration of ammonium.
Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Paula Maza-Marquez; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature. Chemosphere 2019, 225, 73 -82.
AMA StyleAlejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Miguel Hurtado-Martinez, Paula Maza-Marquez, Jesus Gonzalez-Lopez, Riku Vahala, Alejandro Gonzalez-Martinez. Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature. Chemosphere. 2019; 225 ():73-82.
Chicago/Turabian StyleAlejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Paula Maza-Marquez; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. 2019. "Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature." Chemosphere 225, no. : 73-82.
Two pilot-scale hybrid moving bed biofilm reactor-membrane bioreactors were operated in parallel for the treatment of salinity-amended urban wastewater under 6 hours of hydraulic retention time and 2500 mg L−1 total solids concentration. Two salinity conditions were tested: the constant salinity of 6.5 mS cm−1 electric conductivity (3.6 g L−1 NaCl) and the tidal-like variable salinity with maximum 6.5 mS cm−1 electric conductivity. An investigation was developed on the biofouling produced on the ultrafiltration membrane surface evaluating its bacterial community structure and its potential function in the fouling processes. The results showed that biofouling was clearly affected by salinity scenarios in terms of α-diversity and β-diversity and bacterial community structure, which confirms lower bacterial diversity under variable salinity conditions with Rhodanobacter and Dyella as dominant phylotypes. Microorganisms identified as bio-mineral formers belonged to genera Bacillus, Citrobacter, and Brevibacterium. These findings will be of help for the prevention and control of biofouling in saline wastewater treatment systems.
Alejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water 2018, 10, 1133 .
AMA StyleAlejandro Rodriguez-Sanchez, Juan Carlos Leyva-Diaz, Barbara Muñoz-Palazon, Maria Angustias Rivadeneyra, Miguel Hurtado-Martinez, Daniel Martin-Ramos, Alejandro Gonzalez-Martinez, Jose Manuel Poyatos, Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water. 2018; 10 (9):1133.
Chicago/Turabian StyleAlejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. 2018. "Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration." Water 10, no. 9: 1133.
The aim of this work was to study the performance and microbial community structure of a polar Arctic Circle aerobic granular sludge (AGS) system operating at low temperature. Thus, an AGS bioreactor was operated at 7, 5 and 3 °C of temperature using a cold-adapted sludge from Lapland. At 5 °C, it yielded acceptable conversion rates, in terms of nitrogen, phosphorous, and organic matter. However, under 3 °C a negligible nitrogen and phosphorous removal performance was observed. Below 5 °C, scanning electron microscopy studies showed a wispy, non-dense and irregular granular structure with a strong outgrowth of filamentous. Moreover, Illumina next-generation sequencing showed a heterogeneous microbial population where SM1K20 (Archaea), Trichosporon domesticum (Fungus), and Zooglea, Arcobacter and Acinetobacter (Bacteria) were the dominant phylotypes. Our study suggests that AGS technologies inoculated with North Pole sludge could be operated, in cold regions for a period longer than 3 months (winter season) under 5 °C of water temperature.
Alejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Paula Maza-Márquez; Alejandro Rodriguez-Sanchez; Jesus Gonzalez-Lopez; Riku Vahala. Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature. Bioresource Technology 2018, 256, 22 -29.
AMA StyleAlejandro Gonzalez-Martinez, Barbara Muñoz Palazon, Paula Maza-Márquez, Alejandro Rodriguez-Sanchez, Jesus Gonzalez-Lopez, Riku Vahala. Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature. Bioresource Technology. 2018; 256 ():22-29.
Chicago/Turabian StyleAlejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Paula Maza-Márquez; Alejandro Rodriguez-Sanchez; Jesus Gonzalez-Lopez; Riku Vahala. 2018. "Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature." Bioresource Technology 256, no. : 22-29.
Seven full-scale biological wastewater treatment systems located in the Polar Arctic Circle region in Finland were investigated to determine their Archaea, Bacteria and Fungi community structure, and their relationship with the operational conditions of the bioreactors by the means of quantitative PCR, massive parallel sequencing and multivariate redundancy analysis. The results showed dominance of Archaea and Bacteria members in the bioreactors. The activated sludge systems showed strong selection of Bacteria but not for Archaea and Fungi, as suggested by diversity analyses. Core OTUs in influent and bioreactors were classified as Methanobrevibacter, Methanosarcina, Terrestrial Group Thaumarchaeota and unclassified Euryarchaeota member for Archaea; Trichococcus, Leptotrichiaceae and Comamonadaceae family, and Methylorosula for Bacteria and Trichosporonaceae family for Fungi. All influents shared core OTUs in all domains, but in bioreactors this did not occur for Bacteria. Oligotype structure of core OTUs showed several ubiquitous Fungi oligotypes as dominant in sewage and bioreactors. Multivariate redundancy analyses showed that the majority of core OTUs were related to organic matter and nutrients removal. Also, there was evidence of competition among Archaea and Fungi core OTUs, while all Bacteria OTUs were positively correlated among them. The results obtained highlighted interesting features of extremely cold temperature bioreactors.
Alejandro Gonzalez-Martinez; Maija Sihvonen; Barbara Muñoz Palazon; Alejandro Rodriguez-Sanchez; Anna Mikola; Riku Vahala. Microbial ecology of full-scale wastewater treatment systems in the Polar Arctic Circle: Archaea, Bacteria and Fungi. Scientific Reports 2018, 8, 1 -11.
AMA StyleAlejandro Gonzalez-Martinez, Maija Sihvonen, Barbara Muñoz Palazon, Alejandro Rodriguez-Sanchez, Anna Mikola, Riku Vahala. Microbial ecology of full-scale wastewater treatment systems in the Polar Arctic Circle: Archaea, Bacteria and Fungi. Scientific Reports. 2018; 8 (1):1-11.
Chicago/Turabian StyleAlejandro Gonzalez-Martinez; Maija Sihvonen; Barbara Muñoz Palazon; Alejandro Rodriguez-Sanchez; Anna Mikola; Riku Vahala. 2018. "Microbial ecology of full-scale wastewater treatment systems in the Polar Arctic Circle: Archaea, Bacteria and Fungi." Scientific Reports 8, no. 1: 1-11.
An aerobic granular sludge system has been started-up and operated at 7°C temperature using cold-adapted activated sludge as inoculum. The system could form granular biomass due to batch operation allowing for just 5-3min of biomass sedimentation. Scanning electron microscopy showed that fungi helped in the granular biomass formation in the early stages of the granule formation. The removal performance of the system was of 92-95% in BOD5, 75-80% in COD, 70-76% in total nitrogen and 50-60% in total phosphorous. The bacterial community structure from cold-adapted activated sludge changed during the operational time, leading to a final configuration dominated by Microbacteriaceae members Microbacterium and Leucobacter, which were strongly correlated to biomass settling velocity and bioreactor performance, as suggested by multivariate redundancy analyses. This experiment showed that aerobic granular sludge systems could be successfully started-up and operated, with high performance, under low operational temperatures when using cold-adapted biomass as inoculum.
Alejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Alejandro Rodriguez-Sanchez; Paula Maza-Márquez; Anna Mikola; Jesus Gonzalez-Lopez; Riku Vahala. Start-up and operation of an aerobic granular sludge system under low working temperature inoculated with cold-adapted activated sludge from Finland. Bioresource Technology 2017, 239, 180 -189.
AMA StyleAlejandro Gonzalez-Martinez, Barbara Muñoz Palazon, Alejandro Rodriguez-Sanchez, Paula Maza-Márquez, Anna Mikola, Jesus Gonzalez-Lopez, Riku Vahala. Start-up and operation of an aerobic granular sludge system under low working temperature inoculated with cold-adapted activated sludge from Finland. Bioresource Technology. 2017; 239 ():180-189.
Chicago/Turabian StyleAlejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Alejandro Rodriguez-Sanchez; Paula Maza-Márquez; Anna Mikola; Jesus Gonzalez-Lopez; Riku Vahala. 2017. "Start-up and operation of an aerobic granular sludge system under low working temperature inoculated with cold-adapted activated sludge from Finland." Bioresource Technology 239, no. : 180-189.
Autotrophic nitrogen removal systems have been implemented at full‐scale and provide an efficient way for nitrogen removal from industrial and urban wastewaters. Our study present qualitative and quantitative analysis of archaeal and bacterial amoA genes and Candidatus Brocadiales bacteria analyzed in six full‐scale autotrophic nitrogen removal bioreactors. The results showed that ammonium oxidizing bacteria (AOB) were detected in all bioreactors. However, ammonium oxidizing archaea (AOA) were detected only in the non‐aerated technologies. Conversely, different Candidatus Brocadiales phylotypes appeared due to differences in influent wastewater composition and hydraulic retention time (HRT). In the same terms multivariate redundancy analysis confirmed that AOA was positively correlated with temperature, ammonium concentration and low HRT. However, AOB population was positively correlated with pH, temperature, and dissolved oxygen concentration. Our data suggested a correlation between the microorganisms involved in the nitrogen removal performance and the operational conditions in the different full‐scale bioreactors. © 2017 American Institute of Chemical Engineers AIChE J, 64: 457–467, 2018
Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Antonio Castellano-Hinojosa; Jesus Gonzalez-Lopez; Mark C. M. Van Loosdrecth; Riku Vahala; Alejandro Gonzalez-Martinez. Quantitative and qualitative studies of microorganisms involved in full-scale autotrophic nitrogen removal performance. AIChE Journal 2017, 64, 457 -467.
AMA StyleBarbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Antonio Castellano-Hinojosa, Jesus Gonzalez-Lopez, Mark C. M. Van Loosdrecth, Riku Vahala, Alejandro Gonzalez-Martinez. Quantitative and qualitative studies of microorganisms involved in full-scale autotrophic nitrogen removal performance. AIChE Journal. 2017; 64 (2):457-467.
Chicago/Turabian StyleBarbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Antonio Castellano-Hinojosa; Jesus Gonzalez-Lopez; Mark C. M. Van Loosdrecth; Riku Vahala; Alejandro Gonzalez-Martinez. 2017. "Quantitative and qualitative studies of microorganisms involved in full-scale autotrophic nitrogen removal performance." AIChE Journal 64, no. 2: 457-467.
Alejandro Rodriguez-Sanchez; Anna Mikola; Barbara Muñoz Palazon; Riku Vahala; Alejandro Gonzalez-Martinez. Performance and bacterial community structure of a submerged biofilter subjected to high ammonium and high organic carbon concentrations. International Biodeterioration & Biodegradation 2016, 115, 224 -233.
AMA StyleAlejandro Rodriguez-Sanchez, Anna Mikola, Barbara Muñoz Palazon, Riku Vahala, Alejandro Gonzalez-Martinez. Performance and bacterial community structure of a submerged biofilter subjected to high ammonium and high organic carbon concentrations. International Biodeterioration & Biodegradation. 2016; 115 ():224-233.
Chicago/Turabian StyleAlejandro Rodriguez-Sanchez; Anna Mikola; Barbara Muñoz Palazon; Riku Vahala; Alejandro Gonzalez-Martinez. 2016. "Performance and bacterial community structure of a submerged biofilter subjected to high ammonium and high organic carbon concentrations." International Biodeterioration & Biodegradation 115, no. : 224-233.
Partial-nitritation processes are used for the biological treatment of high nitrogen-low organic carbon effluents, such as anaerobic digestion reject water. The release of certain products generated during the anaerobic digestion process, such as amino acids, could potentially reduce the performance of these partial-nitritation bioprocesses. To investigate this, four partial-nitritation biofilters were subjected to continuous addition of 0, 150, 300 and 500 mg L−1 cysteine amino acid in their influents. The addition of the amino acid had an impact over the performance of the partial-nitritation process and the bacterial community dynamics of the systems analyzed. Ammonium oxidation efficiency decreased with the addition of the amino acid, and a net nitrogen elimination occurred in presence of cysteine through the operation period. Bacterial community dynamics showed a decrease of Nitrosomonas species and a proliferation of putative heterotrophs with nitrification capacity, such as Pseudomonas, or denitrification capacity, such as Denitrobacter or Alicycliphilus. The addition of cysteine irreversible affected the bioreactors, which could not achieve the performance obtained before the addition of the amino acid. A mathematical predictive equation of the process performance depending on cysteine concentration added and operational time under such concentration was developed. This article is protected by copyright. All rights reserved.
Alejandro Rodriguez‐Sanchez; Barbara Munoz‐Palazon; Paula Maza‐Marquez; Jesus Gonzalez‐Lopez; Riku Vahala; Alejandro Gonzalez‐Martinez. Process performance and bacterial community dynamics of partial-nitritation biofilters subjected to different concentrations of cysteine amino acid. Biotechnology Progress 2016, 32, 1254 -1263.
AMA StyleAlejandro Rodriguez‐Sanchez, Barbara Munoz‐Palazon, Paula Maza‐Marquez, Jesus Gonzalez‐Lopez, Riku Vahala, Alejandro Gonzalez‐Martinez. Process performance and bacterial community dynamics of partial-nitritation biofilters subjected to different concentrations of cysteine amino acid. Biotechnology Progress. 2016; 32 (5):1254-1263.
Chicago/Turabian StyleAlejandro Rodriguez‐Sanchez; Barbara Munoz‐Palazon; Paula Maza‐Marquez; Jesus Gonzalez‐Lopez; Riku Vahala; Alejandro Gonzalez‐Martinez. 2016. "Process performance and bacterial community dynamics of partial-nitritation biofilters subjected to different concentrations of cysteine amino acid." Biotechnology Progress 32, no. 5: 1254-1263.