Food production environments in low- and middle-income countries (LMICs) are recognized as posing significant and increasing risks to antimicrobial resistance (AMR), one of the greatest threats to global public health and food security systems. In order to maximize and expedite action in mitigating AMR, the World Bank and AMR Global Leaders Group have recommended that AMR is integrated into wider sustainable development strategies. Thus, there is an urgent need for tools to support decision makers in unravelling the complex social and environmental factors driving AMR in LMIC food-producing environments and in demonstrating meaningful connectivity with other sustainable development issues. Here, we applied the Driver-Pressure-State-Impact-Response (DPSIR) conceptual framework to an aquaculture case study site in rural Bangladesh, through the analysis of distinct social, microbiological, and metagenomic data sets. We show how the DPSIR framework supports the integration of these diverse data sets, first to systematically characterize the complex network of societal drivers of AMR in these environments and second to delineate the connectivity between AMR and wider sustainable development issues. Our study illustrates the complexity and challenges of addressing AMR in rural aquaculture environments and supports efforts to implement global policy aimed at mitigating AMR in aquaculture and other rural LMIC food-producing environments.

In terms of hatchery-based seed production, one of the most important aquaculture species in Bangladesh is the stinging catfish (Heteropneustes fossilis). Scientific and evidence-based embryonic and larval development research on this fish species in the context of climate change is limited. This experimental study was conducted via induced breeding of stinging catfish using a conventional hatchery system, rearing the larvae in hapas placed in ponds. A series of microscopic observations using a trinocular digital microscope and an analysis of the relationship between larval growth and climate-driven water quality parameters such as temperature, pH, dissolved oxygen, total dissolved solids, alkalinity, and ammonia were performed. During embryonic development, the first cleavage was observed between 30 and 35 min of post-fertilization. Embryonic development (ranging from the 2-cell to the pre-hatching stage) took 21:00 h. Hatching occurred at 22:30 to 23:00 h after fertilization, with an average larvae length of 2.78 ± 0.04 mm. In the post-hatching stage, four pairs of tiny barbels appeared at 36:00 h, and the larvae started feeding exogenously after 72:00 h. These larvae fully absorbed their yolk sacs on the 6th day and attained an average length of 6.44 ± 0.06 mm. Aerial respiration of the larvae was investigated through naked-eye observation on the 10th day of hatching. The average length of the larvae was 32.00 ± 2.0 mm at the end of the 30-day post-hatching observation period. Bivariate correlation analysis showed significant correlations between key climatic variables and water quality parameters under hapa-based larval-rearing conditions. According to canonical correlation analysis, the first canonical function revealed the highest significant correlation between the two sets of variables (r1 = 0.791). The response variable weight of larvae (6.607) was linked to two explanatory variables: pH (0.321) and dissolved oxygen (0.265). For the second canonical correlation function, a positive correlation (0.431) was observed between the two sets of variables. Larval weight (−18.304) was observed to be linked to climatic variables, including air temperature (−0.316) and surface pressure (0.338). Results of this study reveal the subtle correlation between larval growth and water quality driven by climatic variables.

Though different types of commercial probiotics are supplemented in biofloc technology (BFT), very little information is available on their effects on the farmed fish. Therefore, this study focused on evaluating the effects of three most commonly used commercial probiotics on the growth performance, intestinal histomorphology, and intestinal microbiota of Nile tilapia (Oreochromis niloticus) reared in BFT. Tilapia fry, with an average weight of 3.02 ± 0.50 g, were stocked at a density of 60 fry/0.2 m³, and cultured for 90 days. Three commercial probiotics were administered, with three replications for each: a single-genus multi-species probiotic (Bacillus spp.) (T1), a multi-genus multi-species probiotic (Bacillus sp., Lactobacillus sp., Nitrosomonas sp., Nitrobacter sp.) (T2), and a multi-species probiotic (Bacillus spp.) combined with enzymes including amylase, protease, cellulase, and xylanase (T3). The results showed significant variations in growth and feed utilization, with T3 outperforming other treatments in terms of weight gain, liver weight, and intestine weight. Adding Bacillus spp. with enzymes (T3) to water significantly increased the histomorphological parameters (villi length, villi depth, crypt depth, muscle thickness, intestinal thickness) as well as microbes (total viable count and total lactic acid bacteria) of intestine of fish compared to T1 and T2, leading to improved digestion and absorption responses. It is concluded that the supplementation of commercial probiotics has potential benefits on farmed fish species in BFT.

This study examines the impacts of climate change on fisheries and aquaculture in Bangladesh, one of the most climate-vulnerable countries in the world. The fisheries and aquaculture sectors contribute significantly to the national GDP and support the livelihoods of 12% of the total population. Using a Critical Literature Review (CLR) approach, peer-reviewed articles, government reports, and official datasets published between 2006 and 2025 were reviewed across databases such as Scopus, Web of Science, FAO, and the Bangladesh Department of Fisheries (DoF). The analysis identifies major climate drivers, including rising temperature, erratic rainfall, salinity intrusion, sea-level rise, floods, droughts, cyclones, and extreme events, and reviews their differentiated impacts on key components of the sector: inland capture fisheries, marine fisheries, and aquaculture systems. For inland capture fisheries, the review highlights habitat degradation, biodiversity loss, and disrupted fish migration and breeding cycles. In aquaculture, particularly in coastal systems, this study reviews the challenges posed by disease outbreaks, water quality deterioration, and disruptions in seed supply, affecting species such as carp, tilapia, pangasius, and shrimp. Coastal aquaculture is also particularly vulnerable to cyclones, tidal surges, and saline water intrusion, with documented economic losses from events such as Cyclones Yaas, Bulbul, Amphan, and Remal. The study synthesizes key findings related to climate-resilient aquaculture practices, monitoring frameworks, ecosystem-based approaches, and community-based adaptation strategies. It underscores the need for targeted interventions, especially in coastal areas facing increasing salinity levels and frequent storms. This study calls for collective action through policy interventions, research and development, and the promotion of climate-smart technologies to enhance resilience and sustain fisheries and aquaculture in the context of a rapidly changing climate.

Embryonic and larval development of tilapia (Oreochromis niloticus) is very vulnerable to climate change. This study was conducted for an assessment of the embryonic and larval development of Nile tilapia in traditional hatchery and re-circulatory thermostatic systems. Daily changes in embryonic and larval development were measured through microscopic observation and image analysis in the laboratory. Climatic data and water quality parameters were measured every day using appropriate devices. Water temperature was varied with room temperature at the traditional hatchery system while it was maintained at 28.50 °C in the re-circulatory thermostatic system. A total of 200 unhatched eggs were stocked in every three trays of both systems. The egg diameters of the gastrula, segmentation, and pharyngula stages were measured at higher (2261.47 ± 81.66 µm, 2646.24 ± 17.98 µm, and 2710.90 ± 16.60 µm) in the re-circulatory thermostatic system than in the traditional hatchery system (2261.07 ± 81.52 µm, 2645.47 ± 18.24 µm, and 2710.01 ± 16.45 µm), respectively. For both systems, egg colors, egg size, black pigments, germinal ring, eye shape, tail, and heartbeat were determined through microscopic observation. Higher hatching and survival rates were found under the re-circulatory thermostatic system (95% and 97%) than under the traditional hatchery system (85% and 81%). About 6 h less hatching time was required under the re-circulatory thermostatic system than under the traditional system. At the end of 30 DAH (Days After Hatching), larval length and weight under the re-circulatory thermostatic system were found to be higher (15.736 ± 0.424 mm and 0.0528 ± 0.004 g) than under the traditional hatchery system (15.518 ± 0.415 mm and 0.050 ± 0.004 g), respectively. Larval growth patterns for both systems were found to have an exponential trend. PCA analysis revealed that two components were identified, one primarily associated with morphometric characteristics and the other with climatic and water quality parameters. These components showed that there were several interrelationships between the morphometric changes and the climatic and water quality parameters. The characteristic changes of larval development under the re-circulatory thermostatic system and the traditional hatchery system were found to be remarkably similar except for some deformities denoted under the traditional hatchery system. The changes of yolk sac, body pigmentation, dorsal and caudal fin shape, eye size, and head length and width were determined from 1 DAH to 30 DAH. After absorbing the yolk sac, ready-made feed was provided. The water temperature was varied from 30.50 °C to 35.50 °C in the traditional hatchery system. The highest air temperature and humidity were 33.87 °C and 69.94% while the lowest were 29.63 °C and 45.62%, respectively, in the traditional hatchery system. There has been no such comprehensive comparative study on hatchery production in Bangladesh, and therefore, further research might be carried out on broader aspects. This research would be highly beneficial for improving seed production at the tilapia fish hatchery level in the country.

An integrated multivariate approach was applied to gain a deeper understanding of the feeding biology of hilsa shad, Tenualosa ilisha, collected from six different aquatic habitats across Bangladesh. This approach involved linking climatic factors, ecological factors, plankton abundance in water, reproductive traits, and plankton ingestion data. Climatic data were obtained from the National Oceanic and Atmospheric Administration (NOAA) and Climate Data Online (CDO) databases on a monthly basis. Water quality parameters were observed on-site at various sampling sites. Plankton data from water bodies and hilsa guts were collected monthly from the study areas and analyzed in the laboratory. The results obtained were averaged for each month. The correlation tests, multivariate approaches, cluster analyses, and regression analyses revealed that the gonadosomatic index was primarily influenced by climatic factors, the abundance of ingested gut plankton, and heir compositions. The analysis of selectivity indices confirmed that plankton preferentially ingested selective taxa. Thirteen plankton groups were identified in the water column of six different hilsa habitats. The dominant phytoplankton groups were Bacillariophyceae (34–53%), Chlorophyceae (31–50%), Cyanophyceae (4–8%), and Euglenophyceae (1–3%). Additionally, Copepoda, Rotifera, and Cladocera were the most numerous zooplankton groups. Hilsa shad primarily consumed Bacillariophyceae (38–57%), Chlorophyceae (35–53%), and Cyanophyceae (4–6%). However, they also exhibited selective ingestion of higher quantities of Bacillariophyceae and Chlorophyceae to fulfill specific and unique metabolic needs. Cluster analysis revealed the variability of phytoplankton and zooplankton abundance in water and gut in relation to diverse locations. Combining all the datasets, a principal component analysis (PCA) was applied. The first two principal components explained more than 54% of the variability. The first component explained the association between the gonadosomatic index and eco-climatic factors, while the second component extracted the cluster of ingested gut plankton in relation to salinity and pH. Pearson’s correlations and linear regression analyses showed that the number of gut plankton had a positive influence on the gonadosomatic index (GSI). Finally, the outcomes from these extensive datasets have provided a better understanding of the selective feeding behavior and the influence of feeding biology on the gonadal maturation of T. ilisha. This understanding is likely to be useful for maintaining and improving the growth and productivity of the existing production systems for this transboundary species.