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Dr. Alejandro Gonzalez-Martinez
University of Granada, Granada, Spain

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0 Biotechnology
0 Molecular Biology
0 Next-generation sequencing
0 biological wastewater treatment
0 Applied Microbiology

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Next-generation sequencing
biological wastewater treatment
Biotechnology

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Review
Published: 29 June 2021 in Water
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Aerobic granular sludge (AGS) comprises an aggregation of microbial cells in a tridimensional matrix, which is able to remove carbon, nitrogen and phosphorous as well as other pollutants in a single bioreactor under the same operational conditions. During the past decades, the feasibility of implementing AGS in wastewater treatment plants (WWTPs) for treating sewage using fundamentally sequential batch reactors (SBRs) has been studied. However, granular sludge technology using SBRs has several disadvantages. For instance, it can present certain drawbacks for the treatment of high flow rates; furthermore, the quantity of retained biomass is limited by volume exchange. Therefore, the development of continuous flow reactors (CFRs) has come to be regarded as a more competitive option. This is why numerous investigations have been undertaken in recent years in search of different designs of CFR systems that would enable the effective treatment of urban and industrial wastewater, keeping the stability of granular biomass. However, despite these efforts, satisfactory results have yet to be achieved. Consequently, it remains necessary to carry out new technical approaches that would provide more effective and efficient AGS-CFR systems. In particular, it is imperative to develop continuous flow granular systems that can both retain granular biomass and efficiently treat wastewater, obviously with low construction, maintenance and exploitation cost. In this review, we collect the most recent information on different technological approaches aimed at establishing AGS-CFR systems, making possible their upscaling to real plant conditions. We discuss the advantages and disadvantages of these proposals and suggest future trends in the application of aerobic granular systems. Accordingly, we analyze the most significant technical and biological implications of this innovative technology.

ACS Style

Aurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water 2021, 13, 1792 .

AMA Style

Aurora Rosa-Masegosa, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Massimiliano Fenice, Susanna Gorrasi, Jesus Gonzalez-Lopez. New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects. Water. 2021; 13 (13):1792.

Chicago/Turabian Style

Aurora Rosa-Masegosa; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Massimiliano Fenice; Susanna Gorrasi; Jesus Gonzalez-Lopez. 2021. "New Advances in Aerobic Granular Sludge Technology Using Continuous Flow Reactors: Engineering and Microbiological Aspects." Water 13, no. 13: 1792.

Journal article
Published: 21 May 2021 in Water
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Enterobacteriaceae is present in various niches worldwide (i.e., the gastrointestinal tracts of animals, clinical specimens, and diverse environments) and hosts some well-known pathogens (i.e., salmonellas, shigellas and pathogenic coliforms). No investigation has focused on its occurrence in marine salterns, and it is not clear if these hypersaline environments could be a reservoir for these bacteria including some potentially harmful members. In this study, a two-year metabarcoding survey was carried out on samples collected from different ponds of the “Saline di Tarquinia” salterns and the nearby coastal waters. Enterobacteriaceae was recorded almost constantly in the seawaters feeding the saltern. Its abundance was generally higher in the sea than in the ponds, probably due to the higher anthropic impact. The same trend was evidenced for the key genus (Escherichia/Shigella) and OTU (OTU 5) of the Enterobacteriaceae community. Various parameters affected taxon/OTU abundance: Enterobacteriaceae, Escherichia/Shigella and OTU5 decreased with increasing salinity and rains; moreover, Escherichia/Shigella and OTU 5 were higher in autumn than in spring. Although Enterobacteriaceae did not seem to find the most favourable conditions for a high-abundance persistence in the saltern environment, it did not disappear. These observations suggested this environment as a potential reservoir for bacteria with possible important health implications.

ACS Style

Susanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water 2021, 13, 1443 .

AMA Style

Susanna Gorrasi, Marcella Pasqualetti, Andrea Franzetti, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez, Barbara Muñoz-Palazon, Massimiliano Fenice. Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone. Water. 2021; 13 (11):1443.

Chicago/Turabian Style

Susanna Gorrasi; Marcella Pasqualetti; Andrea Franzetti; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez; Barbara Muñoz-Palazon; Massimiliano Fenice. 2021. "Persistence of Enterobacteriaceae Drawn into a Marine Saltern (Saline di Tarquinia, Italy) from the Adjacent Coastal Zone." Water 13, no. 11: 1443.

Journal article
Published: 23 April 2021 in Toxics
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Two aerobic granular sludge (AGS) sequential batch reactors were operated at a mild (15 °C) temperature for 180 days. One of those bioreactors was exposed to a mixture of diclofenac, naproxen, trimethoprim, and carbamazepine. The AGS system, operating under pressure from emerging contaminants, showed a decrease in COD, BOD5, and TN removal capacity, mainly observed during the first 100 days, in comparison with the removal ratios detected in the control bioreactor. After an acclimatisation period, the removal reached high-quality effluent for COD and TN, close to 95% and 90%, respectively. In the steady-state period, trimethoprim and diclofenac were successfully removed with values around 50%, while carbamazepine and naproxen were more recalcitrant. The dominant bacterial OTUs were affected by the presence of a mixture of pharmaceutical compounds, under which the dominant phylotypes changed to OTUs classified among the Pseudomonas, Gemmobacter, and Comamonadaceae. The RT-qPCR and qPCR results showed the deep effects of pharmaceutical compounds on the number of copies of target genes. Statistical analyses allowed for linking the total and active microbial communities with the physico-chemical performance, describing the effects of pharmaceutical compounds in pollution degradation, as well as the successful adaptation of the system to treat wastewater in the presence of toxic compounds.

ACS Style

Barbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics 2021, 9, 93 .

AMA Style

Barbara Muñoz-Palazon, Aurora Rosa-Masegosa, Miguel Hurtado-Martinez, Alejandro Rodriguez-Sanchez, Alexander Link, Ramiro Vilchez-Vargas, Alejandro Gonzalez-Martinez, Jesus Lopez. Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds. Toxics. 2021; 9 (5):93.

Chicago/Turabian Style

Barbara Muñoz-Palazon; Aurora Rosa-Masegosa; Miguel Hurtado-Martinez; Alejandro Rodriguez-Sanchez; Alexander Link; Ramiro Vilchez-Vargas; Alejandro Gonzalez-Martinez; Jesus Lopez. 2021. "Total and Metabolically Active Microbial Community of Aerobic Granular Sludge Systems Operated in Sequential Batch Reactors: Effect of Pharmaceutical Compounds." Toxics 9, no. 5: 93.

Journal article
Published: 19 April 2021 in Water
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Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denitrificans Z1, and P. fluorescens PSC26, previously reported as denitrifying microorganisms carrying the nosZ gene. Pseudomonas denitrificans Z1 produced fluffy, low-density granules, with a decantation speed below 10 m h−1. However, P. fluorescens PSC26, P. stutzeri I1, and P. fluorescens 376 formed stable granules, with mean size from 7 to 15 mm, related to the strain and carbon concentration. P. stutzeri I1 and P. fluorescens 376 removed nitrate efficiently with a ratio in the range of 96%, depending on the source and concentration of organic matter. Therefore, the findings suggest that the inoculation of GSBR systems with denitrifying strains of Pseudomonas spp. containing the nosZ gene enables the formation of stable granules, the efficient removal of nitrate, and the transformation of nitrate into nitrogen gas, a result of considerable environmental interest to avoid the generation of nitrous oxide.

ACS Style

Miguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water 2021, 13, 1119 .

AMA Style

Miguel Hurtado-Martinez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maximino Manzanera, Jesus Gonzalez-Lopez. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water. 2021; 13 (8):1119.

Chicago/Turabian Style

Miguel Hurtado-Martinez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maximino Manzanera; Jesus Gonzalez-Lopez. 2021. "Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors." Water 13, no. 8: 1119.

Research article
Published: 30 March 2021 in Environmental Science and Pollution Research
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A sequential bed granular bioreactor was adapted to treat nitrate-polluted synthetic groundwater under anaerobic conditions and agitation with denitrification gas, achieving very efficient performance in total nitrogen removal at influent organic carbon concentrations of 1 g L-1 (80–90%) and 0.5 g L-1 (70–80%) sodium acetate, but concentrations below 0.5 g L-1 caused accumulation of nitrite and nitrate and led to system failure (30–40% removal). Biomass size and settling velocity were higher above 0.5 g L-1 sodium acetate. Trichosporonaceae dominated the fungal populations at all times, while a dominance of terrestrial group Thaumarchaeota and Acidovorax at 1 and 0.5 g L-1 passed to a domination of Methanobrevibacter and an unclassified Comamonadaceae clone for NaAc lower than 0.5 g L-1. The results obtained pointed out that the denitrifying granular sludge technology is a feasible solution for the treatment of nitrogen-contaminated groundwater, and that influent organic matter plays an important role on the conformation of microbial communities within it and, therefore, on the overall efficiency of the system.

ACS Style

Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Jesús Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. Evaluating the nitrogen-contaminated groundwater treatment by a denitrifying granular sludge bioreactor: effect of organic matter loading. Environmental Science and Pollution Research 2021, 28, 41351 -41364.

AMA Style

Barbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Miguel Hurtado-Martinez, Jesús Gonzalez-Lopez, Riku Vahala, Alejandro Gonzalez-Martinez. Evaluating the nitrogen-contaminated groundwater treatment by a denitrifying granular sludge bioreactor: effect of organic matter loading. Environmental Science and Pollution Research. 2021; 28 (30):41351-41364.

Chicago/Turabian Style

Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Jesús Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. 2021. "Evaluating the nitrogen-contaminated groundwater treatment by a denitrifying granular sludge bioreactor: effect of organic matter loading." Environmental Science and Pollution Research 28, no. 30: 41351-41364.

Journal article
Published: 31 August 2020 in Water
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In this study, the microbial community of nitrifying activated sludge adapted to Finnish climate conditions was studied to clarify the microbial populations involved in low-temperature nitrification. Microbial community analysis of five full-scale wastewater treatment plants (WWTPs) showed several differences compared to WWTPs from other countries with a similar climate. In particular, very low abundance of ammonium oxidizing bacteria (AOBs) (altogether ˂ 0.25% of total community) as well as typical NOBs (˂0.35%) and a high abundance of orders Cytophagales and Micrococcales was observed in all Finnish WWTPs. To shed light on the importance of autotrophic and heterotrophic nitrifying processes, laboratory studies of activated sludge were carried out with a presence of and a lack of organic carbon in wastewater at 10 ± 1 °C. Two different sludge retention times (SRTs) were compared to determine the effect of this operational parameter on low-temperature nitrogen removal. The important role of previously reported Candidatus Nitrotogaarctica for nitrite oxidizing in cold climate conditions was confirmed in both full-scale and laboratory scale results. Additionally, potential participation of Dokdonella sp. and Flexibacter sp. in nitrogen removal at low-temperatures is proposed. Operation at SRT of 100 days demonstrated more stable and efficient nitrogen removal after a sharp temperature decrease compared to 14 days SRT.

ACS Style

Antonina Kruglova; Jenni Kesulahti; Khoi Minh Le; Alejandro Gonzalez-Martinez; Anna Mikola; Riku Vahala. Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems. Water 2020, 12, 2450 .

AMA Style

Antonina Kruglova, Jenni Kesulahti, Khoi Minh Le, Alejandro Gonzalez-Martinez, Anna Mikola, Riku Vahala. Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems. Water. 2020; 12 (9):2450.

Chicago/Turabian Style

Antonina Kruglova; Jenni Kesulahti; Khoi Minh Le; Alejandro Gonzalez-Martinez; Anna Mikola; Riku Vahala. 2020. "Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems." Water 12, no. 9: 2450.

Journal article
Published: 18 August 2020 in Water
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In wastewater treatment plants, most microbial characterization has focused on bacterial, archaeal, and fungal populations. Due to the difficult isolation, quantification, and identification of viruses, only a limited number of virome studies associated with wastewater treatment plants have been carried out. However, the virus populations play an important role in the microbial dynamics in wastewater treatment systems and the biosafety of effluents. In this work, the viral members present in influent wastewater, mixed liquor (aerobic bioreactor), excess sludge, and effluent water of a conventional activated sludge system for the treatment of urban wastewater were identified. Viral members were observed by transmission electron microscopy and studied through next-generation sequencing studies. The results showed the dominance of bacteriophages in the viral community in all samples, with the dominant viral phylotype classified as Escherichia coli O157 typing phage 7. Moreover, different human viruses, such as Cynomolgus cytomegalovirus and Gammaherpesvirus, were also detected.

ACS Style

Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water 2020, 12, 2316 .

AMA Style

Cristina García-Fontana, Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Alejandro Gonzalez-Martinez, Maria Vela-Cano, Jesus Gonzalez-Lopez. Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain. Water. 2020; 12 (8):2316.

Chicago/Turabian Style

Cristina García-Fontana; Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Alejandro Gonzalez-Martinez; Maria Vela-Cano; Jesus Gonzalez-Lopez. 2020. "Profile of the Spatial Distribution Patterns of the Human and Bacteriophage Virome in a Wastewater Treatment Plant Located in the South of Spain." Water 12, no. 8: 2316.

Journal article
Published: 20 November 2019 in International Biodeterioration & Biodegradation
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The communities of Bacteria, Archaea and Fungi in a full-scale moving bed biofilm reactor in Rukatunturi (Ruka, Finland, in the Polar Arctic Circle) were analyzed in order to observe their ecological role in the operation of this system at low temperature. The bioreactor achieved efficient removal performances organic matter (>96%) and for ammonium (>95%), however a modest removal for total nitrogen (>63–75%). Bacteria dominant phylotypes changed in relation to the different functions: Trichococcus and Polaromonas for denitrification, Trichococcus and Simplicispira for organic matter removal, and Devosia and Thermomonas for nitrification. Bacteria predicted metagenome related to nitrification confirmed ammonium oxidation in nitrification chamber, and reduction of nitrate and nitrite in the denitrification and organic matter removal chambers. Dynamics of Fungi, dominated by Trichosporonaceae and Ascomycota clones, were linked to aeration conditions but not to substrate concentrations. Archaea communities, dominated by Methanobrevibacter genus and Thermoplasmatales-related clones, were not affected by aeration or substrate concentrations. The results obtained offer a valuable insight into the ecological role of microbial communities in full-scale moving bed biofilm reactor operating under extremely low temperatures.

ACS Style

Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Anna Mikola; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. Analysis of microbial communities involved in organic matter and nitrogen removal in a full-scale moving bed biofilm reactor located near the Polar Arctic Circle. International Biodeterioration & Biodegradation 2019, 146, 104830 .

AMA Style

Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Miguel Hurtado-Martinez, Anna Mikola, Jesus Gonzalez-Lopez, Riku Vahala, Alejandro Gonzalez-Martinez. Analysis of microbial communities involved in organic matter and nitrogen removal in a full-scale moving bed biofilm reactor located near the Polar Arctic Circle. International Biodeterioration & Biodegradation. 2019; 146 ():104830.

Chicago/Turabian Style

Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Anna Mikola; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. 2019. "Analysis of microbial communities involved in organic matter and nitrogen removal in a full-scale moving bed biofilm reactor located near the Polar Arctic Circle." International Biodeterioration & Biodegradation 146, no. : 104830.

Process systems engineering
Published: 01 October 2019 in AIChE Journal
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In a pilot plant under the stable‐stage of the two‐stage anaerobic digester, the dynamic of the archaeal, bacterial and eukaryal community structure was studied as well as their link to performance and biogas production. An effective synergic action between microorganisms was reached since biogas production and particularly methane (CH4) generation, were more than two‐times higher in the methanogenic respect to the acidogenic bioreactor. The α‐ and β‐ diversity analyses showed that dominant and rare operational taxonomic units of prokaryotic and eukaryotic microorganisms were critical in developing a functional successful anaerobic digestion process. A canonical correspondence analysis showed that acid/alkalinity ratio, volatile fatty acid and pH parameters mainly affected prokaryotic and eukaryotic phylotypes and a clear positive relationship was found between several phylotypes, such as Methanobacteriaceae, Unknown Thaumarchaeota, Intestinibacter, Coprothermobacter and Magnoliophyta, with biogas and CH4 production and performance parameters. This article is protected by copyright. All rights reserved.

ACS Style

María Jesús García‐Ruíz; Antonio Castellano‐Hinojosa; Caterina Armato; Alejandro González‐Martínez; Jesús González‐López; Francisco Osorio. Biogas production and microbial community structure in a stable‐stage of a two‐stage anaerobic digester. AIChE Journal 2019, 66, 1 .

AMA Style

María Jesús García‐Ruíz, Antonio Castellano‐Hinojosa, Caterina Armato, Alejandro González‐Martínez, Jesús González‐López, Francisco Osorio. Biogas production and microbial community structure in a stable‐stage of a two‐stage anaerobic digester. AIChE Journal. 2019; 66 (2):1.

Chicago/Turabian Style

María Jesús García‐Ruíz; Antonio Castellano‐Hinojosa; Caterina Armato; Alejandro González‐Martínez; Jesús González‐López; Francisco Osorio. 2019. "Biogas production and microbial community structure in a stable‐stage of a two‐stage anaerobic digester." AIChE Journal 66, no. 2: 1.

Journal article
Published: 24 May 2019 in Chemosphere
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The abundances of total and metabolically active populations of Candidatus Microthrix and Fungi were evaluated by quantitative PCR (qPCR) and retrotranscribed qPCR of ribosomal molecular markers in three different full-scale wastewater treatment plants (WWTPs), in absence of bulking/foaming episodes. Significant differences of the abundance of rDNAs and rRNAs of Candidatus Microthrix and Fungi were observed among the three WWTPs. The average relative abundances of 16S rDNA copies of Candidatus Microthrix to those of Bacteria ranged 3.4–8.9%. Biota-environment analysis (BIO-ENV) demonstrated that the number of copies of both 16S rDNA and rRNA of Candidatus Microthrix increased at longer hydraulic and solids' retention times and with higher nitrate concentrations in the activated sludge. The abundance of Candidatus Microthrix correlated strongly and positively with the removal efficiencies of organic matter and total nitrogen in the tested WWTPs, highlighting the role of these particular microbial group in the performance of these engineered systems.

ACS Style

P. Maza-Márquez; Antonio Castellano-Hinojosa; Alejandro Gonzalez-Martinez; B. Juárez-Jiménez; J. González-López; B. Rodelas. Abundance of total and metabolically active Candidatus Microthrix and fungal populations in three full-scale wastewater treatment plants. Chemosphere 2019, 232, 26 -34.

AMA Style

P. Maza-Márquez, Antonio Castellano-Hinojosa, Alejandro Gonzalez-Martinez, B. Juárez-Jiménez, J. González-López, B. Rodelas. Abundance of total and metabolically active Candidatus Microthrix and fungal populations in three full-scale wastewater treatment plants. Chemosphere. 2019; 232 ():26-34.

Chicago/Turabian Style

P. Maza-Márquez; Antonio Castellano-Hinojosa; Alejandro Gonzalez-Martinez; B. Juárez-Jiménez; J. González-López; B. Rodelas. 2019. "Abundance of total and metabolically active Candidatus Microthrix and fungal populations in three full-scale wastewater treatment plants." Chemosphere 232, no. : 26-34.

Journal article
Published: 09 May 2019 in Journal of Hazardous Materials
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The present work aims to use aerobic granular sludge technology for the treatment of wastewater containing high organic matter loads and a mixture of phenolic compounds normally present in olive washing water. The physicochemical performance of five bioreactors treating different concentrations of mixture of phenolic acid was monitored to observe the response of the systems. The bioreactors that operated at 50, 100 and 300 mg L−1 did not show relevant changes in terms of performance and granules properties, showing high ratio of phenolic compound removal ratio. However, the bioreactors operated with high phenolic compound concentrations showed low rates of organic matter, nitrogen and phenolic acid removal. In the same way, high concentrations of phenolic compounds determined the disintegration of the granular biomass. Next-generation sequencing studies showed a stable community structure in the bioreactors operating with 50, 100 and 300 mg L−1 of phenolic acids, with the genera Lampropedia and Arenimonas, family Xanthobacteraceae and Fungi Pezizomycotina as the dominant phylotypes. Conversely, the reactors operated at 500 and 600 mg L−1 of phenolic substances promoted the proliferation of Oligohymenophorea ciliates. Thus, this study suggests that aerobic granular sludge technology could be useful for the treatment of wastewaters such as olive washing water.

ACS Style

Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Ines Manuel de Castro; Belén Juarez-Jimenez; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez. Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations. Journal of Hazardous Materials 2019, 376, 58 -67.

AMA Style

Barbara Muñoz-Palazon, Alejandro Rodriguez-Sanchez, Miguel Hurtado-Martinez, Ines Manuel de Castro, Belén Juarez-Jimenez, Alejandro Gonzalez-Martinez, Jesus Gonzalez-Lopez. Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations. Journal of Hazardous Materials. 2019; 376 ():58-67.

Chicago/Turabian Style

Barbara Muñoz-Palazon; Alejandro Rodriguez-Sanchez; Miguel Hurtado-Martinez; Ines Manuel de Castro; Belén Juarez-Jimenez; Alejandro Gonzalez-Martinez; Jesus Gonzalez-Lopez. 2019. "Performance and microbial community structure of an aerobic granular sludge system at different phenolic acid concentrations." Journal of Hazardous Materials 376, no. : 58-67.

Journal article
Published: 05 March 2019 in Chemosphere
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A lab-scale partial nitritation SBR was operated at 11 °C for 300 days used for the treatment of high-ammonium wastewater, which was inoculated with activated sludge from Rovaniemi WWTP (located in Polar Arctic Circle) in order to evaluate the influence the temperature on the performance, stability and dynamics of its microbial community. The partial nitritation achieved steady-state long-term operation and granulation process was not affected despite the low temperature and high ammonia concentration. The steady conditions were reached after 60 days of operation where the granular biomass was fully-formed and the 50%–50% of ammonium-nitrite effluent was successful achieved. Inoculation with cold adapted inoculum showed to yield bigger, denser granules with faster start-up without necessity of low temperature adaptation period. Next-generation sequences techniques showed that Trichosporonaceae and Xanthomonadaceae were the dominant OTUs in the mature granules. Our study could be useful in the implementation of full-scale partial nitritation reactors in cold regions such as Nordic countries for treating wastewater with high concentration of ammonium.

ACS Style

Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Paula Maza-Marquez; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature. Chemosphere 2019, 225, 73 -82.

AMA Style

Alejandro Rodriguez-Sanchez, Barbara Muñoz-Palazon, Miguel Hurtado-Martinez, Paula Maza-Marquez, Jesus Gonzalez-Lopez, Riku Vahala, Alejandro Gonzalez-Martinez. Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature. Chemosphere. 2019; 225 ():73-82.

Chicago/Turabian Style

Alejandro Rodriguez-Sanchez; Barbara Muñoz-Palazon; Miguel Hurtado-Martinez; Paula Maza-Marquez; Jesus Gonzalez-Lopez; Riku Vahala; Alejandro Gonzalez-Martinez. 2019. "Microbial ecology dynamics of a partial nitritation bioreactor with Polar Arctic Circle activated sludge operating at low temperature." Chemosphere 225, no. : 73-82.

Chapter
Published: 09 December 2018 in Nanomaterial Biointeractions at the Cellular, Organismal and System Levels
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Phenolic compounds (PCs) of either natural or anthropogenic origin are pollutants often occurring in industrial, agricultural, or domestic wastewaters, which are toxic for living organisms even when present at concentrations lower than 1 mg/L in aquatic media. Different physicochemical or biological strategies have been designed, tested, and applied for the removal of PCs from wastewaters; biological approaches are most often preferred for their efficiency at a lower cost. The ability to remove PCs of various types of microorganisms (bacteria, archaea, fungi, and microalgae), either isolated or in consortia, has been widely described in the literature. Photobioreactors (PBRs) are a reliable and efficient technology to treat complex wastewater effluents, based on the mutualistic relationships among microalgae and bacteria. Microalgae-bacteria consortia provide a variety of advantages for wastewater treatment, since photoautotrophic microorganisms deliver O2 to heterotrophic bacteria while fixing the CO2 generated by the mineralization of organic matter, thus reducing aeration cost and greenhouse gas emissions. Since microbial communities determine the success of the biological strategies for the removal of pollutants in PBRs, different biotic and abiotic factors influencing their diversity and functions are critical and must be considered. In this chapter, we focused on the current knowledge regarding the potential of microalgae-bacteria consortia for the effective treatment of PCs in wastewaters using PBR systems.

ACS Style

Paula Maza-Márquez; Alejandro González-Martínez; Belén Juárez-Jiménez; Belén Rodelas; Jesús González-López. Microalgae-Bacteria Consortia for the Removal of Phenolic Compounds from Industrial Wastewaters. Nanomaterial Biointeractions at the Cellular, Organismal and System Levels 2018, 135 -184.

AMA Style

Paula Maza-Márquez, Alejandro González-Martínez, Belén Juárez-Jiménez, Belén Rodelas, Jesús González-López. Microalgae-Bacteria Consortia for the Removal of Phenolic Compounds from Industrial Wastewaters. Nanomaterial Biointeractions at the Cellular, Organismal and System Levels. 2018; ():135-184.

Chicago/Turabian Style

Paula Maza-Márquez; Alejandro González-Martínez; Belén Juárez-Jiménez; Belén Rodelas; Jesús González-López. 2018. "Microalgae-Bacteria Consortia for the Removal of Phenolic Compounds from Industrial Wastewaters." Nanomaterial Biointeractions at the Cellular, Organismal and System Levels , no. : 135-184.

Research article
Published: 09 October 2018 in Biotechnology Progress
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The effect of antibiotics sulfadiazine and trimethoprim on activated sludge operated at 8 °C was investigated. Performance and microbial communities of sequencing batch reactors (SBR) and Membrane Bioreactors (MBR) were compared before and after the exposure of antibiotics to the synthetic wastewater. The results revealed irreversible negative effect of these antibiotics in environmentally relevant concentrations on nitrifying microbial community of SBR activated sludge. In opposite, MBR sludge demonstrated fast adaptation and more stable performance during the antibiotics exposure. Dynamics of microbial community was greatly affected by presence of antibiotics. Bacteria from classes Betaproteobacteria and Bacteroidetes demonstrated the potential to develop antibiotic resistance in both wastewater treatment systems whilst Actinobacteria disappeared from all of the reactors after 60 days of antibiotics exposure. Altogether, results showed that operational parameters such as sludge retention time (SRT) and reactor configuration had great effect on microbial community composition of activated sludge and its vulnerability to antibiotics. Operation at long SRT allowed archaea, including ammonium oxidizing species (AOA) such as Nitrososphaera viennensis to grow in MBRs. AOA could have an important role in stable nitrification performance of MBR activated sludge due to tolerance of archaea to antibiotics. This article is protected by copyright. All rights reserved.

ACS Style

Antonina Kruglova; Anna Mikola; Alejandro Gonzalez-Martinez; Riku Vahala. Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C. Biotechnology Progress 2018, 35, e2708 .

AMA Style

Antonina Kruglova, Anna Mikola, Alejandro Gonzalez-Martinez, Riku Vahala. Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C. Biotechnology Progress. 2018; 35 (1):e2708.

Chicago/Turabian Style

Antonina Kruglova; Anna Mikola; Alejandro Gonzalez-Martinez; Riku Vahala. 2018. "Effect of sulfadiazine and trimethoprim on activated sludge performance and microbial community dynamics in laboratory-scale membrane bioreactors and sequencing batch reactors at 8°C." Biotechnology Progress 35, no. 1: e2708.

Journal article
Published: 01 October 2018 in Journal of Environmental Management
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The abundance of fungi in a full-scale membrane bioreactor (MBR) treating urban wastewater and experiencing seasonal foaming was assessed by quantitative PCR (qPCR), comparing three different sets of widely used universal fungal primers targeting the gene encoding the small ribosomal subunit RNA, 18S-rDNA, (primers NS1-Fung and FungiQuant) or the internal transcribed spacer ITS2 (primers ITS3-ITS4). Fungi were a numerically important fraction of the MBR microbiota (≥10 18S-rDNA copies/L activated sludge), and occurred both in the aerated and anoxic bioreactors. The numbers of copies of fungal markers/L activated sludge calculated using the NS1-Fung or ITS3-ITS4 primer sets were up to 2 orders of magnitude higher than the quantifications based on the FungiQuant primers. Fungal 18S-rDNA counts derived from the FungiQuant primers decreased significantly during cold seasons, concurring with foaming episodes in the MBR. Redundancy analysis corroborated that temperature was the main factor driving fungi abundance, which was also favored by longer solid retention time (SRT), lower chemical oxygen demand/biochemical oxygen demand at 5 days (COD/BOD) of influent water, and lower biomass accumulation in the MBR.

ACS Style

P. Maza-Márquez; R. Vílchez-Vargas; Alejandro Gonzalez-Martinez; J. González-López; B. Rodelas. Assessing the abundance of fungal populations in a full-scale membrane bioreactor (MBR) treating urban wastewater by using quantitative PCR (qPCR). Journal of Environmental Management 2018, 223, 1 -8.

AMA Style

P. Maza-Márquez, R. Vílchez-Vargas, Alejandro Gonzalez-Martinez, J. González-López, B. Rodelas. Assessing the abundance of fungal populations in a full-scale membrane bioreactor (MBR) treating urban wastewater by using quantitative PCR (qPCR). Journal of Environmental Management. 2018; 223 ():1-8.

Chicago/Turabian Style

P. Maza-Márquez; R. Vílchez-Vargas; Alejandro Gonzalez-Martinez; J. González-López; B. Rodelas. 2018. "Assessing the abundance of fungal populations in a full-scale membrane bioreactor (MBR) treating urban wastewater by using quantitative PCR (qPCR)." Journal of Environmental Management 223, no. : 1-8.

Journal article
Published: 04 September 2018 in Bioresource Technology
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Two microbial fuel cells were inoculated with activated sludge from Finland and operated under moderate (25 °C) and low (8 °C) temperatures. Operation under real urban wastewater showed similarities in chemical oxygen demand removal and voltage generated, although moderate temperature supported higher ammonium oxidation. Fungi disappeared in the microbial fuel cell operated at temperature of 25 °C. Archaea domain was dominated by methanogenic archaea at both temperature scenarios. Important differences were observed in bacterial communities between both temperatures, however generating similar voltage. The results supported that the implementation of microbial fuel cells in Nordic countries operating under real conditions could be successful, as well as suggested the flexibility of cold-adapted inoculum for starting-up microbial fuel cells, regardless of the operating temperature of the system, obtaining higher COD removal and voltage generation performances at low temperature than at moderate temperature.

ACS Style

Alejandro Gonzalez-Martínez; Su Chengyuan; Alejandro Rodriguez-Sanchez; Clementina Pozo; Jesus Gonzalez-Lopez; Riku Vahala. Application of microbial fuel cell technology for wastewater treatment and electricity generation under Nordic countries climate conditions: Study of performance and microbial communities. Bioresource Technology 2018, 270, 1 -10.

AMA Style

Alejandro Gonzalez-Martínez, Su Chengyuan, Alejandro Rodriguez-Sanchez, Clementina Pozo, Jesus Gonzalez-Lopez, Riku Vahala. Application of microbial fuel cell technology for wastewater treatment and electricity generation under Nordic countries climate conditions: Study of performance and microbial communities. Bioresource Technology. 2018; 270 ():1-10.

Chicago/Turabian Style

Alejandro Gonzalez-Martínez; Su Chengyuan; Alejandro Rodriguez-Sanchez; Clementina Pozo; Jesus Gonzalez-Lopez; Riku Vahala. 2018. "Application of microbial fuel cell technology for wastewater treatment and electricity generation under Nordic countries climate conditions: Study of performance and microbial communities." Bioresource Technology 270, no. : 1-10.

Journal article
Published: 25 August 2018 in Water
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Two pilot-scale hybrid moving bed biofilm reactor-membrane bioreactors were operated in parallel for the treatment of salinity-amended urban wastewater under 6 hours of hydraulic retention time and 2500 mg L−1 total solids concentration. Two salinity conditions were tested: the constant salinity of 6.5 mS cm−1 electric conductivity (3.6 g L−1 NaCl) and the tidal-like variable salinity with maximum 6.5 mS cm−1 electric conductivity. An investigation was developed on the biofouling produced on the ultrafiltration membrane surface evaluating its bacterial community structure and its potential function in the fouling processes. The results showed that biofouling was clearly affected by salinity scenarios in terms of α-diversity and β-diversity and bacterial community structure, which confirms lower bacterial diversity under variable salinity conditions with Rhodanobacter and Dyella as dominant phylotypes. Microorganisms identified as bio-mineral formers belonged to genera Bacillus, Citrobacter, and Brevibacterium. These findings will be of help for the prevention and control of biofouling in saline wastewater treatment systems.

ACS Style

Alejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water 2018, 10, 1133 .

AMA Style

Alejandro Rodriguez-Sanchez, Juan Carlos Leyva-Diaz, Barbara Muñoz-Palazon, Maria Angustias Rivadeneyra, Miguel Hurtado-Martinez, Daniel Martin-Ramos, Alejandro Gonzalez-Martinez, Jose Manuel Poyatos, Jesus Gonzalez-Lopez. Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration. Water. 2018; 10 (9):1133.

Chicago/Turabian Style

Alejandro Rodriguez-Sanchez; Juan Carlos Leyva-Diaz; Barbara Muñoz-Palazon; Maria Angustias Rivadeneyra; Miguel Hurtado-Martinez; Daniel Martin-Ramos; Alejandro Gonzalez-Martinez; Jose Manuel Poyatos; Jesus Gonzalez-Lopez. 2018. "Biofouling Formation and Bacterial Community Structure in Hybrid Moving Bed Biofilm Reactor-Membrane Bioreactors: Influence of Salinity Concentration." Water 10, no. 9: 1133.

Journal article
Published: 01 August 2018 in Chemosphere
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Three aerobic granular sequencing batch reactors were inoculated using different inocula from Finland, Spain and a mix of both in order to investigate the effect over the degradation performance and the microbial community structure. The Finnish inoculum achieved a faster granulation and a higher depollution performance within the first two month of operation. However, after 90 days of operation, similar physico-chemical values were observed. On the other hand, the Real-time PCR showed that Archaea diminished from inoculum to granular biomass, while Bacteria and Fungi numbers remained stable. All granular biomass massive parallel sequencing studies were similar regardless of the inocula from which they formed, as confirmed by singular value decomposition principal coordinates analysis, expected effect size of OTUs, and β-diversity analyses. Thermoproteaceae, Meganema and a Trischosporonaceae members were the dominant phylotypes for the three domains studied. The analysis of oligotype distribution demonstrated that a fungal oligotype was ubiquitous. The dominant OTUs of Bacteria were correlated with bioreactors performance. The results obtained determined that the microbial community structure of aerobic granular sludge was similar regardless of their inocula, showing that the granulation of biomass is related to several phylotypes. This will be of future importance for the implementation of aerobic granular sludge to full-scale systems.

ACS Style

Barbara Muñoz-Palazon; Chiara Pesciaroli; Alejandro Rodriguez-Sanchez; Jesús Gonzalez-Lopez; Alejandro Gonzalez-Martinez. Pollutants degradation performance and microbial community structure of aerobic granular sludge systems using inoculums adapted at mild and low temperature. Chemosphere 2018, 204, 431 -441.

AMA Style

Barbara Muñoz-Palazon, Chiara Pesciaroli, Alejandro Rodriguez-Sanchez, Jesús Gonzalez-Lopez, Alejandro Gonzalez-Martinez. Pollutants degradation performance and microbial community structure of aerobic granular sludge systems using inoculums adapted at mild and low temperature. Chemosphere. 2018; 204 ():431-441.

Chicago/Turabian Style

Barbara Muñoz-Palazon; Chiara Pesciaroli; Alejandro Rodriguez-Sanchez; Jesús Gonzalez-Lopez; Alejandro Gonzalez-Martinez. 2018. "Pollutants degradation performance and microbial community structure of aerobic granular sludge systems using inoculums adapted at mild and low temperature." Chemosphere 204, no. : 431-441.

Journal article
Published: 01 May 2018 in Bioresource Technology
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The aim of this work was to study the performance and microbial community structure of a polar Arctic Circle aerobic granular sludge (AGS) system operating at low temperature. Thus, an AGS bioreactor was operated at 7, 5 and 3 °C of temperature using a cold-adapted sludge from Lapland. At 5 °C, it yielded acceptable conversion rates, in terms of nitrogen, phosphorous, and organic matter. However, under 3 °C a negligible nitrogen and phosphorous removal performance was observed. Below 5 °C, scanning electron microscopy studies showed a wispy, non-dense and irregular granular structure with a strong outgrowth of filamentous. Moreover, Illumina next-generation sequencing showed a heterogeneous microbial population where SM1K20 (Archaea), Trichosporon domesticum (Fungus), and Zooglea, Arcobacter and Acinetobacter (Bacteria) were the dominant phylotypes. Our study suggests that AGS technologies inoculated with North Pole sludge could be operated, in cold regions for a period longer than 3 months (winter season) under 5 °C of water temperature.

ACS Style

Alejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Paula Maza-Márquez; Alejandro Rodriguez-Sanchez; Jesus Gonzalez-Lopez; Riku Vahala. Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature. Bioresource Technology 2018, 256, 22 -29.

AMA Style

Alejandro Gonzalez-Martinez, Barbara Muñoz Palazon, Paula Maza-Márquez, Alejandro Rodriguez-Sanchez, Jesus Gonzalez-Lopez, Riku Vahala. Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature. Bioresource Technology. 2018; 256 ():22-29.

Chicago/Turabian Style

Alejandro Gonzalez-Martinez; Barbara Muñoz Palazon; Paula Maza-Márquez; Alejandro Rodriguez-Sanchez; Jesus Gonzalez-Lopez; Riku Vahala. 2018. "Performance and microbial community structure of a polar Arctic Circle aerobic granular sludge system operating at low temperature." Bioresource Technology 256, no. : 22-29.

Mini review
Published: 30 April 2018 in Applied Microbiology and Biotechnology
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Waste treatment and the simultaneous production of energy have gained great interest in the world. In the last decades, scientific efforts have focused largely on improving and developing sustainable bioprocess solutions for energy recovery from challenging waste. Anaerobic digestion (AD) has been developed as a low-cost organic waste treatment technology with a simple setup and relatively limited investment and operating costs. Different technologies such as one-stage and two-stage AD have been developed. The viability and performance of these technologies have been extensively reported, showing the supremacy of two-stage AD in terms of overall energy recovery from biomass under different substrates, temperatures, and pH conditions. However, a comprehensive review of the advantages and disadvantages of these technologies is still lacking. Since microbial ecology is critical to developing successful AD, many studies have shown the structure and dynamics of archaeal and bacterial communities in this type of system. However, the role of Eukarya groups remains largely unknown to date. In this review, we provide a comprehensive review of the role, abundance, dynamics, and structure of archaeal, bacterial, and eukaryal communities during the AD process. The information provided could help researchers to select the adequate operational parameters to obtain the best performance and biogas production results.

ACS Style

Antonio Castellano-Hinojosa; Caterina Armato; Clementina Pozo; Alejandro Gonzalez-Martinez; Jesús González-López. New concepts in anaerobic digestion processes: recent advances and biological aspects. Applied Microbiology and Biotechnology 2018, 102, 5065 -5076.

AMA Style

Antonio Castellano-Hinojosa, Caterina Armato, Clementina Pozo, Alejandro Gonzalez-Martinez, Jesús González-López. New concepts in anaerobic digestion processes: recent advances and biological aspects. Applied Microbiology and Biotechnology. 2018; 102 (12):5065-5076.

Chicago/Turabian Style

Antonio Castellano-Hinojosa; Caterina Armato; Clementina Pozo; Alejandro Gonzalez-Martinez; Jesús González-López. 2018. "New concepts in anaerobic digestion processes: recent advances and biological aspects." Applied Microbiology and Biotechnology 102, no. 12: 5065-5076.