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Hyo-Jun Lee
Department of Functional Genomics, KRIBB School of Biotechnology, Korea University of Science and Technology, Daejeon 34113, Korea

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Short Biography

My research interest is plant responses to external signals. During the PhD course, I have studied plant behaviors and underlying molecular mechanisms at high temperatures using Arabidopsis. Recently, as a senior researcher in KRIBB, I have started to study plant responses to mechanical stimulation such as touch and wound. In addition, I am studying plant regeneration processes using crops including potato and tomato.

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Journal article
Published: 22 August 2021 in International Journal of Molecular Sciences
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OsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the UsnRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice.

ACS Style

Hyun-Ji Park; Hae-Myeong Jung; Areum Lee; Seung-Hee Jo; Hyo-Jun Lee; Hyun-Soon Kim; Choon-Kyun Jung; Sung-Ran Min; Hye-Sun Cho. SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice. International Journal of Molecular Sciences 2021, 22, 9049 .

AMA Style

Hyun-Ji Park, Hae-Myeong Jung, Areum Lee, Seung-Hee Jo, Hyo-Jun Lee, Hyun-Soon Kim, Choon-Kyun Jung, Sung-Ran Min, Hye-Sun Cho. SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice. International Journal of Molecular Sciences. 2021; 22 (16):9049.

Chicago/Turabian Style

Hyun-Ji Park; Hae-Myeong Jung; Areum Lee; Seung-Hee Jo; Hyo-Jun Lee; Hyun-Soon Kim; Choon-Kyun Jung; Sung-Ran Min; Hye-Sun Cho. 2021. "SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice." International Journal of Molecular Sciences 22, no. 16: 9049.

Original research article
Published: 15 July 2021 in Frontiers in Plant Science
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Plants absorb light energy required for photosynthesis, but excess light can damage plant cells. To protect themselves, plants have developed diverse signaling pathways which are activated under high-intensity light. Plant photoprotection mechanisms have been mainly investigated under conditions of extremely high amount of light; thus, it is largely unknown how plants manage photooxidative damage under moderate light intensities. In the present study, we found that FERONIA (FER) is a key protein that confers resistance to photooxidative stress in plants under moderate light intensity. FER-deficient mutants were highly susceptible to increasing light intensity and exhibited photobleaching even under moderately elevated light intensity (ML). Light-induced expression of stress genes was largely diminished by the fer-4 mutation. In addition, excitation pressure on Photosystem II was significantly increased in fer-4 mutants under ML. Consistently, reactive oxygen species, particularly singlet oxygen, accumulated in fer-4 mutants grown under ML. FER protein abundance was found to be elevated after exposure to ML, which is indirectly affected by the ubiquitin-proteasome pathway. Altogether, our findings showed that plants require FER-mediated photoprotection to maintain their photosystems even under moderate light intensity.

ACS Style

Seung Yong Shin; Ji-Sun Park; Hye-Bin Park; Ki-Beom Moon; Hyun-Soon Kim; Jae-Heung Jeon; Hye Sun Cho; Hyo-Jun Lee. FERONIA Confers Resistance to Photooxidative Stress in Arabidopsis. Frontiers in Plant Science 2021, 12, 1 .

AMA Style

Seung Yong Shin, Ji-Sun Park, Hye-Bin Park, Ki-Beom Moon, Hyun-Soon Kim, Jae-Heung Jeon, Hye Sun Cho, Hyo-Jun Lee. FERONIA Confers Resistance to Photooxidative Stress in Arabidopsis. Frontiers in Plant Science. 2021; 12 ():1.

Chicago/Turabian Style

Seung Yong Shin; Ji-Sun Park; Hye-Bin Park; Ki-Beom Moon; Hyun-Soon Kim; Jae-Heung Jeon; Hye Sun Cho; Hyo-Jun Lee. 2021. "FERONIA Confers Resistance to Photooxidative Stress in Arabidopsis." Frontiers in Plant Science 12, no. : 1.

Journal article
Published: 27 May 2021 in International Journal of Molecular Sciences
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Sound waves affect plants at the biochemical, physical, and genetic levels. However, the mechanisms by which plants respond to sound waves are largely unknown. Therefore, the aim of this study was to examine the effect of sound waves on Arabidopsis thaliana growth. The results of the study showed that Arabidopsis seeds exposed to sound waves (100 and 100 + 9k Hz) for 15 h per day for 3 day had significantly longer root growth than that in the control group. The root length and cell number in the root apical meristem were significantly affected by sound waves. Furthermore, genes involved in cell division were upregulated in seedlings exposed to sound waves. Root development was affected by the concentration and activity of some phytohormones, including cytokinin and auxin. Analysis of the expression levels of genes regulating cytokinin and auxin biosynthesis and signaling showed that cytokinin and ethylene signaling genes were downregulated, while auxin signaling and biosynthesis genes were upregulated in Arabidopsis exposed to sound waves. Additionally, the cytokinin and auxin concentrations of the roots of Arabidopsis plants increased and decreased, respectively, after exposure to sound waves. Our findings suggest that sound waves are potential agricultural tools for improving crop growth performance.

ACS Style

Joo Kim; Hyo-Jun Lee; Jin Kim; Mi-Jeong Jeong. Sound Waves Promote Arabidopsis thaliana Root Growth by Regulating Root Phytohormone Content. International Journal of Molecular Sciences 2021, 22, 5739 .

AMA Style

Joo Kim, Hyo-Jun Lee, Jin Kim, Mi-Jeong Jeong. Sound Waves Promote Arabidopsis thaliana Root Growth by Regulating Root Phytohormone Content. International Journal of Molecular Sciences. 2021; 22 (11):5739.

Chicago/Turabian Style

Joo Kim; Hyo-Jun Lee; Jin Kim; Mi-Jeong Jeong. 2021. "Sound Waves Promote Arabidopsis thaliana Root Growth by Regulating Root Phytohormone Content." International Journal of Molecular Sciences 22, no. 11: 5739.

Journal article
Published: 28 April 2021 in International Journal of Molecular Sciences
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Precise flowering timing is critical for the plant life cycle. Here, we examined the molecular mechanisms and regulatory network associated with flowering in Chinese cabbage (Brassica rapa L.) by comparative transcriptome profiling of two Chinese cabbage inbred lines, “4004” (early bolting) and “50” (late bolting). RNA-Seq and quantitative reverse transcription PCR (qPCR) analyses showed that two positive nitric oxide (NO) signaling regulator genes, nitrite reductase (BrNIR) and nitrate reductase (BrNIA), were up-regulated in line “50” with or without vernalization. In agreement with the transcription analysis, the shoots in line “50” had substantially higher nitrogen levels than those in “4004”. Upon vernalization, the flowering repressor gene Circadian 1 (BrCIR1) was significantly up-regulated in line “50”, whereas the flowering enhancer genes named SUPPRESSOR OF OVEREXPRESSION OF CONSTANCE 1 homologs (BrSOC1s) were substantially up-regulated in line “4004”. CRISPR/Cas9-mediated mutagenesis in Chinese cabbage demonstrated that the BrSOC1-1/1-2/1-3 genes were involved in late flowering, and their expression was mutually exclusive with that of the nitrogen signaling genes. Thus, we identified two flowering mechanisms in Chinese cabbage: a reciprocal negative feedback loop between nitrogen signaling genes (BrNIA1 and BrNIR1) and BrSOC1s to control flowering time and positive feedback control of the expression of BrSOC1s.

ACS Style

Haemyeong Jung; Areum Lee; Seung Jo; Hyun Park; Won Jung; Hyun-Soon Kim; Hyo-Jun Lee; Seon-Geum Jeong; Youn-Sung Kim; Hye Cho. Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs. International Journal of Molecular Sciences 2021, 22, 4631 .

AMA Style

Haemyeong Jung, Areum Lee, Seung Jo, Hyun Park, Won Jung, Hyun-Soon Kim, Hyo-Jun Lee, Seon-Geum Jeong, Youn-Sung Kim, Hye Cho. Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs. International Journal of Molecular Sciences. 2021; 22 (9):4631.

Chicago/Turabian Style

Haemyeong Jung; Areum Lee; Seung Jo; Hyun Park; Won Jung; Hyun-Soon Kim; Hyo-Jun Lee; Seon-Geum Jeong; Youn-Sung Kim; Hye Cho. 2021. "Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs." International Journal of Molecular Sciences 22, no. 9: 4631.

Journal article
Published: 16 April 2021 in Plants
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Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.

ACS Style

Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants 2021, 10, 781 .

AMA Style

Ki-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants. 2021; 10 (4):781.

Chicago/Turabian Style

Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. 2021. "A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts." Plants 10, no. 4: 781.

Journal article
Published: 06 November 2020 in Communications Biology
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Flooding is a common and critical disaster in agriculture, because it causes defects in plant growth and even crop loss. An increase in herbivore populations is often observed after floods, which leads to additional damage to the plants. Although molecular mechanisms underlying the plant responses to flooding have been identified, how plant defence systems are affected by flooding remains poorly understood. Herein, we show that submergence deactivates wound-induced defence against herbivore attack in Arabidopsis thaliana. Submergence rapidly suppressed the wound-induced expression of jasmonic acid (JA) biosynthesis genes, resulting in reduced JA accumulation. While plants exposed to hypoxia in argon gas exhibited similar reduced wound responses, the inhibitory effects were initiated after short-term submergence without signs for lack of oxygen. Instead, expression of ethylene-responsive genes was increased after short-term submergence. Blocking ethylene signalling by ein2-1 mutation partially restored suppressed expression of several wound-responsive genes by submergence. In addition, submergence rapidly removed active markers of histone modifications at a gene locus involved in JA biosynthesis. Our findings suggest that submergence inactivates defence systems of plants, which would explain the proliferation of herbivores after flooding.

ACS Style

Hyo-Jun Lee; Ji-Sun Park; Seung Yong Shin; Sang-Gyu Kim; Gisuk Lee; Hyun-Soon Kim; Jae Heung Jeon; Hye Sun Cho. Submergence deactivates wound-induced plant defence against herbivores. Communications Biology 2020, 3, 1 -9.

AMA Style

Hyo-Jun Lee, Ji-Sun Park, Seung Yong Shin, Sang-Gyu Kim, Gisuk Lee, Hyun-Soon Kim, Jae Heung Jeon, Hye Sun Cho. Submergence deactivates wound-induced plant defence against herbivores. Communications Biology. 2020; 3 (1):1-9.

Chicago/Turabian Style

Hyo-Jun Lee; Ji-Sun Park; Seung Yong Shin; Sang-Gyu Kim; Gisuk Lee; Hyun-Soon Kim; Jae Heung Jeon; Hye Sun Cho. 2020. "Submergence deactivates wound-induced plant defence against herbivores." Communications Biology 3, no. 1: 1-9.

Journal article
Published: 28 May 2020 in Plants
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Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.

ACS Style

Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688 .

AMA Style

Ji-Sun Park, Zuh-Kyung Seong, Mi-Sun Kim, Jang-Ho Ha, Ki-Beom Moon, Hyo-Jun Lee, Hyeong-Kyu Lee, Jae-Heung Jeon, Sang Un Park, Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants. 2020; 9 (6):688.

Chicago/Turabian Style

Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. 2020. "Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton." Plants 9, no. 6: 688.

Review
Published: 24 December 2019 in Plants
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Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.

ACS Style

Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants 2019, 9, 30 .

AMA Style

Ki-Beom Moon, Ji-Sun Park, Youn-Il Park, In-Ja Song, Hyo-Jun Lee, Hye Sun Cho, Jae-Heung Jeon, Hyun-Soon Kim, Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants. 2019; 9 (1):30.

Chicago/Turabian Style

Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. 2019. "Development of Systems for the Production of Plant-Derived Biopharmaceuticals." Plants 9, no. 1: 30.

Regular article
Published: 23 July 2019 in New Phytologist
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Plants sense mechanical stimuli to recognize nearby obstacles and change their growth patterns to adapt to the surrounding environment. When roots encounter an obstacle, they rapidly bend away from the impenetrable surface and find the edge of the barrier. However, the molecular mechanisms underlying root obstacle avoidance are largely unknown. Here, we demonstrate that PIN‐FORMED (PIN)‐mediated polar auxin transport facilitates root bending during obstacle avoidance. We analyzed two types of bending after roots touched barriers. In auxin receptor mutants, the rate of root movement during first bending was largely delayed. Gravity‐oriented second bending was also disturbed in these mutants. The reporter assays showed that asymmetrical auxin responses occurred in the roots during obstacle avoidance. Pharmacological analysis suggested that polar auxin transport mediates local auxin accumulation. We found that PINs are required for auxin‐assisted root bending during obstacle avoidance. We propose that rapid root movement during obstacle avoidance is not just a passive but an active bending completed through polar auxin transport. Our findings suggest that auxin plays a role in thigmotropism during plant‐obstacle interactions. This article is protected by copyright. All rights reserved.

ACS Style

Hyo‐Jun Lee; Hyun‐Soon Kim; Jeong Mee Park; Hye Sun Cho; Jae Heung Jeon. PIN ‐mediated polar auxin transport facilitates root−obstacle avoidance. New Phytologist 2019, 225, 1285 -1296.

AMA Style

Hyo‐Jun Lee, Hyun‐Soon Kim, Jeong Mee Park, Hye Sun Cho, Jae Heung Jeon. PIN ‐mediated polar auxin transport facilitates root−obstacle avoidance. New Phytologist. 2019; 225 (3):1285-1296.

Chicago/Turabian Style

Hyo‐Jun Lee; Hyun‐Soon Kim; Jeong Mee Park; Hye Sun Cho; Jae Heung Jeon. 2019. "PIN ‐mediated polar auxin transport facilitates root−obstacle avoidance." New Phytologist 225, no. 3: 1285-1296.