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Itzel C. Romero-Soto
Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico

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Journal article
Published: 12 August 2021 in Sustainability
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In the present study, a novel laccase from ascomycete Gliomastix murorum was produced in agro-industrial wastes and entrapped in galactomannan beads for Reactive Blue 2 (Rb-2) decolorization. The maximum laccase production in agave bagasse-based medium occurred at 72 h (1798.6 UL−1). Entrapped laccase decolorized ˃80% of 0.5 mM Rb-2 in 2 h without the addition of redox mediator. Km for Rb-2 substrate was 1.42 mM, with a Vmax of 1.19 µmol min−1 for entrapped laccase. Galactomannan matrices produce stability to acid pH (2–5) and temperatures from 20–70 °C. Reusability assays showed that entrapped laccase could retain efficient Rb-2 decolorization of ˃80% six times. In general, galactomannan used for entrapment of laccase provides economic advantages in large-scale wastewater treatment due to its natural origin and efficient results.

ACS Style

Itzel C. Romero-Soto; Raúl B. Martínez-Pérez; Jorge A. Rodríguez; Rosa M. Camacho-Ruiz; Alejandra Barbachano-Torres; Martha Martín del Campo; Juan Napoles-Armenta; Jorge E. Pliego-Sandoval; María O. Concha-Guzmán; María Angeles Camacho-Ruiz. Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization. Sustainability 2021, 13, 9019 .

AMA Style

Itzel C. Romero-Soto, Raúl B. Martínez-Pérez, Jorge A. Rodríguez, Rosa M. Camacho-Ruiz, Alejandra Barbachano-Torres, Martha Martín del Campo, Juan Napoles-Armenta, Jorge E. Pliego-Sandoval, María O. Concha-Guzmán, María Angeles Camacho-Ruiz. Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization. Sustainability. 2021; 13 (16):9019.

Chicago/Turabian Style

Itzel C. Romero-Soto; Raúl B. Martínez-Pérez; Jorge A. Rodríguez; Rosa M. Camacho-Ruiz; Alejandra Barbachano-Torres; Martha Martín del Campo; Juan Napoles-Armenta; Jorge E. Pliego-Sandoval; María O. Concha-Guzmán; María Angeles Camacho-Ruiz. 2021. "Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization." Sustainability 13, no. 16: 9019.