Plant growth-promoting rhizobacteria are known to associate with several cereal crops. The rhizobacterium exerts its function by synthesizing diverse arrays of phytohormones, such as cytokinin (Ck). However, it is difficult to determine the plant growth promotion when a bacterium produces many different kinds of phytohormones. Therefore, to assess the involvement of Ck in growth promotion and activation of antioxidant and physiological systems, we set up this experiment. Wheat seeds (Triticum aestivum L.) were inoculated with Azospirillum brasilense RA−17 (which produces zeatin type Ck) and RA−18 (which failed to produce Ck). Results showed that seed inoculation with RA−17 significantly improved growth and yield-related parameters compared with RA−18. The activity of enzymes, proline contents, and endogenous hormonal levels in wheat kernels were improved considerably with RA−17 than with RA−18. Strain RA−17 enhanced grain assimilation more than strain RA−18 resulting in a higher crop yield. These results suggest that microbial Ck production may be necessary for stimulating plant growth promotion and activating antioxidant and physiological systems in wheat.

Different soil amendments are applied to improve soil properties and to achieve higher crop yield under drought conditions. The objective of the study was to investigate the role of biochar for the improvement of wheat (Triticum aestivum L.) growth and soil biochemical properties under drought conditions. A pot experiment with a completely randomized design was arranged with four replications in a wire house. Drought was imposed on two critical growth stages (tillering and grain filling) and biochar was applied to the soil 10 days before sowing at two different rates (28 g kg⁻¹ and 38 g kg⁻¹). Soil samples were collected to determine the soil properties including soil respiration and enzymatic parameters after crop harvesting. Results showed that water stress negatively affects all biochemical properties of the soil, while biochar amendments positively improved these properties. Application of biochar at 38 g kg⁻¹ provided significantly higher mineral nutrients, Bray P (18.72%), exchangeable-K (7.44%), soil carbon (11.86%), nitrogen mineralization (16.35%), and soil respiration (6.37%) as a result of increased microbial activities in comparison with the 28 g kg⁻¹ rate.