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Relevance. The scientific community and agricultural producers are looking for a solution to the problem of how to protect consumers from the effects of hazardous mycotoxins in agricultural products. One of the tools, which will be considered in this article, is a screening system that allows determining the quan-titative indicators of wheat grain infection by toxin-producing fungi in a short time. Methods. The method of quantitative PCR with the detection of fungal DNA and the method of enzyme immunoassay with the detection of Fusarium antigens were used to characterize the grain infection. Results. We established the lower critical limit of DNA content which is 3955·10-4 pg/ng and the lower critical limit of Fusarium antigens which is 596 U/g based on the analyzes of grain fungi contamination in model experiments. Grain should be subjected to mycotoxins analysis upon detection of these critical lim-its. All batches of grain can be used in production without additional analyzes if their values determined by analytical methods are below that critical points. The obtained values can be quantitative benchmarks corresponding to the standard visual analysis of grain contamination currently described in GOST 31646-2012
Yu. S. Lebedin; A. S. Orina; O. P. Gavrilova; T. Y. Gagkaeva; V. N. Maigurova; P. A. Petukhov. Application of analytical methods to identify critical limits of grain infection by Fusarium fungi. Agrarian science 2021, 344, 92-97 .
AMA StyleYu. S. Lebedin, A. S. Orina, O. P. Gavrilova, T. Y. Gagkaeva, V. N. Maigurova, P. A. Petukhov. Application of analytical methods to identify critical limits of grain infection by Fusarium fungi. Agrarian science. 2021; 344 (1):92-97.
Chicago/Turabian StyleYu. S. Lebedin; A. S. Orina; O. P. Gavrilova; T. Y. Gagkaeva; V. N. Maigurova; P. A. Petukhov. 2021. "Application of analytical methods to identify critical limits of grain infection by Fusarium fungi." Agrarian science 344, no. 1: 92-97.
Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. Previously (Geiser et al. 2013; Phytopathology 103:400-408. 2013), the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani Species Complex (FSSC). Subsequently, this concept was challenged by one research group (Lombard et al. 2015 Studies in Mycology 80: 189-245) who proposed dividing Fusarium into seven genera, including the FSSC as the genus Neocosmospora, with subsequent justification based on claims that the Geiser et al. (2013) concept of Fusarium is polyphyletic (Sandoval-Denis et al. 2018; Persoonia 41:109-129). Here we test this claim, and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species recently described as Neocosmospora were recombined in Fusarium, and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium, as it remains the best scientific, nomenclatural and practical taxonomic option available.
David M Geiser; Abdullah Al-Hatmi; Takayuki Aoki; Tsutomu Arie; Virgilio Balmas; Irene Barnes; Gary C Bergstrom; M.K. K. Bhattacharyya; Cheryl L. Blomquist; Robert Bowden; Balázs Brankovics; Daren W. Brown; Lester William Burgess; Kathryn Bushley; Mark Busman; José F. Cano-Lira; Joseph D. Carrillo; Hao-Xun Chang; Chi-Yu Chen; Wanquan Chen; Martin I. Chilvers; Sofia Noemi Chulze; Jeffrey J. Coleman; Christina A. Cuomo; Z. Wilhelm De Beer; G. Sybren De Hoog; Johanna Del Castillo-Múnera; Emerson Del Ponte; Javier Diéguez-Uribeondo; Antonio Di Pietro; Vérnonique Edel-Hermann; Wade H Elmer; Lynn Epstein; Akif Eskalen; Maria Carmela Esposto; Kathryne L. Everts; Sylvia P. Fernández-Pavía; Gilvan Ferreira Da Silva; Nora A Foroud; Gerda Fourie; Rasmus J.N. Frandsen; Stanley Freeman; Michael Freitag; Omer Frenkel; Kevin K Fuller; Tatiana Gagkaeva; Donald Max Gardiner; Anthony E. Glenn; Scott Gold; Tom Gordon; Nancy F. Gregory; Marieka Gryzenhout; Josep Guarro; Beth Gugino; Santiago Gutiérrez; Kim Hammond-Kosack; Linda J. Harris; Mónika Homa; Cheng-Fang Hong; László Hornok; Jenn-Wen Huang; Macit Ilkit; Adriaana Jacobs; Karin Jacobs; Cong Jiang; Maria Del Mar Jimenez-Gasco; Seogchan Kang; Matthew T Kasson; Kemal Kazan; John Carlyle Kennell; Hyeseon Kim; Harold Corby Kistler; Gretchen A. Kuldau; Tomasz Kulik; Oliver Kurzai; Imane Laraba; Matthew H. Laurence; Theresa Yun Lee; Yin-Won Lee; Yong-Hwan Lee; John F. Leslie; Edward C.Y. Liew; Lily W. Lofton; Antonio Logrieco; Manuel Sánchez López-Berges; Alicia Graciela Luque; Erik Lysøe; Li-Jun Ma; Robert E Marra; Frank N. Martin; Sara Ruth May; Susan McCormick; Chyanna T McGee; Jacques F. Meis; Quirico Migheli; Nik Mohd Izham Mohamed Nor; Michel Monod; Antonio Moretti; Diane Mostert; Giuseppina. Mulé; Françoise Munaut; Gary P Munkvold; Paul Nicholson; Marcio Nucci; Kerry O'Donnell; Matias Pasquali; Ludwig H. Pfenning; Anna Prigitano; Robert Proctor; Stéphane Ranque; Stephen Rehner; Martijn Rep; Gerardo Rodríguez-Alvarado; Lindy J Rose; Mitchell George Roth; Carmen Ruiz-Roldán; Amgad A Saleh; Baharuddin Salleh; Hyunkyu Sang; Mercedes Scandiani; Jonathan Scauflaire; David Schmale; Dylan Pg Short; Adnan Šišić; Jason Smith; Christopher W. Smyth; Hokyoung Son; Ellie Spahr; Jason E Stajich; Emma Steenkamp; Christian Steinberg; Rajagopal Subramaniam; Haruhisa Suga; Brett Anthony Summerell; Antonella Susca; Cassandra Lynn Swett; Christopher Toomajian; Terry Jarianna Torres-Cruz; Anna Maria Tortorano; Martin Urban; Lisa J. Vaillancourt; Gary E Vallad; Theo Van Der Lee; Dan Vanderpool; Anne D. Van Diepeningen; Martha Vaughan; Eduard Venter; Marcele Vermeulen; Paul E. Verweij; Altus Viljoen; Cees Waalwijk; Emma C. Wallace; Grit Walther; Jie Wang; Todd Ward; Brian Wickes; Nathan P. Wiederhold; Michael J. Wingfield; Ana K.M. Wood; Jin-Rong Xu; X. B. Yang; Tapani Yli-Matilla; Sung-Hwan Yun; Latiffah Zakaria; Hao Zhang; Ning Zhang; Sean Zhang; Xue Zhang. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex. Phytopathology® 2020, 1 .
AMA StyleDavid M Geiser, Abdullah Al-Hatmi, Takayuki Aoki, Tsutomu Arie, Virgilio Balmas, Irene Barnes, Gary C Bergstrom, M.K. K. Bhattacharyya, Cheryl L. Blomquist, Robert Bowden, Balázs Brankovics, Daren W. Brown, Lester William Burgess, Kathryn Bushley, Mark Busman, José F. Cano-Lira, Joseph D. Carrillo, Hao-Xun Chang, Chi-Yu Chen, Wanquan Chen, Martin I. Chilvers, Sofia Noemi Chulze, Jeffrey J. Coleman, Christina A. Cuomo, Z. Wilhelm De Beer, G. Sybren De Hoog, Johanna Del Castillo-Múnera, Emerson Del Ponte, Javier Diéguez-Uribeondo, Antonio Di Pietro, Vérnonique Edel-Hermann, Wade H Elmer, Lynn Epstein, Akif Eskalen, Maria Carmela Esposto, Kathryne L. Everts, Sylvia P. Fernández-Pavía, Gilvan Ferreira Da Silva, Nora A Foroud, Gerda Fourie, Rasmus J.N. Frandsen, Stanley Freeman, Michael Freitag, Omer Frenkel, Kevin K Fuller, Tatiana Gagkaeva, Donald Max Gardiner, Anthony E. Glenn, Scott Gold, Tom Gordon, Nancy F. Gregory, Marieka Gryzenhout, Josep Guarro, Beth Gugino, Santiago Gutiérrez, Kim Hammond-Kosack, Linda J. Harris, Mónika Homa, Cheng-Fang Hong, László Hornok, Jenn-Wen Huang, Macit Ilkit, Adriaana Jacobs, Karin Jacobs, Cong Jiang, Maria Del Mar Jimenez-Gasco, Seogchan Kang, Matthew T Kasson, Kemal Kazan, John Carlyle Kennell, Hyeseon Kim, Harold Corby Kistler, Gretchen A. Kuldau, Tomasz Kulik, Oliver Kurzai, Imane Laraba, Matthew H. Laurence, Theresa Yun Lee, Yin-Won Lee, Yong-Hwan Lee, John F. Leslie, Edward C.Y. Liew, Lily W. Lofton, Antonio Logrieco, Manuel Sánchez López-Berges, Alicia Graciela Luque, Erik Lysøe, Li-Jun Ma, Robert E Marra, Frank N. Martin, Sara Ruth May, Susan McCormick, Chyanna T McGee, Jacques F. Meis, Quirico Migheli, Nik Mohd Izham Mohamed Nor, Michel Monod, Antonio Moretti, Diane Mostert, Giuseppina. Mulé, Françoise Munaut, Gary P Munkvold, Paul Nicholson, Marcio Nucci, Kerry O'Donnell, Matias Pasquali, Ludwig H. Pfenning, Anna Prigitano, Robert Proctor, Stéphane Ranque, Stephen Rehner, Martijn Rep, Gerardo Rodríguez-Alvarado, Lindy J Rose, Mitchell George Roth, Carmen Ruiz-Roldán, Amgad A Saleh, Baharuddin Salleh, Hyunkyu Sang, Mercedes Scandiani, Jonathan Scauflaire, David Schmale, Dylan Pg Short, Adnan Šišić, Jason Smith, Christopher W. Smyth, Hokyoung Son, Ellie Spahr, Jason E Stajich, Emma Steenkamp, Christian Steinberg, Rajagopal Subramaniam, Haruhisa Suga, Brett Anthony Summerell, Antonella Susca, Cassandra Lynn Swett, Christopher Toomajian, Terry Jarianna Torres-Cruz, Anna Maria Tortorano, Martin Urban, Lisa J. Vaillancourt, Gary E Vallad, Theo Van Der Lee, Dan Vanderpool, Anne D. Van Diepeningen, Martha Vaughan, Eduard Venter, Marcele Vermeulen, Paul E. Verweij, Altus Viljoen, Cees Waalwijk, Emma C. Wallace, Grit Walther, Jie Wang, Todd Ward, Brian Wickes, Nathan P. Wiederhold, Michael J. Wingfield, Ana K.M. Wood, Jin-Rong Xu, X. B. Yang, Tapani Yli-Matilla, Sung-Hwan Yun, Latiffah Zakaria, Hao Zhang, Ning Zhang, Sean Zhang, Xue Zhang. Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex. Phytopathology®. 2020; ():1.
Chicago/Turabian StyleDavid M Geiser; Abdullah Al-Hatmi; Takayuki Aoki; Tsutomu Arie; Virgilio Balmas; Irene Barnes; Gary C Bergstrom; M.K. K. Bhattacharyya; Cheryl L. Blomquist; Robert Bowden; Balázs Brankovics; Daren W. Brown; Lester William Burgess; Kathryn Bushley; Mark Busman; José F. Cano-Lira; Joseph D. Carrillo; Hao-Xun Chang; Chi-Yu Chen; Wanquan Chen; Martin I. Chilvers; Sofia Noemi Chulze; Jeffrey J. Coleman; Christina A. Cuomo; Z. Wilhelm De Beer; G. Sybren De Hoog; Johanna Del Castillo-Múnera; Emerson Del Ponte; Javier Diéguez-Uribeondo; Antonio Di Pietro; Vérnonique Edel-Hermann; Wade H Elmer; Lynn Epstein; Akif Eskalen; Maria Carmela Esposto; Kathryne L. Everts; Sylvia P. Fernández-Pavía; Gilvan Ferreira Da Silva; Nora A Foroud; Gerda Fourie; Rasmus J.N. Frandsen; Stanley Freeman; Michael Freitag; Omer Frenkel; Kevin K Fuller; Tatiana Gagkaeva; Donald Max Gardiner; Anthony E. Glenn; Scott Gold; Tom Gordon; Nancy F. Gregory; Marieka Gryzenhout; Josep Guarro; Beth Gugino; Santiago Gutiérrez; Kim Hammond-Kosack; Linda J. Harris; Mónika Homa; Cheng-Fang Hong; László Hornok; Jenn-Wen Huang; Macit Ilkit; Adriaana Jacobs; Karin Jacobs; Cong Jiang; Maria Del Mar Jimenez-Gasco; Seogchan Kang; Matthew T Kasson; Kemal Kazan; John Carlyle Kennell; Hyeseon Kim; Harold Corby Kistler; Gretchen A. Kuldau; Tomasz Kulik; Oliver Kurzai; Imane Laraba; Matthew H. Laurence; Theresa Yun Lee; Yin-Won Lee; Yong-Hwan Lee; John F. Leslie; Edward C.Y. Liew; Lily W. Lofton; Antonio Logrieco; Manuel Sánchez López-Berges; Alicia Graciela Luque; Erik Lysøe; Li-Jun Ma; Robert E Marra; Frank N. Martin; Sara Ruth May; Susan McCormick; Chyanna T McGee; Jacques F. Meis; Quirico Migheli; Nik Mohd Izham Mohamed Nor; Michel Monod; Antonio Moretti; Diane Mostert; Giuseppina. Mulé; Françoise Munaut; Gary P Munkvold; Paul Nicholson; Marcio Nucci; Kerry O'Donnell; Matias Pasquali; Ludwig H. Pfenning; Anna Prigitano; Robert Proctor; Stéphane Ranque; Stephen Rehner; Martijn Rep; Gerardo Rodríguez-Alvarado; Lindy J Rose; Mitchell George Roth; Carmen Ruiz-Roldán; Amgad A Saleh; Baharuddin Salleh; Hyunkyu Sang; Mercedes Scandiani; Jonathan Scauflaire; David Schmale; Dylan Pg Short; Adnan Šišić; Jason Smith; Christopher W. Smyth; Hokyoung Son; Ellie Spahr; Jason E Stajich; Emma Steenkamp; Christian Steinberg; Rajagopal Subramaniam; Haruhisa Suga; Brett Anthony Summerell; Antonella Susca; Cassandra Lynn Swett; Christopher Toomajian; Terry Jarianna Torres-Cruz; Anna Maria Tortorano; Martin Urban; Lisa J. Vaillancourt; Gary E Vallad; Theo Van Der Lee; Dan Vanderpool; Anne D. Van Diepeningen; Martha Vaughan; Eduard Venter; Marcele Vermeulen; Paul E. Verweij; Altus Viljoen; Cees Waalwijk; Emma C. Wallace; Grit Walther; Jie Wang; Todd Ward; Brian Wickes; Nathan P. Wiederhold; Michael J. Wingfield; Ana K.M. Wood; Jin-Rong Xu; X. B. Yang; Tapani Yli-Matilla; Sung-Hwan Yun; Latiffah Zakaria; Hao Zhang; Ning Zhang; Sean Zhang; Xue Zhang. 2020. "Phylogenomic analysis of a 55.1 kb 19-gene dataset resolves a monophyletic Fusarium that includes the Fusarium solani Species Complex." Phytopathology® , no. : 1.
Laboratory of Mycology and Phytopathology, All-RussianInstitute of Plant Protection (VIZR), St. Petersburg, Pushkin, Russia Tatiana Yu Gagkaeva Molecular Plant Biology, Department of Biochemistry, Universityof Turku, Turku, Finland T. Yli-Mattila You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar Correspondence to Tatiana Yu Gagkaeva. The online version of the original article can be found at https://doi.org/10.1007/s10658-020-02118-2 Reprints and Permissions Gagkaeva, T.Y., Yli-Mattila, T. Correction to: Emergence of Fusarium verticillioides in Finland. Eur J Plant Pathol 158, 1059 (2020). https://doi.org/10.1007/s10658-020-02139-x Download citation Published: 27 October 2020 Issue Date: December 2020 DOI: https://doi.org/10.1007/s10658-020-02139-x
Tatiana Yu Gagkaeva; T. Yli-Mattila. Correction to: Emergence of Fusarium verticillioides in Finland. European Journal of Plant Pathology 2020, 158, 1059 -1059.
AMA StyleTatiana Yu Gagkaeva, T. Yli-Mattila. Correction to: Emergence of Fusarium verticillioides in Finland. European Journal of Plant Pathology. 2020; 158 (4):1059-1059.
Chicago/Turabian StyleTatiana Yu Gagkaeva; T. Yli-Mattila. 2020. "Correction to: Emergence of Fusarium verticillioides in Finland." European Journal of Plant Pathology 158, no. 4: 1059-1059.
In summer 2019, perithecia superficial on the leaf sheath near the base of the stem were visually observed on stalks of winter wheat cultivar Ceylon in Southwest Finland. Mycological examination and translation elongation factor 1-alpha gene sequence of single ascospore isolates determined that they were Fusarium verticillioides (Sacc.) Nirenberg (teleomorph: Gibberella moniliformis Wineland). According to the published information, neither the anamorph nor the teleomorph stages of this pathogen have been previously detected in cereals in Finland and Scandinavian countries. The emergence of this fungus is probably caused by the influence of climate change, which may favour the distribution, development and adaptation of introduced mycotoxigenic fungi. Detection of a fumonisin-producing F. verticillioides allows the prediction of changes in the pattern of grain mycotoxin contamination in northern European countries. A mutation in sexual reproduction was also detected. The mature perithecia contained three types of spores: straight one- or three-septate and abnormal rounded ascospores.
Tatiana Yu. Gagkaeva; T. Yli-Mattila. Emergence of Fusarium verticillioides in Finland. European Journal of Plant Pathology 2020, 158, 1 -7.
AMA StyleTatiana Yu. Gagkaeva, T. Yli-Mattila. Emergence of Fusarium verticillioides in Finland. European Journal of Plant Pathology. 2020; 158 (4):1-7.
Chicago/Turabian StyleTatiana Yu. Gagkaeva; T. Yli-Mattila. 2020. "Emergence of Fusarium verticillioides in Finland." European Journal of Plant Pathology 158, no. 4: 1-7.
Background. The requirement for high-quality oat grain for food production leads to the need for studying the resistance of this cereal crop to harmful diseases and for the development of new productive cultivars.Materials and methods. A comparative characterization was performed among the breeding lines of naked oats from Nemchinovka Federal Research Center according to the parameters of microbiological purity of grain. The mycological method was applied for the analysis of infection in the oat grain, and fungal species composition was identified. The DNA amount of toxin-producing species from genus Fusarium Link in the grain was detected using the real-time PCR, and the amount of mycotoxins was measured by the enzyme immunoassay.Results. The fungi of Alternaria Nees, Fusarium, Cladosporium Link, Cochliobolus Drechsler, Epicoccum Link were associated with oat grain. Uniquely high infection of the grain by F. langsethiae Torp & Nirenberg (14%) was found under the natural conditions of the Central Region of Russia. The differences revealed among the analyzed breeding lines and cultivars in the DNA amount of F. langsethiae and F. sporotrichioides Sherb. were from 1.3×10-4 to 7.2×10-3 pg/ng in the total DNA, and from 5 to 1230 μg/kg in the content of T-2/ HT-2 toxins.Conclusion. All naked oat breeding lines were relatively resistant to Fusarium infection and proved the best as regards the mycotoxin contamination of the grain against the reference hulled cultivar ‘Yakov’. In the competitive variety trials, three lines – 66h2618, 54h2476 and 70h2613 – were characterized as relatively resistant to Fusarium disease, compared with the naked oat reference ‘Vyatsky golozerny’.
O. P. Gavrilova; T. Yu. Gagkaeva; A. S. Orina; A. S. Markova; A. D. Kabashov; I. G. Loskutov. Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province. Proceedings on applied botany, genetics and breeding 2020, 181, 134-144 .
AMA StyleO. P. Gavrilova, T. Yu. Gagkaeva, A. S. Orina, A. S. Markova, A. D. Kabashov, I. G. Loskutov. Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province. Proceedings on applied botany, genetics and breeding. 2020; 181 (2):134-144.
Chicago/Turabian StyleO. P. Gavrilova; T. Yu. Gagkaeva; A. S. Orina; A. S. Markova; A. D. Kabashov; I. G. Loskutov. 2020. "Mycobiota in Mycobiota in the grain of the oat breeding lines produced in 2019 in competitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Provincepetitive variety trials on the fields of Nemchinovka Federal Research Center, Moscow Province." Proceedings on applied botany, genetics and breeding 181, no. 2: 134-144.
This study aimed to assess the contamination of Fabaceae plants with fungi and mycotoxins, which have a negative effect on the health of consumers of plant products. Quantitative PCR was used to analyse the DNA of Alternaria, Cladosporium and Fusarium fungi in 69 plant samples harvested from four Russian regions in 2015. Additionally, mycotoxins were analysed using ELISA. Cladosporium fungi DNA was found in all the analysed samples; the occurrence of Alternaria and Fusarium DNA was 84% and 51%, respectively. Mycotoxin alternariol was detected in 100% and emodin in 90% of the samples. The occurrence of deoxynivalenol, diacetoxyscirpenol and T-2/HT-2 mycotoxins was 32%, 42% and 41%, respectively. Plant species had no significant effect on the Alternaria, Cladosporium and Fusarium DNA content of the samples; however, the alternariol, emodin, deoxynivalenol and diacetoxyscirpenol contents differed significantly between plant species. The geographical origin had a statistically significant effect on the Alternaria and Fusarium DNA contents, likely due to differences in weather conditions.
Aleksandra Orina; Olga P. Gavrilova; Tatiana Gagkaeva; Aleksey Burkin; Galina Kononenko. The contamination of Fabaceae plants with fungi and mycotoxins. Agricultural and Food Science 2020, 29, 265–275 -265–275.
AMA StyleAleksandra Orina, Olga P. Gavrilova, Tatiana Gagkaeva, Aleksey Burkin, Galina Kononenko. The contamination of Fabaceae plants with fungi and mycotoxins. Agricultural and Food Science. 2020; 29 (3):265–275-265–275.
Chicago/Turabian StyleAleksandra Orina; Olga P. Gavrilova; Tatiana Gagkaeva; Aleksey Burkin; Galina Kononenko. 2020. "The contamination of Fabaceae plants with fungi and mycotoxins." Agricultural and Food Science 29, no. 3: 265–275-265–275.
The biological characterization of Microdochium majus, M. nivale, and M. seminicola strains with wide geographical origins showed the diversity of their pathogenic properties and metabolite compounds, allowing them to exist in their habitats. Significant differences in the ability of Microdochium fungi to cause lesions on wheat and oat leaves were found. The intensity of symptoms depended on the species and substrate origin of the strains. On average M. seminicola strains were able to cause less leaf necrosis than M. majus and M nivale. The volatile organic compound (VOC) profile of Microdochium fungi included 29 putative fungal metabolites. The spectrum of the identified VOCs in M. seminicola strains was much richer than that in M. majus and M. nivale strains. In addition, the strains of M. seminicola emitted at least six sesquiterpenes. Mycotoxin analysis by HPLC‐MS/MS revealed that the analysed Microdochium strains did not produce any toxic metabolites typically produced by filamentous fungi.
Olga P. Gavrilova; Aleksandra S. Orina; Elizaveta D. Kessenikh; Lyudmila K. Gustyleva; Elena I. Savelieva; Nadezhda N. Gogina; Tatiana Yu. Gagkaeva. Diversity of Physiological and Biochemical Characters of Microdochium Fungi. Chemistry & Biodiversity 2020, 17, 1 .
AMA StyleOlga P. Gavrilova, Aleksandra S. Orina, Elizaveta D. Kessenikh, Lyudmila K. Gustyleva, Elena I. Savelieva, Nadezhda N. Gogina, Tatiana Yu. Gagkaeva. Diversity of Physiological and Biochemical Characters of Microdochium Fungi. Chemistry & Biodiversity. 2020; 17 (8):1.
Chicago/Turabian StyleOlga P. Gavrilova; Aleksandra S. Orina; Elizaveta D. Kessenikh; Lyudmila K. Gustyleva; Elena I. Savelieva; Nadezhda N. Gogina; Tatiana Yu. Gagkaeva. 2020. "Diversity of Physiological and Biochemical Characters of Microdochium Fungi." Chemistry & Biodiversity 17, no. 8: 1.
In total, 46 Microdochium strains from five different geographic regions of Russia were explored with respect to genetic diversity, morphology, and secondary metabolites. Based on the results of PCR, 59% and 28% of the strains were identified as M. nivale and M. majus, respectively. As a result of sequencing four genome regions, namely ITS, LSU, BTUB, and RPB2 (2778 bp), five genetically and phenotypically similar strains from Western Siberia were identified as M. seminicola, which, according to our findings, is the prevalent Microdochium species in this territory. This is the first record of M. seminicola in Russia. Attempts were made to distinguish between Microdochium species and to identify species-specific morphological characteristics in the anamorph and teleomorph stages and physiological properties. We examined the occurrence frequency of conidia with different numbers of septa in the strains of Microdochium. The predominance of three-septate macroconidia in M. majus was higher than that in M. nivale and typically exceeded 60% occurrence. Most M. majus and M. nivale strains formed walled protoperithecia on wheat stems. Only three strains of M. majus and one strain each of M. nivale and M. seminicola produced mature perithecia. The growth rate of M. seminicola strains was significantly lower on agar media at 5–25 °C than those of M. majus and M. nivale strains. Multimycotoxin analysis by HPLC-MS/MS revealed that the strains of three Microdochium species did not produce any toxic metabolites.
Tatiana Yu. Gagkaeva; Aleksandra S. Orina; Olga P. Gavrilova; Nadezhda N. Gogina. Evidence of Microdochium Fungi Associated with Cereal Grains in Russia. Microorganisms 2020, 8, 340 .
AMA StyleTatiana Yu. Gagkaeva, Aleksandra S. Orina, Olga P. Gavrilova, Nadezhda N. Gogina. Evidence of Microdochium Fungi Associated with Cereal Grains in Russia. Microorganisms. 2020; 8 (3):340.
Chicago/Turabian StyleTatiana Yu. Gagkaeva; Aleksandra S. Orina; Olga P. Gavrilova; Nadezhda N. Gogina. 2020. "Evidence of Microdochium Fungi Associated with Cereal Grains in Russia." Microorganisms 8, no. 3: 340.
Trichothecenes are sesquiterpenoid mycotoxins produced by fungi from the order Hypocreales, including members of the Fusarium genus that infect cereal grain crops. Different trichothecene-producing Fusarium species and strains have different trichothecene chemotypes belonging to the Type A and B class. These fungi cause a disease of small grain cereals, called Fusarium head blight, and their toxins contaminate host tissues. As potent inhibitors of eukaryotic protein synthesis, trichothecenes pose a health risk to human and animal consumers of infected cereal grains. In 2009, Foroud and Eudes published a review of trichothecenes in cereal grains for human consumption. As an update to this review, the work herein provides a comprehensive and multi-disciplinary review of the Fusarium trichothecenes covering topics in chemistry and biochemistry, pathogen biology, trichothecene toxicity, molecular mechanisms of resistance or detoxification, genetics of resistance and breeding strategies to reduce their contamination of wheat and barley.
Nora A. Foroud; Danica Baines; Tatiana Y. Gagkaeva; Nehal Thakor; Ana Badea; Barbara Steiner; Maria Bürstmayr; Hermann Bürstmayr. Trichothecenes in Cereal Grains – An Update. Toxins 2019, 11, 634 .
AMA StyleNora A. Foroud, Danica Baines, Tatiana Y. Gagkaeva, Nehal Thakor, Ana Badea, Barbara Steiner, Maria Bürstmayr, Hermann Bürstmayr. Trichothecenes in Cereal Grains – An Update. Toxins. 2019; 11 (11):634.
Chicago/Turabian StyleNora A. Foroud; Danica Baines; Tatiana Y. Gagkaeva; Nehal Thakor; Ana Badea; Barbara Steiner; Maria Bürstmayr; Hermann Bürstmayr. 2019. "Trichothecenes in Cereal Grains – An Update." Toxins 11, no. 11: 634.
Wheat grains collected in three regions of Russia—Volga, Ural, and West Siberia—were analyzed for triangulation of methods in analysis of toxigenic Fusarium species. The presence of fungi and quantitative content of their biomass were detected by using various analytical methods, including a mycological and immunochemical methods, and quantitative PCR. Additionally, an enzyme-linked immunosorbent assay and high-performance liquid chromatography with tandem mass spectrometry were applied for determination of mycotoxins. Regional differences were found regarding the contamination of wheat grain by Fusarium fungi and their toxins. The most important observation was the detection of F. graminearum in the Ural and West Siberian regions, where this pathogen had not been found previously. A maximum damaged grains by F. graminearum and F. sporotrichioides was found in the grain samples from West Siberia. The DNA of F. graminearum was detected in 19.2% and DNA of F. sporotrichioides was found in 84.1% of the analyzed grain samples. The amount of Fusarium antigens in the grain samples from the West Siberian region was 7–8 times higher than in the grain samples from the other two regions. Significant contamination of the grain with deoxynivalenol and T-2/HT-2 toxins (maximum contents were 2239 ppb and 199 ppb, respectively) was detected in the West Siberian region.
Tatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins 2019, 11, 252 .
AMA StyleTatiana Gagkaeva, Olga Gavrilova, Aleksandra Orina, Yuri Lebedin, Ilya Shanin, Pavel Petukhov, Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins. 2019; 11 (5):252.
Chicago/Turabian StyleTatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. 2019. "Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia." Toxins 11, no. 5: 252.
T. Y. Gagkaeva; Olga P. Gavrilova; A. S. Orina. First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia. Plant Disease 2019, 103, 588 -588.
AMA StyleT. Y. Gagkaeva, Olga P. Gavrilova, A. S. Orina. First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia. Plant Disease. 2019; 103 (3):588-588.
Chicago/Turabian StyleT. Y. Gagkaeva; Olga P. Gavrilova; A. S. Orina. 2019. "First Report of Fusarium globosum Associated with Barley Grain in the Southwestern Part of Siberia." Plant Disease 103, no. 3: 588-588.
Fusarium avenaceum and closely related species are common fungi on various plants, cultivated in different climatic regions. The aim of this study was to determine the taxonomic affiliations of the F. avenaceum, Fusarium arthrosporioides, and Fusarium anguioides strains by using morphological, physiological and molecular-genetic approaches. Twenty-six single-spored morphologically identified strains, which were mainly from cereals, were investigated in order to find out, if they belong to a separate species. Pathogenicity of strains to wheat seedlings and ISSR (Inter Simple Sequence Repeats) fingerprint and beta-tubulin DNA sequence patterns were analyzed. According to phylogenetic analyses, the strains could be divided into two big groups consisting of mostly F. avenaceum or F. anguioides strains. F. arthrosporioides was not detected as a separate species by the sum of the characters. F. anguioides was characterized as a separate species, which could be identified by morphological and molecular data. High genetic diversity of the F. avenaceum species complex was revealed. One F. anguioides strain (rudbeckia, Vladivostok, Russia), had an identical beta-tubulin sequence with two previously sequenced strains of Fusarium tricinctum species complex, which were isolated from dicotyledonous plants in Asia.
Tapani Yli-Mattila; Taha Hussien; Olga Gavrilova; Tatiana Gagkaeva. Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains. Pathogens 2018, 7, 94 .
AMA StyleTapani Yli-Mattila, Taha Hussien, Olga Gavrilova, Tatiana Gagkaeva. Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains. Pathogens. 2018; 7 (4):94.
Chicago/Turabian StyleTapani Yli-Mattila; Taha Hussien; Olga Gavrilova; Tatiana Gagkaeva. 2018. "Morphological and Molecular Variation Between Fusarium avenaceum, Fusarium arthrosporioides and Fusarium anguioides Strains." Pathogens 7, no. 4: 94.
In this study, naturally and artificially inoculated winter wheat varieties were studied with respect to their productivity and resistance to Fusarium head blight (FHB). We used the following set of disease assessment parameters: the percentage of visually and latent Fusarium-damaged grains (FDG); the DNA content of Fusarium fungi; the productivity of inoculated plants compared with non-inoculated plants; and the amount of mycotoxins in the grain. In case of naturally infected grains, the average FDG was found to be about 6.1 % (range of 0–15 %). The amount of DNA of Fusarium graminearum was found to be in the range of (1.1–42.7) × 10–5 ng/ng wheat DNA. The mycotoxin deoxynivalenol (DON) was detected in 15 samples of grain from plants that were grown under natural infection. The maximum DON amount was found to be 420 μg/kg. Fumonisin B1 (FB1) was not be detected in naturally infected grain. In case of artificially inoculated plants, the average FDG was found to be 25.8 % (2–54 %). The amount of F. graminearum DNA was found to be significantly higher (4.24– 49.8) × 10–3 ng/ng than it was detected in grain of non-inoculated plants. The wheat varieties inoculated with F. graminearum contained DON in high amounts from 20255 to 79245 μg/kg. Furthermore, a significant amount of FB1 was detected in all wheat varieties in the range of 980–20326 μg/kg. Among the analysed wheat varieties, Adel was characterized to be the most resistant to fungal infection as well as to the contamination by mycotoxins. Antonina, Lebed and Pamyat varieties were classified more relatively resistant than that of other varieties, and Utrish variety was found to be the most susceptible to FHB. The similar resistance of wheat varieties against F. graminearum and F. verticillioides infection was recorded, and the interactions between the fungi during the colonization of grain were shown.
T. Yu. Gagkaeva; A. S. Orina; O. P. Gavrilova; I. B. Ablova; L. A. Bespalova. Characterization of resistance of winter wheat varieties to Fusarium head blight. Vavilov Journal of Genetics and Breeding 2018, 22, 685 -692.
AMA StyleT. Yu. Gagkaeva, A. S. Orina, O. P. Gavrilova, I. B. Ablova, L. A. Bespalova. Characterization of resistance of winter wheat varieties to Fusarium head blight. Vavilov Journal of Genetics and Breeding. 2018; 22 (6):685-692.
Chicago/Turabian StyleT. Yu. Gagkaeva; A. S. Orina; O. P. Gavrilova; I. B. Ablova; L. A. Bespalova. 2018. "Characterization of resistance of winter wheat varieties to Fusarium head blight." Vavilov Journal of Genetics and Breeding 22, no. 6: 685-692.
A.A. Burkin; G.P. Kononenko; O.P. Gavrilova; T.Yu. Gagkaeva. MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA. Sel'skokhozyaistvennaya Biologiya 2017, 52, 409 -417.
AMA StyleA.A. Burkin, G.P. Kononenko, O.P. Gavrilova, T.Yu. Gagkaeva. MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA. Sel'skokhozyaistvennaya Biologiya. 2017; 52 (2):409-417.
Chicago/Turabian StyleA.A. Burkin; G.P. Kononenko; O.P. Gavrilova; T.Yu. Gagkaeva. 2017. "MYCOTOXINS IN THE LEGUMES OF NATURAL FODDER OF THE EUROPEAN RUSSIA." Sel'skokhozyaistvennaya Biologiya 52, no. 2: 409-417.
Буркин Буркин; Г.п. Кононенко; О.п. Гаврилова; Т.ю. Гагкаева. МИКОТОКСИНЫ В БОБОВЫХ ТРАВАХ ЕСТЕСТВЕННЫХ КОРМОВЫХ УГОДИЙ ЕВРОПЕЙСКОЙ РОССИИ. Sel'skokhozyaistvennaya Biologiya 2017, 52, 409 -417.
AMA StyleБуркин Буркин, Г.п. Кононенко, О.п. Гаврилова, Т.ю. Гагкаева. МИКОТОКСИНЫ В БОБОВЫХ ТРАВАХ ЕСТЕСТВЕННЫХ КОРМОВЫХ УГОДИЙ ЕВРОПЕЙСКОЙ РОССИИ. Sel'skokhozyaistvennaya Biologiya. 2017; 52 (2):409-417.
Chicago/Turabian StyleБуркин Буркин; Г.п. Кононенко; О.п. Гаврилова; Т.ю. Гагкаева. 2017. "МИКОТОКСИНЫ В БОБОВЫХ ТРАВАХ ЕСТЕСТВЕННЫХ КОРМОВЫХ УГОДИЙ ЕВРОПЕЙСКОЙ РОССИИ." Sel'skokhozyaistvennaya Biologiya 52, no. 2: 409-417.
Analysis of 49 strains of Fusarium langsethiae originating from northern Europe (Russia, Finland, Sweden, UK, Norway, and Latvia) revealed the presence of spontaneous auxotrophic mutants that reflect natural intraspecific diversity. Our investigations detected that 49.0% of F. langsethiae strains were auxotrophic mutants for biotin, and 8.2% of the strains required thiamine as a growth factor. They failed to grow on vitamin-free media. For both prototrophic and auxotrophic strains, no growth defect was observed in rich organic media. Without essential vitamins, a significant reduction in the growth of the auxotrophic strains results in a decrease of the formation of T-2 toxin and diacetoxyscirpenol. In addition, all analysed F. langsethiae strains were distinguished into two subgroups based on PCR product sizes. According to our results, 26 and 23 strains of F. langsethiae belong to subgroups I and II respectively. We determined that the deletion in the intergenic spacer (IGS) region of the rDNA of F. langsethiae belonging to subgroup II is linked with temperature sensitivity and causes a decrease in strain growth at 30 °C. Four thiamine auxotrophic strains were found in subgroup I, while 21 biotin auxotrophic strains were detected in subgroups II. To the best of our knowledge, the spontaneous mutations in F. langsethiae observed in the present work have not been previously reported.
Olga Gavrilova; Anna Skritnika; Tatiana Gagkaeva. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms 2017, 5, 14 .
AMA StyleOlga Gavrilova, Anna Skritnika, Tatiana Gagkaeva. Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae. Microorganisms. 2017; 5 (2):14.
Chicago/Turabian StyleOlga Gavrilova; Anna Skritnika; Tatiana Gagkaeva. 2017. "Identification and Characterization of Spontaneous Auxotrophic Mutants in Fusarium langsethiae." Microorganisms 5, no. 2: 14.
The compositions of volatile organic compounds (VOCs) emitted by Fusarium fungi (F. langsethiae, F. sibiricum, F. poae, and F. sporotrichioides) grown on two nutritive substrates: potato sucrose agar (PSA) and autoclaved wheat kernels (WK) were investigated. The culturing of fungi and study of their VOC emissions were performed in chromatographic vials at room temperature (23 – 24 °C) and the VOCs were sampled by solid-phase microextraction on a 85 μm Carboxen/Polydimethylsiloxane fiber. GC/MS was performed using a 60-m HP-5 capillary column. Components of the VOC mixture were identified by electron impact mass spectra and chromatographic retention indices (RIs).The most abundant components of the VOC mixture emitted by Fusarium fungi are ethanol, ethyl acetate, isobutanol, 3-methylbutan-1-ol, 2-methylbutan-1-ol, ethyl 3-methylbutanoate, terpenes with M 136, sesquiterpenes with M 204 (a total of about 25), and trichodiene. It was found that the strains grown on PSA emit a wider spectrum and larger amount of VOCs compared with those grown on wheat kernels. F. langsethiae strain is the most active VOC producer on both substrates. The use of SPME and GC/MS also offers the potential for differentiation of fungal species and strains.This article is protected by copyright. All rights reserved.
Elena I. Savelieva; Liudmila K. Gustyleva; Elizaveta Kessenikh; Natalya S. Khlebnikova; John Leffingwell; Olga P. Gavrilova; Tatiana Yu. Gagkaeva; Ludmila K. Gustyleva. Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates. Chemistry & Biodiversity 2016, 13, 891 -903.
AMA StyleElena I. Savelieva, Liudmila K. Gustyleva, Elizaveta Kessenikh, Natalya S. Khlebnikova, John Leffingwell, Olga P. Gavrilova, Tatiana Yu. Gagkaeva, Ludmila K. Gustyleva. Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates. Chemistry & Biodiversity. 2016; 13 (7):891-903.
Chicago/Turabian StyleElena I. Savelieva; Liudmila K. Gustyleva; Elizaveta Kessenikh; Natalya S. Khlebnikova; John Leffingwell; Olga P. Gavrilova; Tatiana Yu. Gagkaeva; Ludmila K. Gustyleva. 2016. "Study of the Vapor Phase OverFusariumFungi Cultured on Various Substrates." Chemistry & Biodiversity 13, no. 7: 891-903.
O.P. Gavrilova; Ph.B. Gannibal; T.Yu. Gagkaeva. Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY. Sel'skokhozyaistvennaya Biologiya 2016, 51, 111 -118.
AMA StyleO.P. Gavrilova, Ph.B. Gannibal, T.Yu. Gagkaeva. Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY. Sel'skokhozyaistvennaya Biologiya. 2016; 51 (1):111-118.
Chicago/Turabian StyleO.P. Gavrilova; Ph.B. Gannibal; T.Yu. Gagkaeva. 2016. "Fusarium AND Alternaria FUNGI IN GRAIN OF OATS GROWN IN THE NORTH-WESTERN RUSSIA REGARDING CULTIVAR SPECIFICITY." Sel'skokhozyaistvennaya Biologiya 51, no. 1: 111-118.
О.п. Гаврилова; Ф.б. Ганнибал; Т.ю. Гагкаева. ЗАРАЖЕННОСТЬ ЗЕРНА ОВСА ГРИБАМИ Fusarium И Alternaria И ЕЕ СОРТОВАЯ СПЕЦИФИКА В УСЛОВИЯХ СЕВЕРО-ЗАПАДА РОССИИ. Sel'skokhozyaistvennaya Biologiya 2016, 51, 111 -118.
AMA StyleО.п. Гаврилова, Ф.б. Ганнибал, Т.ю. Гагкаева. ЗАРАЖЕННОСТЬ ЗЕРНА ОВСА ГРИБАМИ Fusarium И Alternaria И ЕЕ СОРТОВАЯ СПЕЦИФИКА В УСЛОВИЯХ СЕВЕРО-ЗАПАДА РОССИИ. Sel'skokhozyaistvennaya Biologiya. 2016; 51 (1):111-118.
Chicago/Turabian StyleО.п. Гаврилова; Ф.б. Ганнибал; Т.ю. Гагкаева. 2016. "ЗАРАЖЕННОСТЬ ЗЕРНА ОВСА ГРИБАМИ Fusarium И Alternaria И ЕЕ СОРТОВАЯ СПЕЦИФИКА В УСЛОВИЯХ СЕВЕРО-ЗАПАДА РОССИИ." Sel'skokhozyaistvennaya Biologiya 51, no. 1: 111-118.
The quality of grass samples used for animal feed by combining mycotoxin measures and mycological determination of mycobiota were explored. The samples of the plant material were collected in 2014 in two stages: before the first mowing (May–June) and before the second one (July–August) from the fields of stock-farms located in northwestern part of the Russia. All samples were divided into three types: grasses, mixture of different grasses and clover, alfalfa mixed with timothy. The occurrence of aflatoxin B1, alternariol, citrinin, cyclopiazonic acid, deoxynivalenol, diacetoxyscirpenol, emodin, ergot alkaloids, fumonisins, mycophenolic acid, ochratoxin A, PR-toxin, roridin A, sterigmatocystin, T-2 toxin, and zearalenone were determined using ELISA. The multiple fungal and mycotoxin contaminations are already formed in plant tissues by the moment of first mowing. The complexes of mycotoxins including up to 14–16 components and the combined character of plant contamination quite correspond to the taxonomic variety of mycobiota.
Galina Kononenko; Alexey Burkin; Olga Gavrilova; Tatiana Gagkaeva. Fungal species and multiple mycotoxin contamination of cultivated forage crops. Agricultural and Food Science 2015, 24, 323–330 -323–330.
AMA StyleGalina Kononenko, Alexey Burkin, Olga Gavrilova, Tatiana Gagkaeva. Fungal species and multiple mycotoxin contamination of cultivated forage crops. Agricultural and Food Science. 2015; 24 (4):323–330-323–330.
Chicago/Turabian StyleGalina Kononenko; Alexey Burkin; Olga Gavrilova; Tatiana Gagkaeva. 2015. "Fungal species and multiple mycotoxin contamination of cultivated forage crops." Agricultural and Food Science 24, no. 4: 323–330-323–330.