The transformation of our linear “take-make-waste” system to a cyclic flow of materials and energy is a priority task for society, but the circular use of waste streams from one industry/sector as a material input for another must be completely safe. The need for new advanced technologies and methods ensuring both microbiological safety and the removal of potential chemical residues in used materials and products is urgent. Non-thermal atmospheric plasma (cold atmospheric plasma—CAP) has recently attracted great research interest as an alternative for operative solutions of problems related to safety and quality control. CAP is a powerful tool for the inactivation of different hazardous microorganisms and viruses, and the effective decontamination of surfaces and liquids has been demonstrated. Additionally, the plasma’s active components are strong oxidizers and their synergetic effect can lead to the degradation of toxic chemical compounds such as phenols and azo-dyes.

Plasma agriculture as a novel approach started to gain more attention in the last decade. In this work, the effect of plasma activated water (PAW) prepared with different plasma sources from three types of water on germination and growth of Cucumis melo L. was studied. The best effect of PAW from distilled water on the growth of seedlings was observed when prepared using the surface-wave-sustained microwave discharge. The effect of artificially prepared plasma activated water (ArtPAW) was studied, however, it was found that while ArtPAW may cause similar effects to the plasma PAW, the effect is not as significant and consistent. Further, PAW was prepared from tap and wastewater. It was found that PAW from wastewater has positive effects on both germination of the seeds and the growth of the seedlings. The root elongation of C. melo after application of plasma treated wastewater increased up to 130% compared to the control. Moreover, it was found that plants grown in PAW from wastewater contained significantly higher concentrations of plant nutrients. Therefore, it shows a promising approach for the application of non-thermal plasma in plant fertilization and a method of wastewater reuse after plasma treatment.

In this paper, an investigation of the interaction of a surface-wave-sustained argon plasma torch with liquids is presented. The plasma is produced by an electromagnetic wave traveling along the plasma–dielectric interface, and at the same time, the plasma is a part of this waveguide structure. Because the interaction of the plasma torch with water (liquid) results in modifications of the properties of both the treated water and the plasma itself, a detailed study of the effects in both media is required. The results of the experimental investigation of a surface-wave-sustained argon plasma torch interaction with liquids show significant changes in the plasma parameters, such as the electron excitation temperature T_e and the average rotation temperature T_rot. In addition, mechanical waves are produced both in the meniscus surface and in the plasma torch by the interaction between the plasma torch (ionized gas with charged particles and electric field) and the liquid surface, which is different from the effects produced by a neutral gas jet on a liquid surface. As a result of the plasma–water interaction, the water’s chemical and physical characteristics, such as the water conductivity, pH, and H₂O₂ concentration, are modified. As a possible application for water purification, the performed SWD treatment of model wastewater shows a significant variation in nitrate, ammonium, phosphate, and COD (chemical oxygen demand) concentration as a result of the treatment.

Biological treatment is a key technology in landfill leachate treatment However, often its efficiency is not high enough due to the pollutants in concentrations above the critical ones. The present study aimed to investigate the adaptive responses that occur in activated sludge (AS) during landfill leachate purification. A model process with AS from a municipal wastewater treatment plant and landfill leachate in increasing concentrations was constructed. The data showed that when dilutions 25 and 50 times had been applied the structure of the AS was preserved, but the COD cannot be reduced below 209 mg O₂/L. The feed of undiluted leachate destroyed the AS structure as SVI was reduced to 1 mL/g, biotic index to 1, floc size was greatly reduced and COD remained high (2526 mg O₂/L). The dominant group of protozoa was changed from attached to free-swimming ciliates. An increase of the bacterial groups responsible for the xenobiotics elimination (aerobic heterotrophs, genera Pseudomonas, Acinetobacter, Azoarcus, Thauera, Alcaligenes) was registered. This was accompanied by a significant increase in free bacteria. The obtained data showed that for optimal treatment of this type of water it is necessary to include a combination of biological treatment with another non-biological method (membrane filtration, reverse osmosis, etc.).

The pollution of aquatic ecosystems is a big problem that has its impact on river sediments. In recent decades, an effective solution to this problem has been the application of bioremediation technologies. Nanoremediation is an innovative part of these technologies. We still know little about the efficiency of nanoparticles, especially nanodiamonds, in modelled conditions. The aim of the present study is to investigate the effect of nanodiamonds on the key parameters of modelled bioremediation of river sediments that are polluted with phenol, as well their effect on the structures and functions of microbial communities. An important indicative mechanism that was used is the application of fluorescent in situ hybridization for sediment microbial communities. The results of this study revealed the positive role of nanodiamonds that is associated with their intoxication with high concentrations of phenol. Readaptation was also found, in which the xenobiotic biodegradation potential evolved by increasing the relative proportions of non-culturable bacteria, namely Acinetobacter (at the 144th hour) and Pseudomonas (at the 214th hour). The results can help to find an effective solution to the question of how information from such precise molecular methods and the application of nanodiamonds can be translated into the accessible language of management and bioremediation technologies.

During adaptation to waters that are rich in xenobiotics, biological systems pass through multiple stages. The first one is related to the restructuring of communities, pronounced destruction of the structure, and multiplication of active biodegradants. The purpose of the present research was to describe the microbiome restructuring that occurs during the adaptation stage in landfill leachate treatment. In a model SBR (sequencing batch reactor), a 21-day purification process of landfill leachate was simulated. Wastewater was fed in increasing concentrations. When undiluted leachate entered, the activated sludge structure disintegrated (Sludge Volume Index—4.6 mL/g). The Chemical Oxygen Demand and ammonium nitrogen concentration remained at high values in the influent (2321.11 mgO₂/L and 573.20 mg/L, respectively). A significant amount of free-swimming cells was found, and the number of aerobic heterotrophs and bacteria of the genera Pseudomonas and Acinetobacter increased by up to 125 times. The Azoarcus-Thauera cluster (27%) and Pseudomonas spp. (16%) were registered as the main bacterial groups in the activated sludge. In the changed structure of the microbial community, Gammaproteobacteria, family Rhizobiaceae, class Saccharimonadia were predominantly represented. Among the suspended bacteria, Microbactericeae and Burkholderiaceae, which are known for their ability to degrade xenobiotics, were present in larger quantities. The enzymological analysis demonstrated that the ortho-pathway of cleavage of aromatic structures was active in the community. The described changes in the leachate-purifying microbial community appear to be destructive at the technological level. At the microbiological level, however, trends of initial adaptation were clearly outlined, which, if continued, could provide a highly efficient biodegradation community.