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Olga P. Gavrilova
All-Russian Institute of Plant Protection

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Journal article
Published: 13 March 2021 in Agrarian science
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Relevance. The scientific community and agricultural producers are looking for a solution to the problem of how to protect consumers from the effects of hazardous mycotoxins in agricultural products. One of the tools, which will be considered in this article, is a screening system that allows determining the quan-titative indicators of wheat grain infection by toxin-producing fungi in a short time. Methods. The method of quantitative PCR with the detection of fungal DNA and the method of enzyme immunoassay with the detection of Fusarium antigens were used to characterize the grain infection. Results. We established the lower critical limit of DNA content which is 3955·10-4 pg/ng and the lower critical limit of Fusarium antigens which is 596 U/g based on the analyzes of grain fungi contamination in model experiments. Grain should be subjected to mycotoxins analysis upon detection of these critical lim-its. All batches of grain can be used in production without additional analyzes if their values determined by analytical methods are below that critical points. The obtained values can be quantitative benchmarks corresponding to the standard visual analysis of grain contamination currently described in GOST 31646-2012

ACS Style

Yu. S. Lebedin; A. S. Orina; O. P. Gavrilova; T. Y. Gagkaeva; V. N. Maigurova; P. A. Petukhov. Application of analytical methods to identify critical limits of grain infection by Fusarium fungi. Agrarian science 2021, 344, 92-97 .

AMA Style

Yu. S. Lebedin, A. S. Orina, O. P. Gavrilova, T. Y. Gagkaeva, V. N. Maigurova, P. A. Petukhov. Application of analytical methods to identify critical limits of grain infection by Fusarium fungi. Agrarian science. 2021; 344 (1):92-97.

Chicago/Turabian Style

Yu. S. Lebedin; A. S. Orina; O. P. Gavrilova; T. Y. Gagkaeva; V. N. Maigurova; P. A. Petukhov. 2021. "Application of analytical methods to identify critical limits of grain infection by Fusarium fungi." Agrarian science 344, no. 1: 92-97.

Conference paper
Published: 28 June 2020 in Proceedings on applied botany, genetics and breeding
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Background. The requirement for high-quality oat grain for food production leads to the need for studying the resistance of this cereal crop to harmful diseases and for the development of new productive cultivars.Materials and methods. A comparative characterization was performed among the breeding lines of naked oats from Nemchinovka Federal Research Center according to the parameters of microbiological purity of grain. The mycological method was applied for the analysis of infection in the oat grain, and fungal species composition was identified. The DNA amount of toxin-producing species from genus Fusarium Link in the grain was detected using the real-time PCR, and the amount of mycotoxins was measured by the enzyme immunoassay.Results. The fungi of Alternaria Nees, Fusarium, Cladosporium Link, Cochliobolus Drechsler, Epicoccum Link were associated with oat grain. Uniquely high infection of the grain by F. langsethiae Torp & Nirenberg (14%) was found under the natural conditions of the Central Region of Russia. The differences revealed among the analyzed breeding lines and cultivars in the DNA amount of F. langsethiae and F. sporotrichioides Sherb. were from 1.3×10-4 to 7.2×10-3 pg/ng in the total DNA, and from 5 to 1230 μg/kg in the content of T-2/ HT-2 toxins.Conclusion. All naked oat breeding lines were relatively resistant to Fusarium infection and proved the best as regards the mycotoxin contamination of the grain against the reference hulled cultivar ‘Yakov’. In the competitive variety trials, three lines – 66h2618, 54h2476 and 70h2613 – were characterized as relatively resistant to Fusarium disease, compared with the naked oat reference ‘Vyatsky golozerny’.

ACS Style

O. P. Gavrilova; T. Yu. Gagkaeva; A. S. Orina; A. S. Markova; A. D. Kabashov; I. G. Loskutov. Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province. Proceedings on applied botany, genetics and breeding 2020, 181, 134-144 .

AMA Style

O. P. Gavrilova, T. Yu. Gagkaeva, A. S. Orina, A. S. Markova, A. D. Kabashov, I. G. Loskutov. Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province. Proceedings on applied botany, genetics and breeding. 2020; 181 (2):134-144.

Chicago/Turabian Style

O. P. Gavrilova; T. Yu. Gagkaeva; A. S. Orina; A. S. Markova; A. D. Kabashov; I. G. Loskutov. 2020. "Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province." Proceedings on applied botany, genetics and breeding 181, no. 2: 134-144.

Journal article
Published: 22 June 2020 in Agricultural and Food Science
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This study aimed to assess the contamination of Fabaceae plants with fungi and mycotoxins, which have a negative effect on the health of consumers of plant products. Quantitative PCR was used to analyse the DNA of Alternaria, Cladosporium and Fusarium fungi in 69 plant samples harvested from four Russian regions in 2015. Additionally, mycotoxins were analysed using ELISA. Cladosporium fungi DNA was found in all the analysed samples; the occurrence of Alternaria and Fusarium DNA was 84% and 51%, respectively. Mycotoxin alternariol was detected in 100% and emodin in 90% of the samples. The occurrence of deoxynivalenol, diacetoxyscirpenol and T-2/HT-2 mycotoxins was 32%, 42% and 41%, respectively. Plant species had no significant effect on the Alternaria, Cladosporium and Fusarium DNA content of the samples; however, the alternariol, emodin, deoxynivalenol and diacetoxyscirpenol contents differed significantly between plant species. The geographical origin had a statistically significant effect on the Alternaria and Fusarium DNA contents, likely due to differences in weather conditions.

ACS Style

Aleksandra Orina; Olga P. Gavrilova; Tatiana Gagkaeva; Aleksey Burkin; Galina Kononenko. The contamination of Fabaceae plants with fungi and mycotoxins. Agricultural and Food Science 2020, 29, 265–275 -265–275.

AMA Style

Aleksandra Orina, Olga P. Gavrilova, Tatiana Gagkaeva, Aleksey Burkin, Galina Kononenko. The contamination of Fabaceae plants with fungi and mycotoxins. Agricultural and Food Science. 2020; 29 (3):265–275-265–275.

Chicago/Turabian Style

Aleksandra Orina; Olga P. Gavrilova; Tatiana Gagkaeva; Aleksey Burkin; Galina Kononenko. 2020. "The contamination of Fabaceae plants with fungi and mycotoxins." Agricultural and Food Science 29, no. 3: 265–275-265–275.

Full paper
Published: 18 May 2020 in Chemistry & Biodiversity
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The biological characterization of Microdochium majus, M. nivale, and M. seminicola strains with wide geographical origins showed the diversity of their pathogenic properties and metabolite compounds, allowing them to exist in their habitats. Significant differences in the ability of Microdochium fungi to cause lesions on wheat and oat leaves were found. The intensity of symptoms depended on the species and substrate origin of the strains. On average M. seminicola strains were able to cause less leaf necrosis than M. majus and M nivale. The volatile organic compound (VOC) profile of Microdochium fungi included 29 putative fungal metabolites. The spectrum of the identified VOCs in M. seminicola strains was much richer than that in M. majus and M. nivale strains. In addition, the strains of M. seminicola emitted at least six sesquiterpenes. Mycotoxin analysis by HPLC‐MS/MS revealed that the analysed Microdochium strains did not produce any toxic metabolites typically produced by filamentous fungi.

ACS Style

Olga P. Gavrilova; Aleksandra S. Orina; Elizaveta D. Kessenikh; Lyudmila K. Gustyleva; Elena I. Savelieva; Nadezhda N. Gogina; Tatiana Yu. Gagkaeva. Diversity of Physiological and Biochemical Characters of Microdochium Fungi. Chemistry & Biodiversity 2020, 17, 1 .

AMA Style

Olga P. Gavrilova, Aleksandra S. Orina, Elizaveta D. Kessenikh, Lyudmila K. Gustyleva, Elena I. Savelieva, Nadezhda N. Gogina, Tatiana Yu. Gagkaeva. Diversity of Physiological and Biochemical Characters of Microdochium Fungi. Chemistry & Biodiversity. 2020; 17 (8):1.

Chicago/Turabian Style

Olga P. Gavrilova; Aleksandra S. Orina; Elizaveta D. Kessenikh; Lyudmila K. Gustyleva; Elena I. Savelieva; Nadezhda N. Gogina; Tatiana Yu. Gagkaeva. 2020. "Diversity of Physiological and Biochemical Characters of Microdochium Fungi." Chemistry & Biodiversity 17, no. 8: 1.

Journal article
Published: 28 February 2020 in Microorganisms
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In total, 46 Microdochium strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as M. nivale and M. majus, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as M. seminicola, which, according to our findings, is the prevalent Microdochium species in this territory. This is the first record of M. seminicola in Russia. Attempts were made to distinguish between Microdochium species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of Microdochium. The predominance of three-septate macroconidia in M. majus was higher than that in M. nivale and typically exceeded 60% occurrence. Most M. majus and M. nivale strains formed walled protoperithecia on wheat stems. Only three strains of M. majus and one strain each of M. nivale and M. seminicola produced mature perithecia. The growth rate of M. seminicola strains was significantly lower on agar media at 5–25 °C than those of M. majus and M. nivale strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three Microdochium species did not produce any toxic metabolites.

ACS Style

Tatiana Yu. Gagkaeva; Aleksandra S. Orina; Olga P. Gavrilova; Nadezhda N. Gogina. Evidence of Microdochium Fungi Associated with Cereal Grains in Russia. Microorganisms 2020, 8, 340 .

AMA Style

Tatiana Yu. Gagkaeva, Aleksandra S. Orina, Olga P. Gavrilova, Nadezhda N. Gogina. Evidence of Microdochium Fungi Associated with Cereal Grains in Russia. Microorganisms. 2020; 8 (3):340.

Chicago/Turabian Style

Tatiana Yu. Gagkaeva; Aleksandra S. Orina; Olga P. Gavrilova; Nadezhda N. Gogina. 2020. "Evidence of Microdochium Fungi Associated with Cereal Grains in Russia." Microorganisms 8, no. 3: 340.

Journal article
Published: 05 May 2019 in Toxins
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Wheat grains collected in three regions of Russia—Volga, Ural, and West Siberia—were analyzed for triangulation of methods in analysis of toxigenic Fusarium species. The presence of fungi and quantitative content of their biomass were detected by using various analytical methods, including a mycological and immunochemical methods, and quantitative PCR. Additionally, an enzyme-linked immunosorbent assay and high-performance liquid chromatography with tandem mass spectrometry were applied for determination of mycotoxins. Regional differences were found regarding the contamination of wheat grain by Fusarium fungi and their toxins. The most important observation was the detection of F. graminearum in the Ural and West Siberian regions, where this pathogen had not been found previously. A maximum damaged grains by F. graminearum and F. sporotrichioides was found in the grain samples from West Siberia. The DNA of F. graminearum was detected in 19.2% and DNA of F. sporotrichioides was found in 84.1% of the analyzed grain samples. The amount of Fusarium antigens in the grain samples from the West Siberian region was 7–8 times higher than in the grain samples from the other two regions. Significant contamination of the grain with deoxynivalenol and T-2/HT-2 toxins (maximum contents were 2239 ppb and 199 ppb, respectively) was detected in the West Siberian region.

ACS Style

Tatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins 2019, 11, 252 .

AMA Style

Tatiana Gagkaeva, Olga Gavrilova, Aleksandra Orina, Yuri Lebedin, Ilya Shanin, Pavel Petukhov, Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins. 2019; 11 (5):252.

Chicago/Turabian Style

Tatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. 2019. "Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia." Toxins 11, no. 5: 252.

Note
Published: 01 March 2019 in Plant Disease
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T. Y. Gagkaeva; Olga P. Gavrilova; A. S. Orina. First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia. Plant Disease 2019, 103, 588 -588.

AMA Style

T. Y. Gagkaeva, Olga P. Gavrilova, A. S. Orina. First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia. Plant Disease. 2019; 103 (3):588-588.

Chicago/Turabian Style

T. Y. Gagkaeva; Olga P. Gavrilova; A. S. Orina. 2019. "First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia." Plant Disease 103, no. 3: 588-588.

Journal article
Published: 29 November 2018 in Pathogens
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Fusarium avenaceum and closely related species are common fungi on various plants, cultivated in different climatic regions. The aim of this study was to determine the taxonomic affiliations of the F. avenaceum, Fusarium arthrosporioides, and Fusarium anguioides strains by using morphological, physiological and molecular-genetic approaches. Twenty-six single-spored morphologically identified strains, which were mainly from cereals, were investigated in order to find out, if they belong to a separate species. Pathogenicity of strains to wheat seedlings and ISSR (Inter Simple Sequence Repeats) fingerprint and beta-tubulin DNA sequence patterns were analyzed. According to phylogenetic analyses, the strains could be divided into two big groups consisting of mostly F. avenaceum or F. anguioides strains. F. arthrosporioides was not detected as a separate species by the sum of the characters. F. anguioides was characterized as a separate species, which could be identified by morphological and molecular data. High genetic diversity of the F. avenaceum species complex was revealed. One F. anguioides strain (rudbeckia, Vladivostok, Russia), had an identical beta-tubulin sequence with two previously sequenced strains of Fusarium tricinctum species complex, which were isolated from dicotyledonous plants in Asia.

ACS Style

Tapani Yli-Mattila; Taha Hussien; Olga Gavrilova; Tatiana Gagkaeva. Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains. Pathogens 2018, 7, 94 .

AMA Style

Tapani Yli-Mattila, Taha Hussien, Olga Gavrilova, Tatiana Gagkaeva. Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains. Pathogens. 2018; 7 (4):94.

Chicago/Turabian Style

Tapani Yli-Mattila; Taha Hussien; Olga Gavrilova; Tatiana Gagkaeva. 2018. "Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains." Pathogens 7, no. 4: 94.

Journal article
Published: 27 September 2018 in Vavilov Journal of Genetics and Breeding
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In this study, naturally and artificially inoculated winter wheat varieties were studied with respect to their productivity and resistance to Fusarium head blight (FHB). We used the following set of disease assessment parameters: the percentage of visually and latent Fusarium-damaged grains (FDG); the DNA content of Fusarium fungi; the productivity of inoculated plants compared with non-inoculated plants; and the amount of mycotoxins in the grain. In case of naturally infected grains, the average FDG was found to be about 6.1 % (range of 0–15 %). The amount of DNA of Fusarium graminearum was found to be in the range of (1.1–42.7) × 10–5 ng/ng wheat DNA. The mycotoxin deoxynivalenol (DON) was detected in 15 samples of grain from plants that were grown under natural infection. The maximum DON amount was found to be 420 μg/kg. Fumonisin B1 (FB1) was not be detected in naturally infected grain. In case of artificially inoculated plants, the average FDG was found to be 25.8 % (2–54 %). The amount of F. graminearum DNA was found to be significantly higher (4.24– 49.8) × 10–3 ng/ng than it was detected in grain of non-inoculated plants. The wheat varieties inoculated with F. graminearum contained DON in high amounts from 20255 to 79245 μg/kg. Furthermore, a significant amount of FB1 was detected in all wheat varieties in the range of 980–20326 μg/kg. Among the analysed wheat varieties, Adel was characterized to be the most resistant to fungal infection as well as to the contamination by mycotoxins. Antonina, Lebed and Pamyat varieties were classified more relatively resistant than that of other varieties, and Utrish variety was found to be the most susceptible to FHB. The similar resistance of wheat varieties against F. graminearum and F. verticillioides infection was recorded, and the interactions between the fungi during the colonization of grain were shown.

ACS Style

T. Yu. Gagkaeva; A. S. Orina; O. P. Gavrilova; I. B. Ablova; L. A. Bespalova. Characterization of resistance of winter wheat varieties to Fusarium head blight. Vavilov Journal of Genetics and Breeding 2018, 22, 685 -692.

AMA Style

T. Yu. Gagkaeva, A. S. Orina, O. P. Gavrilova, I. B. Ablova, L. A. Bespalova. Characterization of resistance of winter wheat varieties to Fusarium head blight. Vavilov Journal of Genetics and Breeding. 2018; 22 (6):685-692.

Chicago/Turabian Style

T. Yu. Gagkaeva; A. S. Orina; O. P. Gavrilova; I. B. Ablova; L. A. Bespalova. 2018. "Characterization of resistance of winter wheat varieties to Fusarium head blight." Vavilov Journal of Genetics and Breeding 22, no. 6: 685-692.

Journal article
Published: 01 January 2018 in PLANT PROTECTION NEWS
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A.S. Orina; O.P. Gavrilova; T.Yu. Gagkaeva. Analysis of contamination of annual and perennial Leguminosae grasses with fungi using quantitative PCR. PLANT PROTECTION NEWS 2018, 1 .

AMA Style

A.S. Orina, O.P. Gavrilova, T.Yu. Gagkaeva. Analysis of contamination of annual and perennial Leguminosae grasses with fungi using quantitative PCR. PLANT PROTECTION NEWS. 2018; (2(96)):1.

Chicago/Turabian Style

A.S. Orina; O.P. Gavrilova; T.Yu. Gagkaeva. 2018. "Analysis of contamination of annual and perennial Leguminosae grasses with fungi using quantitative PCR." PLANT PROTECTION NEWS , no. 2(96): 1.

Journal article
Published: 01 January 2018 in Russian Journal of Genetics: Applied Research
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Sixty-six genotypes of 19 Avena species of different ploidy from VIR collection are analyzed for resistance to Fusarium head blight. The plants were infected in the field with Fusarium culmorum strains. For the first time in the world a representative set of the wild Avena species is assessed. The DNA content of trichothecene-producing Fusarium fungi was identified by a quantitative polymerase chain reaction (qPCR) method. The amount of mycotoxins in the grain was determined by an enzyme-linked immunosorbent assay (ELISA) method. The smallest amounts of Fusarium DNA and deoxynivalenol (DON) were detected in seven genotypes of hexaploid species: A. byzantina, A. sterilis, A. sativa and A. fatua and one diploid species A. wiestii. Statistically significant connections were demonstrated between the morphological traits of the genotypes, the Fusarium DNA content and amount of DON.

ACS Style

T. Yu. Gagkaeva; O. P. Gavrilova; A. S. Orina; E. V. Blinova; I. G. Loskutov. Diversity of Avena Species by Morphological Traits and Resistance to Fusarium Head Blight. Russian Journal of Genetics: Applied Research 2018, 8, 44 -51.

AMA Style

T. Yu. Gagkaeva, O. P. Gavrilova, A. S. Orina, E. V. Blinova, I. G. Loskutov. Diversity of Avena Species by Morphological Traits and Resistance to Fusarium Head Blight. Russian Journal of Genetics: Applied Research. 2018; 8 (1):44-51.

Chicago/Turabian Style

T. Yu. Gagkaeva; O. P. Gavrilova; A. S. Orina; E. V. Blinova; I. G. Loskutov. 2018. "Diversity of Avena Species by Morphological Traits and Resistance to Fusarium Head Blight." Russian Journal of Genetics: Applied Research 8, no. 1: 44-51.

Journal article
Published: 01 January 2018 in Microbiology Independent Research Journal
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ACS Style

Aleksandra S. Orina; Olga P. Gavrilova; Tatiana Yu. Gagkaeva. Adaptation of the quantitative PCR method for the detection of the main representatives of cereal grain mycobiota. Microbiology Independent Research Journal 2018, 5, 1 .

AMA Style

Aleksandra S. Orina, Olga P. Gavrilova, Tatiana Yu. Gagkaeva. Adaptation of the quantitative PCR method for the detection of the main representatives of cereal grain mycobiota. Microbiology Independent Research Journal. 2018; 5 (1):1.

Chicago/Turabian Style

Aleksandra S. Orina; Olga P. Gavrilova; Tatiana Yu. Gagkaeva. 2018. "Adaptation of the quantitative PCR method for the detection of the main representatives of cereal grain mycobiota." Microbiology Independent Research Journal 5, no. 1: 1.

Journal article
Published: 01 December 2017 in The Crop Journal
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ACS Style

T.Yu. Gagkaeva; O.P. Gavrilova; A.S. Orina; E.V. Blinova; I.G. Loskutov. Response of wild Avena species to fungal infection of grain. The Crop Journal 2017, 5, 499 -508.

AMA Style

T.Yu. Gagkaeva, O.P. Gavrilova, A.S. Orina, E.V. Blinova, I.G. Loskutov. Response of wild Avena species to fungal infection of grain. The Crop Journal. 2017; 5 (6):499-508.

Chicago/Turabian Style

T.Yu. Gagkaeva; O.P. Gavrilova; A.S. Orina; E.V. Blinova; I.G. Loskutov. 2017. "Response of wild Avena species to fungal infection of grain." The Crop Journal 5, no. 6: 499-508.

Journal article
Published: 01 October 2017 in Sel'skokhozyaistvennaya Biologiya
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ACS Style

A.S. Orina; O.P. Gavrilova; T.Yu. Gagkaeva; I.G. Loskutov. SYMBIOTIC RELATIONSHIPS BETWEEN AGGRESSIVE Fusarium AND Alternaria FUNGI COLONIZING OAT GRAIN. Sel'skokhozyaistvennaya Biologiya 2017, 52, 986 -994.

AMA Style

A.S. Orina, O.P. Gavrilova, T.Yu. Gagkaeva, I.G. Loskutov. SYMBIOTIC RELATIONSHIPS BETWEEN AGGRESSIVE Fusarium AND Alternaria FUNGI COLONIZING OAT GRAIN. Sel'skokhozyaistvennaya Biologiya. 2017; 52 (5):986-994.

Chicago/Turabian Style

A.S. Orina; O.P. Gavrilova; T.Yu. Gagkaeva; I.G. Loskutov. 2017. "SYMBIOTIC RELATIONSHIPS BETWEEN AGGRESSIVE Fusarium AND Alternaria FUNGI COLONIZING OAT GRAIN." Sel'skokhozyaistvennaya Biologiya 52, no. 5: 986-994.

Journal article
Published: 03 May 2017 in Russian Journal of Genetics: Applied Research
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A comprehensive assessment of the eco-geographical and intraspecific diversity of oats helps to determine the characters, based on which it is possible to select genotypes that may be used as genetic sources for breeding. The study aims to analyse the relationship of the agronomic traits of oat genotypes from the collection of N.I. Vavilov Institute of Plant Genetic Resources (VIR) with resistance to the Fusarium disease. The agronomic characters and disease resistance of 340 genotypes of hulled and naked oats of the cultivated species such Avena sativa L., A. byzantina C. Koch, A. abyssinica Hoch, and A. strigosa Schreb. from the different geographical origins were tested under field and laboratory conditions in 2007–2009 and 2014. The artificial inoculation with Fusarium sporotrichioides Sherb. was used to evaluate the oat genotypes based on grain infection and mycotoxin accumulation. An integral analysis demonstrated that an increased duration of the second half of the growth period, the presence of resistance to lodging and leaf pathogen infection, decreased plant height, and increased length of the panicle contributed to the enhancement of Fusarium damaged grains. It was established that A. strigosa and A. sativa were less susceptible to Fusarium disease than A. byzantina and A. abyssinica. Oats of naked form were more resistant to Fusarium disease than the hulled forms. The comparison of the evaluation results has demonstrated that landraces with the unilateral panicle and dark-coloured glumes were more resistant to Fusarium disease than the breeding varieties. Consideration of the geographical origin revealed a higher degree of resistance to the Fusarium disease in the hulled landraces from Russia, Belarus, United States, and China. On the basis of the complex of studied characteristics, oat genotypes with high productivity and resistance to Fusarium disease were revealed.

ACS Style

I. G. Loskutov; E. V. Blinova; Olga P. Gavrilova; T. Yu. Gagkaeva. The valuable characteristics and resistance to Fusarium disease of oat genotypes. Russian Journal of Genetics: Applied Research 2017, 7, 290 -298.

AMA Style

I. G. Loskutov, E. V. Blinova, Olga P. Gavrilova, T. Yu. Gagkaeva. The valuable characteristics and resistance to Fusarium disease of oat genotypes. Russian Journal of Genetics: Applied Research. 2017; 7 (3):290-298.

Chicago/Turabian Style

I. G. Loskutov; E. V. Blinova; Olga P. Gavrilova; T. Yu. Gagkaeva. 2017. "The valuable characteristics and resistance to Fusarium disease of oat genotypes." Russian Journal of Genetics: Applied Research 7, no. 3: 290-298.

Journal article
Published: 01 April 2017 in Sel'skokhozyaistvennaya Biologiya
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A.A. Burkin; G.P. Kononenko; O.P. Gavrilova; T.Yu. Gagkaeva. MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA. Sel'skokhozyaistvennaya Biologiya 2017, 52, 409 -417.

AMA Style

A.A. Burkin, G.P. Kononenko, O.P. Gavrilova, T.Yu. Gagkaeva. MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA. Sel'skokhozyaistvennaya Biologiya. 2017; 52 (2):409-417.

Chicago/Turabian Style

A.A. Burkin; G.P. Kononenko; O.P. Gavrilova; T.Yu. Gagkaeva. 2017. "MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA." Sel'skokhozyaistvennaya Biologiya 52, no. 2: 409-417.

Journal article
Published: 31 March 2017 in Microorganisms
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Analysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the intergenic spacer (IGS) region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported.

ACS Style

Olga Gavrilova; Anna Skritnika; Tatiana Gagkaeva. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms 2017, 5, 14 .

AMA Style

Olga Gavrilova, Anna Skritnika, Tatiana Gagkaeva. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms. 2017; 5 (2):14.

Chicago/Turabian Style

Olga Gavrilova; Anna Skritnika; Tatiana Gagkaeva. 2017. "Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae." Microorganisms 5, no. 2: 14.

Journal article
Published: 15 March 2017 in Ecological genetics
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Sixty six genotypes belong to wild and cultivated Avena species from the VIR collection were evaluated for infection of grain by Fusarium fungi and mycotoxins in accumulation. Among genotypes 13.6, 28.8, and 57.6% were diploid, tetraploid, and hexaploid oats, respectively. The aim of this study was to recognize the interrelationship between the wild species of the genus Avena not subjected to formal breeding programs, and Fusarium fungi, which have been reported as predominant seed borne pathogens. The real-time PCR was used to simultaneously detect and quantify fungi in grain of Avena genotypes. Mycotoxin analysis was performed by ELISA. The average amount of Fusarium DNA and deoxynivalenol (DON) in groups of the tetraploid oats were higher than they were found in the groups of di- and hexaploid species. It was determining the strong correlation between the presence of Tri-Fusarium DNA and DON content (p Keywords Fusarium, Avena species, ploidy, fungal DNA, Fusarium, mycotoxins, real-time PCR, ELISA.

ACS Style

Taiana Yu Gagkaeva; Olga P Gavrilova; Alexandra A Orina; Elena V Blinova; Igor G Loskutov. Diversity of the species of genus Avena revealed by morphological characters and resistance to Fusarium infection of grain. Ecological genetics 2017, 15, 20 .

AMA Style

Taiana Yu Gagkaeva, Olga P Gavrilova, Alexandra A Orina, Elena V Blinova, Igor G Loskutov. Diversity of the species of genus Avena revealed by morphological characters and resistance to Fusarium infection of grain. Ecological genetics. 2017; 15 (1):20.

Chicago/Turabian Style

Taiana Yu Gagkaeva; Olga P Gavrilova; Alexandra A Orina; Elena V Blinova; Igor G Loskutov. 2017. "Diversity of the species of genus Avena revealed by morphological characters and resistance to Fusarium infection of grain." Ecological genetics 15, no. 1: 20.

Journal article
Published: 04 July 2016 in Chemistry & Biodiversity
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The compositions of volatile organic compounds (VOCs) emitted by Fusarium fungi (F. langsethiae, F. sibiricum, F. poae, and F. sporotrichioides) grown on two nutritive substrates: potato sucrose agar (PSA) and autoclaved wheat kernels (WK) were investigated. The culturing of fungi and study of their VOC emissions were performed in chromatographic vials at room temperature (23 – 24 °C) and the VOCs were sampled by solid-phase microextraction on a 85 μm Carboxen/Polydimethylsiloxane fiber. GC/MS was performed using a 60-m HP-5 capillary column. Components of the VOC mixture were identified by electron impact mass spectra and chromatographic retention indices (RIs).The most abundant components of the VOC mixture emitted by Fusarium fungi are ethanol, ethyl acetate, isobutanol, 3-methylbutan-1-ol, 2-methylbutan-1-ol, ethyl 3-methylbutanoate, terpenes with M 136, sesquiterpenes with M 204 (a total of about 25), and trichodiene. It was found that the strains grown on PSA emit a wider spectrum and larger amount of VOCs compared with those grown on wheat kernels. F. langsethiae strain is the most active VOC producer on both substrates. The use of SPME and GC/MS also offers the potential for differentiation of fungal species and strains.This article is protected by copyright. All rights reserved.

ACS Style

Elena I. Savelieva; Liudmila K. Gustyleva; Elizaveta Kessenikh; Natalya S. Khlebnikova; John Leffingwell; Olga P. Gavrilova; Tatiana Yu. Gagkaeva; Ludmila K. Gustyleva. Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates. Chemistry & Biodiversity 2016, 13, 891 -903.

AMA Style

Elena I. Savelieva, Liudmila K. Gustyleva, Elizaveta Kessenikh, Natalya S. Khlebnikova, John Leffingwell, Olga P. Gavrilova, Tatiana Yu. Gagkaeva, Ludmila K. Gustyleva. Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates. Chemistry & Biodiversity. 2016; 13 (7):891-903.

Chicago/Turabian Style

Elena I. Savelieva; Liudmila K. Gustyleva; Elizaveta Kessenikh; Natalya S. Khlebnikova; John Leffingwell; Olga P. Gavrilova; Tatiana Yu. Gagkaeva; Ludmila K. Gustyleva. 2016. "Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates." Chemistry & Biodiversity 13, no. 7: 891-903.

Journal article
Published: 01 February 2016 in Sel'skokhozyaistvennaya Biologiya
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ACS Style

O.P. Gavrilova; Ph.B. Gannibal; T.Yu. Gagkaeva. Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY. Sel'skokhozyaistvennaya Biologiya 2016, 51, 111 -118.

AMA Style

O.P. Gavrilova, Ph.B. Gannibal, T.Yu. Gagkaeva. Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY. Sel'skokhozyaistvennaya Biologiya. 2016; 51 (1):111-118.

Chicago/Turabian Style

O.P. Gavrilova; Ph.B. Gannibal; T.Yu. Gagkaeva. 2016. "Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY." Sel'skokhozyaistvennaya Biologiya 51, no. 1: 111-118.