The detection, quantification, diagnosis, and identification of plant diseases is particularly crucial for precision agriculture. Recently, traditional visual assessment technology has not been able to meet the needs of precision agricultural informatization development, and hyperspectral technology, as a typical type of non-invasive technology, has received increasing attention. On the basis of simply describing the types of pathogens and host–pathogen interaction processes, this review expounds the great advantages of hyperspectral technologies in plant disease detection. Then, in the process of describing the hyperspectral disease analysis steps, the articles, algorithms, and methods from disease detection to qualitative and quantitative evaluation are mainly summarizing. Additionally, according to the discussion of the current major problems in plant disease detection with hyperspectral technologies, we propose that different pathogens’ identification, biotic and abiotic stresses discrimination, plant disease early warning, and satellite-based hyperspectral technology are the primary challenges and pave the way for a targeted response.

Accurate counting of wheat ears in field conditions is vital to predict yield and for crop breeding. To quickly and accurately obtain the number of wheat ears in a field, we propose herein a method to count wheat ears based on fully convolutional network (FCN) and Harris corner detection. The technical procedure consists essentially of 1) constructing a dataset of wheat-ear images from acquired red-green-blue (RGB) images; 2) training a FCN as the wheat-ear segmentation model by using the constructed image dataset; 3) preparing testing images and inputting them into the segmentation model to get the initial segmentation results; 4) binarizing the initial segmentation by using the Otsu algorithm (to facilitate subsequent processing); and 5) applying Harris corner detection after extracting the wheat-ear skeleton to obtain the number of wheat ears in the images. The segmentation results show that the proposed FCN-based segmentation model segments wheat ears with an average accuracy of 0.984 and at low computational cost. An average of only 0.033 s is required to segment a $256\times 256$ -pixel wheat-ear image. Moreover, the segmentation result is improved by nearly 10% compared with the previous segmentation methods under conditions of wheat-ear occlusion, leaf occlusion, uneven illumination, and soil disturbance. Subsequently, the proposed counting method achieves good results, with an average accuracy of 0.974, a coefficient of determination (R ² ) of 0.983, and a root mean square error (RMSE) of 14.043. These metrics are all improved by 10% compared with the previous methods. These results show that the proposed method accurately counts wheat ears even under conditions of wheat-ear adhesion. Furthermore, the results provide an important technique for studying wheat phenotyping.

As a quarantine disease with a rapid spread tendency in the context of climate change, accurate detection and location of pine wilt disease (PWD) at different infection stages is critical for maintaining forest health and being highly productivity. In recent years, unmanned aerial vehicle (UAV)-based optical remote-sensing images have provided new instruments for timely and accurate PWD monitoring. Numerous corresponding analysis algorithms have been proposed for UAV-based image classification, but their applicability of detecting different PWD infection stages has not yet been evaluated under a uniform conditions and criteria. This research aims to systematically assess the performance of multi-source images for detecting different PWD infection stages, analyze effective classification algorithms, and further analyze the validity of thermal images for early detection of PWD. In this study, PWD infection was divided into four stages: healthy, chlorosis, red and gray, and UAV-based hyperspectral (HSI), multispectral (MSI), and MSI with a thermal band (MSI&TIR) datasets were used as the data sources. Spectral analysis, support vector machine (SVM), random forest (RF), two- and three-dimensional convolutional network (2D- and 3D-CNN) algorithms were applied to these datasets to compare their classification abilities. The results were as follows: (I) The classification accuracy of the healthy, red, and gray stages using the MSI dataset was close to that obtained when using the MSI&TIR dataset with the same algorithms, whereas the HSI dataset displayed no obvious advantages. (II) The RF and 3D-CNN algorithms were the most accurate for all datasets (RF: overall accuracy = 94.26%, 3D-CNN: overall accuracy = 93.31%), while the spectral analysis method is also valid for the MSI&TIR dataset. (III) Thermal band displayed significant potential in detection of the chlorosis stage, and the MSI&TIR dataset displayed the best performance for detection of all infection stages. Considering this, we suggest that the MSI&TIR dataset can essentially satisfy PWD identification requirements at various stages, and the RF algorithm provides the best choice, especially in actual forest investigations. In addition, the performance of thermal imaging in the early monitoring of PWD is worthy of further investigation. These findings are expected to provide insight into future research and actual surveys regarding the selection of both remote sensing datasets and data analysis algorithms for detection requirements of different PWD infection stages to detect the disease earlier and prevent losses.

Pine wilt disease (PWD) is a great danger, due to two aspects: no effective cure and fast dissemination. One key to the prevention and treatment of pine wilt disease is the early detection of infected wood. Subsequently, appropriate treatment can be applied to limit the further spread of pine wilt disease. In this work, a UAV (Unmanned Aerial Vehicle) with a RGB (Red, Green, Blue) camera was employed as it provided high-quality images of pine trees in a timely manner. Seven flights were performed above seven sample plots in northwestern Beijing, China. Then, raw images captured by the UAV were further pre-processed, classified, annotated, and formed the research datasets. In the formal analysis, improved YOLOv5 frameworks that integrated four attention mechanism modules, i.e., SE (Squeeze-and-Excitation), CA (Coordinate Attention), ECA (Efficient Channel Attention), and CBAM (Convolutional Block Attention Module), were developed. Each of them had been shown to improve the overall identification rate of infected trees at different ranges. The CA module was found to have the best performance, with an accuracy of 92.6%, a 3.3% improvement over the original YOLOv5s model. Meanwhile, the recognition speed was improved by 20 frames/second compared to the original YOLOv5s model. The comprehensive performance could well support the need for rapid detection of pine wilt disease. The overall framework proposed by this work shows a fast response to the spread of PWD. In addition, it requires a small amount of financial resources, which determines the duplication of this method for forestry operators.

Exact yield estimation of fruits on plants guaranteed fine and timely decisions on harvesting and marketing practices. Automatic yield estimation based on unmanned agriculture offers a viable solution for large orchards. Recent years have witnessed notable progress in computer vision with deep learning for yield estimation. Yet, the current practice of vision-based yield estimation with successive frames may engender fairly great error because of the double counting of repeat fruits in different images. The goal of this study is to provide a wise framework for fruit yield estimation in sequence images. Specifically, the anchor-free detection architecture (CenterNet) is utilized to detect fruits in sequence images from videos collected in the apple orchard and orange orchard. In order to avoid double counts of a single fruit between different images in an image sequence, the patch matching model is designed with the Kuhn–Munkres algorithm to optimize the paring process of repeat fruits in a one-to-one assignment manner for the sound performance of fruit yield estimation. Experimental results show that the CenterNet model can successfully detect fruits, including apples and oranges, in sequence images and achieved a mean Average Precision (mAP) of 0.939 under an IoU of 0.5. The designed patch matching model obtained an F1-Score of 0.816 and 0.864 for both apples and oranges with good accuracy, precision, and recall, which outperforms the performance of the reference method. The proposed pipeline for the fruit yield estimation in the test image sequences agreed well with the ground truth, resulting in a squared correlation coefficient of R²_apple = 0.9737 and R²_orange = 0.9562, with a low Root Mean Square Error (RMSE) for these two varieties of fruit.