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Dr. Ausra Baradoke
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8Years (2019-Present)
(Calculated once Scilit publication data is synced: current year − year of first publication + 1)
Representative Publications
View All Publications12 February 2020 in Analytical Chemistry
Journal Article
ABAusra Baradoke
Ausra BaradokeRHRobert Hein
Robert HeinXLXiaoxiong Li
Xiaoxiong Li
JDJason J. Davis
Jason J. DavisJournal Article
Maryia DrobyshVLViktorija Liustrovaite
Viktorija LiustrovaiteABAusra Baradoke
Ausra BaradokeRVRoman Viter
Roman Viter
Chien-Fu Chen
Almira Ramanaviciene +1
In this research, we assessed the applicability of electrochemical sensing techniques for detecting specific antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike proteins in the blood serum of patient samples following coronavirus disease 2019 (COVID-19). Herein, screen-printed carbon electrodes (SPCE) with electrodeposited gold nanostructures (AuNS) were modified with L-Cysteine for further covalent immobilization of recombinant SARS-CoV-2 spike proteins (rSpike). The affinity interactions of the rSpike protein with specific antibodies against this protein (anti-rSpike) were assessed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. It was revealed that the SPCE electroactive surface area increased from 1.49 ± 0.02 cm2 to 1.82 ± 0.01 cm2 when AuNS were electrodeposited, and the value of the heterogeneous electron transfer rate constant (k0) changed from 6.30 × 10−5 to 14.56 × 10−5. The performance of the developed electrochemical immunosensor was evaluated by calculating the limit of detection and limit of quantification, giving values of 0.27 nM and 0.81 nM for CV and 0.14 nM and 0.42 nM for DPV. Furthermore, a specificity test was performed with a solution of antibodies against bovine serum albumin as the control aliquot, which was used to assess nonspecific binding, and this evaluation revealed that the developed rSpike-based sensor exhibits low nonspecific binding towards anti-rSpike antibodies.
1 May 2019 in Electrochimica Acta
Journal Article
ABAusra Baradoke
Ausra BaradokeBJBincy Jose
Bincy Jose
RPRasa Pauliukaite
Rasa Pauliukaite
RFRobert Forster
Robert ForsterJournal Article
Maryia DrobyshVLViktorija Liustrovaite
Viktorija LiustrovaiteABAusra Baradoke
Ausra BaradokeARAlma Rucinskiene
Alma RucinskieneAlmira Ramanaviciene
Arũnas Ramanavičius +10
The serologic diagnosis of coronavirus disease 2019 (COVID-19) and the evaluation of vaccination effectiveness are identified by the presence of antibodies specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this paper, we present the electrochemical-based biosensing technique for the detection of antibodies specific to the SARS-CoV-2 proteins. Recombinant SARS-CoV-2 spike proteins (rSpike) were immobilised on the surface of a gold electrode modified by a self-assembled monolayer (SAM). This modified electrode was used as a sensitive element for the detection of polyclonal mouse antibodies against the rSpike (anti-rSpike). Electrochemical impedance spectroscopy (EIS) was used to observe the formation of immunocomplexes while cyclic voltammetry (CV) was used for additional analysis of the surface modifications. It was revealed that the impedimetric method and the elaborate experimental conditions are appropriate for the further development of electrochemical biosensors for the serological diagnosis of COVID-19 and/or the confirmation of successful vaccination against SARS-CoV-2.
20 September 2023 in ACS Measurement Science Au
Journal Article
NZNemira Zilinskaite
Nemira Zilinskaite
Rajendra Prasad ShuklaABAusra Baradoke
Ausra BaradokeThis Review provides a comprehensive overview of 3D printing techniques to fabricate implantable microelectrodes for the electrochemical detection of biomarkers in the early diagnosis of cardiovascular and neurodegenerative diseases. Early diagnosis of these diseases is crucial to improving patient outcomes and reducing healthcare systems' burden. Biomarkers serve as measurable indicators of these diseases, and implantable microelectrodes offer a promising tool for their electrochemical detection. Here, we discuss various 3D printing techniques, including stereolithography (SLA), digital light processing (DLP), fused deposition modeling (FDM), selective laser sintering (SLS), and two-photon polymerization (2PP), highlighting their advantages and limitations in microelectrode fabrication. We also explore the materials used in constructing implantable microelectrodes, emphasizing their biocompatibility and biodegradation properties. The principles of electrochemical detection and the types of sensors utilized are examined, with a focus on their applications in detecting biomarkers for cardiovascular and neurodegenerative diseases. Finally, we address the current challenges and future perspectives in the field of 3D-printed implantable microelectrodes, emphasizing their potential for improving early diagnosis and personalized treatment strategies.
1 March 2019 in Electrochimica Acta
Journal Article
ABAusra Baradoke
Ausra BaradokeIPIsabel Pastoriza-Santos
Isabel Pastoriza-Santos
Elisa González-RomeroAbout Me
Principal Investigator Senior Researcher Dr. Ausra Baradoke
Dr. Ausra Baradoke - A researcher with 10+ years of work experience in 8 scientific groups (including University of Oxford). Her PhD was achieved in collaboration with the Center for Physical and Technological Sciences with Irish universities Dublin City University and Trinity College Dublin.
Dr. Ausra Baradoke received Innovate UK funding at University of Oxford, where she successfully published 2 high-impact publications and contributed to a patent in the field of electrochemical sensors. Among her scientific achievements, a total of 7 scientific publications in high-impact international scientific journals , patent application, and science popularized by presenting research at 27 conferences.
She carried out research experimental development (R&D) work in projects funded by the Education Exchange Support Fund, Erasmus +, Innovate UK, and Mobilitas Pluss. Also, she successfully implemented the "Intellect LT" project (VP2-1.3-uM-02-K-04-122) and contributed to METASENS (CH-3-SMM-01-11), G ‐ SUPERCAP (3200-P325, LAT-16002 ). The PI has work / cooperation experience in private companies in the field of sensor development at ERUMPO LTD and later at Osler Diagnostics LTD.
Dr. Ausra Baradoke has been supervising students of various levels (bachelor's, master's, doctoral) in universities: Vilnius University, Dublin City University, Trinity College Dublin, University of Tartu and University of Oxford.