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Prof. Dr. Silvia Apprich
Department of Packaging and Resource Management, University of Applied Sciences, Vienna 1030, Austria

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0 Food Microbiology
0 Food Packaging
0 Food Safety
0 Food Technology
0 Shelf Life

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Journal article
Published: 29 June 2021 in Toxics
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The Ames assay is the standard assay for identifying DNA-reactive genotoxic substances. Multiple formats are available and the correct choice of an assay protocol is essential for achieving optimal performance, including fit for purpose detection limits and required screening capacity. In the present study, a comparison of those parameters between two commonly used formats, the standard pre-incubation Ames test and the liquid-based Ames MPF™, was performed. For that purpose, twenty-one substances with various modes of action were chosen and tested for their lowest effect concentrations (LEC) with both tests. In addition, two sources of rat liver homogenate S9 fraction, Aroclor 1254-induced and phenobarbital/β-naphthoflavone induced, were compared in the Ames MPF™. Overall, the standard pre-incubation Ames and the Ames MPF™ assay showed high concordance (>90%) for mutagenic vs. non-mutagenic compound classification. The LEC values of the Ames MPF™ format were lower for 17 of the 21 of the selected test substances. The S9 source had no impact on the test results. This leads to the conclusion that the liquid-based Ames MPF™ assay format provides screening advantages when low concentrations are relevant, such as in the testing of complex mixtures.

ACS Style

Bernhard Rainer; Elisabeth Pinter; Lukas Prielinger; Chiara Coppola; Maricel Marin-Kuan; Benoit Schilter; Silvia Apprich; Manfred Tacker. Direct Comparison of the Lowest Effect Concentrations of Mutagenic Reference Substances in Two Ames Test Formats. Toxics 2021, 9, 152 .

AMA Style

Bernhard Rainer, Elisabeth Pinter, Lukas Prielinger, Chiara Coppola, Maricel Marin-Kuan, Benoit Schilter, Silvia Apprich, Manfred Tacker. Direct Comparison of the Lowest Effect Concentrations of Mutagenic Reference Substances in Two Ames Test Formats. Toxics. 2021; 9 (7):152.

Chicago/Turabian Style

Bernhard Rainer; Elisabeth Pinter; Lukas Prielinger; Chiara Coppola; Maricel Marin-Kuan; Benoit Schilter; Silvia Apprich; Manfred Tacker. 2021. "Direct Comparison of the Lowest Effect Concentrations of Mutagenic Reference Substances in Two Ames Test Formats." Toxics 9, no. 7: 152.