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Algae are an underexploited source of natural bioactive compounds in Western countries, so an increasing interest in the valorization of these marine organisms has emerged in recent years. In this work, the effect of extracting solvent on the extraction yield, phenolic content, antioxidant capacity, and antimicrobial activity of nine brown macroalgae species (Ascophyllum nodosum, Himanthalia elongata, Undaria pinnatifida, Pelvetia canaliculata, Saccharina latissima, Bifurcaria bifurcata, Laminaria ochroleuca, Sargassum muticum, and Fucus spiralis) was assessed. Total phenolic content (TPC) and the antioxidant properties of extracts by different assays: radical scavenging activity (DPPH-RSA) and ferric reducing antioxidant power (FRAP) were performed. The antimicrobial activity of extracts was studied against six different foodborne microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, Salmonella enteritidis, and Pseudomonas aeruginosa. The highest extraction yield was achieved in ethanolic extracts. However, the highest TPC and FRAP values were obtained on the ethyl acetate extracts, especially from A. nodosum. Concerning algal species, the highest TPC and FRAP values were found in A. nodosum, while the highest DPPH-RSA values were achieved in the hexane extracts of B. bifurcata. The antimicrobial activity of algal extracts varied according to the solvent and alga selected, suggesting the species- and solvent-dependent behavior of this property, with B. bifurcata extracts showing the highest results for a wide range of bacteria. Our results provide insight on the characterization of widespread brown algae in the coasts of the North-Western region of the Iberian Peninsula, reflecting multiple health-enhancing properties which may lead to their exploitation in food, pharmacological, and cosmetic industries.
Aurora Silva; Carla Rodrigues; Paula Garcia-Oliveira; Catarina Lourenço-Lopes; Sofia A. Silva; Pascual Garcia-Perez; Ana P. Carvalho; Valentina F. Domingues; M. Fátima Barroso; Cristina Delerue-Matos; Jesus Simal-Gandara; Miguel A. Prieto. Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula. Foods 2021, 10, 1915 .
AMA StyleAurora Silva, Carla Rodrigues, Paula Garcia-Oliveira, Catarina Lourenço-Lopes, Sofia A. Silva, Pascual Garcia-Perez, Ana P. Carvalho, Valentina F. Domingues, M. Fátima Barroso, Cristina Delerue-Matos, Jesus Simal-Gandara, Miguel A. Prieto. Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula. Foods. 2021; 10 (8):1915.
Chicago/Turabian StyleAurora Silva; Carla Rodrigues; Paula Garcia-Oliveira; Catarina Lourenço-Lopes; Sofia A. Silva; Pascual Garcia-Perez; Ana P. Carvalho; Valentina F. Domingues; M. Fátima Barroso; Cristina Delerue-Matos; Jesus Simal-Gandara; Miguel A. Prieto. 2021. "Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula." Foods 10, no. 8: 1915.
The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.
Cristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods 2021, 10, 1366 .
AMA StyleCristina Soares, Sara Sousa, Susana Machado, Elsa Vieira, Ana Carvalho, Maria Ramalhosa, Simone Morais, Manuela Correia, Teresa Oliva-Teles, Valentina Domingues, Cristina Delerue-Matos. Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination. Foods. 2021; 10 (6):1366.
Chicago/Turabian StyleCristina Soares; Sara Sousa; Susana Machado; Elsa Vieira; Ana Carvalho; Maria Ramalhosa; Simone Morais; Manuela Correia; Teresa Oliva-Teles; Valentina Domingues; Cristina Delerue-Matos. 2021. "Bioactive Lipids of Seaweeds from the Portuguese North Coast: Health Benefits versus Potential Contamination." Foods 10, no. 6: 1366.
A multiresidue analytical methodology based on a solid-phase microextraction (SPME), followed by gas chromatography-ion trap mass spectrometry (GC–IT/MS), has been developed for trace analysis of 20 known or suspected endocrine-disrupting pesticides. The SPME conditions are optimized considering several key parameters to obtain the maximum sensitivity. After the optimization, the method validation is performed, and the limits of detection (ranged from 2–150 ng/L) and the coefficient of determination (above 0.990) of studied compounds are determined for all the analytes. A robust sampling of twenty sampling points of surface water samples from the north and center of Portugal is performed, and the validated methodology is applied. In total, 20 compounds from four chemical families (13 organochlorine, 1 organophosphorus, 2 dicarboximide, and 4 pyrethroids) are studied, and the pesticides most frequently detected are eight organochlorine pesticides (α-, β-HCHs, lindane, HCB, o,p′-DDT, p,p′-DDE, p,p′-DDD, α-endosulfan), cypermethrin, and vinclozolin.
José Vera; Virgínia Fernandes; Luísa Correia-Sá; Catarina Mansilha; Cristina Delerue-Matos; Valentina Domingues. Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS. Separations 2021, 8, 81 .
AMA StyleJosé Vera, Virgínia Fernandes, Luísa Correia-Sá, Catarina Mansilha, Cristina Delerue-Matos, Valentina Domingues. Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS. Separations. 2021; 8 (6):81.
Chicago/Turabian StyleJosé Vera; Virgínia Fernandes; Luísa Correia-Sá; Catarina Mansilha; Cristina Delerue-Matos; Valentina Domingues. 2021. "Occurrence of Selected Known or Suspected Endocrine-Disrupting Pesticides in Portuguese Surface Waters Using SPME-GC-IT/MS." Separations 8, no. 6: 81.
The importance of food quality has become a crucial issue due to the widespread use of pesticides in agriculture. The frequent use of pesticides has resulted in contamination of the environmental, fruit crops and also caused potential risk to human health. Strawberries are at the top of the list of fruits with the highest concentration of pesticides. To date, several analytical techniques have been used for the extraction and detection of pesticides in strawberries, from classic to advanced analysis methodologies. In this chapter, the studies published by the scientific community performed from 2000 to the present, concerning the extraction and analysis of pesticide residues in strawberries, were discussed. The most broadly used detection technique for the analyse of pesticides in strawberries is mass spectrometry combined with gas and/or liquid chromatography. In general, multi-residue methods with selective sample treatment methodologies have been developed for this purpose. Quick, Easy, Cheap, Effective, Rugged, Safe (QuEChERS), liquid-liquid, solid-liquid, and solid-phase extraction and microextractions are the selected sample preparation techniques. The analytical parameters of the methodology and the findings on pesticide residues are described. The presented chapter shows the importance of these data in terms of food safety on the situation regarding pesticide residue in strawberries and human health regarding the risk of the ingestion of pesticides contaminated strawberries.
Virgínia Cruz Fernandes; M. Luz Maia; Luísa Correia Sá; Sara Sousa; Paula Paíga; José L. Vera; Valentina F. Domingues; Cristina Delerue-Matos. Extraction Procedures and Chromatography of Pesticides Residues in Strawberries. Sustainable Agriculture Reviews 2020, 167 -201.
AMA StyleVirgínia Cruz Fernandes, M. Luz Maia, Luísa Correia Sá, Sara Sousa, Paula Paíga, José L. Vera, Valentina F. Domingues, Cristina Delerue-Matos. Extraction Procedures and Chromatography of Pesticides Residues in Strawberries. Sustainable Agriculture Reviews. 2020; ():167-201.
Chicago/Turabian StyleVirgínia Cruz Fernandes; M. Luz Maia; Luísa Correia Sá; Sara Sousa; Paula Paíga; José L. Vera; Valentina F. Domingues; Cristina Delerue-Matos. 2020. "Extraction Procedures and Chromatography of Pesticides Residues in Strawberries." Sustainable Agriculture Reviews , no. : 167-201.
Pyrethroids are a class of extensively used insecticides for human and animal health protection. Concern has been growing about the possible human health effects from environmental exposure, e.g. chronic exposure to low levels, to pyrethroids. Several metabolites, specific or common to different pyrethroids, can be used for the assessment of pyrethroids exposure. 3-Phenoxybenzoic acid is a major urinary biomarker for numerous pyrethroids used nowadays and has been extensively analyzed and reported. Biomonitoring pyrethroids in human samples is undoubtedly necessary in order to support the clarification of potential correlations between exposure and adverse health effects. The present chapter is an overview of pyrethroids’ metabolitesanalyses in human urine samples, including analytical methods and reported values. Data from several studies from 1990 until March 2019 were collected. Studies from different countries and continents that assess exposure in diverse populations are addressed and different applied analytical methodologies are also discussed. Additionally, human biomonitoring was performed based on highly sensitive analytical methods and reports values for several metabolites, showing the relevance of human biomonitoring of pyrethroids. Some metabolites were below the human biomonitoring assessment values of 2 μg/L for the general population. However, we must consider that exceeds in the upper exposure percentiles of the general population, e.g. children.
Luísa Correia Sá; Sara Sousa; Virgínia Cruz Fernandes; M. Luz Maia; Cristina Delerue-Matos; Valentina F. Domingues. Pyrethroids Metabolites in Human Urine Samples. Sustainable Agriculture Reviews 2020, 227 -270.
AMA StyleLuísa Correia Sá, Sara Sousa, Virgínia Cruz Fernandes, M. Luz Maia, Cristina Delerue-Matos, Valentina F. Domingues. Pyrethroids Metabolites in Human Urine Samples. Sustainable Agriculture Reviews. 2020; ():227-270.
Chicago/Turabian StyleLuísa Correia Sá; Sara Sousa; Virgínia Cruz Fernandes; M. Luz Maia; Cristina Delerue-Matos; Valentina F. Domingues. 2020. "Pyrethroids Metabolites in Human Urine Samples." Sustainable Agriculture Reviews , no. : 227-270.
The search for food resources is a constant in human history. Nowadays, the search for natural and safe food supplies is of foremost importance. Accordingly, there is a renewed interest in eco-friendly and natural products for substitution of synthetic additives. In addition, microbial contamination of food products during their obtaining and distribution processes is still a sanitary issue, and an important target for the food industry is to avoid food contamination and its related foodborne illnesses. These diseases are fundamentally caused by certain microorganisms listed in this review and classified according to their Gram negative or positive character. Algae have proven to possess high nutritional value and a wide variety of biological properties due to their content in active compounds. Among these capabilities, macroalgae are recognized for having antimicrobial properties. Thus, the present paper revises the actual knowledge of microbial contaminants in the food industry and proposes antimicrobial algal compounds against those pathogenic bacteria responsible for food contamination as valuable molecules for its growth inhibition. The capacity of algae extracts to inhibit some major food pathogen growth was assessed. Moreover, the main applications of these compounds in the food industry were discussed while considering their favorable effects in terms of food safety and quality control.
Aurora Silva; Sofia A. Silva; C. Lourenço-Lopes; C. Jimenez-Lopez; M. Carpena; P. Gullón; M. Fraga-Corral; V. F. Domingues; M. Fátima Barroso; J. Simal-Gandara; M. A. Prieto. Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens. Antibiotics 2020, 9, 712 .
AMA StyleAurora Silva, Sofia A. Silva, C. Lourenço-Lopes, C. Jimenez-Lopez, M. Carpena, P. Gullón, M. Fraga-Corral, V. F. Domingues, M. Fátima Barroso, J. Simal-Gandara, M. A. Prieto. Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens. Antibiotics. 2020; 9 (10):712.
Chicago/Turabian StyleAurora Silva; Sofia A. Silva; C. Lourenço-Lopes; C. Jimenez-Lopez; M. Carpena; P. Gullón; M. Fraga-Corral; V. F. Domingues; M. Fátima Barroso; J. Simal-Gandara; M. A. Prieto. 2020. "Antibacterial Use of Macroalgae Compounds against Foodborne Pathogens." Antibiotics 9, no. 10: 712.
In the last few decades, attention on new natural antimicrobial compounds has arisen due to a change in consumer preferences and the increase in the number of resistant microorganisms. Macroalgae play a special role in the pursuit of new active molecules as they have been traditionally consumed and are known for their chemical and nutritional composition and their biological properties, including antimicrobial activity. Among the bioactive molecules of algae, proteins and peptides, polysaccharides, polyphenols, polyunsaturated fatty acids and pigments can be highlighted. However, for the complete obtaining and incorporation of these molecules, it is essential to achieve easy, profitable and sustainable recovery of these compounds. For this purpose, novel liquid–liquid and solid–liquid extraction techniques have been studied, such as supercritical, ultrasound, microwave, enzymatic, high pressure, accelerated solvent and intensity pulsed electric fields extraction techniques. Moreover, different applications have been proposed for these compounds, such as preservatives in the food or cosmetic industries, as antibiotics in the pharmaceutical industry, as antibiofilm, antifouling, coating in active packaging, prebiotics or in nanoparticles. This review presents the main antimicrobial potential of macroalgae, their specific bioactive compounds and novel green extraction technologies to efficiently extract them, with emphasis on the antibacterial and antifungal data and their applications.
Aurora Silva; Sofia A. Silva; M. Carpena; P. Garcia-Oliveira; P. Gullón; M. Fátima Barroso; M.A. Prieto; J. Simal-Gandara. Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications. Antibiotics 2020, 9, 642 .
AMA StyleAurora Silva, Sofia A. Silva, M. Carpena, P. Garcia-Oliveira, P. Gullón, M. Fátima Barroso, M.A. Prieto, J. Simal-Gandara. Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications. Antibiotics. 2020; 9 (10):642.
Chicago/Turabian StyleAurora Silva; Sofia A. Silva; M. Carpena; P. Garcia-Oliveira; P. Gullón; M. Fátima Barroso; M.A. Prieto; J. Simal-Gandara. 2020. "Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications." Antibiotics 9, no. 10: 642.
Synthetic musks and organophosphorus pesticides represent a potential risk to the human health since exposure can lead to distinct types of carcinogenesis and endocrine disorders. These are lipophilic compounds as such, prone to deposit and persist in fat tissues, mainly in adipose tissue. Very few studies have reported on the occurrence and accumulation profile of these contaminants in human adipose tissue. Analytical methods for the detection and quantification of synthetic musks and organophosphorus pesticides in adipose tissue are lacking. In this study, the efficacy of different extraction with ultrasonic homogenizer and dispersive solid-phase extraction (d-SPE) clean-up methods were evaluated in human adipose tissue. The relative sample clean-up was assessed by measurement of total lipid content. The quantification of four synthetic musks and six organophosphorus pesticides were performed by gas chromatography (GC) mass spectrometry (MS) and flame photometric detection (FPD), respectively. The d-SPE clean-up with 50 mg PSA, 150 mg MgSO4, 100 mg C18EC and 50 mg Z-Sep provided the most effective clean-up, removing the greatest amount of interfering substances including lipids and simultaneously ensuring good chromatographic separation and recoveries. Method detection limits were between 4 to 9 ng/g for synthetic musk and 1 to 7 ng/g for organophosphorus pesticides in adipose tissue. The proposed method was applied to adipose tissue of obese patients and positive samples were confirmed with GC tandem mass spectrometry. Galaxolide was found in all the samples tested with concentrations ranging from 0.08 to 0.5 μg/g of adipose tissue. No other synthetic musk studied was detected. Organophosphorus pesticides were not found in the analysed samples. The developed analytical procedures were successful and can easily be applied to biomonitoring these compounds in human adipose tissue.
Sara Sousa; Diogo Pestana; Gil Faria; Fernando Vasconcelos; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue. Journal of Pharmaceutical and Biomedical Analysis 2020, 191, 113598 .
AMA StyleSara Sousa, Diogo Pestana, Gil Faria, Fernando Vasconcelos, Cristina Delerue-Matos, Conceição Calhau, Valentina Fernandes Domingues. Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue. Journal of Pharmaceutical and Biomedical Analysis. 2020; 191 ():113598.
Chicago/Turabian StyleSara Sousa; Diogo Pestana; Gil Faria; Fernando Vasconcelos; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. 2020. "Method development for the determination of Synthetic Musks and Organophosphorus Pesticides in Human Adipose Tissue." Journal of Pharmaceutical and Biomedical Analysis 191, no. : 113598.
Shrimps are widely distributed in coastal areas, estuaries and rivers. Although this shellfish is a good source of nutrients, it can also accumulate environmental contaminants, such as organochlorine pesticides (OCPs), brominated flame retardants (BFRs), synthetic musks (SMs) and polycyclic aromatic hydrocarbons (PAHs). Due to their bioaccumulative properties, these pollutants are endocrine disruptors. In this review, an overview of the world's shrimp market, pollutants legislation and values found in shrimp samples will be discussed. Shrimps analysed from all continents showed the presence of contaminants, Asia being the continent with the highest values reported. The concentration values reached a maximum of 26100 ng/g wet weight (ww) for OCPs, of 226.45 ng/g ww for BFRs, of 12.1 ng/g ww for SMs and of 50650 ng/g ww for PAHs. Exposure data and risk, taken from different studies, are very variable and indicate that shrimp's consumption may represent a risk especially in certain geographic areas.
Maria Luz Maia; Sara Sousa; Luísa Correia-Sá; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure. Heliyon 2020, 6, 1 .
AMA StyleMaria Luz Maia, Sara Sousa, Luísa Correia-Sá, Cristina Delerue-Matos, Conceição Calhau, Valentina Fernandes Domingues. Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure. Heliyon. 2020; 6 (9):1.
Chicago/Turabian StyleMaria Luz Maia; Sara Sousa; Luísa Correia-Sá; Cristina Delerue-Matos; Conceição Calhau; Valentina Fernandes Domingues. 2020. "Organochlorine pesticides, brominated flame retardants, synthetic musks and polycyclic aromatic hydrocarbons in shrimps. An overview of occurrence and its implication on human exposure." Heliyon 6, no. 9: 1.
This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a concentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD < 5.2 % was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean.
Stephanie L. Morais; Piedade Barros; Marlene Santos; Cristina Delerue-Matos; Andreia C. Gomes; M. Fátima Barroso. Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta 2020, 222, 121416 .
AMA StyleStephanie L. Morais, Piedade Barros, Marlene Santos, Cristina Delerue-Matos, Andreia C. Gomes, M. Fátima Barroso. Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta. 2020; 222 ():121416.
Chicago/Turabian StyleStephanie L. Morais; Piedade Barros; Marlene Santos; Cristina Delerue-Matos; Andreia C. Gomes; M. Fátima Barroso. 2020. "Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates." Talanta 222, no. : 121416.
The effect of carp feeding with n-3 PUFA-enriched feed (Schizochytrium sp. meal or salmon oil) on nutritional quality indicators (proximal composition, fatty acid profile of fat) and culinary quality (color parameters, texture, sensory properties) was evaluated. Highly significant effects of carp nutrition on chemical composition and fat characteristics, L* and a* color parameters, muscle fiber size, endomysium thickness, moisture and taste of fillets were determined. Fillets obtained from carps fed with the experimental feed contained less protein and more crude fat and had larger muscle fibers, but scored more highly in the sensory evaluation of moisture and fishy taste. In the fat of carp fed the enriched feed, a greater share of total PUFA, n-3 PUFA, total EPA and DHA, n-3/n-6 ratio, and a smaller share of total MUFA were observed compared with control fish. However, no effect of nutrition on the texture of carp fillets, assessed either instrumentally or using sensory methods, was found. The use of Schizochytrium sp. meal as a source of EPA and DHA gave much better results than salmon oil, as it allowed a higher content of these valuable fatty acids to be achieved, without compromising quality.
M. Sobczak; Remigiusz Panicz; P. Eljasik; J. Sadowski; A. Tórz; J. Żochowska-Kujawska; Vera Liane Barbosa; Valentina Domingues; António Marques; Jorge Dias. Quality improvement of common carp (Cyprinus carpio L.) meat fortified with n-3 PUFA. Food and Chemical Toxicology 2020, 139, 111261 .
AMA StyleM. Sobczak, Remigiusz Panicz, P. Eljasik, J. Sadowski, A. Tórz, J. Żochowska-Kujawska, Vera Liane Barbosa, Valentina Domingues, António Marques, Jorge Dias. Quality improvement of common carp (Cyprinus carpio L.) meat fortified with n-3 PUFA. Food and Chemical Toxicology. 2020; 139 ():111261.
Chicago/Turabian StyleM. Sobczak; Remigiusz Panicz; P. Eljasik; J. Sadowski; A. Tórz; J. Żochowska-Kujawska; Vera Liane Barbosa; Valentina Domingues; António Marques; Jorge Dias. 2020. "Quality improvement of common carp (Cyprinus carpio L.) meat fortified with n-3 PUFA." Food and Chemical Toxicology 139, no. : 111261.
This study aimed to develop new canned chub mackerel products incorporating edible seaweeds (Ascophyllum nodosum, Fucus spiralis, Saccorhiza polyschides, Chondrus crispus, Porphyra sp. and Ulva sp.) harvested in the Portuguese North-Central coast, with simultaneous sensory improvement and minerals enrichment. Two processes were compared, namely the addition of seaweeds in i) the canning step and ii) in the brining step (as the replacement for salt). The concentrations of four macrominerals (Na, K, Ca and Mg), chloride, and twelve trace elements (Co, Cu, Fe, I, Li, Mn, Mo, Rb, Se, Sr, V and Zn) were determined by high-resolution continuum source flame atomic absorption spectrometry (HR-CS-FAAS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. Results showed that canned chub mackerel incorporating C. crispus and F. spiralis was found to be the preferred sensory option, also exhibiting contents enriched with Cl, Co, Cu, Fe, I, Li, Mg, Mn, Mo, Na, Rb, Se, and Sr. This effect was more pronounced when both seaweed species were added to replace the salt added in the brining step.
Elsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules 2020, 25, 1133 .
AMA StyleElsa F. Vieira, Cristina Soares, Susana Machado, M. Teresa Oliva-Teles, Manuela Correia, Maria João Ramalhosa, Ana Carvalho, Valentina F. Domingues, Filipa Antunes, Simone Morais, Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules. 2020; 25 (5):1133.
Chicago/Turabian StyleElsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. 2020. "Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition." Molecules 25, no. 5: 1133.
One of the main challenges for analytical laboratories and food safety authorities is the control of food contaminants hazardous to human health. For the first time, a simple, fast and cost-effective sample preparation method is proposed as an extraction technique to determine 12 brominated flame retardants (BFRs) (7 congeners polybrominated diphenyl ether (PBDE) and 5 novel BFRs) in Capsicum cultivars. Different QuEChERS and dispersive solid-phase extract (d-SPE) sorbent compositions were evaluated in terms of recovery and matrix effects. The best results were obtained with citrate buffered version QuEChERS and a cleanup step, with 150 mg MgSO4, 50 mg PSA, 50 mg C18, and 5 mg of carbon. The limit of detection (LOD) was between 1.4-9.3 µg/kg and R2>0.99. Recoveries and matrix effects were between 66-104% and 0.58-2.18, respectively. The relative standard deviations from repeatability and reproducibility studies and estimation of measurement uncertainty were lower than 20%. Gas chromatography coupled with a mass spectrometer was used to confirm the presence of the BFRs in the samples. Novel BFRs were detected lower than the LOD.
Virgínia Cruz Fernandes; Ward Luts; Cristina Delerue-Matos; Valentina Fernandes Domingues. Improved QuEChERS for Analysis of Polybrominated Diphenyl Ethers and Novel Brominated Flame Retardants in Capsicum Cultivars Using Gas Chromatography. Journal of Agricultural and Food Chemistry 2020, 68, 3260 -3266.
AMA StyleVirgínia Cruz Fernandes, Ward Luts, Cristina Delerue-Matos, Valentina Fernandes Domingues. Improved QuEChERS for Analysis of Polybrominated Diphenyl Ethers and Novel Brominated Flame Retardants in Capsicum Cultivars Using Gas Chromatography. Journal of Agricultural and Food Chemistry. 2020; 68 (10):3260-3266.
Chicago/Turabian StyleVirgínia Cruz Fernandes; Ward Luts; Cristina Delerue-Matos; Valentina Fernandes Domingues. 2020. "Improved QuEChERS for Analysis of Polybrominated Diphenyl Ethers and Novel Brominated Flame Retardants in Capsicum Cultivars Using Gas Chromatography." Journal of Agricultural and Food Chemistry 68, no. 10: 3260-3266.
Olga Freitas; Maria João Ramalhosa; Cristina Soares; Abel Duarte; M. Fátima Barroso; Rita Alves; A.P. Carvalho; João Grosso; Lúcia Santos; Manuela M. Moreira; Cristina Delerure-Matos. ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION. ICERI2019 Proceedings 2019, 10636 -10643.
AMA StyleOlga Freitas, Maria João Ramalhosa, Cristina Soares, Abel Duarte, M. Fátima Barroso, Rita Alves, A.P. Carvalho, João Grosso, Lúcia Santos, Manuela M. Moreira, Cristina Delerure-Matos. ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION. ICERI2019 Proceedings. 2019; ():10636-10643.
Chicago/Turabian StyleOlga Freitas; Maria João Ramalhosa; Cristina Soares; Abel Duarte; M. Fátima Barroso; Rita Alves; A.P. Carvalho; João Grosso; Lúcia Santos; Manuela M. Moreira; Cristina Delerure-Matos. 2019. "ARTISTS CONNECTION BETWEEN RECYCLING AND ENVIRONMENTAL EDUCATION." ICERI2019 Proceedings , no. : 10636-10643.
A sample preparation method, QuEChERS extraction combined with a magnetic micro dispersive solid phase extraction (MµdSPE), was optimized and evaluated for the trace analysis of 9 brominated flame retardants in red fruit samples (strawberries, blueberries, and raspberries) using gas chromatography-mass spectrometry. Magnetic nanomaterials were used as sorbents providing an extraction of the target compounds. Linearity was established for all the analytes (from 10 to 200 µg kg−1). Seven concentration levels were analyzed with three measurements at each concentration. Linear responses (R2 > 0.99) were obtained, recoveries of all target analytes were within the range of 65–141%, relative standard deviations were <20% at all three spiking levels, while intraday and interday precisions were below 20%. This study demonstrated that the new sample preparation with magnetic nanoparticles could potentially be expanded to extract and pre-concentrate the BFRs in different red fruit samples. The method has been successfully applied to study BFRs in 12 samples from conventional and organic farming.
Virgínia Cruz Fernandes; Maria Castro Freitas; João G. Pacheco; Valentina Domingues; Cristina Delerue-Matos. Evaluation of the QuEChERS and magnetic micro dispersive solid-phase extraction of brominated flame retardants in red fruits with determination by GC/MS. Food Chemistry 2019, 309, 125572 .
AMA StyleVirgínia Cruz Fernandes, Maria Castro Freitas, João G. Pacheco, Valentina Domingues, Cristina Delerue-Matos. Evaluation of the QuEChERS and magnetic micro dispersive solid-phase extraction of brominated flame retardants in red fruits with determination by GC/MS. Food Chemistry. 2019; 309 ():125572.
Chicago/Turabian StyleVirgínia Cruz Fernandes; Maria Castro Freitas; João G. Pacheco; Valentina Domingues; Cristina Delerue-Matos. 2019. "Evaluation of the QuEChERS and magnetic micro dispersive solid-phase extraction of brominated flame retardants in red fruits with determination by GC/MS." Food Chemistry 309, no. : 125572.
Vegetable oils (VO) are generally accepted as valuable alternatives to fish oils but are devoid of n-3 LC-PUFA (Long-Chain-Polyunsaturated Fatty Acids) and rich in C18-PUFA. Marine fish have limited capacity to express Δ6/Δ5 desaturases (FADS1 and FADS2) and elongases of very-long-chain fatty acids (Elovl), which severely limits their capacity to produce LC-PUFA from C18-PUFA. This study aimed to evaluate the effects of dietary essential fatty acids(EFA) ratios, arachidonic (ARA), eicosapentaenoic (EPA), and docosahexaenoic (DHA) acids on growth performance, feed utilization, whole-body composition, hepatic and intestinal activity of key-enzymes of lipid and carbohydrate metabolism, and expression of lipid metabolism-related genes. Diets were formulated to include ARA/EPA/DHA ratios of 2.0/0.2/0.1 (Diet A); 1.0/0.4/0.4 (Diet B); 0/0.6/0.6 (Diet C); and 0/0.3/1.5 (Diet D) and were fed to triplicate groups of gilthead sea bream juveniles for 56 days. Dietary EFA ratios did not affect growth performance and whole-body composition. Nevertheless, feed and protein utilization were higher with diet B than diets C or D. Except for ARA, muscle EFA profile followed that of the diets. Plasma triglycerides were higher with diet B and C than A, and plasma cholesterol decreased in fish fed the higher DHA diet. Hepatic ELOVL5 and phospholipase A2 were upregulated in fish fed diet C compared to diet A. In the intestine, ELOVL5 levels were down-regulated in fish fed diet A and D compared to fish fed diet B. Overall, results showed that besides EPA and DHA, adequate dietary ARA level needs to be provided to optimize feed utilization efficiency of gilthead sea bream juveniles.
R. Magalhães; Inês Guerreiro; Filipe Coutinho; Sara Moutinho; Sara Sousa; C. Delerue-Matos; Valentina Domingues; R.E. Olsen; Helena Peres; Aires Oliva-Teles. Effect of dietary ARA/EPA/DHA ratios on growth performance and intermediary metabolism of gilthead sea bream (Sparus aurata) juveniles. Aquaculture 2019, 516, 734644 .
AMA StyleR. Magalhães, Inês Guerreiro, Filipe Coutinho, Sara Moutinho, Sara Sousa, C. Delerue-Matos, Valentina Domingues, R.E. Olsen, Helena Peres, Aires Oliva-Teles. Effect of dietary ARA/EPA/DHA ratios on growth performance and intermediary metabolism of gilthead sea bream (Sparus aurata) juveniles. Aquaculture. 2019; 516 ():734644.
Chicago/Turabian StyleR. Magalhães; Inês Guerreiro; Filipe Coutinho; Sara Moutinho; Sara Sousa; C. Delerue-Matos; Valentina Domingues; R.E. Olsen; Helena Peres; Aires Oliva-Teles. 2019. "Effect of dietary ARA/EPA/DHA ratios on growth performance and intermediary metabolism of gilthead sea bream (Sparus aurata) juveniles." Aquaculture 516, no. : 734644.
This work addresses a technological advance applied to the construction of a magnetogenoassay with electrochemical transduction for the maize taxon-specific (HMGA gene) detection using gold-coated magnetic nanoparticles as nanosized platform. Superparamagnetic core-shell [email protected] nanoparticles (10.4 ± 1.7 nm) were used to assemble the genoassay through the covalent immobilization of HMGA DNA probes onto carboxylated self-assembled monolayers at the nanoparticles surface. A hybridization reaction using sandwich format was selected to prevent inefficient hybridization connected with stable secondary DNA structures using also fluorescein isothiocyanate as DNA signaling tag. The labelling of the hybridization reaction with enzymes allowed the chronoamperometric measurement of the peroxidase activity linked to the nanoplatform located on gold surface. Using this electrochemical magnetogenoassay a linear concentration range from 0.5 to 5 nM and a LOD of 90 pM with a RSD <1.2% was calculated. Certified maize was evaluated without further purification after PCR amplification. This work highlights the efficacy of the electrochemical magnetogenoassay for the HMGA detection, showing its potential as alternative procedure for the verification of the compliance of the legislation.
Juliana Beatriz Sousa; Joilson Ramos-Jesus; L.C. Silva; C. Pereira; N. De-Los-Santos-Álvarez; Rosana A.S. Fonseca; R. Miranda-Castro; C. Delerue-Matos; J. Ribeiro Santos Júnior; M. Fátima Barroso. [email protected] nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing. Talanta 2019, 206, 120220 .
AMA StyleJuliana Beatriz Sousa, Joilson Ramos-Jesus, L.C. Silva, C. Pereira, N. De-Los-Santos-Álvarez, Rosana A.S. Fonseca, R. Miranda-Castro, C. Delerue-Matos, J. Ribeiro Santos Júnior, M. Fátima Barroso. [email protected] nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing. Talanta. 2019; 206 ():120220.
Chicago/Turabian StyleJuliana Beatriz Sousa; Joilson Ramos-Jesus; L.C. Silva; C. Pereira; N. De-Los-Santos-Álvarez; Rosana A.S. Fonseca; R. Miranda-Castro; C. Delerue-Matos; J. Ribeiro Santos Júnior; M. Fátima Barroso. 2019. "[email protected] nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing." Talanta 206, no. : 120220.
S. Sousa; M. L. Maia; L. Correira-Sá; V. C. Fernandes; C. Delerue-Matos; C. Calhau; V. F. Domingues. Chemistry and Toxicology Behind Insecticides and Herbicides. Controlled Release of Pesticides for Sustainable Agriculture 2019, 59 -109.
AMA StyleS. Sousa, M. L. Maia, L. Correira-Sá, V. C. Fernandes, C. Delerue-Matos, C. Calhau, V. F. Domingues. Chemistry and Toxicology Behind Insecticides and Herbicides. Controlled Release of Pesticides for Sustainable Agriculture. 2019; ():59-109.
Chicago/Turabian StyleS. Sousa; M. L. Maia; L. Correira-Sá; V. C. Fernandes; C. Delerue-Matos; C. Calhau; V. F. Domingues. 2019. "Chemistry and Toxicology Behind Insecticides and Herbicides." Controlled Release of Pesticides for Sustainable Agriculture , no. : 59-109.
Pyrethroid insecticides are emerging contaminants broadly used for insect pest control in agriculture, veterinary, and domestic applications. Limited information can be found in literature regarding pyrethroid pesticide soil contamination in playgrounds and agricultural areas. The focus of this study was on new findings related to the spatial and seasonal occurrence of pyrethroid pesticide contamination in soils of northern Portugal. The soils were tested during summer and winter seasons. Pyrethroid pesticides were not found in the ten playgrounds sampled during both seasons. For the 18 agricultural soil samples tested, deltamethrin was the only pyrethroid detected, just during the summer season. For those, three of the samples taken were found to be positive with concentrations between 15.7 and 101.7 ng g−1. The results denote the need for monitoring and assessment of pyrethroid pesticide contamination in Portuguese soil. Further research is needed to access the ecological potential impact of pyrethroid pesticides on soil. Graphical Abstract
Idalina Bragança; Paulo C. Lemos; Cristina Delerue-Matos; Valentina F. Domingues. Assessment of Pyrethroid Pesticides in Topsoils in Northern Portugal. Water, Air, & Soil Pollution 2019, 230, 1 -10.
AMA StyleIdalina Bragança, Paulo C. Lemos, Cristina Delerue-Matos, Valentina F. Domingues. Assessment of Pyrethroid Pesticides in Topsoils in Northern Portugal. Water, Air, & Soil Pollution. 2019; 230 (7):1-10.
Chicago/Turabian StyleIdalina Bragança; Paulo C. Lemos; Cristina Delerue-Matos; Valentina F. Domingues. 2019. "Assessment of Pyrethroid Pesticides in Topsoils in Northern Portugal." Water, Air, & Soil Pollution 230, no. 7: 1-10.
A green synthetic route was developed to prepare silver nanoparticles (AgNPs) in aqueous solution for biological applications. Eschweilenol C, a compound derivative ellagic acid was identified as the main constituent of the aqueous fraction of the ethanolic extract of Terminalia fagifolia Mart. by NMR analysis. In the green synthesis, the ethanolic extract of T. fagifolia and its aqueous fraction were used to promote silver reduction and nanoparticle stabilization. The synthesized AgNPs presented a spherical or polygonal morphology shape by TEM analysis and AgNPs showed high levels of antioxidant and considerable antibacterial and antifungal activities. Synthesized nanoparticles presented significant antioxidant activity by sequestration of DPPH and ABTS radicals, in addition to iron reduction (FRAP assay) and measurement of antioxidant capacity in ORAC units, in addition, AgNP synthesized with the aqueous fraction also demonstrated antioxidant potential in microglial cells. Gram-positive and Gram-negative bacteria were susceptible to growth inhibition by the nanoparticles, among which the AgNPs formed by the ethanolic extract was the most effective. The data obtained by AFM images suggested that AgNPs could lead to the lysis of bacteria and subsequent death. The antifungal assays showed high efficiency against yeasts and dermatophytes. This work represents the first description of antifungal activity by AgNPs against Fonsecaea pedrosoi, the etiologic agent of chromoblastomycosis. In relation to biocompatibility, the AgNPs induced lower haemolysis than AgNO3.
Alyne Rodrigues de Araujo; Joilson Ramos-Jesus; Taiane Maria de Oliveira; Andressa Maria A. de Carvalho; Paulo Humberto M. Nunes; Tatiane Caroline Daboit; Ana Carvalho; M. Fátima Barroso; Miguel Peixoto de Almeida; Alexandra Plácido; Artur Rodrigues; Camila Portugal; Renato Socodato; João B. Relvas; Cristina Delerue-Matos; Durcilene Silva; Peter Eaton; José Roberto De Souza De A. Leite. Identification of Eschweilenol C in derivative of Terminalia fagifolia Mart. and green synthesis of bioactive and biocompatible silver nanoparticles. Industrial Crops and Products 2019, 137, 52 -65.
AMA StyleAlyne Rodrigues de Araujo, Joilson Ramos-Jesus, Taiane Maria de Oliveira, Andressa Maria A. de Carvalho, Paulo Humberto M. Nunes, Tatiane Caroline Daboit, Ana Carvalho, M. Fátima Barroso, Miguel Peixoto de Almeida, Alexandra Plácido, Artur Rodrigues, Camila Portugal, Renato Socodato, João B. Relvas, Cristina Delerue-Matos, Durcilene Silva, Peter Eaton, José Roberto De Souza De A. Leite. Identification of Eschweilenol C in derivative of Terminalia fagifolia Mart. and green synthesis of bioactive and biocompatible silver nanoparticles. Industrial Crops and Products. 2019; 137 ():52-65.
Chicago/Turabian StyleAlyne Rodrigues de Araujo; Joilson Ramos-Jesus; Taiane Maria de Oliveira; Andressa Maria A. de Carvalho; Paulo Humberto M. Nunes; Tatiane Caroline Daboit; Ana Carvalho; M. Fátima Barroso; Miguel Peixoto de Almeida; Alexandra Plácido; Artur Rodrigues; Camila Portugal; Renato Socodato; João B. Relvas; Cristina Delerue-Matos; Durcilene Silva; Peter Eaton; José Roberto De Souza De A. Leite. 2019. "Identification of Eschweilenol C in derivative of Terminalia fagifolia Mart. and green synthesis of bioactive and biocompatible silver nanoparticles." Industrial Crops and Products 137, no. : 52-65.