Fe₃O₄ nanoparticles were loaded into poly-l-lactide (PLLA) with concentrations of 2% and 5%, respectively, using an electrospinning method. In vivo animal experiments were then performed to evaluate the potential of the Fe₃O₄/PLLA nanofibrous material for bone tissue engineering applications. Bony defects with a diameter of 4 mm were prepared in rabbit tibias. Fe₃O₄/PLLA nanofibers were grafted into the drilled defects and histological examination and computed tomography (CT) image detection were performed after an eight-week healing period. The histological results showed that the artificial bony defects grafted with Fe₃O₄/PLLA nanofibers exhibited a visibly higher bone healing activity than those grafted with neat PLLA. In addition, the quantitative results from CT images revealed that the bony defects grafted with 2% and 5% Fe₃O₄/PLLA nanofibers, respectively, showed 1.9- and 2.3-fold increases in bone volume compared to the control blank sample. Overall, the results suggest that the Fe₃O₄/PLLA nanofibers fabricated in this study may serve as a useful biomaterial for future bone tissue engineering applications.

The aim of this study was to fabricate biodegradable poly-l-lactic acid (PLLA) bone screws containing iron oxide (Fe₃O₄) nanoparticles, which are radiopaque and 3D-printable. The PLLA composites were fabricated by loading 20%, 30%, and 40% Fe₃O₄ nanoparticles into the PLLA. The physical properties, including elastic modulus, thermal properties, and biocompatibility of the composites were tested. The 20% nano-Fe₃O₄/PLLA composite was used as the material for fabricating the 3D-printed bone screws. The mechanical performance of the nano-Fe₃O₄/PLLA bone screws was evaluated by anti-bending and anti-torque strength tests. The tissue response and radiopacity of the nano-Fe₃O₄/PLLA bone screws were assessed by histologic and CT imaging studies using an animal model. The addition of nano-Fe₃O₄ increased the crystallization of the PLLA composites. Furthermore, the 20% nano-Fe₃O₄/PLLA composite exhibited the highest thermal stability compared to the other Fe₃O₄ proportions. The 3D-printed bone screws using the 20% nano-Fe₃O₄/PLLA composite provided excellent local tissue response. In addition, the radiopacity of the 20% nano-Fe₃O₄/PLLA screw was significantly better compared with the neat PLLA screw.

Starch-based biodegradable foams with a high starch content are developed using industrial starch as the base material and supercritical CO₂ as blowing or foaming agents. The superior cushioning properties of these foams can lead to competitiveness in the market. Despite this, a weak melting strength property of starch is not sufficient to hold the foaming agents within it. Due to the rapid diffusion of foaming gas into the environment, it is difficult for starch to maintain pore structure in starch foams. Therefore, producing starch foam by using supercritical CO₂ foaming gas faces severe challenges. To overcome this, we have synthesized thermoplastic starch (TPS) by dispersing starch into water or glycerin. Consecutively, the TPS surface was modified by compatibilizer silane A (SA) to improve the dispersion with poly(butylene adipate-co-terephthalate) (PBAT) to become (TPS with SA)/PBAT composite foam. Furthermore, the foam-forming process was optimized by varying the ratios of TPS and PBAT under different forming temperatures of 85 °C to 105 °C, and two different pressures, 17 Mpa and 23 Mpa were studied in detail. The obtained results indicate that the SA surface modification on TPS can influence the great compatibility with PBAT blended foams (foam density: 0.16 g/cm³); whereas unmodified TPS and PBAT (foam density: 0.349 g/cm³) exhibit high foam density, rigid foam structure, and poor tensile properties. In addition, we have found that the 80% TPS/20% PBAT foam can be achieved with good flexible properties. Because of this flexibility, lightweight and environment-friendly nature, we have the opportunity to resolve the strong demands from the packing market.

Low-molecular-weight hyaluronic acid (LMWHA) was integrated with superparamagnetic Fe₃O₄ nanoparticles (Fe₃O₄ NPs). The size distribution, zeta potential, viscosity, thermogravimetric and paramagnetic properties of the LMWHA-Fe₃O₄ NPs were systematically examined. For cellular experiments, MCF7 breast cancer cell line was carried out. In addition, the cell targeting ability and characteristics of the LMWHA-Fe₃O₄ NPs for MCF7 breast cancer cells were analyzed using the thiocyanate method and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The experimental results showed that the LMWHA-Fe₃O₄ NPs were not only easily injectable due to their low viscosity, but also exhibited a significant superparamagnetic property. Furthermore, the in vitro assay results showed that the NPs had negligible cytotoxicity and exhibited a good cancer cell targeting ability. Overall, the results therefore suggest that the LMWHA-Fe₃O₄ NPs have considerable potential as an injectable agent for enhanced magnetic resonance imaging (MRI) and/or hyperthermia treatment in breast cancer therapy.

In this study, we report a novel approach to fabricate an organic/inorganic magnetic hybrid system capable of self-healing, wherein a polycaprolactone-poly(furfuryl glycidyl ether) copolymer (PCLF) serving as the structure template was first synthesized, followed by the incorporation of iron oxide nanoparticles-decorated multiwalled carbon nanotubes (IONPs-MWCNTs) and 1,1′-(methylenedi-4,1-phenylene)bismaleimide (BMI) into the polymer matrix to form a covalently crosslinked hybrid network via a Diels−Alder (DA) reaction. For this system, the reactive combination of diene and dienophile from furan/maleimide, MWCNT/furan, and MWCNT/maleimide could facilely induce multiple DA reactions that imparted a versatile route to efficiently introduce IONPs-MWCNTs into the organic polymer hosts, resulting in a uniform distribution of IONPs-MWCNTs that led to a hybrid system with superparamagnetic properties. Beside the magnetic behavior, such material synergistically exhibited a superior ability for healing scratch defects via a retro-DA reaction. Therefore, this crosslinked PCLF/BMI/IONPs-MWCNTs hybrid system which exhibits multifunctional properties including superparamagnetic behavior and self-repairability can serve as an intelligent material for developing advanced electromagnetic applications.