Maize is a globally important cereal and fodder crop. Accurate monitoring of maize planting densities is vital for informed decision-making by agricultural managers. Compared to traditional manual methods for collecting crop trait parameters, approaches using unmanned aerial vehicle (UAV) remote sensing can enhance the efficiency, minimize personnel costs and biases, and, more importantly, rapidly provide density maps of maize fields. This study involved the following steps: (1) Two UAV remote sensing-based methods were developed for monitoring maize planting densities. These methods are based on (a) ultrahigh-definition imagery combined with object detection (UHDI-OD) and (b) multispectral remote sensing combined with machine learning (Multi-ML) for the monitoring of maize planting densities. (2) The maize planting density measurements, UAV ultrahigh-definition imagery, and multispectral imagery collection were implemented at a maize breeding trial site. Experimental testing and validation were conducted using the proposed maize planting density monitoring methods. (3) An in-depth analysis of the applicability and limitations of both methods was conducted to explore the advantages and disadvantages of the two estimation models. The study revealed the following findings: (1) UHDI-OD can provide highly accurate estimation results for maize densities (R² = 0.99, RMSE = 0.09 plants/m²). (2) Multi-ML provides accurate maize density estimation results by combining remote sensing vegetation indices (VIs) and gray-level co-occurrence matrix (GLCM) texture features (R² = 0.76, RMSE = 0.67 plants/m²). (3) UHDI-OD exhibits a high sensitivity to image resolution, making it unsuitable for use with UAV remote sensing images with pixel sizes greater than 2 cm. In contrast, Multi-ML is insensitive to image resolution and the model accuracy gradually decreases as the resolution decreases.

Maize, an important cereal crop and crucial industrial material, is widely used in various fields, including food, feed, and industry. Maize is also a highly adaptable crop, capable of thriving under various climatic and soil conditions. Against the backdrop of intensified climate change, studying the classification of maize growth stages can aid in adjusting planting strategies to enhance yield and quality. Accurate classification of the growth stages of maize breeding materials is important for enhancing yield and quality in breeding endeavors. Traditional remote sensing-based crop growth stage classifications mainly rely on time series vegetation index (VI) analyses; however, VIs are prone to saturation under high-coverage conditions. Maize phenotypic traits at different growth stages may improve the accuracy of crop growth stage classifications. Therefore, we developed a method for classifying maize growth stages during the vegetative growth phase by combining maize phenotypic traits with different classification algorithms. First, we tested various VIs, texture features (TFs), and combinations of VI and TF as input features to estimate the leaf chlorophyll content (LCC), leaf area index (LAI), and fractional vegetation cover (FVC). We determined the optimal feature inputs and estimation methods and completed crop height (CH) extraction. Then, we tested different combinations of maize phenotypic traits as input variables to determine their accuracy in classifying growth stages and to identify the optimal combination and classification method. Finally, we compared the proposed method with traditional growth stage classification methods based on remote sensing VIs and machine learning models. The results indicate that (1) when the VI+TFs are used as input features, random forest regression (RFR) shows a good estimation performance for the LCC (R²: 0.920, RMSE: 3.655 SPAD units, MAE: 2.698 SPAD units), Gaussian process regression (GPR) performs well for the LAI (R²: 0.621, RMSE: 0.494, MAE: 0.397), and linear regression (LR) exhibits a good estimation performance for the FVC (R²: 0.777, RMSE: 0.051, MAE: 0.040); (2) when using the maize LCC, LAI, FVC, and CH phenotypic traits to classify maize growth stages, the random forest (RF) classification method achieved the highest accuracy (accuracy: 0.951, precision: 0.951, recall: 0.951, F1: 0.951); and (3) the effectiveness of the growth stage classification based on maize phenotypic traits outperforms that of traditional remote sensing-based crop growth stage classifications.

Soybeans are rich in high-quality protein and raw materials for producing hundreds of chemical products. Consequently, soybean cultivation has gained widespread prevalence across diverse geographic regions. Soybean breeding necessitates the development of early-, standard-, and late-maturing cultivars to accommodate cultivation at various latitudes, thereby optimizing the utilization of solar radiation. In the practical process of determining the maturity of soybean breeding materials within the breeding field, the ripeness is assessed based on three critical criteria: pod moisture content, leaf color, and the degree of leaf shedding. These parameters reflect the crown structure, physicochemical parameters, and reproductive organ changes in soybeans during the maturation process. Therefore, methods for analyzing soybean maturity at the breeding plot scale should match the standards of agricultural experts to the maximum possible extent. This study presents a hyperspectral remote sensing approach for monitoring soybean maturity. We collected five periods of unmanned aerial vehicle (UAV)-based soybean canopy hyperspectral digital orthophoto maps (DOMs) and ground-level measurements of leaf chlorophyll content (LCC), flavonoids (Flav), and the nitrogen balance index (NBI) from a breeding farm. This study explores the following aspects: (1) the correlations between soybean LCC, NBI, Flav, and maturity; (2) the estimation of soybean LCC, NBI, and Flav using Gaussian process regression (GPR), partial least squares regression (PLSR), and random forest (RF) regression techniques; and (3) the application of threshold-based methods in conjunction with normalized difference vegetation index (NDVI)+LCC and NDVI+NBI for soybean maturity monitoring. The results of this study indicate the following: (1) Soybean LCC, NBI, and Flav are associated with maturity. LCC increases during the beginning bloom period (P1) to the beginning seed period (P3) and sharply decreases during the beginning maturity period (P4) stage. Flav continues to increase from P1 to P4. NBI remains relatively consistent from P1 to P3 and then drops rapidly during the P4 stage. (2) The GPR, PLSR, and RF methodologies yield comparable accuracy in estimating soybean LCC (coefficient of determination (R²): 0.737–0.832, root mean square error (RMSE): 3.35–4.202 Dualex readings), Flav (R²: 0.321–0.461, RMSE: 0.13–0.145 Dualex readings), and NBI (R²: 0.758–0.797, RMSE: 2.922–3.229 Dualex readings). (3) The combination of the threshold method with NDVI < 0.55 and NBI < 8.2 achieves the highest classification accuracy (accuracy = 0.934). Further experiments should explore the relationships between crop NDVI, the Chlorophyll Index, LCC, Flav, and NBI and crop maturity for different crops and ecological areas.

Timely and accurate monitoring of fractional vegetation cover (FVC), leaf chlorophyll content (LCC), and maturity of breeding material are essential for breeding companies. This study aimed to estimate LCC and FVC on the basis of remote sensing and to monitor maturity on the basis of LCC and FVC distribution. We collected UAV-RGB images at key growth stages of soybean, namely, the podding (P1), early bulge (P2), peak bulge (P3), and maturity (P4) stages. Firstly, based on the above multi-period data, four regression techniques, namely, partial least squares regression (PLSR), multiple stepwise regression (MSR), random forest regression (RF), and Gaussian process regression (GPR), were used to estimate the LCC and FVC, respectively, and plot the images in combination with vegetation index (VI). Secondly, the LCC images of P3 (non-maturity) were used to detect LCC and FVC anomalies in soybean materials. The method was used to obtain the threshold values for soybean maturity monitoring. Additionally, the mature and immature regions of soybean were monitored at P4 (mature stage) by using the thresholds of P3-LCC. The LCC and FVC anomaly detection method for soybean material presents the image pixels as a histogram and gradually removes the anomalous values from the tails until the distribution approaches a normal distribution. Finally, the P4 mature region (obtained from the previous step) is extracted, and soybean harvest monitoring is carried out in this region using the LCC and FVC anomaly detection method for soybean material based on the P4-FVC image. Among the four regression models, GPR performed best at estimating LCC (R²: 0.84, RMSE: 3.99) and FVC (R²: 0.96, RMSE: 0.08). This process provides a reference for the FVC and LCC estimation of soybean at multiple growth stages; the P3-LCC images in combination with the LCC and FVC anomaly detection methods for soybean material were able to effectively monitor soybean maturation regions (overall accuracy of 0.988, mature accuracy of 0.951, immature accuracy of 0.987). In addition, the LCC thresholds obtained by P3 were also applied to P4 for soybean maturity monitoring (overall accuracy of 0.984, mature accuracy of 0.995, immature accuracy of 0.955); the LCC and FVC anomaly detection method for soybean material enabled accurate monitoring of soybean harvesting areas (overall accuracy of 0.981, mature accuracy of 0.987, harvested accuracy of 0.972). This study provides a new approach and technique for monitoring soybean maturity in breeding fields.

Crop leaf chlorophyll content (LCC) and fractional vegetation cover (FVC) are crucial indicators for assessing crop health, growth development, and maturity. In contrast to the traditional manual collection of crop trait parameters, unmanned aerial vehicle (UAV) technology rapidly generates LCC and FVC maps for breeding materials, facilitating prompt assessments of maturity information. This study addresses the following research questions: (1) Can image features based on pretrained deep learning networks and ensemble learning enhance the estimation of remote sensing LCC and FVC? (2) Can the proposed adaptive normal maturity detection (ANMD) algorithm effectively monitor maize maturity based on LCC and FVC maps? We conducted the following tasks: (1) Seven phases (tassel initiation to maturity) of maize canopy orthoimages and corresponding ground-truth data for LCC and six phases of FVC using UAVs were collected. (2) Three features, namely vegetation indices (VI), texture features (TF) based on Gray Level Co-occurrence Matrix, and deep features (DF), were evaluated for LCC and FVC estimation. Moreover, the potential of four single-machine learning models and three ensemble models for LCC and FVC estimation was evaluated. (3) The estimated LCC and FVC were combined with the proposed ANMD to monitor maize maturity. The research findings indicate that (1) image features extracted from pretrained deep learning networks more accurately describe crop canopy structure information, effectively eliminating saturation effects and enhancing LCC and FVC estimation accuracy. (2) Ensemble models outperform single-machine learning models in estimating LCC and FVC, providing greater precision. Remarkably, the stacking + DF strategy achieved optimal performance in estimating LCC (coefficient of determination (R²): 0.930; root mean square error (RMSE): 3.974; average absolute error (MAE): 3.096); and FVC (R²: 0.716; RMSE: 0.057; and MAE: 0.044). (3) The proposed ANMD algorithm combined with LCC and FVC maps can be used to effectively monitor maize maturity. Establishing the maturity threshold for LCC based on the wax ripening period (P5) and successfully applying it to the wax ripening-mature period (P5–P7) achieved high monitoring accuracy (overall accuracy (OA): 0.9625–0.9875; user’s accuracy: 0.9583–0.9933; and producer’s accuracy: 0.9634–1). Similarly, utilizing the ANMD algorithm with FVC also attained elevated monitoring accuracy during P5–P7 (OA: 0.9125–0.9750; UA: 0.878–0.9778; and PA: 0.9362–0.9934). This study offers robust insights for future agricultural production and breeding, offering valuable insights for the further exploration of crop monitoring technologies and methodologies.