This study presents the first findings regarding extraction, isolation, enzyme inhibition, and antioxidant activity. The oral mucosal wound‐healing process was investigated using propolis water extract (PWE) incubation with gingival fibroblast cells and concluded that propolis was effective on the oral mucosal wound‐healing pattern compared to untreated controls. Additionally, phenolic compounds (fraxetin, apigenin, galangin, pinobanksin, chrysin, etc.) were isolated from propolis, and their chemical structures were elucidated using comprehensive spectroscopic methods. The antioxidant and anti‐Alzheimer potential activities of PWE and some isolated compounds were screened and revealing their inhibitory effects on acetylcholinesterase (AChE) with IC₅₀ values ranging from 0.45 ± 0.01 to 1.15 ± 0.03 mM, as well as remarkable free‐radical scavenging and metal reduction capacities. The results suggest that these compounds and PWE can be used as therapeutic agents due to their antioxidant properties and inhibitory potential on AChE. It can also be used for therapeutic purposes since its wound‐healing effect is promising.

Herbal medicines are being used for primary health care due to their efficacy, safety and less side effects. Teucrium genus is a member of the Lamiaceae family, which is a medicinal plant have been used in traditional medicine, especially for wound healing and inflammatory conditions. Teucroside, 9′-decarboxyrosmarinic acid-4′-O-α-rhamnosyl-(1‴→6‴)-O-β-galactosyl-(1‴→4″)-O-α-rhamnoside is a natural phenolic compund which is isolated and identified from of Teucrium genus. In this study, because of bioactive properties of Teucrioside, it was decided to examine its potential wound healing effect. Wound healing process was investigated by in vitro scratch assay which was an easy, inexpensive and well developed method to measure cell migration. In this context, firstly, cell viability was determined by MTT assay and the results were evaluated to find effective concentration for wound healing. Then the cells were incubated for 48 h with extract with defined concentration. Finally, after 48 hours of incubation with teucroside, the wound healing was calculated as 47%. When the data were compared with untreated control (49%), it was concluded that teucroside had not wound healing potential.