Landraces and indigenous varieties comprise valuable sources of crop species diversity. Their utilization in plant breeding may lead to increased yield and enhanced quality traits, as well as resilience to various abiotic and biotic stresses. Recently, new approaches based on the rapid advancement of genomic technologies such as deciphering of pangenomes, multi-omics tools, marker-assisted selection (MAS), genome-wide association studies (GWAS), and CRISPR/Cas9 gene editing greatly facilitated the exploitation of landraces in modern plant breeding. In this paper, we present a comprehensive overview of the implementation of new genomic technologies and highlight their importance in pinpointing the genetic basis of desirable traits in landraces and indigenous varieties of annual, perennial herbaceous, and woody crop species cultivated in the Mediterranean region. The need for further employment of advanced -omic technologies to unravel the full potential of landraces and indigenous varieties underutilized genetic diversity is also indicated. Ultimately, the large amount of genomic data emerging from the investigation of landraces and indigenous varieties reveals their potential as a source of valuable genes and traits for breeding. The role of landraces and indigenous varieties in mitigating the ongoing risks posed by climate change in agriculture and food security is also highlighted.

Volatile Organic Compounds (VOCs) over the harvest period have been assessed in twenty-five strawberry genotypes cultivated in western Greece. Using liquid–liquid extraction and gas chromatography–mass spectrometry (GC–-MS), twenty-eight volatiles were monitored at early (T1) and mid-harvest (T3) time points to investigate the effect of the genotype and harvest time on strawberry volatilome. A quantitative impact of both harvest date and genotype on VOCs associated with aroma was demonstrated, with the most significant VOCs being terpenes, esters, and acids, followed by lactones and furanones. Harvest date was crucial for terpenoid and phenylpropanoid content, and important for esters, short-chain acids, and lactones. Six out of the twenty-five genotypes (four commercial varieties, including ‘Rociera’, ‘Victory’, ‘Leyre’, and ‘Inspire’, and two advanced selection genotypes (G2 and G8) were evaluated at two additional time points, covering the entire harvest season. The volatile levels were higher in fruits harvested at early stages (T1–T2) for most of the genotypes examined. The G2 genotype turned out to have a less ample but more stable volatile profile throughout harvesting, while ‘Victory’, ‘Leyre’, and ‘Inspire’ exhibited less abrupt changes than ‘Rociera’. This study demonstrates that the determination of VOCs provides significant information regarding the differences in strawberry genotypes related to aroma and enables the selection of genotypes based on specific VOCs content and/or volatile stability over the harvest period. Furthermore, this study pinpoints that growers could opt for optimal harvest dates based on the genotypes and the VOC content.

Soil salinity is a major constraint to strawberry cultivation, adversely affecting plant growth, yield, and fruit quality. Salinity stress triggers complex physiological and biochemical responses, including osmotic adjustment, antioxidant defense, ion homeostasis, and shifts in metabolite accumulation, ultimately reducing crop productivity and fruit quality. This review synthesizes current knowledge on strawberry responses to salinity, with emphasis on physiological mechanisms, genotypic variation in tolerance, and emerging biologically based approaches, such as biostimulants, small signaling molecules, and beneficial microorganisms, that alleviate salinity stress and enhance plant resilience. In parallel, recent advances in the identification of stress-responsive genes and regulatory pathways are discussed in the context of their relevance for breeding salt-resilient cultivars. This review also identifies critical gaps in current knowledge that, despite significant progress, hinder the translation of mechanistic insights into stable yield and fruit quality under field conditions. By integrating physiological knowledge with advances in biological and breeding-based approaches, together with long-term field validation, this review provides a comprehensive framework for improving strawberry performance under saline conditions and guiding future cultivation and genetic improvement strategies.

The increasing threat of salinity, exacerbated by climate change and unsustainable agricultural practices, necessitates innovative and sustainable crop management strategies to safeguard vegetable crop production and global food security. This review highlights a comprehensive framework that combines physiological insights with practical interventions aimed at enhancing salinity tolerance in vegetable crops. Key strategies include grafting, precision irrigation and fertilization, biofortification, and biostimulant application. These practices are applicable to both soil-based and soilless cultivation systems, offering broad relevance across diverse production environments. Combining and adapting these strategies to specific crops and environments is essential for developing sustainable, productive vegetable farming systems that can survive rising salinity and secure future food supplies. Future research focus on optimizing these integrated methods and elucidating their underlying mechanisms to enable wider and more effective adoption.

The conservation of endemic plant species has come into the global spotlight, not only because of their contribution to biodiversity but also their vulnerability and high extinction risk due to environmental and anthropogenic threats. Based on these developments, it is now essential to monitor and protect these species by applying integrated conservation strategies, especially in view of climate change, which is one of the most severe threats to plants. Genetic and genomic tools provide new potential in assessing and quantifying genetic diversity and thus can be utilized to devise conservation strategies and contribute to biodiversity conservation efforts. Greece comprises a plant biodiversity hotspot in the Mediterranean Basin with a wide variety of rare, threatened, and endemic plant taxa. In this review, we examine several cases where a broad spectrum of genetic tools has been utilized so far in the diversity assessment and conservation management of Greek Endemic Plants (GEPs). Following an extensive database search, we have identified and included in our final data collection 19 studies concerning 32 GEPs for which molecular markers have been used for the determination of population genetic structure and diversity assessment, while at the same time, the research outcomes have been taken into consideration for conservation management. The limited application of genetic and genomic tools in GEP management is demonstrated, while the significance of implementing a comprehensive conservation strategy that will integrate genetic analyses and the data derived therein is also highlighted.

This study presents a comparative transcriptomic analysis of three commercial strawberry cultivars: ‘Rociera’, ‘Calderon’, and ‘Victory’, aimed at uncovering the molecular basis of their distinct flavor and aroma profiles. Through RNA sequencing, we analyzed the transcriptomic landscape of these varieties, uncovering a notable array of differentially expressed genes (DEGs) between them. Specifically, ‘Rociera’ showed a higher count of DEGs compared to ‘Victory’, suggesting significant differences in gene expression related to flavor and aroma between those cultivars. Our further investigations revealed pivotal metabolic pathways— such as those involving furanones, amino acids, fatty acids, and terpenoids—participating in generating volatile organic compounds in strawberries. These pathways exhibited variety-specific expression patterns, underlining the genetic determinants of each variety’s unique sensory characteristics. Gene Ontology (GO) enrichment analysis underscored important pathways like phototropism, sugar-mediated signaling, and terpenoid biosynthesis, highlighting the genetic intricacy that influences strawberry flavor and aroma. Additionally, our study identified 31,677 single nucleotide polymorphisms (SNPs) with a significant impact on genes associated with volatile compound biosynthesis. Notably, SNPs linked to key enzymes such as lipoxygenase 6, omega-6 fatty acid desaturase (FaFAD1), and phenylalanine ammonia-lyase 1 (PAL) were discovered, shedding light on the genetic variations that underlie flavor differences. Therefore, this research advances our comprehension of the genetic elements that impact strawberry fruit flavor and aroma, offering invaluable insights for molecular breeding endeavors focused on enhancing strawberry flavor.