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Prof. Dr. Ahmed El-Shehawi
Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia

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0 promoter methylation
0 transcriptome analysis
0 wheat genomics
0 Regulation of gene expression

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Journal article
Published: 30 July 2021 in Sustainability
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Sunflower (Helianthus annuus L.), a member of the Asteraceae, is one of the major oilseed crops around the world. Charcoal rot caused by Macrophomina phaseolina (Tassi) Goid is the most damaging disease of sunflowers globally. Fungicides are mostly used to control charcoal rot; however, these cause environmental pollution and pose adverse effects on the ecosystem. Therefore, ecofriendly management options are inevitable for the management of charcoal rot disease. Plant mineral nutrition, the use of plant growth-promoting rhizobacteria and biochar have recently been manipulated for the management of different plant diseases. However, the interactive effects of all these treatments have rarely been tested on charcoal rot suppression in sunflowers. This study assessed the influence of sulfur (0 and 2.25 mg/kg) combined with farmyard manure biochar (2%), NPK (20:20:20 mg/kg) and three different plant growth-promoting rhizobacteria (PGPR) strains on the charcoal rot suppression growth, yield, biochemistry and physiology of sunflower. The PGPR strains included in the study were Bacillus sp. strain MR-1/2 (regarded as PGPR1), Achromobacter sp. strain FB-14 (regarded as PGPR2) and Planomicrobium sp. strain MSSA-10 (regarded as PGPR3). The charcoal rot infestation was induced by inoculating the soil with M. phaseolina, and the impacts of the different treatments were studied on the disease infestation, growth, yield, biochemistry and physiology of sunflowers under 0 and 2.25-mg/kg S application. The results revealed that farmyard manure biochar and Planomicrobium sp. strain MSSA-10 in combination with 2.25-mg/kg S proved effective for the management of charcoal rot disease through regulating the antioxidant enzymes’ activities and strengthening the immune system of sunflower plants. The studied health markers (total chlorophyll content and carotenoids) and stress markers (total protein content, catalase and peroxidase) were significantly altered by the applied treatments under 0 and 2.25-mg/kg S applications. The findings of the experiment indicated that both farmyard manure biochar and Planomicrobium sp. strain MSSA-10, combined with 2.25-mg/kg S, could be used to enhance the crop yield and manage charcoal rot disease in sunflowers. Farmyard manure biochar and Planomicrobium sp. strain MSSA-10 are an easy-to-apply, cost-effective, ecofriendly and sustainable option for the management of charcoal rot disease in sunflowers.

ACS Style

Muhammad Ijaz; Abdul Sattar; Ahmad Sher; Sami Ul-Allah; Muhammad Mansha; Kashif Khan; Muhammad Shahzad; Abdullah Al-Sadi; Muhammad Arif; Bandar Aljuaid; Ahmed El-Shehawi; Shahid Farooq. Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.). Sustainability 2021, 13, 8535 .

AMA Style

Muhammad Ijaz, Abdul Sattar, Ahmad Sher, Sami Ul-Allah, Muhammad Mansha, Kashif Khan, Muhammad Shahzad, Abdullah Al-Sadi, Muhammad Arif, Bandar Aljuaid, Ahmed El-Shehawi, Shahid Farooq. Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.). Sustainability. 2021; 13 (15):8535.

Chicago/Turabian Style

Muhammad Ijaz; Abdul Sattar; Ahmad Sher; Sami Ul-Allah; Muhammad Mansha; Kashif Khan; Muhammad Shahzad; Abdullah Al-Sadi; Muhammad Arif; Bandar Aljuaid; Ahmed El-Shehawi; Shahid Farooq. 2021. "Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.)." Sustainability 13, no. 15: 8535.

Journal article
Published: 17 July 2021 in Animals
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Dietary cinnamon has several bioactive compounds with growth-promoting and immunomodulation potential and is suggested for finfish species. This study evaluated the inclusion of cinnamon at 0, 10, 15, and 20 g/kg in European sea bass (Dicentrarchus labrax) diets. After 90 days, the highest final weight, weight gain, specific growth rate, protein efficiency ratio, and the lowest feed conversion ratio were seen in fish treated with 10 g/kg (p< 0.05). Further, the measured growth hormone in the blood indicated that fish treated with 10 g/kg had a higher level than fish 0 and 20 g/kg. After the feeding trial, fish treated with cinnamon at varying levels had higher lipid content than fish before the feeding trial (p< 0.05). Lower Vibrio spp. and Faecal Coliform counts were observed in fish treated with cinnamon than fish fed a cinnamon-free diet (p< 0.05). The hematocrit level was markedly (p< 0.05) increased in fish fed cinnamon at 10 g/kg compared to the control without significant differences with fish fed 15 and 20 g/kg. Hemoglobin was significantly increased in fish treated with cinnamon at 10, 15, and 20 g/kg compared to fish fed a cinnamon-free diet (p< 0.05). Red and white blood cells (RBCs and WBCs) were meaningfully (p< 0.05) increased in fish treated with cinnamon compared with the control. Markedly, fish treated with cinnamon had higher serum total lipids than the control with the highest value in fish treated with 15 g/kg (p< 0.05). The lysozyme activity was markedly higher in fish treated with 15 g cinnamon/kg than fish fed 0, 10, and 20 g/kg (p< 0.05). Moreover, phagocytic activity was significantly higher in fish treated with cinnamon at 10, and 15 g/kg than fish fed 0 and 20 g/kg (p< 0.05). In conclusion, dietary cinnamon is suggested at 10–15 g/kg for achieving the high production and wellbeing of European sea bass.

ACS Style

Mahmoud Habiba; Ebtehal Hussein; Ahmed Ashry; Ahmed El-Zayat; Aziza Hassan; Ahmed El-Shehawi; Hani Sewilam; Hien Van Doan; Mahmoud Dawood. Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax). Animals 2021, 11, 2128 .

AMA Style

Mahmoud Habiba, Ebtehal Hussein, Ahmed Ashry, Ahmed El-Zayat, Aziza Hassan, Ahmed El-Shehawi, Hani Sewilam, Hien Van Doan, Mahmoud Dawood. Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax). Animals. 2021; 11 (7):2128.

Chicago/Turabian Style

Mahmoud Habiba; Ebtehal Hussein; Ahmed Ashry; Ahmed El-Zayat; Aziza Hassan; Ahmed El-Shehawi; Hani Sewilam; Hien Van Doan; Mahmoud Dawood. 2021. "Dietary Cinnamon Successfully Enhanced the Growth Performance, Growth Hormone, Antibacterial Capacity, and Immunity of European Sea Bass (Dicentrarchus labrax)." Animals 11, no. 7: 2128.

Research article
Published: 13 July 2021 in PLOS ONE
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Malnutrition a health disorders arising due to over or low use of minerals, vitamins and nutritional substances required for proper functioning of body tissues and organs. Zinc (Zn) is the most important mineral required for the normal metabolism of plants and humans. Zinc-deficiency is one of the major cause of malnutrition globally. Maize is highly susceptible to Zn-deficiency and inflicts Zn-deficiency to humans and other animals being nourished on it. This study evaluated the effect of zinc-lysine chelate alone (0.1, 0.5, 1.0 and 1.5%) as seed priming and in combination with Zn-solubilizing bacteria (PMEL-1, PMEL-48, PMEL-57and PMEL-71)) on grain biofortification of autumn maize. The Zn accumulation in different parts (roots, stem, leaves, grains and cob pith) was quantified. Results indicated that Zn contents were 18.5% higher in the seeds primed with 1.5% solution of Zn-lysine chelate and inoculation of ZSB strains compared to control treatments. Seed priming with 1.5% Zn-lysine chelate in combination with ZSB inoculation significantly improved cob diameter and cob length by 16.75% and 42% during 2016 and by 11.36% and 34.35% during 2017. The increase in 100 grains weight over control was 18.4% and 15.27% for 2016 and 2017, respectively. The Zn contents were increased by 15.3%, 15.6%, 49.1%, and 33.0% in grain, cob-pith, stemand roots, respectively compared from control. Thus, the combined application of 1.5% Zn-lysine chelates along with ZSB inoculation could be used for combating malnutrition.

ACS Style

Safdar Bashir; Abdul Basit; Rana Nadeem Abbas; Shahbaz Naeem; Saqib Bashir; Niaz Ahmed; Muhammad Saeed Ahmed; Muhammad Zahaib Ilyas; Zubair Aslam; Saqer S. Alotaibi; Ahmed M. El-Shehawi; Yunzhou Li. Combined application of zinc-lysine chelate and zinc-solubilizing bacteria improves yield and grain biofortification of maize (Zea mays L.). PLOS ONE 2021, 16, e0254647 .

AMA Style

Safdar Bashir, Abdul Basit, Rana Nadeem Abbas, Shahbaz Naeem, Saqib Bashir, Niaz Ahmed, Muhammad Saeed Ahmed, Muhammad Zahaib Ilyas, Zubair Aslam, Saqer S. Alotaibi, Ahmed M. El-Shehawi, Yunzhou Li. Combined application of zinc-lysine chelate and zinc-solubilizing bacteria improves yield and grain biofortification of maize (Zea mays L.). PLOS ONE. 2021; 16 (7):e0254647.

Chicago/Turabian Style

Safdar Bashir; Abdul Basit; Rana Nadeem Abbas; Shahbaz Naeem; Saqib Bashir; Niaz Ahmed; Muhammad Saeed Ahmed; Muhammad Zahaib Ilyas; Zubair Aslam; Saqer S. Alotaibi; Ahmed M. El-Shehawi; Yunzhou Li. 2021. "Combined application of zinc-lysine chelate and zinc-solubilizing bacteria improves yield and grain biofortification of maize (Zea mays L.)." PLOS ONE 16, no. 7: e0254647.

Journal article
Published: 06 July 2021 in Saudi Journal of Biological Sciences
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This study was conducted in a demonstrated field in El-Sharkia Governorate, Egypt, during the winter of season 2020. This experiment was conducted to evaluate the leverage of four post-emergence herbicides i.e., Tribenuron-methyl, Clodinafop- propargyl, Pyroxsulam and Pinoxaden) compared to control on total protein content, essential and non-essential amino acids in three wheat cultivars (Shandweel, Sakha 95, and Giza 179). Generally, the use of foliar herbicides led to a significant decrease in essential, non-essential amino acids and protein content. However, Tribenuran-methyl herbicide increased the levels of proline, glycine, arginine, and histidine, but cystine and threonine not affected as compared to control. On the other hand, foliar herbicide application was significantly increased the yield of Shandweel cultivar, physiological and biochemical parameters as compared to the other varieties. The physiological and biochemical models of dual-herbicide-tolerant wheat cultivars add to our understanding of the crop. In today's agricultural system, herbicide tolerance is important for long-term weed management. Therefore, the study recommended the safely usage of Tribenuran-methyl as herbicide foliar.

ACS Style

Ahmed E. El-Sobki; Ahmed M. Saad; Mohamed T. El-Saadony; Amira M. El-Tahan; Ayman E. Taha; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rehab E.M.E. Salem. Fluctuation in amino acids content in Triticum aestivum L. cultivars as an indicator on the impact of post-emergence herbicides in controlling weeds. Saudi Journal of Biological Sciences 2021, 1 .

AMA Style

Ahmed E. El-Sobki, Ahmed M. Saad, Mohamed T. El-Saadony, Amira M. El-Tahan, Ayman E. Taha, Bandar S. Aljuaid, Ahmed M. El-Shehawi, Rehab E.M.E. Salem. Fluctuation in amino acids content in Triticum aestivum L. cultivars as an indicator on the impact of post-emergence herbicides in controlling weeds. Saudi Journal of Biological Sciences. 2021; ():1.

Chicago/Turabian Style

Ahmed E. El-Sobki; Ahmed M. Saad; Mohamed T. El-Saadony; Amira M. El-Tahan; Ayman E. Taha; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rehab E.M.E. Salem. 2021. "Fluctuation in amino acids content in Triticum aestivum L. cultivars as an indicator on the impact of post-emergence herbicides in controlling weeds." Saudi Journal of Biological Sciences , no. : 1.

Journal article
Published: 29 June 2021 in Saudi Journal of Biological Sciences
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An experiment was conducted at Punjab Agricultural University, Ludhiana during 2014–15 and 2015–16, keeping four sowing dates {25th Oct (D1), 10th Nov (D2), 25th Nov (D3) and 10th Dec (D4)} in main plots and five irrigation schedules {irrigation at 15 (FC15), 25 (FC25), 35 (FC35) and 45 (FC45) % depletion of soil moisture from field capacity (FC) and a conventional practice} in sub plots. The objective of the study was to evaluate the performance of CERES-Wheat model for simulating yield and water use under varying planting and soil moisture regimes. The simulated and observed grain yield was higher in D1, with irrigation applied at FC15 as compared to all other sowing date and irrigation regime combinations. Simulated grain yield decreased by 19% with delay in sowing from 25th October to 10th December because of 8% reduction in simulated crop evapotranspiration. Simulated evapotranspiration decreased by 16%, wheat grain yield by 23% and water productivity by 15% in drip irrigation at 45% depletion from field capacity as compared to drip irrigation at 15% of field capacity. It was further revealed that the model performed well in simulating the phenology, water use and yield of wheat.

ACS Style

Eajaz Ahmad Dar; Ajmer Singh Brar; Showket A. Dar; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rizwan Rashid; Zahoor A. Shah; Abrar Yousuf; Mohammad Amin Bhat; Mushtaq Ahmed; Fayaz Ahmad Bahar; Hesham El Enshasy; Marian Brestic; Maria Barboricova; Marek Zivcak; Shahid Farooq; Mohammad Javed Ansari. Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model. Saudi Journal of Biological Sciences 2021, 1 .

AMA Style

Eajaz Ahmad Dar, Ajmer Singh Brar, Showket A. Dar, Bandar S. Aljuaid, Ahmed M. El-Shehawi, Rizwan Rashid, Zahoor A. Shah, Abrar Yousuf, Mohammad Amin Bhat, Mushtaq Ahmed, Fayaz Ahmad Bahar, Hesham El Enshasy, Marian Brestic, Maria Barboricova, Marek Zivcak, Shahid Farooq, Mohammad Javed Ansari. Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model. Saudi Journal of Biological Sciences. 2021; ():1.

Chicago/Turabian Style

Eajaz Ahmad Dar; Ajmer Singh Brar; Showket A. Dar; Bandar S. Aljuaid; Ahmed M. El-Shehawi; Rizwan Rashid; Zahoor A. Shah; Abrar Yousuf; Mohammad Amin Bhat; Mushtaq Ahmed; Fayaz Ahmad Bahar; Hesham El Enshasy; Marian Brestic; Maria Barboricova; Marek Zivcak; Shahid Farooq; Mohammad Javed Ansari. 2021. "Quantitative response of wheat to sowing dates and irrigation regimes using CERES-Wheat model." Saudi Journal of Biological Sciences , no. : 1.

Journal article
Published: 20 June 2021 in Polymers
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The main objective of this work was to evaluate pulp produced by kraft cooking for wood materials (WMT) (Bougainvillea spectabilis, Ficus altissima, and F. elastica) and non-wood materials (NWMT) (Sorghum bicolor and Zea mays stalks) and to study the fungal activity of handsheets treated with Melia azedarach heartwood extract (MAHE) solutions. Through the aforementioned analyses, the ideal cooking conditions were determined for each raw material based on the lignin percentage present. After cooking, pulp showed a decrease in the Kappa number produced from WMT, ranging from 16 to 17. This was in contrast with NWMT, which had Kappa numbers ranging from 31 to 35. A difference in the optical properties of the pulp produced from WMT was also observed (18 to 29%) compared with pulp produced from NWMT (32.66 to 35.35%). As for the evaluation of the mechanical properties, the tensile index of the pulp ranged from 30.5 to 40 N·m/g for WMT and from 44.33 to 47.43 N·m/g for NWMT; the tear index ranged from 1.66 to 2.55 mN·m2/g for WMT and from 4.75 to 5.87 mN·m2/g for NWMT; and the burst index ranged from 2.35 to 2.85 kPa·m2/g for WMT and from 3.92 to 4.76 kPa·m2/g for NWMT. Finally, the double fold number was 3 compared with that of pulp produced from pulp, which showed good values ranging from 36 to 55. In the SEM examination, sheets produced from treated handsheets with extract from MAHE showed no growth of Aspergillus fumigatus over paper discs manufactured from B. speclabilis pulp wood. Pulp paper produced from Z. mays and S. bicolor stalks was treated with 1% MAHE, while pulp paper from F. elastica was treated with 0.50% and 1% MAHE. With the addition of 0.5 or 1% MAHE, Fusarium culmorum showed no increase in growth over the paper manufactured from B. speclabilis, F. altissima, F. elastica and Zea mays pulps with visual inhibition zones found. There was almost no growth of S. solani in paper discs manufactured from pulps treated with 1% MAHE. This is probably due to the phytochemical compounds present in the extract. The HPLC analysis of MAHE identified p-hydroxybenzoic acid, caffeine, rutin, chlorogenic acid, benzoic acid, quinol, and quercetin as the main compounds, and these were present in concentrations of 3966.88, 1032.67, 834.13, 767.81, 660.64, 594.86, and 460.36 mg/Kg extract, respectively. Additionally, due to the importance of making paper from agricultural waste (stalks of S. bicolor and Z. mays), the development of sorghum and corn with high biomass is suggested.

ACS Style

Mohamed Salem; Saqer Alotaibi; Wael Elgat; Ayman Taha; Yahia Fares; Ahmed El-Shehawi; Rehab Ghareeb. Antifungal Activities of Wood and Non-Wood Kraft Handsheets Treated with Melia azedarach Extract Using SEM and HPLC Analyses. Polymers 2021, 13, 2012 .

AMA Style

Mohamed Salem, Saqer Alotaibi, Wael Elgat, Ayman Taha, Yahia Fares, Ahmed El-Shehawi, Rehab Ghareeb. Antifungal Activities of Wood and Non-Wood Kraft Handsheets Treated with Melia azedarach Extract Using SEM and HPLC Analyses. Polymers. 2021; 13 (12):2012.

Chicago/Turabian Style

Mohamed Salem; Saqer Alotaibi; Wael Elgat; Ayman Taha; Yahia Fares; Ahmed El-Shehawi; Rehab Ghareeb. 2021. "Antifungal Activities of Wood and Non-Wood Kraft Handsheets Treated with Melia azedarach Extract Using SEM and HPLC Analyses." Polymers 13, no. 12: 2012.

Original article
Published: 08 June 2021 in Andrologia
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In this study, we estimated the protective role of Moringa oleifera leaf ethanolic extract (MOLE) against obesity-associated testicular dysfunction. Fifty male albino rats were randomly assigned to five groups (n = 10): Group I (basal diet), group II (basal diet plus MOLE orally), group III (high-fat diet—HFD), group IV (HFD plus oral MOLE) and group V (HFD for 8 weeks followed by a basal diet plus oral MOLE for 6 weeks). The study duration extended for 14 weeks. Serum collected to investigate testosterone, FSH and LH levels. Testicular tissues were used to determine levels of SOD, glutathione, catalase and malondialdehyde. Semen was collected to estimate its quality (morphology, motility and concentration). Morphological changes in the testis were investigated by histopathological and immunohistochemical techniques. Compared with both control treatment and MOLE treatment, serum testosterone, FSH, LH, testicular enzymatic catalase, SOD, GSH, survivin immunoreactivity, sperm quality and testicular weight were all significantly decreased in rats treated with HFD, while there were significant increases in testicular malondialdehyde and caspase-3 immunoreactivity. MOLE improved all harmful effects of HFD. Improvements were more pronounced in the protected (G 4) than the treated (G 5) group. MOLE could be a potential solution for obesity-associated fertility problem.

ACS Style

Mohamed E. Alkafafy; Samy M. Sayed; Ahmed M. El‐Shehawi; Samir El‐Shazly; Samy Farouk; Saqer S. Alotaibi; Doaa A. Madkour; Sahar H. Orabi; Hamed T. Elbaz; Mohamed M. Ahmed. Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model. Andrologia 2021, 53, e14126 .

AMA Style

Mohamed E. Alkafafy, Samy M. Sayed, Ahmed M. El‐Shehawi, Samir El‐Shazly, Samy Farouk, Saqer S. Alotaibi, Doaa A. Madkour, Sahar H. Orabi, Hamed T. Elbaz, Mohamed M. Ahmed. Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model. Andrologia. 2021; 53 (8):e14126.

Chicago/Turabian Style

Mohamed E. Alkafafy; Samy M. Sayed; Ahmed M. El‐Shehawi; Samir El‐Shazly; Samy Farouk; Saqer S. Alotaibi; Doaa A. Madkour; Sahar H. Orabi; Hamed T. Elbaz; Mohamed M. Ahmed. 2021. "Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model." Andrologia 53, no. 8: e14126.

Journal article
Published: 31 May 2021 in Agronomy
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Polygonum perfoliatum L. is an aggressive vine, currently invading the Black Sea region, Turkey. However, information about the seed germination biology of this species is scanty. The objective of the current study was to determine the seed germination biology of three naturalized populations of this species. Chemical scarification with 98% sulfuric acid for 30 min followed by cold-wet stratification at 4 °C for 4 weeks effectively released seed dormancy in tested populations. Seeds of all populations required a 12 h photoperiod for the highest germination, while germination under continuous dark and light remained similar. The seeds were able to germinate under a wide range of constant (5–40 °C) and alternating temperatures, pH (3–11), osmotic potential (0 to −1.4 MPa) and salinity (0–500 mM NaCl). However, the peak germination was observed under 20 °C constant and 20/15 °C alternating day/night temperature, and pH 6.8. Seeds of all populations were able to withstand 200 mM salinity and −0.6 MPa osmotic potential. Increasing seed burial depth initially stimulated seedling emergence and then a sharp decline was observed for the seeds buried below >2 cm depth. More than 90% of the seeds were unable to emerge when buried >6 cm depth. Polygonum perfoliatum has a large potential for range expansion; therefore, immediate management of the naturalized populations is warranted. This weed species in agricultural fields can be managed by burying the seeds in deeper soil layers (6 cm), while post-emergence management strategies need to be developed for roadside populations.

ACS Style

Shahid Farooq; Huseyin Onen; Sonnur Tad; Cumali Ozaslan; Samy Mahmoud; Marian Brestic; Marek Zivcak; Milan Skalicky; Ahmed El-Shehawi. The Influence of Environmental Factors on Seed Germination of Polygonum perfoliatum L.: Implications for Management. Agronomy 2021, 11, 1123 .

AMA Style

Shahid Farooq, Huseyin Onen, Sonnur Tad, Cumali Ozaslan, Samy Mahmoud, Marian Brestic, Marek Zivcak, Milan Skalicky, Ahmed El-Shehawi. The Influence of Environmental Factors on Seed Germination of Polygonum perfoliatum L.: Implications for Management. Agronomy. 2021; 11 (6):1123.

Chicago/Turabian Style

Shahid Farooq; Huseyin Onen; Sonnur Tad; Cumali Ozaslan; Samy Mahmoud; Marian Brestic; Marek Zivcak; Milan Skalicky; Ahmed El-Shehawi. 2021. "The Influence of Environmental Factors on Seed Germination of Polygonum perfoliatum L.: Implications for Management." Agronomy 11, no. 6: 1123.

Journal article
Published: 26 May 2021 in Aquaculture Reports
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The present study evaluated the enhancement of the growth rate, intestinal health, immune-related genes, and resistance against suboptimal water temperature in common carp (2.01 ± 0.02 g) by dietary paraprobiotics. Fish were fed dietary heat killed Lactobacillus plantarum (LP20) at 0 (control), 50, and 100 mg/kg for 2 months. After the feeding trial fish were exposed to suboptimal water temperature averaged 13.22 ± 0.44 °C for 48 h. The final body weight, weight gain, specific growth rate, and feed conversion ratio of fish fed LP20 at 100 mg/kg were significantly enhanced (P < 0.05). The villi length and width were increased in fish fed LP20 at 50 and 100 mg/kg when compared to the control (P < 0.05). The crypt depth was increased by increasing LP20 in a dose dependent manner with the highest value in the group of fish fed 100 mg/kg (P < 0.05). The number of goblet cells showed higher number in fish fed LP20 at 50 and 100 mg/kg than the control group (P < 0.05). The expression of catalase (CAT) gene did not show any changes in transcription before the thermal stress however after thermal stress fish fed 50 mg/kg had upregulated CAT followed by those fed 100 mg/kg. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) gene expressions showed upregulations in fish fed 50 mg/kg before the thermal stress but after the thermal stress fish fed 100 mg/kg had the highest level of mRNA transcriptions for GPx and SOD genes. In the same manner, the expression of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and interleukin 10 (IL-10) were significantly upregulated in fish fed LP20 at 50 mg/kg before the thermal stress whereas the expression of these genes was upregulated in fish fed 100 mg/kg. In conclusion, using LP20 at 50−100 mg/kg resulted in enhancing the growth rate, intestinal morphometry, immune and antioxidative related genes. Accordingly, common carp showed high resistance against suboptimal water temperature stress.

ACS Style

Taha Yassine; Malik Mohamed Khalafalla; Mohamed Mamdouh; Zizy I. Elbialy; Abdallah S. Salah; Aly Ahmedou; Ahmed Mamoon; Ahmed M. El-Shehawi; Hien Van Doan; Mahmoud A.O. Dawood. The enhancement of the growth rate, intestinal health, expression of immune-related genes, and resistance against suboptimal water temperature in common carp (Cyprinus carpio) by dietary paraprobiotics. Aquaculture Reports 2021, 20, 100729 .

AMA Style

Taha Yassine, Malik Mohamed Khalafalla, Mohamed Mamdouh, Zizy I. Elbialy, Abdallah S. Salah, Aly Ahmedou, Ahmed Mamoon, Ahmed M. El-Shehawi, Hien Van Doan, Mahmoud A.O. Dawood. The enhancement of the growth rate, intestinal health, expression of immune-related genes, and resistance against suboptimal water temperature in common carp (Cyprinus carpio) by dietary paraprobiotics. Aquaculture Reports. 2021; 20 ():100729.

Chicago/Turabian Style

Taha Yassine; Malik Mohamed Khalafalla; Mohamed Mamdouh; Zizy I. Elbialy; Abdallah S. Salah; Aly Ahmedou; Ahmed Mamoon; Ahmed M. El-Shehawi; Hien Van Doan; Mahmoud A.O. Dawood. 2021. "The enhancement of the growth rate, intestinal health, expression of immune-related genes, and resistance against suboptimal water temperature in common carp (Cyprinus carpio) by dietary paraprobiotics." Aquaculture Reports 20, no. : 100729.

Journal article
Published: 06 May 2021 in Saudi Journal of Biological Sciences
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Iron (Fe) starvation in Strategy II plants is a major nutritional problem causing severe visual symptoms and yield reductions. This prompted us to investigate the physiological and molecular consequences of Fe deficiency responses at an early stage in sorghum plants. The Fe-starved sorghum did not show shoot biomass reduction, but the root length, biomass, and chlorophyll synthesis were severely affected. The chlorophyll a fluorescence analysis showed that the quantum yield efficiency of PSII (Fv/Fm) and photosynthesis performance index (Pi_ABS) in young leaves significantly reduced in response to low Fe. Besides, Fe concentration in root and shoot significantly declined in Fe-starved plants relative to Fe-sufficient plants. Accordingly, this Fe reduction in tissues was accompanied by a marked decrease in PS-release in roots. The qPCR experiment showed the downregulation of SbDMAS2 (deoxymugineic acid synthase 2), SbNAS3 (nicotianamine synthase 3), and SbYSL1 (Fe-phytosiderophore transporter yellow stripe 1) in Fe-deprived roots, suggesting that decreased rhizosphere mobilization of Fe(III)-PS contributes to reduced uptake and long-distance transport of Fe. The cis-acting elements of these gene promoters are commonly responsive to abscisic acid and methyl jasmonate, while SbYSL1 additionally responsive to salicylic acid. Further, antioxidant defense either through metabolites or antioxidant enzymes is not efficient in counteracting oxidative damage in Fe-deprived sorghum. These findings may be beneficial for the improvement of sorghum genotypes sensitive to Fe-deficiency through breeding or transgenic approaches.

ACS Style

Sadia Akter Prity; Ahmed M. El-Shehawi; Mona M. Elseehy; Sharaban Tahura; Ahmad Humayan Kabir. Early-stage iron deficiency alters physiological processes and iron transporter expression, along with photosynthetic and oxidative damage to sorghum. Saudi Journal of Biological Sciences 2021, 28, 4770 -4777.

AMA Style

Sadia Akter Prity, Ahmed M. El-Shehawi, Mona M. Elseehy, Sharaban Tahura, Ahmad Humayan Kabir. Early-stage iron deficiency alters physiological processes and iron transporter expression, along with photosynthetic and oxidative damage to sorghum. Saudi Journal of Biological Sciences. 2021; 28 (8):4770-4777.

Chicago/Turabian Style

Sadia Akter Prity; Ahmed M. El-Shehawi; Mona M. Elseehy; Sharaban Tahura; Ahmad Humayan Kabir. 2021. "Early-stage iron deficiency alters physiological processes and iron transporter expression, along with photosynthetic and oxidative damage to sorghum." Saudi Journal of Biological Sciences 28, no. 8: 4770-4777.

Journal article
Published: 26 April 2021 in Antibiotics
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A total of 300 human fecal samples were collected from febrile neutropenic patients suffering from severe gastroenteritis, followed by identification and serological characterization of recovered isolates. Fifty nontyphoidal Salmonella (NTS) serovars were recovered. A total of serologically identified 50 NTS serovars recovered from poultry of the same geographical area and during the same period as well as one standard strain S. Poona were supplied by the Bacterial Bank of Animal Health Research Institute of Egypt. Antibiogram analysis revealed that the human and poultry serovars exhibited similar antimicrobial resistance patterns against 28 different antimicrobial agents, particularly against ampicillin, cefotaxime, oxytetracycline, and erythromycin. Plasmids harboring blaCTX-m, blaSHV, blaTEM, and aac(6’)-Ib were detected in 11 (22%) and 8 (16%) of human and poultry serovars, respectively. Molecular detection of the most clinically relevant virulence genes and analysis of the associated virulence genotypes proved that the human (n = 11) and poultry serovars (n = 12) shared 11 genotypes. Enterobacterial repetitive intergenic consensus PCR analysis revealed that human and poultry serovars were clustered together in 3 out of the 4 clusters with a similarity index ranged from 0.15 to 1. Since poultry are usually consumed by humans, the presence of resistant bacteria harboring transmissible genetic elements is of great health concern.

ACS Style

Reem A. Youssef; Ahmad M. Abbas; Ahmed M. El-Shehawi; Mona I. Mabrouk; Khaled M. Aboshanab. Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal Salmonella Recovered from Febrile Neutropenic Patients and Poultry in Egypt. Antibiotics 2021, 10, 493 .

AMA Style

Reem A. Youssef, Ahmad M. Abbas, Ahmed M. El-Shehawi, Mona I. Mabrouk, Khaled M. Aboshanab. Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal Salmonella Recovered from Febrile Neutropenic Patients and Poultry in Egypt. Antibiotics. 2021; 10 (5):493.

Chicago/Turabian Style

Reem A. Youssef; Ahmad M. Abbas; Ahmed M. El-Shehawi; Mona I. Mabrouk; Khaled M. Aboshanab. 2021. "Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal Salmonella Recovered from Febrile Neutropenic Patients and Poultry in Egypt." Antibiotics 10, no. 5: 493.

Journal article
Published: 04 March 2021 in Sustainability
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International Potato Center (CIP), -bred potato genotypes produce various yields under heat stress conditions due to being sown late. To explore options for achieving this, a replicated experiment was conducted at the field of Tuber Crops Research Sub-Centre, Bangladesh Agricultural Research Institute, Bogura, Bangladesh to evaluate the performance of fourteen CIP-bred potato genotypes with two controls (Asterix and Granola). The experiment was laid out in a split-plot design with three replications. Several indices were applied to find out the suitable genotypes under heat stress. The plant height increased by 34.61% under heat stress, which was common in all the potato genotypes. Similarly, other yield-participating characters like stem per hill, canopy coverage (%), plant vigor, and tuber number per plant were also increased under heat stress conditions. However, the tuber yield was decreased by 6.30% and 11.41%, respectively when harvested at 70 and 90 days after plantation. Moreover, “CIP-203” yielded the highest (40.66 t ha−1) in non-stressed whereas, “CIP-118” yielded the highest (32.89 t/ha) in stressed conditions. Likewise, the bred “CIP-218” and “CIP-118” performed better under both growing conditions and yielded >35.00 t ha−1. According to a rank-sum test, among the fourteen potato genotypes, “CIP-218”, “LB-7”, “CIP-118”, “CIP-232”, and “CIP-112” were selected as heat-tolerant potatoes and can grow in both growing conditions with higher yield potential.

ACS Style

Abdullah Al Mahmud; M. Alam; Bimal Kundu; Milan Skalicky; M. Rahman; E. Rahaman; Mousumi Sultana; M. Molla; Akbar Hossain; Ahmed El-Shehawi; Marian Brestic; Ayman Sabagh. Selection of Suitable Potato Genotypes for Late-Sown Heat Stress Conditions Based on Field Performance and Stress Tolerance Indices. Sustainability 2021, 13, 2770 .

AMA Style

Abdullah Al Mahmud, M. Alam, Bimal Kundu, Milan Skalicky, M. Rahman, E. Rahaman, Mousumi Sultana, M. Molla, Akbar Hossain, Ahmed El-Shehawi, Marian Brestic, Ayman Sabagh. Selection of Suitable Potato Genotypes for Late-Sown Heat Stress Conditions Based on Field Performance and Stress Tolerance Indices. Sustainability. 2021; 13 (5):2770.

Chicago/Turabian Style

Abdullah Al Mahmud; M. Alam; Bimal Kundu; Milan Skalicky; M. Rahman; E. Rahaman; Mousumi Sultana; M. Molla; Akbar Hossain; Ahmed El-Shehawi; Marian Brestic; Ayman Sabagh. 2021. "Selection of Suitable Potato Genotypes for Late-Sown Heat Stress Conditions Based on Field Performance and Stress Tolerance Indices." Sustainability 13, no. 5: 2770.

Journal article
Published: 18 February 2021 in Sustainability
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Livestock development in rainfed areas is slower due to the inadequate supply of nutritious fodder. Mono-cropping systems also have a negative impact on forage yield and nutrition as cereals are deficient in protein. Hence, there is a dire need to grow cereals with legumes to improve forage yield and quality. Therefore, a two-year field study was undertaken to evaluate winter cereal–legume forage and their mixtures viz. oats (cv. PD2-LV65), barley (Jau-86) and one legume viz. vetch (cv. Languedock) under different tillage systems viz. conventional tillage (moldboard plow+4-cultivation with tines) and conservation tillage (3 - cultivation with tines). Crops were grown in pure stands as well as in mixtures with a 70:30 seeding ratio. The results revealed that the conventional tillage system performed better in terms of numbers of tillers/branches, leaf-to-stem ratio and green fodder yield than the conservation tillage system. However, the conventional and conservation tillage systems did not show a significant difference in terms of crude protein, acid detergent fiber and neutral detergent fiber. In the pure stands and cereal–legume mixtures, the oat–vetch mixture performed better in terms of plant height, leaf-to-stem ratio and green fodder yield. The maximum crude protein content was observed in the oat–vetch mixture, while the maximum acid detergent fiber and neutral detergent fiber were observed in the pure oat stands. In competitive indices, the land-equivalent ratio and competitive ratio showed the advantage of intercropping. In actual yield loss, results showed the positive value of barley and oats in mixtures, which reflects the advantage of intercropping in the rainfed areas. The economic analysis showed a greater net benefit from the conventional tillage than the conservation tillage system under rainfed conditions. On the basis of this investigation, an oat–vetch mixture and the conventional tillage system are recommended for higher tonnage of nutritious fodder in rainfed areas.

ACS Style

Sunyad Sohail; Muhammad Ansar; Milan Skalicky; Allah Wasaya; Walid Soufan; Tauqeer Ahmad Yasir; Ahmed El-Shehawi; Marian Brestic; Mohammad Sohidul Islam; Muhammad Ali Raza; Ayman EL Sabagh. Influence of Tillage Systems and Cereals–Legume Mixture on Fodder Yield, Quality and Net Returns under Rainfed Conditions. Sustainability 2021, 13, 2172 .

AMA Style

Sunyad Sohail, Muhammad Ansar, Milan Skalicky, Allah Wasaya, Walid Soufan, Tauqeer Ahmad Yasir, Ahmed El-Shehawi, Marian Brestic, Mohammad Sohidul Islam, Muhammad Ali Raza, Ayman EL Sabagh. Influence of Tillage Systems and Cereals–Legume Mixture on Fodder Yield, Quality and Net Returns under Rainfed Conditions. Sustainability. 2021; 13 (4):2172.

Chicago/Turabian Style

Sunyad Sohail; Muhammad Ansar; Milan Skalicky; Allah Wasaya; Walid Soufan; Tauqeer Ahmad Yasir; Ahmed El-Shehawi; Marian Brestic; Mohammad Sohidul Islam; Muhammad Ali Raza; Ayman EL Sabagh. 2021. "Influence of Tillage Systems and Cereals–Legume Mixture on Fodder Yield, Quality and Net Returns under Rainfed Conditions." Sustainability 13, no. 4: 2172.

Journal article
Published: 01 November 2020 in Cytology and Genetics
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The COVID-19 corona virus has become a world pandemic which started in December 2019 in Wuhan, China with no confirmed biological source. Various countries reported the genomic sequence of different isolates obtained from infected patients. This allowed us to obtain a number of 38 isolates of full genomic sequences. Alignment of nucleotide (nt) sequence was carried out using Clustal Omega multiple alignment service at the EBI website. Alignment of nt sequence and phylogenetic relationship revealed that the COVID-19 is a new viral strain and its biological source has not been yet detected. The expected orf pattern was different among isolates obtained from the same country or different countries as well as from SARS-CoV isolates or bats CoV suggesting different virus human interaction possibilities during infection and severity. All isolates had the main five orfs (1ab, S, M, N, E), whereas they differed in the expected accessory orfs. Being with the biological source of COVID-19 undetected, the role of human endogenous retrovirus (HERVs) in the regulation of the host cell gene expression or the encoding for products that could modulate COVID-19 infection and the spectrum of its symptoms is discussed.

ACS Style

Ahmed M. El-Shehawi; Saqer S. Alotaibi; Mona M. Elseehy. Genomic Study of COVID-19 Corona Virus Excludes Its Origin from Recombination or Characterized Biological Sources and Suggests a Role for HERVS in Its Wide Range Symptoms. Cytology and Genetics 2020, 54, 588 -604.

AMA Style

Ahmed M. El-Shehawi, Saqer S. Alotaibi, Mona M. Elseehy. Genomic Study of COVID-19 Corona Virus Excludes Its Origin from Recombination or Characterized Biological Sources and Suggests a Role for HERVS in Its Wide Range Symptoms. Cytology and Genetics. 2020; 54 (6):588-604.

Chicago/Turabian Style

Ahmed M. El-Shehawi; Saqer S. Alotaibi; Mona M. Elseehy. 2020. "Genomic Study of COVID-19 Corona Virus Excludes Its Origin from Recombination or Characterized Biological Sources and Suggests a Role for HERVS in Its Wide Range Symptoms." Cytology and Genetics 54, no. 6: 588-604.

Journal article
Published: 30 October 2020 in Animals
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Monosodium glutamate (MSG) is a widely used food additive, and there is a trepidation that MSG plays a critical role in multiple hepatic disorders. This study was planned to investigate Graviola extract (GE) effects on hepatic and cellular alterations induced by MSG. Fifty Wistar rats were randomly allocated into five groups: control (received normal saline), Graviola (received 200 mg/kg body weight), MSG (received 2.4 gm MSG/kg, 15% of Lethal dose (LD50) of MSG), Graviola + monosodium glutamate (MSG + GE; received GE, 200 mg/kg/day and MSG 2.4 gm/kg body weight (BW) for the next four weeks), and monosodium glutamate + Graviola (received MSG only (2.4 gm/kg BW) daily for four weeks, then concomitant with Graviola (200 mg/kg BW) daily for the next four weeks. MSG and GR were administered orally for eight weeks. Our results showed that MSG caused a significant increase in oxidative stress markers malondialdehyde (MDA), reactive oxygen species (ROS), nitric oxide (NO), hydrogen peroxide (H2O2), proinflammatory cytokines interleukin 6 (IL-6) level, a tumor protein (P53), hepatic cellular damage, as well as proapoptotic markers caspase-3, and B-cell lymphoma 2 (BCL-2)-like protein 4 (Bax). A significant decrease in superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST), reduced glutathione (GSH), and an antiapoptotic agent B-cell lymphoma 2 (BCl-2) was observed. The detected MSG effects were normalized by Graviola administration, either a prophylactic or protecting dose. Besides, Graviola reduced the expression of inducible nitric oxide synthase (iNOS) and hepatic fatty acid synthase (FAS) and led to the upregulation of the silent information regulator protein one gene expression gene (SIRT1).In conclusion, the results suggest that Gaviola’s interrelated antiapoptotic, antioxidant, and anti-inflammatory properties are potential mechanisms to enhance hepatic deficits and protect the liver. Graviola can, therefore, be considered a promising hepatoprotective supplement. Additionally, further human clinical trials are also necessary to validate the present research.

ACS Style

Mustafa Shukry; Ahmed El-Shehawi; Wafaa El-Kholy; Rasha Elsisy; Hazem Hamoda; Hossam Tohamy; Mohamed Abumandour; Foad Farrag. Ameliorative Effect of Graviola (Annona muricata) on Mono Sodium Glutamate-Induced Hepatic Injury in Rats: Antioxidant, Apoptotic, Anti-inflammatory, Lipogenesis Markers, and Histopathological Studies. Animals 2020, 10, 1996 .

AMA Style

Mustafa Shukry, Ahmed El-Shehawi, Wafaa El-Kholy, Rasha Elsisy, Hazem Hamoda, Hossam Tohamy, Mohamed Abumandour, Foad Farrag. Ameliorative Effect of Graviola (Annona muricata) on Mono Sodium Glutamate-Induced Hepatic Injury in Rats: Antioxidant, Apoptotic, Anti-inflammatory, Lipogenesis Markers, and Histopathological Studies. Animals. 2020; 10 (11):1996.

Chicago/Turabian Style

Mustafa Shukry; Ahmed El-Shehawi; Wafaa El-Kholy; Rasha Elsisy; Hazem Hamoda; Hossam Tohamy; Mohamed Abumandour; Foad Farrag. 2020. "Ameliorative Effect of Graviola (Annona muricata) on Mono Sodium Glutamate-Induced Hepatic Injury in Rats: Antioxidant, Apoptotic, Anti-inflammatory, Lipogenesis Markers, and Histopathological Studies." Animals 10, no. 11: 1996.

Journal article
Published: 05 September 2020 in International Journal of Molecular Sciences
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Obesity is a worldwide life-threatening metabolic disorder, associated with various chronic diseases, including male infertility. Obesity was induced by high fat diet (HFD), and testis RNA was used for the transcriptome analysis using RNAseq via Illumina NovaSeq 6000 System and NovaSeq 6000 Kit. Gene expression level was estimated as FPKM (Fragments Per Kilobase of transcript per Million mapped reads). Differential expressed genes (DEGs) were annotated against gene ontology (GO) and KEGG databases. More than 63.66 million reads per sample were performed with 100 bp cutoff and 6 Gb sequencing depth. Results of this study revealed that 267 GO terms (245 biological processes (BP), 14 cellular components (CC), eight molecular functions (MF)), and 89 KEGG pathways were significantly enriched. Moreover, total numbers of 136 genes were differentially expressed (107 upregulated, 29 downregulated) with |FC| ≥ 2 and bh adjusted <0.05. Interesting DEGs were detected, including obesity and lipid metabolism-related genes, immune response-related genes, cytochrome P450 genes, including aromatase were upregulated, whereas genes related to male fertility and fertilization, cell adhesion, and olfactory receptors were downregulated. The combined expression pattern of the DEGs in obese animals indicated an increase in cholesterol metabolism. Furthermore, high aromatase activity enhances the testosterone turnover into estradiol and lowers the testosterone/estradiol (T/E) ratio, which ultimately reduces fertility. In addition, downregulation of cadherens junction components genes leads to the pre-mature release of sperm from Sertoli cells resulting in the reduction of fertility. Moreover, the downregulation of olfactory receptor genes reduces the chemotaxis capacity of sperms in tracking the oocyte for fertilization, which reduces male fertility. Furthermore, various obesity molecular markers were detected in our transcriptome. The results of this study will enhance our understanding of the molecular network of obesity development, development of obesity novel molecular diagnosis markers, molecular bases of obesity-induced infertility, and the development of anti-obesity drugs.

ACS Style

Ahmed El-Shehawi; Samir El-Shazly; Mohamed Ahmed; Mohamed Alkafafy; Samy Sayed; Samy Farouk; Saqer Alotaibi; Mona Elseehy. Transcriptome Analysis of Testis from HFD-Induced Obese Rats (Rattus norvigicus) Indicated Predisposition for Male Infertility. International Journal of Molecular Sciences 2020, 21, 6493 .

AMA Style

Ahmed El-Shehawi, Samir El-Shazly, Mohamed Ahmed, Mohamed Alkafafy, Samy Sayed, Samy Farouk, Saqer Alotaibi, Mona Elseehy. Transcriptome Analysis of Testis from HFD-Induced Obese Rats (Rattus norvigicus) Indicated Predisposition for Male Infertility. International Journal of Molecular Sciences. 2020; 21 (18):6493.

Chicago/Turabian Style

Ahmed El-Shehawi; Samir El-Shazly; Mohamed Ahmed; Mohamed Alkafafy; Samy Sayed; Samy Farouk; Saqer Alotaibi; Mona Elseehy. 2020. "Transcriptome Analysis of Testis from HFD-Induced Obese Rats (Rattus norvigicus) Indicated Predisposition for Male Infertility." International Journal of Molecular Sciences 21, no. 18: 6493.

Journal article
Published: 04 May 2020 in Plants
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Jojoba is one of the main two known plant source of natural liquid wax ester for use in various applications, including cosmetics, pharmaceuticals, and biofuel. Due to the lack of transcriptomic and genomic data on lipid biosynthesis and accumulation, molecular marker breeding has been used to improve jojoba oil production and quality. In the current study, the transcriptome of developing jojoba seeds was investigated using the Illunina NovaSeq 6000 system, 100 × 106 paired end reads, an average length of 100 bp, and a sequence depth of 12 Gb per sample. A total of 176,106 unigenes were detected with an average contig length of 201 bp. Gene Ontology (GO) showed that the detected unigenes were distributed in the three GO groups biological processes (BP, 5.53%), cellular component (CC, 6.06%), and molecular functions (MF, 5.88%) and distributed in 67 functional groups. The lipid biosynthesis pathway was established based on the expression of lipid biosynthesis genes, fatty acid (FA) biosynthesis, FA desaturation, FA elongation, fatty alcohol biosynthesis, triacylglycerol (TAG) biosynthesis, phospholipid metabolism, wax ester biosynthesis, and lipid transfer and storage genes. The detection of these categories of genes confirms the presence of an efficient lipid biosynthesis and accumulation system in developing jojoba seeds. The results of this study will significantly enhance the current understanding of wax ester biology in jojoba seeds and open new routes for the improvement of jojoba oil production and quality through biotechnology applications.

ACS Style

Saqer S. Alotaibi; Mona M. Elseehy; Bandar S. Aljuaid; Ahmed M. El-Shehawi. Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis. Plants 2020, 9, 588 .

AMA Style

Saqer S. Alotaibi, Mona M. Elseehy, Bandar S. Aljuaid, Ahmed M. El-Shehawi. Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis. Plants. 2020; 9 (5):588.

Chicago/Turabian Style

Saqer S. Alotaibi; Mona M. Elseehy; Bandar S. Aljuaid; Ahmed M. El-Shehawi. 2020. "Transcriptome Analysis of Jojoba (Simmondsia chinensis) during Seed Development and Liquid Wax Ester Biosynthesis." Plants 9, no. 5: 588.

Journal article
Published: 01 May 2020 in Cytology and Genetics
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DNA methylation is one epigenetic strategy for gene regulation in living organisms. In this study, the expression of soybean isoflavone synthase (GmIFS) transgene in T0 transgenic wheat plants was investigated at the RNA and the final product genestin level. T0 plants showed variations in the GmIFS expression. Methylation status of the exogenous promoters (35S or Oleocin (OL)) proximal sequence was investigated in T0 plants using bisulphite sequencing to disclose their methylation in parallel with the GmIFS level of expression. Results concluded that the high GmIFS expressers of T0 plants exhibited high methylation of exogenous promoter proximal sequences as well as low expression of DNA methyltransferases (Mets). Variation in GmIFS was associated with the level of methylation in the 35S or OL promoters. High expression of GmIFS was negatively correlated with methylation level of 35S and OL promoter proximal regions. In 35S promoter, methylation level of the CpG sites –56 and –88 is strongly linked to GmIFS expression and is involved in the regulation of GmIFS gene. In OL promoter, the CpG site could be involved in the regulation of the GmIFS. Wheat Met3 expression varied among T0 transgenic plants. Its expression profile could explain the regulation of GmIFS transgene by methylation.

ACS Style

Mona Mohamed Elseehy; Ahmed Mohamed El-Shehawi. Methylation of Exogenous Promoters Regulates Soybean Isoflavone Synthase (GmIFS) Transgene in T0 Transgenic Wheat (Triticum aestivum). Cytology and Genetics 2020, 54, 271 -282.

AMA Style

Mona Mohamed Elseehy, Ahmed Mohamed El-Shehawi. Methylation of Exogenous Promoters Regulates Soybean Isoflavone Synthase (GmIFS) Transgene in T0 Transgenic Wheat (Triticum aestivum). Cytology and Genetics. 2020; 54 (3):271-282.

Chicago/Turabian Style

Mona Mohamed Elseehy; Ahmed Mohamed El-Shehawi. 2020. "Methylation of Exogenous Promoters Regulates Soybean Isoflavone Synthase (GmIFS) Transgene in T0 Transgenic Wheat (Triticum aestivum)." Cytology and Genetics 54, no. 3: 271-282.

Journal article
Published: 08 April 2020 in Egyptian Journal of Biological Pest Control
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The endophytic fungus, Beauveria bassiana has been found to occur endophytically in hundreds of plant species tissues and has been inoculated for establishment in different plants. It has negative effect on piercing-sucking insects in grapevine plants. Thus, the present study aimed to detect the endophytic fungus, B. bassiana throughout ITS genes analysis in the grapevine plant, Vitis venifera cv. Taify and assess its potential for controlling the grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae). The obtained 5 isolates were DNA sequenced for ITS region. Data analysis showed that there were 2 different isolates (accession number: MN900613 for isolate Bb-Taif1 and MN900614 for isolate Bb-Taif2). The values of LC50 were 6.041 × 104, 3.199 × 105 spores/ml while those of LC90 were 6.13 × 106 and 7.474 × 107 spores/ml after 3 days of treatment by Bb-Taif1 and Bb-Taif2, respectively. These values showed that the virulence of Bb-Taif1 isolate was higher in its efficacy than the Bb-Taif2 isolate. Further investigations are needed to evaluate its efficacy on other piercing sucking or chewing insect pests throughout conidia spraying, soil inoculation, and/or root immersion for grapevine plants.

ACS Style

Samy Sayed; Ahmed El-Shehawi; Saad Al-Otaibi; Samir El-Shazly; Saqer Al-Otaibi; Reda Ibrahim; Mohammed Alorabi; Alaa Baazeem; Mona Elseehy. Isolation and efficacy of the endophytic fungus, Beauveria bassiana (Bals.) Vuillemin on grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae) under laboratory conditions. Egyptian Journal of Biological Pest Control 2020, 30, 1 -7.

AMA Style

Samy Sayed, Ahmed El-Shehawi, Saad Al-Otaibi, Samir El-Shazly, Saqer Al-Otaibi, Reda Ibrahim, Mohammed Alorabi, Alaa Baazeem, Mona Elseehy. Isolation and efficacy of the endophytic fungus, Beauveria bassiana (Bals.) Vuillemin on grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae) under laboratory conditions. Egyptian Journal of Biological Pest Control. 2020; 30 (1):1-7.

Chicago/Turabian Style

Samy Sayed; Ahmed El-Shehawi; Saad Al-Otaibi; Samir El-Shazly; Saqer Al-Otaibi; Reda Ibrahim; Mohammed Alorabi; Alaa Baazeem; Mona Elseehy. 2020. "Isolation and efficacy of the endophytic fungus, Beauveria bassiana (Bals.) Vuillemin on grapevine aphid, Aphis illinoisensis Shimer (Hemiptera: Aphididae) under laboratory conditions." Egyptian Journal of Biological Pest Control 30, no. 1: 1-7.

Journal article
Published: 02 January 2020 in Foods
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Obesity is a serious global problem that causes predisposition to numerous serious diseases. The current study aims to investigate the effect of ginger water on body weight and energy expenditure through modulation of mRNA expression of carbohydrate and lipid metabolism. A white colored liquid obtained during freeze-drying of fresh rhizomes of Zingiber officinal was collected and named ginger water. It was used to treat rats, then blood and tissue samples were collected from the liver and white adipose at the end of the experiment. The serum was prepared and used for biochemical assays, while tissue samples were used for RNA isolation and gene expression analysis via Reverse transcription polymerase chain reaction (RT-PCR). Results of High Performance Liquid Chromatography (HPLC) analysis of ginger water revealed the presence of chrysin and galangin at concentrations of 0.24 µg/mL and 0.53 µg/mL, respectively. Average body weight gain decreased significantly in groups that received ginger water. In addition, both total cholesterol and serum triacylglycerol were reduced in the groups that received ginger water. Furthermore, mRNA expression of Sterol regulatory element-binding protein 1 (SREBP-1c) in the liver and leptin in adipose tissues were downregulated, while those of adiponectin, hepatic carnitine palmitoyltransferase1 (CPT-1), acyl-coA oxidase (ACO), Glucose transporter 2 (GLUT-2), and pyruvate kinase (PK) were upregulated in ginger water-treated groups. These results clearly revealed the lowering body weight gain effect of ginger water, which most likely occurs at the transcriptional level of energy metabolizing proteins.

ACS Style

Samy Sayed; Mohamed Ahmed; Ahmed El-Shehawi; Mohamed Alkafafy; Saqer Al-Otaibi; Hanan El-Sawy; Samy Farouk; Samir El-Shazly. Ginger Water Reduces Body Weight Gain and Improves Energy Expenditure in Rats. Foods 2020, 9, 38 .

AMA Style

Samy Sayed, Mohamed Ahmed, Ahmed El-Shehawi, Mohamed Alkafafy, Saqer Al-Otaibi, Hanan El-Sawy, Samy Farouk, Samir El-Shazly. Ginger Water Reduces Body Weight Gain and Improves Energy Expenditure in Rats. Foods. 2020; 9 (1):38.

Chicago/Turabian Style

Samy Sayed; Mohamed Ahmed; Ahmed El-Shehawi; Mohamed Alkafafy; Saqer Al-Otaibi; Hanan El-Sawy; Samy Farouk; Samir El-Shazly. 2020. "Ginger Water Reduces Body Weight Gain and Improves Energy Expenditure in Rats." Foods 9, no. 1: 38.