We present the design, fabrication, and characterisation of an array of optical slot-waveguide ring resonator sensors, integrated with microfluidic sample handling in a compact cartridge, for multiplexed real-time label-free biosensing. Multiplexing not only enables high throughput, but also provides reference channels for drift compensation and control experiments. Our use of alignment tolerant surface gratings to couple light into the optical chip enables quick replacement of cartridges in the read-out instrument. Furthermore, our novel use of a dual surface-energy adhesive film to bond a hard plastic shell directly to the PDMS microfluidic network allows for fast and leak-tight assembly of compact cartridges with tightly spaced fluidic interconnects. The high sensitivity of the slot-waveguide resonators, combined with on-chip referencing and physical modelling, yields a volume refractive index detection limit of 5 × 10⁻⁶ refractive index units (RIUs) and a surface mass density detection limit of 0.9 pg mm⁻², to our knowledge the best reported values for integrated planar ring resonators.

We demonstrate label-free molecule detection by using an integrated biosensor based on a ${\mathrm{Si}}_3{\mathrm{N}}_4∕{\mathrm{SiO}}_2$ slot-waveguide microring resonator. Bovine serum albumin (BSA) and anti-BSA molecular binding events on the sensor surface are monitored through the measurement of resonant wavelength shifts with varying biomolecule concentrations. The biosensor exhibited sensitivities of 1.8 and $3.2\mathrm{nm}∕(\mathrm{ng}∕{\mathrm{mm}}^2)$ for the detection of anti-BSA and BSA, respectively. The estimated detection limits are 28 and $16\mathrm{pg}∕{\mathrm{mm}}^2$ for anti-BSA and BSA, respectively, limited by wavelength resolution.

We report experimental results of label-free anti-bovine serum albumin (anti-BSA) antibody detection using a SOI planar photonic crystal waveguide previously bio-functionalized with complementary BSA antigen probes. Sharp fringes appearing in the slow-light regime near the edge of the guided band are used to perform the sensing. We have modeled the presence of these band edge fringes and demonstrated the possibility of using them for sensing purposes by performing refractive index variations detection, achieving a sensitivity of 174.8 nm/RIU. Then, label-free anti-BSA biosensing experiments have been carried out, estimating a surface mass density detection limit below 2.1 pg/mm² and a total mass detection limit below 0.2 fg.

We report an experimental demonstration of single-strand DNA (ssDNA) detection at room temperature using a photonic-crystal-waveguide-based optical sensor. The sensor surface was previously biofunctionalized with ssDNA probes to be used as specific target receptors. Our experiments showed that it is possible to detect these hybridization events using planar photonic-crystal structures, reaching an estimated detection limit as low as $19.8\mathrm{nM}$ for the detection of the complementary DNA strand.