This study presents the first findings regarding extraction, isolation, enzyme inhibition, and antioxidant activity. The oral mucosal wound‐healing process was investigated using propolis water extract (PWE) incubation with gingival fibroblast cells and concluded that propolis was effective on the oral mucosal wound‐healing pattern compared to untreated controls. Additionally, phenolic compounds (fraxetin, apigenin, galangin, pinobanksin, chrysin, etc.) were isolated from propolis, and their chemical structures were elucidated using comprehensive spectroscopic methods. The antioxidant and anti‐Alzheimer potential activities of PWE and some isolated compounds were screened and revealing their inhibitory effects on acetylcholinesterase (AChE) with IC₅₀ values ranging from 0.45 ± 0.01 to 1.15 ± 0.03 mM, as well as remarkable free‐radical scavenging and metal reduction capacities. The results suggest that these compounds and PWE can be used as therapeutic agents due to their antioxidant properties and inhibitory potential on AChE. It can also be used for therapeutic purposes since its wound‐healing effect is promising.

Objective: For in vitro tissue engineering in urology, stem cells are commonly isolated from tissue specimens obtained during open or endoscopic surgery. Within the context of the present study our aim was to isolate cells from human urine by an alternative and safe technique rather than using the indicated method. Material and methods: After human urine samples had been collected from young and healthy donors via urethral catheterization, cells were precipitated by centrifugation and cultured. Following this isolation procedure, cells were characterized by immunocytochemical method using specific antibodies. Results: When these cells were characterized by immunocytochemical methods using specific antibodies some of them were positive for mesenchymal stem cell marker CD90 while the others were labelled with urothelial marker cytokeratin 7. When all these results were taken into consideration, urothelial cells together with stem cells were observed in the urine- derived cell population. Conclusion: According to the results obtained from this study human urine may be preferred as an alternative stem cell and urothelial cell source in that this method is and easily reproducible non-invasive method. Cite this article as: Erden Tayhan S, Tezcan Keleş G, Topçu İ, Mir E, Deliloğlu Gürhan Sİ. Isolation and in vitro cultivation of human urinederived cells: an alternative stem cell source. Turk J Urol 2017; 43: 345-9

Herbal medicines are being used for primary health care due to their efficacy, safety and less side effects. Teucrium genus is a member of the Lamiaceae family, which is a medicinal plant have been used in traditional medicine, especially for wound healing and inflammatory conditions. Teucroside, 9′-decarboxyrosmarinic acid-4′-O-α-rhamnosyl-(1‴→6‴)-O-β-galactosyl-(1‴→4″)-O-α-rhamnoside is a natural phenolic compund which is isolated and identified from of Teucrium genus. In this study, because of bioactive properties of Teucrioside, it was decided to examine its potential wound healing effect. Wound healing process was investigated by in vitro scratch assay which was an easy, inexpensive and well developed method to measure cell migration. In this context, firstly, cell viability was determined by MTT assay and the results were evaluated to find effective concentration for wound healing. Then the cells were incubated for 48 h with extract with defined concentration. Finally, after 48 hours of incubation with teucroside, the wound healing was calculated as 47%. When the data were compared with untreated control (49%), it was concluded that teucroside had not wound healing potential.

The purpose of the present study was in vitro determination of the combined effects of doxorubucin and 5-fluorouracil by 2D and 3D culture conditions on breast cancer using MCF-7 cell line and CSCs isolated from these cells. In the first stage of this study, CSC isolation and their characterization were performed. In the next experimental period, the antiproliferative effects of 5-Fu and Dox on the MCF-7 and CSCs were demonstrated on 2D. To evaluate the synergistic/antagonistic effects of these chemotherapeutics, the CI was calculated. Additionally, 3D tumor spheroids were used as another model. In the last step, qRT-PCR analysis was performed to examine apoptosis-related gene expressions. In this study, it was clearly seen that CSCs obtained from the breast cancer cell line express stemness factors. In addition, the antiproliferative effects of 5-Fu and Dox on breast cancer and associated CSCs were very clear. Their synergistic effects were determined by CI values. Moreover, it was seen that combined theraphy changed the expression levels of genes related to apoptosis. Additionally, it was molecularly demonstrated that 3D tumoroids were more resistant than the others. In conclusion, the polychemotherapeutic approach was much more effective than the monotherapy. The fact that this effect was seen not only in breast cancer cells, but also in breast cancer stem cells. In addition, it was very promising that the results obtained were similar in both two-dimensional and three-dimensional tumoroids.