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Squash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceará, Maranhão, Piauí, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be completely risk-free. Similarities in gene repertories between these viruses and viruses that affect animal species have been reported. The present study describes the fully sequenced genomes of SqMV found in human feces, collected in Tocantins, and analyzes the viral profile by metagenomics in the context of diarrhea symptomatology. The complete SqMV genome was obtained in 39 of 253 analyzed samples (15.5%); 97.4% of them belonged to children under 5 years old. There was no evidence that the observed symptoms were related to the presence of SqMV. Of the different virus species detected in these fecal samples, at least 4 (rotavirus, sapovirus, norovirus, parechovirus) are widely known to cause gastrointestinal symptoms. The presence of SqMV nucleic acid in fecal samples is likely due to recent dietary consumption and it is not evidence of viral replication in the human intestinal cells. Identifying the presence of SqMV in human feces and characterization of its genome is a relevant precursor to determining whether and how plant viruses interact with host cells or microorganisms in the human gastrointestinal tract.
Fabiola Villanova; Roberta Marcatti; Mayara Bertanhe; Vanessa Morais; Flavio Milagres; Rafael Brustulin; Emerson Araújo; Roozbeh Tahmasebi; Steven Witkin; Xutao Deng; Eric Delwart; Ester Sabino; Cassio Abreu-Junior; Élcio Leal; Antonio Costa. New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples. Microorganisms 2021, 9, 1349 .
AMA StyleFabiola Villanova, Roberta Marcatti, Mayara Bertanhe, Vanessa Morais, Flavio Milagres, Rafael Brustulin, Emerson Araújo, Roozbeh Tahmasebi, Steven Witkin, Xutao Deng, Eric Delwart, Ester Sabino, Cassio Abreu-Junior, Élcio Leal, Antonio Costa. New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples. Microorganisms. 2021; 9 (7):1349.
Chicago/Turabian StyleFabiola Villanova; Roberta Marcatti; Mayara Bertanhe; Vanessa Morais; Flavio Milagres; Rafael Brustulin; Emerson Araújo; Roozbeh Tahmasebi; Steven Witkin; Xutao Deng; Eric Delwart; Ester Sabino; Cassio Abreu-Junior; Élcio Leal; Antonio Costa. 2021. "New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples." Microorganisms 9, no. 7: 1349.
Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples (n = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapá. Twelve DENV type 1 (DENV-1) genomes were identified, which were phylogenetically related to the BR4 lineage of genotype V. Phylodynamics analysis suggested that DENV-1 BR-4 was introduced into Amapá around early 2010, possibly from other states in northern Brazil. We also found unique amino acids substitutions in the DENV-1 envelope and NS5 protein sequences in the Amapá isolates. Characterization of the DENV-1 BR-4 sequences highlights the potential of this new lineage to drive outbreaks of dengue in the Amazon region.
Geovani Ribeiro; Danielle Gill; Edcelha Ribeiro; Fred Monteiro; Vanessa Morais; Roberta Marcatti; Marlisson Rego; Emerson Araújo; Steven Witkin; Fabiola Villanova; Xutao Deng; Ester Sabino; Eric Delwart; Élcio Leal; Antonio da Costa. Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon. Viruses 2021, 13, 689 .
AMA StyleGeovani Ribeiro, Danielle Gill, Edcelha Ribeiro, Fred Monteiro, Vanessa Morais, Roberta Marcatti, Marlisson Rego, Emerson Araújo, Steven Witkin, Fabiola Villanova, Xutao Deng, Ester Sabino, Eric Delwart, Élcio Leal, Antonio da Costa. Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon. Viruses. 2021; 13 (4):689.
Chicago/Turabian StyleGeovani Ribeiro; Danielle Gill; Edcelha Ribeiro; Fred Monteiro; Vanessa Morais; Roberta Marcatti; Marlisson Rego; Emerson Araújo; Steven Witkin; Fabiola Villanova; Xutao Deng; Ester Sabino; Eric Delwart; Élcio Leal; Antonio da Costa. 2021. "Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon." Viruses 13, no. 4: 689.
Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.
Endrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses 2021, 13, 595 .
AMA StyleEndrya Do Socorro Fôro Ramos, Ulisses Rosa, Geovani De Oliveira Ribeiro, Fabiola Villanova, Flávio de Pádua Milagres, Rafael Brustulin, Vanessa Dos Santos Morais, Mayara Bertanhe, Roberta Marcatti, Emerson Araújo, Steven Witkin, Eric Delwart, Adriana Luchs, Antonio da Costa, Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses. 2021; 13 (4):595.
Chicago/Turabian StyleEndrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. 2021. "High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis." Viruses 13, no. 4: 595.
Bovine parainfluenza virus type 3 (BPIV3) is one of the most important known viral respiratory pathogens of both young and adult cattle. It is also named "heat stress in transport", causing morbidity and mass death. New variants of BPIV3 have been detected or isolated in China since 2008. Here, we isolate one BPIV3 strain (named BPIV3 BJ) in Madin-Darby bovine kidney (MDBK) cells from nasal samples collected in China. Phylogenetic analysis showed that our isolate is related to BPIV3 of the genotype A. The comparison of BPIV3-BJ and the reference Chinese isolate NM09 showed that these strains are highly divergent. We found many differences in the amino acid composition in the nucleocapsid (NP) protein among these genotype A strains. Since the NP protein has been implicated in immunization studies, our BPIV3 isolate will be useful for the development of immune assays and vaccine studies. The diversity of BPIV3 lineages that we found in China indicated ongoing evolution for immune escape. Our study highlights the importance of genetic surveillance for determining the effect of BPIV3 variability on pathogen evolution and population-scale immunity.
Élcio Leal; Cun Liu; Zhanzhong Zhao; Yong Deng; Fabiola Villanova; Lin Liang; Jinxiang Li; Shangjin Cui. Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China. Viruses 2019, 11, 489 .
AMA StyleÉlcio Leal, Cun Liu, Zhanzhong Zhao, Yong Deng, Fabiola Villanova, Lin Liang, Jinxiang Li, Shangjin Cui. Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China. Viruses. 2019; 11 (6):489.
Chicago/Turabian StyleÉlcio Leal; Cun Liu; Zhanzhong Zhao; Yong Deng; Fabiola Villanova; Lin Liang; Jinxiang Li; Shangjin Cui. 2019. "Isolation of a Divergent Strain of Bovine Parainfluenza Virus Type 3 (BPIV3) Infecting Cattle in China." Viruses 11, no. 6: 489.
The peptides Tx2-5 and Tx2-6, isolated from the whole venom of “armed-spider” Phoneutria nigriventer venom, are directly linked with the induction of persistent and painful erection in the penis of mammals. The erection induced by Tx2-6 has been associated with the activation of nitric oxide synthases. There is a scarcity of studies focusing on the outcome of Tx2-6 at the molecular level, by this reason we evaluated the gene profile activity of this toxin at the nitric oxide (NO) pathway. After microarray analyses on cavernous tissue of mice inoculated with Tx2-6 we found that only 10.4% (10/96) of these genes were differentially expressed, showing a limited effect of the toxin on the NO pathway. We found the genes sparc, ednrb, junb, cdkn1a, bcl2, ccl5, abcc1 over-expressed and the genes sod1, s100a10 and fth1 under-expressed after inoculation of Tx2-6. The differential expressions of sparc and ednrb genes were further confirmed using real-time PCR. Interestingly, ednrb activates the l-arginine/NO/cGMP pathway that is involved in the relaxation of the cavernous body. Therefore the priapism induced by Tx2-6 is a consequence of a highly specific interference of this neurotoxin with the NO pathway.
Fabiola E. Villanova; Enrico Andrade; Élcio Leal; Priscila M. Andrade; Ricardo C. Borra; Lanfranco R.P. Troncone; Leandro Magalhães; Katia R.M. Leite; Mario Paranhos; Joaquim Claro; Miguel Srougi. Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice. Toxicon 2009, 54, 793 -801.
AMA StyleFabiola E. Villanova, Enrico Andrade, Élcio Leal, Priscila M. Andrade, Ricardo C. Borra, Lanfranco R.P. Troncone, Leandro Magalhães, Katia R.M. Leite, Mario Paranhos, Joaquim Claro, Miguel Srougi. Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice. Toxicon. 2009; 54 (6):793-801.
Chicago/Turabian StyleFabiola E. Villanova; Enrico Andrade; Élcio Leal; Priscila M. Andrade; Ricardo C. Borra; Lanfranco R.P. Troncone; Leandro Magalhães; Katia R.M. Leite; Mario Paranhos; Joaquim Claro; Miguel Srougi. 2009. "Erection induced by Tx2-6 toxin of Phoneutria nigriventer spider: Expression profile of genes in the nitric oxide pathway of penile tissue of mice." Toxicon 54, no. 6: 793-801.
Since successful treatment of superficial bladder cancer with BCG requires proper induction of Th1 immunity, we have developed a rBCG-S1PT strain that induced a stronger cellular immune response than BCG. This preclinical study was designed to compare the modulatory effects of BCG and rBCG-S1PT on bladder TNF-α and IL-10 expression and to evaluate antitumour activity.
Daher C Chade; Ricardo Carneiro Borra; Ivan Nascimento; Fabiola E Villanova; Luciana Cc Leite; Enrico Fm Andrade; Miguel Srougi; Kátia L Ramos; Priscila M Andrade. Immunomodulatory effects of recombinant BCG expressing pertussis toxin on TNF-alpha and IL-10 in a bladder cancer model. Journal of Experimental & Clinical Cancer Research 2008, 27, 78 -6.
AMA StyleDaher C Chade, Ricardo Carneiro Borra, Ivan Nascimento, Fabiola E Villanova, Luciana Cc Leite, Enrico Fm Andrade, Miguel Srougi, Kátia L Ramos, Priscila M Andrade. Immunomodulatory effects of recombinant BCG expressing pertussis toxin on TNF-alpha and IL-10 in a bladder cancer model. Journal of Experimental & Clinical Cancer Research. 2008; 27 (1):78-6.
Chicago/Turabian StyleDaher C Chade; Ricardo Carneiro Borra; Ivan Nascimento; Fabiola E Villanova; Luciana Cc Leite; Enrico Fm Andrade; Miguel Srougi; Kátia L Ramos; Priscila M Andrade. 2008. "Immunomodulatory effects of recombinant BCG expressing pertussis toxin on TNF-alpha and IL-10 in a bladder cancer model." Journal of Experimental & Clinical Cancer Research 27, no. 1: 78-6.
Our purpose, in the present work, was to further comprehend the genetic events underlying the response to steroids of human endometrium from the mRNA as well as protein expression point of view. In order to achieve this goal we undertook 10 000-oligonucleotide, three-dimensional microarray analysis, followed by immunohistochemistry, on human normal endometrium in the proliferative and secretory phases of the menstrual cycle. The results revealed that a myriad of genes involved in immune response, calcium metabolism and thyroid hormone response were frequently overexpressed in the second or luteal phase of the menstrual cycle. During the follicular phase, in contrast, overexpression of genes was mainly restricted to those encoding proteins involved in cell proliferation.
Audrey Y. Otsuka; Priscila M. Andrade; Fabiola E. Villanova; Ricardo Carneiro Borra; Ismael D. C. G. Silva. Human endometrium mRNA profile assessed by oligonucleotide three-dimensional microarray. Gynecological Endocrinology 2007, 23, 527 -534.
AMA StyleAudrey Y. Otsuka, Priscila M. Andrade, Fabiola E. Villanova, Ricardo Carneiro Borra, Ismael D. C. G. Silva. Human endometrium mRNA profile assessed by oligonucleotide three-dimensional microarray. Gynecological Endocrinology. 2007; 23 (9):527-534.
Chicago/Turabian StyleAudrey Y. Otsuka; Priscila M. Andrade; Fabiola E. Villanova; Ricardo Carneiro Borra; Ismael D. C. G. Silva. 2007. "Human endometrium mRNA profile assessed by oligonucleotide three-dimensional microarray." Gynecological Endocrinology 23, no. 9: 527-534.
Uterine leiomyoma is the most frequent pelvic tumor found in female genital tract. Some studies have suggested an association between single nucleotide polymorphisms (SNPs) in estrogen receptors genes with susceptibility in developing uterine leiomyoma. In this work, we estimated the frequency of two SNPs: one located in the intron 1 (rs9322331) and other in the exon 1 (rs17847075) of the estrogen receptor α (ESR1) gene in 125 women with uterine leiomyoma and 125 healthy women. To do this we used a PCR–RFLP method with MspI and HaeIII restriction enzymes to respectively detect C/T SNPs in the intron 1 and in the exon 1 of ESR1. To our knowledge this is the first study aimed to investigate the association of ESR1 SNPs with the risk of developing uterine leiomyoma in Brazilian women. Our results showed that the allele frequencies of the exon 1 and the intron 1 of the ESR1 gene did not differ between cases and controls (P = 0.325 and 0.175, respectively). Furthermore, our findings provided little support for the association of these SNPs on ESR1 with leiomyoma. However, we found that the SNP in the intron 1 of the ESR1 gene was underrepresented in the Brazilian female population.
Fabiola E. Villanova; Priscila M. Andrade; Audrey Y. Otsuka; Mariano T.V. Gomes; Elcio Leal; Rodrigo A. Castro; Manoel J.B.C. Girão; Eddy Nishimura; Edmund C. Baracat; Ismael D.C.G. Silva. Estrogen receptor alpha polymorphism and susceptibility to uterine leiomyoma. Steroids 2006, 71, 960 -965.
AMA StyleFabiola E. Villanova, Priscila M. Andrade, Audrey Y. Otsuka, Mariano T.V. Gomes, Elcio Leal, Rodrigo A. Castro, Manoel J.B.C. Girão, Eddy Nishimura, Edmund C. Baracat, Ismael D.C.G. Silva. Estrogen receptor alpha polymorphism and susceptibility to uterine leiomyoma. Steroids. 2006; 71 (11):960-965.
Chicago/Turabian StyleFabiola E. Villanova; Priscila M. Andrade; Audrey Y. Otsuka; Mariano T.V. Gomes; Elcio Leal; Rodrigo A. Castro; Manoel J.B.C. Girão; Eddy Nishimura; Edmund C. Baracat; Ismael D.C.G. Silva. 2006. "Estrogen receptor alpha polymorphism and susceptibility to uterine leiomyoma." Steroids 71, no. 11: 960-965.