The process of micronization of levofloxacin (LF, an antibacterial agent of the fluoroquinolone group) by the supercritical antisolvent precipitation technique (SAS) was investigated. It was shown that LF particles of different sizes (from 1 to 10 μm) and of various morphologies (from thin plates to elongated parallelepipeds) can be produced depending on the type of solvent used for conducting micronization. Investigation of the micronized LF preparations using the methods of IR-Fourier spectroscopy, Raman scattering, and circular dichroism showed that the LF micronization caused neither changes in its chemical structure nor racemization. Micronization of LF significantly affects the rate of its dissolution in model systems exhibiting effects dependent on the type of the solvent used for micronization. For example, the highest rate of dissolution at pH 4 was observed for LF preparations micronized with the help of chlorohydrocarbons. It was shown that the rate of dissolution of all micronized LF preparations was higher by 15–30% in comparison with the initial LF, which likely was related to the changes in the degree of crystallinity/amorphousness, as well as of morphologies of microparticles formed in the SAS process.

In this study, the nanoscale transformation of the polylactic-co-glycolic acid (PLGA) internal structure, before and after its supercritical carbon dioxide (sc-CO₂) swelling and plasticization, followed by foaming after a CO₂ pressure drop, was studied by small-angle X-ray scattering (SAXS) for the first time. A comparative analysis of the internal structure data and porosity measurements for PLGA scaffolds, produced by sc-CO₂ processing, on a scale ranging from 0.02 to 1000 μm, was performed by SAXS, helium pycnometry (HP), mercury intrusion porosimetry (MIP) and both “lab-source” and synchrotron X-ray microtomography (micro-CT). This approach opens up possibilities for the wide-scale evaluation, computer modeling, and prediction of the physical and mechanical properties of PLGA scaffolds, as well as their biodegradation behavior in the body. Hence, this study targets optimizing the process parameters of PLGA scaffold fabrication for specific biomedical applications.

The processes of hydrophilization of the initially hydrophobic surfaces of monolithic disks from D,L-polylactide and polylactoglycolide used to create a variety of tissue-engineered structures based on them are studied. The surface of the studied samples is processed by the method of directed hydrolytic modification, by means of dielectric discharge plasma, by the method of deposition of biocompatible hydrophilic compounds (BHCs) on the surface, or by the method of preliminary introduction of the BHCs into the material of the disk and hydrolytic modification of its surface. Sodium alginate, maltodextrin, and hyaluronic acid are used in experiments as BHCs. A comparative analysis of the contact angles of wetting between the surfaces of the initial disks and treated disks shows that the most hydrophilic surface is the surface of the samples obtained from a powder mixture of aliphatic polyesters with sodium alginate after hydrolytic modification.