This study aimed to evaluate in vitro and in vivo dietary supplementation with different levels of natural or nano-zeolite forms on rumen fermentation patterns and nutrient digestibility. In the in vitro experiment, a basal diet (50% concentrate: 50% forage) was incubated without additives (control) and with natural zeolite (10, 20, 30 g/kg DM) or nano-zeolite (0.2, 0.3, 0.4, 0.5, 1.0 g/kg DM) for 24 h to assess their effect on ruminal fermentation, feed degradability, and gas and methane production using a semi-automatic system of in vitro gas production (GP). The most effective doses obtained from the in vitro experiment were evaluated in vivo using 30 Barki goats (26 ± 0.9 SE kg body weight). Goats were allocated into three dietary treatments (n = 10/treatment) as follows: control (basal diet without any supplementations), natural zeolite (20 g/kg DM diet), and nano-zeolite (0.40 g/kg DM diet). The in vitro results revealed that only the nano-zeolite supplementation form quadratically (p= 0.004) increased GP, and the level of 0.5 g/kg DM had the highest GP value compared to the control. Both zeolite forms affected the CH₄ production, linear, and quadratic reductions (p < 0.05) in CH₄ (mL/g DM), consistent with linear increases in truly degraded organic matter (TDOM) (p = 0.09), and propionate molar proportions (p = 0.007) were observed by nano zeolite treatment, while the natural form of zeolite resulted in a linear CH₄ reduction consistent with a linear decrease (p = 0.004) in NH₃-N, linear increases in TDOM (p = 0.09), and propionate molar proportions (p = 0.004). Results of the in vivo experiment demonstrated that the nutrient digestibility was similar among all treatments. Nano zeolite enhanced (p < 0.05) the total short-chain fatty acids and butyrate concentrations, while both zeolite forms decreased (p < 0.001) NH₃-N compared to the control. These results suggested that both zeolite supplementation forms favorably modified the rumen fermentation in different patterns.

This study was conducted to assess the impacts of using two essential oils, rosemary and ginger, on growing rabbits’ performance, carcass traits, meat composition, blood biochemicals, and the redox status of growing New Zealand White (NZW) rabbits. A total of 120 unsexed NZW rabbits, 42-days-old, were assigned randomly to five experimental groups (n = 24, 6 replicates with 4 rabbits each). The first group received a basal diet (control), the second to fifth groups were dietary supplemented daily with rosemary essential oil (REO) and ginger essential oil (GEO) at doses of 0.25 and 0.5% for each supplementation (REO-0.25, REO-0.5, GEO-0.25, and GEO-0.5), respectively. The growth traits were studied for 7 weeks, from the 7th to the 13th week of the rabbits’ age. The results revealed that final body weight, weight gain, and average daily gain increased significantly (p < 0.01) in the REO-0.5 and GEO-0.5 treatments compared to the control group. Daily feed intake decreased (p = 0.005) in essential oil treatments. Meanwhile, the feed conversion ratio improved significantly (p = 0.001) in REO and GEO at the high doses compared to the control group. The weight percentages of liver and giblets increased (p < 0.001) with both treatments of REO and GEO compared to the control group. The dietary supplementation with REO and GEO did not affect (p > 0.05) the meat composition of Longissimus dorsi and hind leg muscles. Meanwhile, REO and GEO supplementation significantly decreased cholesterol levels in the rabbit meat. Thiobarbituric acid reactive substance concentrations decreased by 10 and 15% in the meat of REO-0.5 and GEO-0.5 treatments, respectively, compared to the other groups. In the same trend, REO and GEO treatments induced a significant (p = 0.001) reduction in the plasma cholesterol concentrations and triglycerides compared to the control. The total antioxidant capacity increased by 7.60% and the malondialdehyde decreased by 11.64% in the plasma of GEO-0.5 treatment than the control. Thus, the dietary supplementation of REO and GEO have a beneficial effect in improving the productivity and meat quality of growing rabbits.

Two types of modified nano-montmorillonite (MNM) were developed by ion-exchange reactions using two different surfactants; sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CETAB), to prepare MNM_SDS and MNM_CETAB, respectively. Both MNM types were on the nano-scale and had higher cation-exchange capacity values than NM clay. The MNM_CETAB had the highest zeta potential (−27 mV) compared with the other clays. Effects of MNM types on in vitro ruminal batch culture fermentation, nutrient degradability, and methane (CH₄) emission compared with monensin were evaluated in vitro using a semi-automatic gas production system. The experimental treatments were the control (0 supplementations), monensin (40 mg/kg DM), and NM (5 g NM/kg DM), and two levels of MNM_SDS and MNM_CETAB were supplemented at 0.05 (low) and 0.5 (high) g/kg DM to the control basal feed substrate. Among the experimental treatments, the high dose of both MNM types reduced (p < 0.01) CH₄ production and ammonia concentrations compared with the control, while only MNM_CETAB treatment tended to increase (p = 0.08) the truly degraded organic matter compared with monensin. All MNM treatments increased (p < 0.01) acetate molar proportions compared with monensin. The high MNM_CETAB increased (p < 0.01) the in vitro ruminal batch culture pH compared with the control and monensin. The MNM_CETAB supplemented at 0.5 g/kg DM is the most efficient additive to reduce CH₄ emission with the advantage of enhancing the in vitro nutrient degradability of the experimental feed substrate. These results indicated that MNM could modulate the in vitro ruminal fermentation pattern in a dose- and type-dependent manner.

Balancing maximum biomass yield with high nutritional quality and reduced environmental impact remains a primary challenge in sustainable summer forage production for livestock, a challenge intensified by competition between conventional and forage crops for agricultural land, particularly regarding planting dates and land occupation duration, which limits available livestock forage resources. To address this issue, this study evaluated the forage yield, quality, and nutritive value of teosinte (Zea Mexicana L.) under three sowing dates: 1 May, 20 May, and 10 June, and three harvest ages: 40, 55, and 70 days after sowing (DAS), under Egyptian field conditions. Results showed that delaying harvesting until 70 DAS produced the highest fresh (34.95 to 46.0 t ha⁻¹) and dry (5.38 to 6.03 t ha⁻¹) yields for the late sowing date (10 June). The in vitro gas production (GP) assay showed that GP was significantly higher for teosinte sown on May 20 and June 10 but declined progressively with advancing harvest age. The NH₃-N concentration decreased (p < 0.05) when teosinte was sown on May 1 and harvested at 40 DAS. True organic matter degradability declined for teosinte sown on May 1, May 20, and June 10 when harvested at 70 DAS, whereas it increased at harvest ages of 40 and 55 DAS for teosinte sown on May 1 and June 10. Teosinte sown on May 1 produced significantly lower methane emissions than that sown on May 20 or June 10, regardless of harvest age (40, 55, or 70 DAS). Overall, delaying harvest until 70 DAS resulted in the highest fresh and dry matter yields but significantly decreased gas production and organic matter degradability compared to earlier harvests, which require future research to optimize the utilization of teosinte.