The Ames assay is the standard assay for identifying DNA-reactive genotoxic substances. Multiple formats are available and the correct choice of an assay protocol is essential for achieving optimal performance, including fit for purpose detection limits and required screening capacity. In the present study, a comparison of those parameters between two commonly used formats, the standard pre-incubation Ames test and the liquid-based Ames MPF™, was performed. For that purpose, twenty-one substances with various modes of action were chosen and tested for their lowest effect concentrations (LEC) with both tests. In addition, two sources of rat liver homogenate S9 fraction, Aroclor 1254-induced and phenobarbital/β-naphthoflavone induced, were compared in the Ames MPF™. Overall, the standard pre-incubation Ames and the Ames MPF™ assay showed high concordance (>90%) for mutagenic vs. non-mutagenic compound classification. The LEC values of the Ames MPF™ format were lower for 17 of the 21 of the selected test substances. The S9 source had no impact on the test results. This leads to the conclusion that the liquid-based Ames MPF™ assay format provides screening advantages when low concentrations are relevant, such as in the testing of complex mixtures.

One of the EU’s ambitious goals is to ensure all plastic packaging is reusable or recyclable by 2030. However, achieving higher recycling rates, particularly in flexible multilayer food packaging, is challenging due to the combination of diverse materials. This highlights the importance of designing for recyclability and exploring alternative barrier solutions. In this study, several types of coatings, such as ORMOCER^® (Fraunhofer-ISC, Würzburg, Germany) Michem^® Flex B3513 (Michelman International Belgium SRL, Aubange, Belgium), and PVOH (Kuraray Europe GmbH, Frankfurt am Main, Germany), are applied on different polypropylene substrates, including cast PP (CPP70), SiOx, and AlOx-coated PP films. The effect of double coating and optimized curing conditions of ORMOCER^® on the oxygen permeability of CPP70 was also investigated. The results showed significant improvements in the barrier properties of PP/SiOx and OPP/AlOx films, and OTR values less than 0.1 cm³/m²·d·bar were achieved. It was also found that ORMOCER^® and Michem^® Flex B3513 could enhance the oxygen barrier property of CPP70 and the OTR value reduced by a factor of 88 and 551, respectively.

Food loss and waste have been identified as significant contributors to existing environmental challenges. Previous studies have extensively quantified losses and waste throughout the value chain. However, there is a lack of knowledge regarding the influence of packaging design on food residue quantities. This study analyses the technical emptiability of dairy product packaging, building upon previously described methods and proposing new methods for a standardized analysis. The results demonstrate significant variations in residue amounts depending on product type, fat content, viscosity, packaging type and design, as well as consumer handling. The findings indicate that residues of high-viscosity products, such as yoghurt drinks and buttermilk, can accumulate to a level exceeding 4% of the total filling weight in the packaging; meanwhile, the residues of low-viscosity products, such as milk, collectively represent less than 1% of the total filling weight. Consumer handling instructions on packaging significantly reduce residues, as shown by the instruction to shake before opening, which notably decreases the residues of high-viscosity products. Future legislation to minimize food waste and reduce the environmental impact of packaging will necessitate that the packaging industry produces easy-to-empty packaging. This will improve sorting, recycling, recyclate quality, and environmental impact, consequently enhancing the sustainability of dairy packaging.

The need for increased recycling of plastic packaging waste (PPW) is apparent from a legal and waste management perspective and, therefore, further waste streams need to be investigated in detail in terms of their recycling potential. Polyethylene terephthalate (PET) PW is already closed-loop recyclable (bottle-to-bottle recycling); however, other rigid PET PW is mainly thermally recovered. Explicit quantitative and qualitative data on rigid PET packaging waste are limited. Therefore, this study investigates the composition and packaging characteristics of rigid PET packaging waste contained in separate waste collection as well as in the mixed PET sorting stream in Austria by conducting a manual sorting analysis. Furthermore, the waste volume is projected, and the recycling potential is extrapolated according to new European recycling rate reporting formats. The results show that approximately 11% of separate collection represents rigid PET packaging waste. Most PW derives from food packaging and is transparent. Contained residues with more than 1% of the total packaging weight might negatively impact the sortability. The applicable net quantity indicator (ALR) amounts to 0.888 at the stage of sorting. The volume of rigid PET PW is extrapolated to 26–36 kt in 2020 with a high-quality recycling rate of 25%, which contributes 2.6% to the Austrian PPW recycling target of 50%.

The European Union aims for climate neutrality by 2050 and has proposed the Packaging and Packing Waste Regulation (PPWR) to promote a circular economy, focusing on reducing packaging waste. In this context, a comprehensive sustainability assessment for liquid dairy product packaging, including beverage cartons, bottles and to-go cups, in the DACH region (Germany, Austria and Switzerland) was conducted. The aim was to consider various ecological aspects of environmental impacts and circularity. As the aspect of recyclability is a core aspect in the PPWR, the calculation was of central interest in this project. Here, major differences in the waste management infrastructure between countries could be identified. The majority of assessed packaging falls below the PPWR’s 70% recyclability requirement, with Switzerland showing even lower recyclability due to poor packaging collection and recycling infrastructure. Significant discrepancies in packaging efficiency exist, indicating unnecessary resource consumption, especially in the case of to-go cups. Additionally, the carbon footprint of packaging materials can vary up to ten times within certain product categories, negatively impacting the environment. Good results were identified for the use of certified renewable resources. Overall, the results of the assessment demonstrate several areas for improvement in light of forthcoming regulatory requirements, which must be met in Germany and Austria.

The adoption of the PPWR by the Council of the European Union (EU/2025/40) in 2025 intensifies the regulatory pressure on packaging manufacturers and food producers. This necessitates their adaptation of packaging to the new standards and selection of materials by various sustainability criteria and minimum standards. The legal text places particular emphasis on recyclability and the carbon footprint. The dairy industry holds significant economic importance in the DACH region (Germany, Austria, and Switzerland); this study therefore analyzed and compared the recyclability and carbon footprint of selected product categories and diverse packaging options for butter, yogurt, spreads, curd cheese, and cottage cheese. This study found large differences within the product categories, and also between the assessed countries, due to differing waste management systems (collection, sorting, and recycling) and waste streams. A key finding is the substantial discrepancy in glass packaging, which significantly exceeds the emission values of other packaging systems. In terms of recyclability, glass packaging performs well due to the effectiveness of the recycling systems regarding the future effective PPWR. However, significant variations in recyclability were observed between Germany and Austria for tray and cup materials made of PP and PS. Notably, there is a preference for packaging systems without a cardboard sleeve over those with one. This study provides critical insights into the environmental performance of packaging materials in the dairy sector. It highlights the challenges posed by regulatory changes and the urgent need for region-specific strategies. By identifying key areas for improvement in packaging design and waste management, this work lays the foundation for achieving compliance with the PPWR and advancing the transition toward a circular economy in the DACH region.