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OsFKBP20-1b, a plant-specific cyclophilin protein, has been implicated to regulate pre-mRNA splicing under stress conditions in rice. Here, we demonstrated that OsFKBP20-1b is SUMOylated in a reconstituted SUMOylation system in E.coli and in planta, and that the SUMOylation-coupled regulation was associated with enhanced protein stability using a less SUMOylated OsFKBP20-1b mutant (5KR_OsFKBP20-1b). Furthermore, OsFKBP20-1b directly interacted with OsSUMO1 and OsSUMO2 in the nucleus and cytoplasm, whereas the less SUMOylated 5KR_OsFKBP20-1b mutant had an impaired interaction with OsSUMO1 and 2 in the cytoplasm but not in the nucleus. Under heat stress, the abundance of an OsFKBP20-1b-GFP fusion protein was substantially increased in the nuclear speckles and cytoplasmic foci, whereas the heat-responsiveness was remarkably diminished in the presence of the less SUMOylated 5KR_OsFKBP20-1b-GFP mutant. The accumulation of endogenous SUMOylated OsFKBP20-1b was enhanced by heat stress in planta. Moreover, 5KR_OsFKBP20-1b was not sufficiently associated with the UsnRNAs in the nucleus as a spliceosome component. A protoplast transfection assay indicated that the low SUMOylation level of 5KR_OsFKBP20-1b led to inaccurate alternative splicing and transcription under heat stress. Thus, our results suggest that OsFKBP20-1b is post-translationally regulated by SUMOylation, and the modification is crucial for proper RNA processing in response to heat stress in rice.
Hyun-Ji Park; Hae-Myeong Jung; Areum Lee; Seung-Hee Jo; Hyo-Jun Lee; Hyun-Soon Kim; Choon-Kyun Jung; Sung-Ran Min; Hye-Sun Cho. SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice. International Journal of Molecular Sciences 2021, 22, 9049 .
AMA StyleHyun-Ji Park, Hae-Myeong Jung, Areum Lee, Seung-Hee Jo, Hyo-Jun Lee, Hyun-Soon Kim, Choon-Kyun Jung, Sung-Ran Min, Hye-Sun Cho. SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice. International Journal of Molecular Sciences. 2021; 22 (16):9049.
Chicago/Turabian StyleHyun-Ji Park; Hae-Myeong Jung; Areum Lee; Seung-Hee Jo; Hyo-Jun Lee; Hyun-Soon Kim; Choon-Kyun Jung; Sung-Ran Min; Hye-Sun Cho. 2021. "SUMO Modification of OsFKBP20-1b Is Integral to Proper Pre-mRNA Splicing upon Heat Stress in Rice." International Journal of Molecular Sciences 22, no. 16: 9049.
Precise flowering timing is critical for the plant life cycle. Here, we examined the molecular mechanisms and regulatory network associated with flowering in Chinese cabbage (Brassica rapa L.) by comparative transcriptome profiling of two Chinese cabbage inbred lines, “4004” (early bolting) and “50” (late bolting). RNA-Seq and quantitative reverse transcription PCR (qPCR) analyses showed that two positive nitric oxide (NO) signaling regulator genes, nitrite reductase (BrNIR) and nitrate reductase (BrNIA), were up-regulated in line “50” with or without vernalization. In agreement with the transcription analysis, the shoots in line “50” had substantially higher nitrogen levels than those in “4004”. Upon vernalization, the flowering repressor gene Circadian 1 (BrCIR1) was significantly up-regulated in line “50”, whereas the flowering enhancer genes named SUPPRESSOR OF OVEREXPRESSION OF CONSTANCE 1 homologs (BrSOC1s) were substantially up-regulated in line “4004”. CRISPR/Cas9-mediated mutagenesis in Chinese cabbage demonstrated that the BrSOC1-1/1-2/1-3 genes were involved in late flowering, and their expression was mutually exclusive with that of the nitrogen signaling genes. Thus, we identified two flowering mechanisms in Chinese cabbage: a reciprocal negative feedback loop between nitrogen signaling genes (BrNIA1 and BrNIR1) and BrSOC1s to control flowering time and positive feedback control of the expression of BrSOC1s.
Haemyeong Jung; Areum Lee; Seung Jo; Hyun Park; Won Jung; Hyun-Soon Kim; Hyo-Jun Lee; Seon-Geum Jeong; Youn-Sung Kim; Hye Cho. Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs. International Journal of Molecular Sciences 2021, 22, 4631 .
AMA StyleHaemyeong Jung, Areum Lee, Seung Jo, Hyun Park, Won Jung, Hyun-Soon Kim, Hyo-Jun Lee, Seon-Geum Jeong, Youn-Sung Kim, Hye Cho. Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs. International Journal of Molecular Sciences. 2021; 22 (9):4631.
Chicago/Turabian StyleHaemyeong Jung; Areum Lee; Seung Jo; Hyun Park; Won Jung; Hyun-Soon Kim; Hyo-Jun Lee; Seon-Geum Jeong; Youn-Sung Kim; Hye Cho. 2021. "Nitrogen Signaling Genes and SOC1 Determine the Flowering Time in a Reciprocal Negative Feedback Loop in Chinese Cabbage (Brassica rapa L.) Based on CRISPR/Cas9-Mediated Mutagenesis of Multiple BrSOC1 Homologs." International Journal of Molecular Sciences 22, no. 9: 4631.
Both obtaining high-yielding, viable protoplasts and following reliable regeneration protocols are prerequisites for the continuous expansion and development of newly emerging systems involving protoplast utilization. This study determines an efficient process from protoplast isolation to shoot regeneration in vitro. The maximum yield of protoplast extraction, which was 6.36 ± 0.51 × 106 protoplasts/g fresh weight (FW), was approximately 3.7 times higher than that previously reported for potato protoplasts. To obtain data, wounded leaves were used by partially cutting both sides of the midrib, and isolated protoplasts were purified by the sucrose cushion method, with a sucrose concentration of 20%. We confirmed a significant effect on the extraction efficiency by measuring enzymolysis during a 6 h period, with three times more washing buffer than the amount normally used. Protoplasts fixed in alginate lenses with appropriate space were successfully recovered and developed into microcalli 2 weeks after culture. In addition, to induce high efficiency regeneration from protoplasts, calli in which greening occurred for 6 weeks were induced to develop shoots in regeneration medium solidified by Gelrite, and they presented a high regeneration efficiency of 86.24 ± 11.76%.
Ki-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants 2021, 10, 781 .
AMA StyleKi-Beom Moon, Ji-Sun Park, Su-Jin Park, Hyo-Jun Lee, Hye-Sun Cho, Sung-Ran Min, Youn-Il Park, Jae-Heung Jeon, Hyun-Soon Kim. A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts. Plants. 2021; 10 (4):781.
Chicago/Turabian StyleKi-Beom Moon; Ji-Sun Park; Su-Jin Park; Hyo-Jun Lee; Hye-Sun Cho; Sung-Ran Min; Youn-Il Park; Jae-Heung Jeon; Hyun-Soon Kim. 2021. "A More Accessible, Time-Saving, and Efficient Method for In Vitro Plant Regeneration from Potato Protoplasts." Plants 10, no. 4: 781.
Pterocarpans are derivatives of isoflavonoids, found in many species of the family Fabaceae. Sophora flavescens Aiton is a promising traditional Asian medicinal plant. Plant cell suspension cultures represent an excellent source for the production of valuable secondary metabolites. Herein, we found that methyl jasmonate (MJ) elicited the activation of pterocarpan biosynthetic genes in cell suspension cultures of S. flavescens and enhanced the accumulation of pterocarpans, producing mainly trifolirhizin, trifolirhizin malonate, and maackiain. MJ application stimulated the expression of structural genes (PAL, C4H, 4CL, CHS, CHR, CHI, IFS, I3’H, and IFR) of the pterocarpan biosynthetic pathway. In addition, the co-treatment of MJ and methyl-β-cyclodextrin (MeβCD) as a solubilizer exhibited a synergistic effect on the activation of the pterocarpan biosynthetic genes. The maximum level of total pterocarpan production (37.2 mg/g dry weight (DW)) was obtained on day 17 after the application of 50 μM MJ on cells. We also found that the combined treatment of cells for seven days with MJ and MeβCD synergistically induced the pterocarpan production (trifolirhizin, trifolirhizin malonate, and maackiain) in the cells (58 mg/g DW) and culture medium (222.7 mg/L). Noteworthy, the co-treatment only stimulated the elevated extracellular production of maackiain in the culture medium, indicating its extracellular secretion; however, its glycosides (trifolirhizin and trifolirhizin malonate) were not detected in any significant amounts in the culture medium. This work provides new strategies for the pterocarpan production in plant cell suspension cultures, and shows MeβCD to be an effective solubilizer for the extracellular production of maackiain in the cell cultures of S. flavescens.
Soyoung Kim; Yu Jeong Jeong; Su Hyun Park; Sung-Chul Park; Saet Buyl Lee; Jiyoung Lee; Suk Weon Kim; Bo-Keun Ha; Hyun-Soon Kim; Hyeran Kim; Young Bae Ryu; Jae Cheol Jeong; Cha Young Kim. The Synergistic Effect of Co-Treatment of Methyl Jasmonate and Cyclodextrins on Pterocarpan Production in Sophora flavescens Cell Cultures. International Journal of Molecular Sciences 2020, 21, 3944 .
AMA StyleSoyoung Kim, Yu Jeong Jeong, Su Hyun Park, Sung-Chul Park, Saet Buyl Lee, Jiyoung Lee, Suk Weon Kim, Bo-Keun Ha, Hyun-Soon Kim, Hyeran Kim, Young Bae Ryu, Jae Cheol Jeong, Cha Young Kim. The Synergistic Effect of Co-Treatment of Methyl Jasmonate and Cyclodextrins on Pterocarpan Production in Sophora flavescens Cell Cultures. International Journal of Molecular Sciences. 2020; 21 (11):3944.
Chicago/Turabian StyleSoyoung Kim; Yu Jeong Jeong; Su Hyun Park; Sung-Chul Park; Saet Buyl Lee; Jiyoung Lee; Suk Weon Kim; Bo-Keun Ha; Hyun-Soon Kim; Hyeran Kim; Young Bae Ryu; Jae Cheol Jeong; Cha Young Kim. 2020. "The Synergistic Effect of Co-Treatment of Methyl Jasmonate and Cyclodextrins on Pterocarpan Production in Sophora flavescens Cell Cultures." International Journal of Molecular Sciences 21, no. 11: 3944.
Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established.
Ji-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688 .
AMA StyleJi-Sun Park, Zuh-Kyung Seong, Mi-Sun Kim, Jang-Ho Ha, Ki-Beom Moon, Hyo-Jun Lee, Hyeong-Kyu Lee, Jae-Heung Jeon, Sang Un Park, Hyun-Soon Kim. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants. 2020; 9 (6):688.
Chicago/Turabian StyleJi-Sun Park; Zuh-Kyung Seong; Mi-Sun Kim; Jang-Ho Ha; Ki-Beom Moon; Hyo-Jun Lee; Hyeong-Kyu Lee; Jae-Heung Jeon; Sang Un Park; Hyun-Soon Kim. 2020. "Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton." Plants 9, no. 6: 688.
Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.
Ki-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants 2019, 9, 30 .
AMA StyleKi-Beom Moon, Ji-Sun Park, Youn-Il Park, In-Ja Song, Hyo-Jun Lee, Hye Sun Cho, Jae-Heung Jeon, Hyun-Soon Kim, Park. Development of Systems for the Production of Plant-Derived Biopharmaceuticals. Plants. 2019; 9 (1):30.
Chicago/Turabian StyleKi-Beom Moon; Ji-Sun Park; Youn-Il Park; In-Ja Song; Hyo-Jun Lee; Hye Sun Cho; Jae-Heung Jeon; Hyun-Soon Kim; Park. 2019. "Development of Systems for the Production of Plant-Derived Biopharmaceuticals." Plants 9, no. 1: 30.
Carbohydrates are the primary energy source for plant development. Plants synthesize sucrose in source organs and transport them to sink organs during plant growth. This metabolism is sensitive to environmental changes in light quantity, quality, and photoperiod. In the daytime, the synthesis of sucrose and starch accumulates, and starch is degraded at nighttime. The circadian clock genes provide plants with information on the daily environmental changes and directly control many developmental processes, which are related to the path of primary metabolites throughout the life cycle. The circadian clock mechanism and processes of metabolism controlled by the circadian rhythm were studied in the model plant Arabidopsis and in the crops potato and rice. However, the translation of molecular mechanisms obtained from studies of model plants to crop plants is still difficult. Crop plants have specific organs such as edible seed and tuber that increase the size or accumulate valuable metabolites by harvestable metabolic components. Human consumers are interested in the regulation and promotion of these agriculturally significant crops. Circadian clock manipulation may suggest various strategies for the increased productivity of food crops through using environmental signal or overcoming environmental stress.
Jin A Kim; Hyun-Soon Kim; Seo-Hwa Choi; Ji-Young Jang; Mi-Jeong Jeong; Soo In Lee. The Importance of the Circadian Clock in Regulating Plant Metabolism. International Journal of Molecular Sciences 2017, 18, 2680 .
AMA StyleJin A Kim, Hyun-Soon Kim, Seo-Hwa Choi, Ji-Young Jang, Mi-Jeong Jeong, Soo In Lee. The Importance of the Circadian Clock in Regulating Plant Metabolism. International Journal of Molecular Sciences. 2017; 18 (12):2680.
Chicago/Turabian StyleJin A Kim; Hyun-Soon Kim; Seo-Hwa Choi; Ji-Young Jang; Mi-Jeong Jeong; Soo In Lee. 2017. "The Importance of the Circadian Clock in Regulating Plant Metabolism." International Journal of Molecular Sciences 18, no. 12: 2680.
We previously isolated Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) from tobacco leaves. The NMMP1 gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant's defense against bacterial pathogens. Expression of NMMP1 was strongly induced during interactions between tobacco and one of its pathogens, Phytophthora infestans. To elucidate the role of the NMMP1 in defense of N. benthamiana against fungal pathogens, we performed gain-of-function and loss-of-function studies. NMMP1-overexpressing plants had stronger resistance responses against P. infestans infections than control plants, while silencing of NMMP1 resulted in greater susceptibility of the plants to the pathogen. This greater susceptibility correlated with fewer NMMP1 transcripts than the non-silenced control. We also examined cell death as a measure of disease. The amount of cell death induced by the necrosis-inducing P. infestans protein 1, PiNPP1, was dependent on NMMP1 in N. benthamiana. Potato plants overexpressing NMMP1 also had enhanced disease resistance against P. infestans. RT-PCR analysis of these transgenic potato plants revealed constitutive up-regulation of the potato defense gene NbPR5. NMMP1-overexpressing potato plants were taller and produced heavier tubers than control plants. We suggest a role for NMMP1in pathogen defense and development.
Jang Ho Ha; Hyun A. Jang; Ki-Beom Moon; Kwang Hyun Baek; Gyung Ja Choi; Doil Choi; Hye Sun Cho; Suk Yun Kwon; Jae-Heung Jeon; Sang-Keun Oh; Hyun-Soon Kim. Nicotiana benthamiana Matrix Metalloprotease 1 (NMMP1) gene confers disease resistance to Phytophthora infestans in tobacco and potato plants. Journal of Plant Physiology 2017, 218, 189 -195.
AMA StyleJang Ho Ha, Hyun A. Jang, Ki-Beom Moon, Kwang Hyun Baek, Gyung Ja Choi, Doil Choi, Hye Sun Cho, Suk Yun Kwon, Jae-Heung Jeon, Sang-Keun Oh, Hyun-Soon Kim. Nicotiana benthamiana Matrix Metalloprotease 1 (NMMP1) gene confers disease resistance to Phytophthora infestans in tobacco and potato plants. Journal of Plant Physiology. 2017; 218 ():189-195.
Chicago/Turabian StyleJang Ho Ha; Hyun A. Jang; Ki-Beom Moon; Kwang Hyun Baek; Gyung Ja Choi; Doil Choi; Hye Sun Cho; Suk Yun Kwon; Jae-Heung Jeon; Sang-Keun Oh; Hyun-Soon Kim. 2017. "Nicotiana benthamiana Matrix Metalloprotease 1 (NMMP1) gene confers disease resistance to Phytophthora infestans in tobacco and potato plants." Journal of Plant Physiology 218, no. : 189-195.
Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana. Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana. The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated N. benthamiana. The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. N. benthamiana-derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of Escherichia coli-derived recombinant human acidic fibroblast growth factor. Additionally, N. benthamiana-derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that N. benthamiana-derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging.
Jang-Ho Ha; Ha-Neul Kim; Ki-Beom Moon; Jae-Heung Jeon; Dai-Hyun Jung; Su-Jung Kim; Hugh S. Mason; Seo-Yeon Shin; Hyun-Soon Kim; Kyung-Mok Park. Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging. Planta Medica 2017, 83, 862 -869.
AMA StyleJang-Ho Ha, Ha-Neul Kim, Ki-Beom Moon, Jae-Heung Jeon, Dai-Hyun Jung, Su-Jung Kim, Hugh S. Mason, Seo-Yeon Shin, Hyun-Soon Kim, Kyung-Mok Park. Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging. Planta Medica. 2017; 83 (10):862-869.
Chicago/Turabian StyleJang-Ho Ha; Ha-Neul Kim; Ki-Beom Moon; Jae-Heung Jeon; Dai-Hyun Jung; Su-Jung Kim; Hugh S. Mason; Seo-Yeon Shin; Hyun-Soon Kim; Kyung-Mok Park. 2017. "Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging." Planta Medica 83, no. 10: 862-869.
Jerusalem artichoke (Helianthus tuberosus L.), a plant of the Asteraceae family, is widely cultivated for its multiple pharmacological properties and is being developed as a renewable feedstock, as well as a source of biofuels and biochemicals for industrial applications. Despite its nutritional benefits and economic potential, transcriptomic and genomic information is scarce. In the present study, we performed phenotypic characterization and RNA-Seq analysis of three Jerusalem artichoke cultivars, “Purple Jerusalem artichoke” (PJA), “Hindung Jerusalem artichoke” (HJA) and “Dafeng Jerusalem artichoke” (DJA). The cultivars exhibited obvious differences in their responses to drought, high salinity and oxidative stress, as well as morphological variations. The PJA cultivar had the highest concentration of anthocyanin, and the DJA cultivar had the strongest tolerance to environmental stresses among the three cultivars. Based on the three assembled transcriptomes, we identified 2435, 3283 and 3657 putative cultivar-specific transcripts from leaf and tuber tissues in cultivars PJA, HJA and DJA, respectively, which enlarges the pool of transcriptomes available for Jerusalem artichoke. We also detected 11,319, 13,190 and 12,717 potential cultivar-specific simple sequence repeats (SSRs) from the transcriptomic data for, PJA, HJA and DJA, respectively. In addition, five SSRs were identified as candidate molecular markers for cultivar identification, as determined by genomic PCR analysis. Our comparative RNA-Seq analysis and de novo transcriptome assemblies constitute a comprehensive transcriptome resource and provide essential sequence information for identifying Jerusalem artichoke cultivars. These results should therefore be useful for future gene discovery, molecular studies and agricultural improvement of this important non-model species.
Won Yong Jung; Sang Sook Lee; Hyun Ji Park; Chul Wook Kim; Suk-Yoon Kwon; Jae-Heung Jeon; Hyun-Soon Kim; Hye Sun Cho. Comparative transcriptome profiling and SSR marker identification in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenotypic variation. Plant Biotechnology Reports 2016, 10, 447 -461.
AMA StyleWon Yong Jung, Sang Sook Lee, Hyun Ji Park, Chul Wook Kim, Suk-Yoon Kwon, Jae-Heung Jeon, Hyun-Soon Kim, Hye Sun Cho. Comparative transcriptome profiling and SSR marker identification in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenotypic variation. Plant Biotechnology Reports. 2016; 10 (6):447-461.
Chicago/Turabian StyleWon Yong Jung; Sang Sook Lee; Hyun Ji Park; Chul Wook Kim; Suk-Yoon Kwon; Jae-Heung Jeon; Hyun-Soon Kim; Hye Sun Cho. 2016. "Comparative transcriptome profiling and SSR marker identification in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenotypic variation." Plant Biotechnology Reports 10, no. 6: 447-461.
Disease prevention through vaccination is considered to be the greatest contribution to public health over the past century. Every year more than 100 million children are vaccinated with the standard World Health Organization (WHO)-recommended vaccines including hepatitis B (HepB). HepB is the most serious type of liver infection caused by the hepatitis B virus (HBV), however, it can be prevented by currently available recombinant vaccine, which has an excellent record of safety and effectiveness. To date, recombinant vaccines are produced in many systems of bacteria, yeast, insect, and mammalian and plant cells. Among these platforms, the use of plant cells has received considerable attention in terms of intrinsic safety, scalability, and appropriate modification of target proteins. Research groups worldwide have attempted to develop more efficacious plant-derived vaccines for over 30 diseases, most frequently HepB and influenza. More inspiring, approximately 12 plant-made antigens have already been tested in clinical trials, with successful outcomes. In this study, the latest information from the last 10 years on plant-derived antigens, especially hepatitis B surface antigen, approaches are reviewed and breakthroughs regarding the weak points are also discussed.
Young Hee Joung; Se Hee Park; Ki-Beom Moon; Jae-Heung Jeon; Chang Won Choi; Hyun-Soon Kim. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B. International Journal of Molecular Sciences 2016, 17, 1715 .
AMA StyleYoung Hee Joung, Se Hee Park, Ki-Beom Moon, Jae-Heung Jeon, Chang Won Choi, Hyun-Soon Kim. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B. International Journal of Molecular Sciences. 2016; 17 (10):1715.
Chicago/Turabian StyleYoung Hee Joung; Se Hee Park; Ki-Beom Moon; Jae-Heung Jeon; Chang Won Choi; Hyun-Soon Kim. 2016. "The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B." International Journal of Molecular Sciences 17, no. 10: 1715.
Alternative splicing (AS) is an important molecular mechanism by which single genes can generate multiple mRNA isoforms. We reported previously that, in Oryza sativa, the cyclophilin 19-4 (OsCYP19-4.1) transcript was significantly upregulated in response to cold stress, and that transgenic plants were cold tolerant. Here we show that, under cold stress, OsCYP19-4 produces eight transcript variants by intron retention and exon skipping, resulting in production of four distinct protein isoforms. The OsCYP19-4 AS isoforms exhibited different cellular localizations in the epidermal cells: in contrast to OsCYP19-4.1, the OsCYP19-4.2 and OsCYP19-4.3 proteins were primarily targeted to guard and subsidiary cells, whereas OsCYP19-4.5, which consists largely of an endoplasmic reticulum (ER) targeting signal, was co-localized with the RFP-BiP marker in the ER. In OsCYP19-4.2, the key residues of the PPIase domain are altered; consistent with this, recombinant OsCYP19-4.2 had significantly lower PPIase activity than OsCYP19-4.1 in vitro. Specific protein-protein interactions between OsCYP19-4.2/3 and AtRCN1 were verified in yeast two-hybrid (Y2H) and bimolecular fluoresence complementation (BiFC assays), although the OsCYP19-4 isoforms could not bind each other. Based on these results, we propose that two OsCYP19-4 AS isoforms, OsCYP19-4.2 and OsCYP19-4.3, play roles linking auxin transport and cold stress via interactions with RCN1.
Areum Lee; Sang Sook Lee; Won Yong Jung; Hyun Ji Park; Bo Ra Lim; Hyun-Soon Kim; Jun Cheul Ahn; Hye Sun Cho. The OsCYP19-4 Gene Is Expressed as Multiple Alternatively Spliced Transcripts Encoding Isoforms with Distinct Cellular Localizations and PPIase Activities under Cold Stress. International Journal of Molecular Sciences 2016, 17, 1154 .
AMA StyleAreum Lee, Sang Sook Lee, Won Yong Jung, Hyun Ji Park, Bo Ra Lim, Hyun-Soon Kim, Jun Cheul Ahn, Hye Sun Cho. The OsCYP19-4 Gene Is Expressed as Multiple Alternatively Spliced Transcripts Encoding Isoforms with Distinct Cellular Localizations and PPIase Activities under Cold Stress. International Journal of Molecular Sciences. 2016; 17 (7):1154.
Chicago/Turabian StyleAreum Lee; Sang Sook Lee; Won Yong Jung; Hyun Ji Park; Bo Ra Lim; Hyun-Soon Kim; Jun Cheul Ahn; Hye Sun Cho. 2016. "The OsCYP19-4 Gene Is Expressed as Multiple Alternatively Spliced Transcripts Encoding Isoforms with Distinct Cellular Localizations and PPIase Activities under Cold Stress." International Journal of Molecular Sciences 17, no. 7: 1154.
Tissue-specific promoters for efficient expression of transgenes at specific times or in specific plant tissues can be applied to develop new transgenic plants. To exploit a promoter capable of driving strong expression in potato tubers, we isolated the promoter region of the laccase gene from potato (Solanum tuberosum L. cv. Desiree) and characterized its activity in transgenic Solanaceae plants, such as potato, tobacco and tomato. The ability of the laccase promoter to induce the β-glucuronidase (GUS) reporter was evaluated in independent transgenic potato lines and compared with that of the constitutive CaMV35S promoter. To determine the tissue specificity of expression in transgenic potato, GUS levels in shoot tips, leaves, stems, roots and tubers were measured by histochemical analysis. The laccase promoter conferred tuber-specific expression in transgenic potato regardless of the developmental stage, and there was no GUS reporter expression in leaves, stems or roots. Serial 5′ deletion analysis of the laccase promoter revealed that the tuber-specific regulatory elements might be scattered throughout the promoter. The laccase promoter responded weakly to salt stress, mannitol stress, and mechanical wounding but not to cold stress in the leaves and stems of transgenic potato. In transgenic tobacco, weak GUS expression driven by the laccase promoter was detected throughout the entire plant, whereas in transgenic tomato, GUS expression was detected only in the roots and seeds. Our data show that the laccase promoter represents a feasible candidate to drive high and preferential expression of genes in potato tubers.
Jang-Ho Ha; Ki-Beom Moon; Mi-Sun Kim; Se-Won Park; Kyu-Woong Hahn; Jae-Heung Jeon; Hyun-Soon Kim. The laccase promoter of potato confers strong tuber-specific expression in transgenic plants. Plant Cell, Tissue and Organ Culture (PCTOC) 2014, 120, 57 -68.
AMA StyleJang-Ho Ha, Ki-Beom Moon, Mi-Sun Kim, Se-Won Park, Kyu-Woong Hahn, Jae-Heung Jeon, Hyun-Soon Kim. The laccase promoter of potato confers strong tuber-specific expression in transgenic plants. Plant Cell, Tissue and Organ Culture (PCTOC). 2014; 120 (1):57-68.
Chicago/Turabian StyleJang-Ho Ha; Ki-Beom Moon; Mi-Sun Kim; Se-Won Park; Kyu-Woong Hahn; Jae-Heung Jeon; Hyun-Soon Kim. 2014. "The laccase promoter of potato confers strong tuber-specific expression in transgenic plants." Plant Cell, Tissue and Organ Culture (PCTOC) 120, no. 1: 57-68.
Erythropoietin (EPO) is a glycoprotein hormone that regulates red blood cell formation in mammals. It also plays an important role in wound healing and the brain’s response to neuronal injury. Recombinant EPO has been produced in various hosts including Escherichia coli, insect cells, and yeast. Plant systems are also useful for the production of recombinant proteins. In this study, we used Nicotiana benthamiana for the production of recombinant human EPO (rhEPO) using an Agrobacterium-mediated transient expression system. We evaluated the effect of repeated agroinfiltration and Agrobacterium density on the rhEPO production. In addition, the rhEPO expression vector was coinfiltrated with a vector expressing cucumber mosaic virus suppressor 2b (CMV2b), which suppresses posttranscriptional gene silencing. We found that rhEPO expression was increased approximately 5.5-fold in N. benthamiana leaves coinfiltrated with CMV2b. In contrast, neither Agrobacterium density nor the number of infiltrations influenced rhEPO expression.
Ki-Beom Moon; Jae-Heung Jeon; Woo-Sung Lee; HyunSoon Kim. Transient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana. Horticulture, Environment, and Biotechnology 2012, 53, 434 -440.
AMA StyleKi-Beom Moon, Jae-Heung Jeon, Woo-Sung Lee, HyunSoon Kim. Transient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana. Horticulture, Environment, and Biotechnology. 2012; 53 (5):434-440.
Chicago/Turabian StyleKi-Beom Moon; Jae-Heung Jeon; Woo-Sung Lee; HyunSoon Kim. 2012. "Transient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana." Horticulture, Environment, and Biotechnology 53, no. 5: 434-440.
Plastid transformation has to date been applied to the expression of heterologous genes involved in agronomic traits and to the production of useful recombinant proteins. Here, we report a feasibility study for producing the human β-site APP cleaving enzyme (BACE) via transformation of tobacco chloroplasts. Stable integration of human BACE into the plastome was confirmed by PCR. Genomic Southern blot analysis detected the presence of the tobacco aadA and human BACE genes between trnI and trnA in the plastome. Northern blot analysis revealed that the aadA and BACE genes were both properly transcribed into a dicistronic transcriptional unit. Human BACE protein expression in transplastomic tobacco was determined by western blot analysis. ELISA analysis revealed that, based on a dilution series of E. coli-derived BACE as a standard, transplastomic lines accumulated BACE to levels of 2.0% of total soluble proteins. When mice were gavaged with the transplastomic tobacco extracts, they showed an immune response against the BACE antigen. The successful production of plastid-based BACE protein has the potential for developing a plant-based vaccine against Alzheimer disease.
Jung Won Youm; Jae Heung Jeon; Hee Kim; Sung Ran Min; Mi Sun Kim; Hyouk Joung; Won Joong Jeong; Hyun Soon Kim. High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice. Transgenic Research 2010, 19, 1099 -1108.
AMA StyleJung Won Youm, Jae Heung Jeon, Hee Kim, Sung Ran Min, Mi Sun Kim, Hyouk Joung, Won Joong Jeong, Hyun Soon Kim. High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice. Transgenic Research. 2010; 19 (6):1099-1108.
Chicago/Turabian StyleJung Won Youm; Jae Heung Jeon; Hee Kim; Sung Ran Min; Mi Sun Kim; Hyouk Joung; Won Joong Jeong; Hyun Soon Kim. 2010. "High-level expression of a human β-site APP cleaving enzyme in transgenic tobacco chloroplasts and its immunogenicity in mice." Transgenic Research 19, no. 6: 1099-1108.
Ethylene-responsive factors (ERFs) are plant-specific transcription factors, many of which have been linked to plant defense responses. However, little is known about the functional significance of ERF genes in potato plants compared to the model plant species Arabidopsis. We show here that overexpression of CaPF1, an ERF/AP2-type pepper transcription factor gene, effectively increased tolerance to freezing, heat, heavy metal, and oxidative stress in potatoes. Interestingly, CaPF1 was involved in tuber formation in potato plants. The time course of microtuber formation was significantly retarded in potato plants that overexpressed CaPF1 compared with wild-type potato plants. Overall, the results of the present study indicate that the pepper transcription factor gene, CaPF1, is involved in promotion of multiple stress tolerance and retardation of in vitro tuberization in potato plants.
Jung Won Youm; Jae Heung Jeon; Doil Choi; So Young Yi; Hyouk Joung; Hyun Soon Kim. Ectopic expression of pepper CaPF1 in potato enhances multiple stresses tolerance and delays initiation of in vitro tuberization. Planta 2008, 228, 701 -708.
AMA StyleJung Won Youm, Jae Heung Jeon, Doil Choi, So Young Yi, Hyouk Joung, Hyun Soon Kim. Ectopic expression of pepper CaPF1 in potato enhances multiple stresses tolerance and delays initiation of in vitro tuberization. Planta. 2008; 228 (4):701-708.
Chicago/Turabian StyleJung Won Youm; Jae Heung Jeon; Doil Choi; So Young Yi; Hyouk Joung; Hyun Soon Kim. 2008. "Ectopic expression of pepper CaPF1 in potato enhances multiple stresses tolerance and delays initiation of in vitro tuberization." Planta 228, no. 4: 701-708.
Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer’s disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen.
Jung Won Youm; Jae Heung Jeon; Hee Kim; Young Ho Kim; Kisung Ko; Hyouk Joung; HyunSoon Kim. Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease. Biotechnology Letters 2008, 30, 1839 -1845.
AMA StyleJung Won Youm, Jae Heung Jeon, Hee Kim, Young Ho Kim, Kisung Ko, Hyouk Joung, HyunSoon Kim. Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease. Biotechnology Letters. 2008; 30 (10):1839-1845.
Chicago/Turabian StyleJung Won Youm; Jae Heung Jeon; Hee Kim; Young Ho Kim; Kisung Ko; Hyouk Joung; HyunSoon Kim. 2008. "Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease." Biotechnology Letters 30, no. 10: 1839-1845.
Patatins encoded by a multi-gene family are one of the major storage glycoproteins in potato tubers. Potato tubers have recently emerged as bioreactors for the production of human therapeutic glycoproteins (vaccines). Increasing the yield of recombinant proteins, targeting the produced proteins to specific cellular compartments, and diminishing expensive protein purification steps are important research goals in plant biotechnology. In the present study, potato patatins were eliminated almost completely via RNA interference (RNAi) technology to develop potato tubers as a more efficient protein expression system. The gene silencing effect of patatins in the transgenic potato plants was examined at individual isoform levels.
Yoon-Sik Kim; Yong-Hwa Lee; Hyun-Soon Kim; Mi-Sun Kim; Kyu-Woong Hahn; Jeong-Heon Ko; Hyouk Joung; Jae-Heung Jeon. Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins. BMC Biotechnology 2008, 8, 36 -36.
AMA StyleYoon-Sik Kim, Yong-Hwa Lee, Hyun-Soon Kim, Mi-Sun Kim, Kyu-Woong Hahn, Jeong-Heon Ko, Hyouk Joung, Jae-Heung Jeon. Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins. BMC Biotechnology. 2008; 8 (1):36-36.
Chicago/Turabian StyleYoon-Sik Kim; Yong-Hwa Lee; Hyun-Soon Kim; Mi-Sun Kim; Kyu-Woong Hahn; Jeong-Heon Ko; Hyouk Joung; Jae-Heung Jeon. 2008. "Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins." BMC Biotechnology 8, no. 1: 36-36.
The antibodies to preS2 synthetic peptides have been probed to neutralize hepatitis B virus (HBV), and also the addition of preS2 sequence could enhance the antibody response compared with a conventional vaccine in the non- and low responders. Previously, we generated transgenic potatoes expressing middle protein, which contains additional 55 amino acid preS2 region at the N-terminus of the S protein, of HBV to determine the feasibility of developing a plant-delivered HBV vaccine. In this study, we monitored the immune response after induction of immunoglobulin by boosting and assessed the efficacy of the mucosal immune response with regard to generate IgA antibodies. The HBsAg middle protein expressed in our transgenic potatoes was well immunized at low antigenic quantities in mice and the induced anti-S or anti-preS2 antibodies were sustained for the whole period without decrease. Orally delivery of plant-derived HBsAg middle protein to mice resulted in fecal anti-S or anti-preS2 as well as serum IgG. In addition, we used antibodies induced from the immunized mice with the potato-derived rHBsAg in competition assay as competitors to confirm the binding ability of preS2 antibodies to surface antigen of hepatitis virus. Anti-preS2 antibodies induced from immunized mice with transgenic potatoes effectively competed with anti-preS2 murine antibody H8 as expected. From these results, the inclusion of preS2 antigen to HBV plant vaccine may provide additional protective immunity in the HBV prevention.
Jung-Won Youm; Young-Suk Won; Jae Heung Jeon; Chun Jeih Ryu; Yang-Kyu Choi; Hyoung-Chin Kim; Byung-Dong Kim; Hyouk Joung; Hyun Soon Kim. Oral immunogenicity of potato-derived HBsAg middle protein in BALB/c mice. Vaccine 2007, 25, 577 -584.
AMA StyleJung-Won Youm, Young-Suk Won, Jae Heung Jeon, Chun Jeih Ryu, Yang-Kyu Choi, Hyoung-Chin Kim, Byung-Dong Kim, Hyouk Joung, Hyun Soon Kim. Oral immunogenicity of potato-derived HBsAg middle protein in BALB/c mice. Vaccine. 2007; 25 (3):577-584.
Chicago/Turabian StyleJung-Won Youm; Young-Suk Won; Jae Heung Jeon; Chun Jeih Ryu; Yang-Kyu Choi; Hyoung-Chin Kim; Byung-Dong Kim; Hyouk Joung; Hyun Soon Kim. 2007. "Oral immunogenicity of potato-derived HBsAg middle protein in BALB/c mice." Vaccine 25, no. 3: 577-584.