The various social, political, environmental, and economical mega-trends together with the rapid rise of digitalization are shaping cities worldwide including the way that citizens and products move within the urban environment. New mobility services are popping up which are showing the path toward more on-demand and personalized mobility patterns. The transformation of the urban mobility environment though constitutes a great challenge for policy-makers, as little is known about the impact of these trends on the city’s current mobility environment while the assessment of the future state of a mobility system can be considered a quite complex process taken its multi-parametric and multi-dimensional nature. This paper aims to simplify this process by presenting a coherent multi-actor consequence analysis framework as a tool for assessing future mobility scenarios which was developed in the framework of the H2020 SPROUT. This paper presents in detail the main components of this framework together with the list of key performance indicators used for assessing the performance in terms of sustainability of future mobility scenarios as well as indicative results from its use by the city of Padua.