This page has only limited features, please log in for full access.

Dr. Anburaj Jeyaraj
Nanjing Agricultural University

Basic Info


Research Keywords & Expertise

0 microRNA
0 Camellia sinensis
0 tea plant
0 Functional gene analysis
0 MicroRNA expression profile

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 09 March 2021 in Sustainability
Reads 0
Downloads 0

(1) Aims: This study was aimed to investigate the effects of organic and inorganic fertilizer application on the soil nutrients and microbiota in tea garden soil. (2) Method: Illumina Hiseq sequencing technique was conducted to analyze the microbial diversity and density in different fertilizer-applied tea garden soil. (3) Results: The results showed that Acidobacteria, Proteobacteria and Actinobacteria were the predominant bacterial species observed in the tea garden soil. Besides, the relative abundance of Basidiomycota, Ascomycota and Zygomycota fungal species were higher in the tea garden soil. Correlation analysis revealed that Acidibacter and Acidothermus were significantly correlated with chemical properties (such as total organic carbon (TOC), total phosphorus (TP) and available phosphorus (AP) contents) of the tea garden soil. Furthermore, all these microbes were abundant in medium rapeseed cake (MRSC) + green manure (GM) treated tea garden soil. (4) Conclusion: Based on the obtained results, we conclude that the application of MRSC + GM could be a preferred fertilizer to increase the soil nutrients (TOC, TP and AP content) and microbial population in the tea garden soil.

ACS Style

Haiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability 2021, 13, 2967 .

AMA Style

Haiping Fu, Huan Li, Peng Yin, Huiling Mei, Jianjie Li, Pinqian Zhou, Yuanjiang Wang, Qingping Ma, Anburaj Jeyaraj, Kuberan Thangaraj, Xuan Chen, Xinghui Li, Guiyi Guo. Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil. Sustainability. 2021; 13 (5):2967.

Chicago/Turabian Style

Haiping Fu; Huan Li; Peng Yin; Huiling Mei; Jianjie Li; Pinqian Zhou; Yuanjiang Wang; Qingping Ma; Anburaj Jeyaraj; Kuberan Thangaraj; Xuan Chen; Xinghui Li; Guiyi Guo. 2021. "Integrated Application of Rapeseed Cake and Green Manure Enhances Soil Nutrients and Microbial Communities in Tea Garden Soil." Sustainability 13, no. 5: 2967.

Journal article
Published: 22 January 2021 in Scientia Horticulturae
Reads 0
Downloads 0

Anthracnose, caused by Colletotrichum gloeosporioides is one of the most serious diseases of tea plant [Camellia sinensis (L.) O. Kuntze]. MicroRNAs are key modulators of gene expression in defense responses and plant immunity; although, foliar application of exogenous caffeine in anthracnose disease control management has proven to be effective, miRNA-mediated regulatory mechanisms underlying caffeine-induced plant defense response to C. gloeosporioides remain unexplored in tea plant. Using high-throughput-sequencing, 24 miRNA sequencing data sets and 8 degradome data sets were generated from the susceptible cultivar Longjing43 (LJ43) and the resistant cultivar Zhongcha108 (ZC108) leaves treated with CK (Water), C. gloeosporioides-inoculation (CgI), exogenous caffeine (CN) and CgI + CN. Using sRNA sequencing, 424 conserved miRNAs and 417 novel miRNAs were identified; of these, 146 and 130 miRNAs were differentially expressed under CgI + CN treatment in the LJ43 and ZC108, respectively. Degradome sequencing identified 599 targets predicted to be cleaved by 210 conserved and 70 novel miRNAs. Majority of the annotated targets were found to involve in regulation of transcription factors, oxidation-reduction and metabolic process for plant growth and development as well as stress responses in tea plant against C. gloeosporioides stress. The expression pattern of eight miRNAs and their targets were validated by qRT-PCR, and correlation analysis of csn-miR164a_R+1_1ss21AG/NAC-17 and csn-miR396b-5p/GRF-1 showed highly significant negative R-value at 7th dpi under CgI + CN in the LJ43. This study provides important insights into the novel approach of exogenous caffeine-induced miRNAs dynamically exerts its fungicidal activity through regulating JA/ET signaling pathway, thereby accurately switch on LJ43 susceptibility nature to resistance activity against C. gloeosporioides infection.

ACS Style

Anburaj Jeyaraj; Tamilselvi Elango; Ying Yu; Xuefei Chen; Zhongwei Zou; Zhaotang Ding; Zhen Zhao; Xuan Chen; Xinghui Li; Linbo Chen. Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant. Scientia Horticulturae 2021, 279, 109914 .

AMA Style

Anburaj Jeyaraj, Tamilselvi Elango, Ying Yu, Xuefei Chen, Zhongwei Zou, Zhaotang Ding, Zhen Zhao, Xuan Chen, Xinghui Li, Linbo Chen. Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant. Scientia Horticulturae. 2021; 279 ():109914.

Chicago/Turabian Style

Anburaj Jeyaraj; Tamilselvi Elango; Ying Yu; Xuefei Chen; Zhongwei Zou; Zhaotang Ding; Zhen Zhao; Xuan Chen; Xinghui Li; Linbo Chen. 2021. "Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant." Scientia Horticulturae 279, no. : 109914.

Journal article
Published: 29 August 2020 in Journal of Cleaner Production
Reads 0
Downloads 0

An eco-friendly, low toxic and facile synthetic approach was employed to produce superparamagnetic biogenic iron oxide nanoparticles (SPBIONs) using an aqueous extract of tea-pruning waste as a reducing agent under alkaline conditions. As-biosynthesized SPBIONs were extensively characterized by various analytical tools. For instance, XRD pattern showed crystallinity and FTIR spectra revealed the presence of bioactive molecules required for the reduction of iron oxide ions. AFM and FESEM-EDX images showed agglomerated spherical shape with strong signals of iron metallic ions. The average crystallite sizes of SPBIONs were found to be 20 – 35 nm by HRTEM analysis. Zeta potential analysis confirmed that the surface charge of the green synthesized SPBIONs was highly negative (-25.2 mV) and stable. TGA curve reported a weight loss of 14 %, which occurred in SPBIONs over temperatures ranging from 50 °C to 950 °C due to the removal of water molecules and volatile compounds. VSM analysis revealed that SPBIONs exhibited superparamagnetic properties with a high-saturation magnetization value of 11 emu/g. In addition, the antioxidant property of SPBIONs was investigated with 1,1-Diphenyl-2-picrylhydrazyl (DPPH), a free radical assay, and it was seen that 70 μg/mL (IC50) of SPBIONs was able to neutralize the generation of free radicals and oxidative stress. The present study successfully demonstrated that utilization of tea resources for the production of SPBIONs with superparamagnetic and antioxidant properties might be used to design antioxidant agents and other biomedical applications.

ACS Style

Rajiv Periakaruppan; Xuan Chen; Kuberan Thangaraj; Anburaj Jeyaraj; Hoang Ha Nguyen; Ying Yu; Shunkai Hu; Li Lu; Xinghui Li. Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities. Journal of Cleaner Production 2020, 278, 123962 .

AMA Style

Rajiv Periakaruppan, Xuan Chen, Kuberan Thangaraj, Anburaj Jeyaraj, Hoang Ha Nguyen, Ying Yu, Shunkai Hu, Li Lu, Xinghui Li. Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities. Journal of Cleaner Production. 2020; 278 ():123962.

Chicago/Turabian Style

Rajiv Periakaruppan; Xuan Chen; Kuberan Thangaraj; Anburaj Jeyaraj; Hoang Ha Nguyen; Ying Yu; Shunkai Hu; Li Lu; Xinghui Li. 2020. "Utilization of tea resources with the production of superparamagnetic biogenic iron oxide nanoparticles and an assessment of their antioxidant activities." Journal of Cleaner Production 278, no. : 123962.

Review
Published: 16 June 2020 in RNA Biology
Reads 0
Downloads 0

MicroRNAs play a central role in responses to biotic stressors through their interactions with their target mRNAs. Tea plant (Camellia sinensis L.), an important beverage crop, is vulnerable to tea geometrid and anthracnose disease that causes considerable crop loss and tea production worldwide. Sustainable production of tea in the current scenario to biotic factors is major challenges. To overcome the problem of biotic stresses, high-throughput sequencing (HTS) with bioinformatics analyses has been used as an effective approach for the identification of stress-responsive miRNAs and their regulatory functions in tea plant. These stress-responsive miRNAs can be utilized for miRNA-mediated gene silencing to enhance stress tolerance in tea plant. Therefore, this review summarizes the current understanding of miRNAs regulatory functions in tea plant responding to Ectropis oblique and Colletotrichum gloeosporioides attacks for future miRNA research. Also, it highlights the utilization of miRNA-mediated gene silencing strategies for developing biotic stress tolerant tea plant.

ACS Style

Anburaj Jeyaraj; Tamilselvi Elango; Xinghui Li; Guiyi Guo. Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [Camellia sinensis (L.) O. Kuntze]. RNA Biology 2020, 17, 1365 -1382.

AMA Style

Anburaj Jeyaraj, Tamilselvi Elango, Xinghui Li, Guiyi Guo. Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [Camellia sinensis (L.) O. Kuntze]. RNA Biology. 2020; 17 (10):1365-1382.

Chicago/Turabian Style

Anburaj Jeyaraj; Tamilselvi Elango; Xinghui Li; Guiyi Guo. 2020. "Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [Camellia sinensis (L.) O. Kuntze]." RNA Biology 17, no. 10: 1365-1382.

Journal article
Published: 21 November 2017 in BMC Plant Biology
Reads 0
Downloads 0

MicroRNAs (miRNAs) are important for plant growth and responses to environmental stresses via post-transcriptional regulation of gene expression. Tea, which is primarily produced from one bud and two tender leaves of the tea plant (Camellia sinensis), is one of the most popular non-alcoholic beverages worldwide owing to its abundance of secondary metabolites. A large number of miRNAs have been identified in various plants, including non-model species. However, due to the lack of reference genome sequences and/or information of tea plant genome survey scaffold sequences, discovery of miRNAs has been limited in C. sinensis. Using small RNA sequencing, combined with our recently obtained genome survey data, we have identified and analyzed 175 conserved and 83 novel miRNAs mainly in one bud and two tender leaves of the tea plant. Among these, 93 conserved and 18 novel miRNAs were validated using miRNA microarray hybridization. In addition, the expression pattern of 11 conserved and 8 novel miRNAs were validated by stem-loop-qRT-PCR. A total of 716 potential target genes of identified miRNAs were predicted. Further, Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that most of the target genes were primarily involved in stress response and enzymes related to phenylpropanoid biosynthesis. The predicted targets of 4 conserved miRNAs were further validated by 5'RLM-RACE. A negative correlation between expression profiles of 3 out of 4 conserved miRNAs (csn-miR160a-5p, csn-miR164a, csn-miR828 and csn-miR858a) and their targets (ARF17, NAC100, WER and MYB12 transcription factor) were observed. In summary, the present study is one of few such studies on miRNA detection and identification in the tea plant. The predicted target genes of majority of miRNAs encoded enzymes, transcription factors, and functional proteins. The miRNA-target transcription factor gene interactions may provide important clues about the regulatory mechanism of these miRNAs in the tea plant. The data reported in this study will make a huge contribution to knowledge on the potential miRNA regulators of the secondary metabolism pathway and other important biological processes in C. sinensis.

ACS Style

Anburaj Jeyaraj; Xiao Zhang; Yan Hou; Mingzhu Shangguan; Prabu Gajjeraman; Yeyun Li; Chaoling Wei. Genome-wide identification of conserved and novel microRNAs in one bud and two tender leaves of tea plant (Camellia sinensis) by small RNA sequencing, microarray-based hybridization and genome survey scaffold sequences. BMC Plant Biology 2017, 17, 212 .

AMA Style

Anburaj Jeyaraj, Xiao Zhang, Yan Hou, Mingzhu Shangguan, Prabu Gajjeraman, Yeyun Li, Chaoling Wei. Genome-wide identification of conserved and novel microRNAs in one bud and two tender leaves of tea plant (Camellia sinensis) by small RNA sequencing, microarray-based hybridization and genome survey scaffold sequences. BMC Plant Biology. 2017; 17 (1):212.

Chicago/Turabian Style

Anburaj Jeyaraj; Xiao Zhang; Yan Hou; Mingzhu Shangguan; Prabu Gajjeraman; Yeyun Li; Chaoling Wei. 2017. "Genome-wide identification of conserved and novel microRNAs in one bud and two tender leaves of tea plant (Camellia sinensis) by small RNA sequencing, microarray-based hybridization and genome survey scaffold sequences." BMC Plant Biology 17, no. 1: 212.