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Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.
Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants 2021, 10, 781 .
AMA StyleKi-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants. 2021; 10 (4):781.
Chicago/Turabian StyleKi-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. 2021. "A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts." Plants 10, no. 4: 781.
Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.
Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688 .
AMA StyleJi-Sun Park, Zuh-Kyung Seong, Mi-Sun Kim, Jang-Ho Ha, Ki-Beom Moon, Hyo-Jun Lee, Hyeong-Kyu Lee, Jae-Heung Jeon, Sang Un Park, Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants. 2020; 9 (6):688.
Chicago/Turabian StyleJi-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. 2020. "Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton." Plants 9, no. 6: 688.
Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.
Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants 2019, 9, 30 .
AMA StyleKi-Beom Moon, Ji-Sun Park, Youn-Il Park, In-Ja Song, Hyo-Jun Lee, Hye Sun Cho, Jae-Heung Jeon, Hyun-Soon Kim, Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants. 2019; 9 (1):30.
Chicago/Turabian StyleKi-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. 2019. "Development of Systems for the Production of Plant-Derived Biopharmaceuticals." Plants 9, no. 1: 30.