Monitoring infrastructures is becoming an important and challenging issue. In Italy, the heritage consists of more than 60,000 bridges, which need to be inspected and detected in order to guarantee their strength and durability function during nominal lifespan. In this paper, a non-destructive survey methodology for study concrete bridges surface deterioration and viaducts is presented. Terrestrial and unmanned aerial vehicle (UAV) photogrammetry has been used for visual inspection of a standard concrete overpass in L’Aquila (Italy). The obtained orthomosaic has been processed by means of Object-Based Image Analysis (OBIA) to identify and classify deteriorated areas and decay forms. The results show a satisfactory identification and survey of deteriorated areas. It has also been possible to quantify metric information, such as width and length of cracks and extension of weathered areas. This allows to perform easy and fast periodic inspections over time in order to evaluate the evolution of deterioration and plan urgency of preservation or maintenance measures.

Filiberto Mastrangelo, MD, DDS, PhD/Raimondo Quaresima/Luigi Canullo, DDS/Antonio Scarano, MD/Lorenzo Lo Muzio, MD/Adriano Piattelli, MD: Purpose: The aim of this study was to compare how two innovative laser titanium surfaces and sandblasted and acid-etched surfaces influence human osteoblast behavior during osteogenesis and the initial phases of bone deposition. Materials and Methods: Human osteoblasts from human adipose stem cells were sorted by flow cytometric analysis and induced to differentiate. After 40 days, the osteogenic differentiation was detected by alizarin red staining, and the alkaline phosphatase (ALP) was evaluated with western blot (WB) and real-time reverse transcriptase-polymerase chain reaction (RT-PcR) analysis. After confluence, human osteoblasts were cultured onto two different innovative laser-obtained titanium surfaces (L1 and L2) and compared with one sandblasted and acid-etched (SBAE) surface as the control. At different times, human osteoblast behavior was evaluated with cell proliferation viability assay (MTT), scanning electron microscopy (SEM), energy-dispersive x-rays (EDAX), osteogenic markers with RT-PcR, and WB analysis of matrix extracellular phosphoglycoprotein (MEPE), ALP, and osteocalcin (OCN). Results: After cell sorting, the human osteoblasts from human adipose stem cells showed increasing values of ALP mRNA and protein expression. The osteogenic differentiation was confirmed by quantitative alizarin red staining assay. Profilometric and SEM analysis showed relevant differences between SBAE, L1, and L2 specimens. After 20 days of culture onto titanium samples, SEM evaluation showed a small number of human osteoblasts and isolated sites of bone matrix deposition in SBAE specimens. At the same time, on L1 surfaces, only an osteoblast mono-layer with initial bone deposition was found, while on L2 specimens, there was a thick network with flattened large stellate cells, many cellular interconnections with strong titanium adhesion, and a large complex mineralized structure of crystal bone. After 20 days, for all titanium samples, human osteoblasts culturing EDAX analysis showed the absence of impurities and a higher bone matrix deposition in L2 specimens compared with L1 and SBAE samples. Conclusion: The innovative microtopography and nanotopography laser-induced surface showed high biocompatibility with primary human osteoblast cultures and the absence of impurities. The innovative laser texture was capable of influencing the osteogenic process, confirming the critical role of titanium surface characteristics in the cell adhesion and bone deposition during the early phases of osseointegration. The association of human adipose stem cells and titanium surfaces laser-induced with an innovative procedure could generate promising improvements and developments in orthopedics, maxillofacial, and dental implant surgery.

White adipose tissue is a source of mesenchymal stromal/stem cells (MSCs) that are actively studied for their possible therapeutic use in bone tissue repair/remodeling. To better appreciate the osteogenic potential of these cells, we compared some properties of MSCs from human subcutaneous adipose tissue [subcutaneous-adipose stromal cells (S-ASCs)] and dental pulp stem cell (DPSCs) of third-impacted molars, the latter representing a well-established MSC source. Both undifferentiated cell types showed similar fibroblast-like morphology and mesenchymal marker expression. However, undifferentiated S-ASCs displayed a faster doubling time coupled to greater proliferation and colony-forming ability than DPSCs. Also, the osteogenic differentiation of S-ASCs was greater than that of DPSCs, as evaluated by the higher levels of expression of early osteogenic markers Runt-related transcription factor-2 (RUNX2) and alkaline phosphatase at days 3–14 and of extracellular matrix mineralization at days 14–21. Moreover, S-ASCs showed a better colonization of the titanium scaffold. In addition, we investigated whether S-ASC osteogenic commitment was enhanced by adenosine A1 receptor (A1R) stimulation, as previously shown for DPSCs. Although A1R expression was constant during DPSC differentiation, it increased in S-ASC at day 21 from osteogenesis induction. Accordingly, A1R stimulation by the agonist 2-chloro-N⁶-cyclopentyl-adenosine, added to the cultures at each medium change, stimulated proliferation only in differentiating DPSC and enhanced the osteogenic differentiation earlier in DPSCs than in S-ASCs. These effects were counteracted by cell pretreatment with a selective A1R antagonist. Thus, our findings suggest that S-ASCs could be advantageously used in regenerative orthopedics/dentistry, and locally released or exogenously added purines may play a role in bone repair/remodeling, even though this aspect should be more thoroughly evaluated.

Over the last decades, remote sensing techniques have contributed to supporting cultural heritage studies and management, including archaeological sites as well as their territorial context and geographical surroundings. This paper aims to investigate the capabilities and limitations of the new hyperspectral sensor PRISMA (Precursore IperSpettrale della Missione Applicativa) by the Italian Space Agency (ASI), still little applied to archaeological studies. The PRISMA sensor was tested on Italian terrestrial (Alba Fucens, Massa D’Albe, L’Aquila) and marine (Sinuessa, Mondragone, Caserta) archaeological sites. A comparison between PRISMA hyperspectral imagery and the well-known Sentinel-2 Multi-Spectral Instrument (MSI) was performed in order to better understand features and outputs useful to investigate the aforementioned areas. At first, bad bands analysis and noise removal were performed, in order to delete the numerically corrupted bands. Principal component analysis (PCA) was carried out to highlight invisible details in the original image; then, spectral signatures of representative areas were extracted and compared to Sentinel-2 data. At last, a classification analysis (ML and SAM) was performed both on PRISMA and Sentinel-2 imagery. The results showed a full agreement between Sentinel and PRISMA data, enhancing the capability of PRISMA in extrapolating more spectral information and providing a better reliability in the extraction of the features.