Phytohormones (PHs) play crucial role in regulation of various physiological and biochemical processes that govern plant growth and yield under optimal and stress conditions. The interaction of these PHs is crucial for plant survival under stressful environments as they trigger signaling pathways. Hormonal cross regulation initiate a cascade of reactions which finely tune the physiological processes in plant architecture that help plant to grow under suboptimal growth conditions. Recently, various studies have highlighted the role of PHs such as abscisic acid, salicylic acid, ethylene, and jasmonates in the plant responses toward environmental stresses. The involvement of cytokinins, gibberellins, auxin, and relatively novel PHs such as strigolactones and brassinosteroids in plant growth and development has been documented under normal and stress conditions. The recent identification of the first plant melatonin receptor opened the door to this regulatory molecule being considered a new plant hormone. However, polyamines, which are not considered PHs, have been included in this chapter. Various microbes produce and secrete hormones which helped the plants in nutrient uptake such as N, P, and Fe. Exogenous use of such microbes help plants in correcting nutrient deficiency under abiotic stresses. This chapter focused on the recent developments in the knowledge related to PHs and their involvement in abiotic stresses of anticipation, signaling, cross-talk, and activation of response mechanisms. In view of role of hormones and capability of microbes in producing hormones, we propose the use of hormones and microbes as potential strategy for crop stress management.

Increased problems associated with side effects and bacterial resistance of chemical drugs has prompted the research focus on herbal medicines in the past few decades. In the present investigation, the antimicrobial activity of the various parts of Avicennia marina (AM), a mangrove plant, has been evaluated. The plants were collected from the Jazan area of the Kingdom of Saudi Arabia. Primary extracts of roots, stem, leaves, fruits, and seeds were made in ethanol and fractioned in ethanol, ethyl acetate, petroleum ether, chloroform, and water. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the extracts were determined against Bacillussubtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. It has been observed that the chloroform extract of roots of the AM exhibited inhibitory effects against both S. aureus (MIC = 1.5 ± 0.03 mg/mL) and E. coli (MIC = 1.7 ± 0.01 mg/mL). The ethanolic extract of the AM roots has shown antibacterial activity against Pseudomonas aeruginosa (MIC = 10.8 ± 0.78 mg/mL), Bacillussubtilis (MIC = 6.1 ± 0.27 mg/mL), Staphylococcus aureus (MIC = 2.3 ± 0.08 mg/mL), and Escherichia coli (MIC = 6.3 ± 0.28 mg/mL). The leaf extract of the AM in ethyl acetate showed antibacterial activity against S. aureus and E. coli. Antifungal activity of these extracts was also investigated against Aspergillus fumigatus and Candida albicans. Ethanolic extract of roots and seeds of the AM has shown antifungal activity against Aspergillus fumigatus when applied individually. Ethanolic extract of the AM fruits has shown an inhibitory effect on the growth of Aspergillus fumigatus and Candida albicans. It is suggested that the plant extracts of AM have tremendous antimicrobial activity against a group of microbes, and this effect depends on both the plant part and the solvent used for extraction. Therefore, this plant can be considered to treat various diseases caused by antibiotic-resistant bacteria.

Groundwater contamination poses a severe public health risk in Lahore, Pakistan’s second-largest city, where over-exploited aquifers are the primary municipal and domestic water supply source. This study presents the first comprehensive district-wide assessment of groundwater quality across Lahore using an innovative integrated approach combining geographic information systems (GIS), multi-criteria decision analysis (MCDA), and water quality indexing techniques. The core objectives were to map the spatial distributions of critical pollutants like arsenic, model their impacts on overall potability, and evaluate targeted remediation scenarios. The analytic hierarchy process (AHP) methodology was applied to derive weights for the relative importance of diverse water quality parameters based on expert judgments. Arsenic received the highest priority weight (0.28), followed by total dissolved solids (0.22) and hardness (0.15), reflecting their significance as health hazards. Weighted overlay analysis in GIS delineated localized quality hotspots, unveiling severely degraded areas with very poor index values (>150) in urban industrial zones like Lahore Cantt, Model Town, and parts of Lahore City. This corroborates reports of unregulated industrial effluent discharges contributing to aquifer pollution. Prospective improvement scenarios projected that reducing heavy metals like arsenic by 30% could enhance quality indices by up to 20.71% in critically degraded localities like Shalimar. Simulating advanced multi-barrier water treatment processes showcased an over 95% potential reduction in arsenic levels, indicating the requirement for deploying advanced oxidation and filtration infrastructure aligned with local contaminant profiles. The integrated decision support tool enables the visualization of complex contamination patterns, evaluation of remediation options, and prioritizing risk-mitigation investments based on the spatial distribution of hazard exposures. This framework equips urban planners and utilities with critical insights for developing targeted groundwater quality restoration policies through strategic interventions encompassing treatment facilities, drainage infrastructure improvements, and pollutant discharge regulations. Its replicability across other regions allows for tackling widespread groundwater contamination challenges through robust data synthesis and quantitative scenario modeling capabilities.

Different soil amendments are applied to improve soil properties and to achieve higher crop yield under drought conditions. The objective of the study was to investigate the role of biochar for the improvement of wheat (Triticum aestivum L.) growth and soil biochemical properties under drought conditions. A pot experiment with a completely randomized design was arranged with four replications in a wire house. Drought was imposed on two critical growth stages (tillering and grain filling) and biochar was applied to the soil 10 days before sowing at two different rates (28 g kg⁻¹ and 38 g kg⁻¹). Soil samples were collected to determine the soil properties including soil respiration and enzymatic parameters after crop harvesting. Results showed that water stress negatively affects all biochemical properties of the soil, while biochar amendments positively improved these properties. Application of biochar at 38 g kg⁻¹ provided significantly higher mineral nutrients, Bray P (18.72%), exchangeable-K (7.44%), soil carbon (11.86%), nitrogen mineralization (16.35%), and soil respiration (6.37%) as a result of increased microbial activities in comparison with the 28 g kg⁻¹ rate.