Quantitative reconstructions of past land cover are necessary to determine the processes involved in climate–human–land-cover interactions. We present the first temporally continuous and most spatially extensive pollen-based land-cover reconstruction for Europe over the Holocene (last 11 700 cal yr BP). We describe how vegetation cover has been quantified from pollen records at a 1^∘ × 1^∘ spatial scale using the “Regional Estimates of VEgetation Abundance from Large Sites” (REVEALS) model. REVEALS calculates estimates of past regional vegetation cover in proportions or percentages. REVEALS has been applied to 1128 pollen records across Europe and part of the eastern Mediterranean–Black Sea–Caspian corridor (30–75^∘ N, 25^∘ W–50^∘ E) to reconstruct the percentage cover of 31 plant taxa assigned to 12 plant functional types (PFTs) and 3 land-cover types (LCTs). A new synthesis of relative pollen productivities (RPPs) for European plant taxa was performed for this reconstruction. It includes multiple RPP values (≥2 values) for 39 taxa and single values for 15 taxa (total of 54 taxa). To illustrate this, we present distribution maps for five taxa (Calluna vulgaris, Cerealia type (t)., Picea abies, deciduous Quercus t. and evergreen Quercus t.) and three land-cover types (open land, OL; evergreen trees, ETs; and summer-green trees, STs) for eight selected time windows. The reliability of the REVEALS reconstructions and issues related to the interpretation of the results in terms of landscape openness and human-induced vegetation change are discussed. This is followed by a review of the current use of this reconstruction and its future potential utility and development. REVEALS data quality are primarily determined by pollen count data (pollen count and sample, pollen identification, and chronology) and site type and number (lake or bog, large or small, one site vs. multiple sites) used for REVEALS analysis (for each grid cell). A large number of sites with high-quality pollen count data will produce more reliable land-cover estimates with lower standard errors compared to a low number of sites with lower-quality pollen count data. The REVEALS data presented here can be downloaded from https://doi.org/10.1594/PANGAEA.937075 (Fyfe et al., 2022).

A revised checklist of the fish fauna of the Black Sea is reported. This paper is the first attempt to present an actual Check List of the fishes in the Black Sea according to the data available in the Black Sea countries, as well as their current conservation status, enlisted in IUCN. The total number of the Black Sea fish species is 189. Concerning the conservation status only two species (1.06 %) are extinct (Acipenser nudiventris and A. sturio), 3.70 % are critically endangered, 16.40 % are vulnerable, 1.06% are endangered, for 10.58 % there is a lack of data, 26.46% has been classified in the category “Least concern”, 2.65 % are “Near threatened” and 38. 10% are “Not evaluated”.

Length-weight relationships (LWRs) of 16,842 specimens of fish caught in the Bulgarian Black Sea waters, covering 9 families, 10 genera, and 10 species, were studied. Spiny dogfish (Squalus acanthias L.), thornback ray (Raja clavata L.), sprat (Sprattus sprattus L.), pontic shad (Alosa immaculata Eichwald), anchovy (Engraulis encrasicolus L.), whiting (Merlangius merlangus euxinus Pallas), horse mackerel (Trachurus mediterraneus Aleev), bonito (Sarda sarda Bloch), round goby (Neogobius melanostomus Pallas), and turbot (Psetta maxima L.) were collected from May 2006 to December 2008. The fishes were caught by different fishing techniques, such as gill net, trawl net, and trap nets. The slope or allometric coefficient (b) of the functional regression between length and weight values varied from between 2.302 and 3.839 with the mean b = 3.152. The LWRs for S. acanthias and R. clavata have been reported for the first time for the Bulgarian Black Sea waters.

Wheat germ oil (WGO), derived from the nutrient-dense germ of wheat kernels, is a functional bioactive product, known for its rich composition of essential fatty acids, sterols, tocopherols, and polyphenols. This study aimed to comprehensively profile the molecular and therapeutic properties of WGO, focusing on its antioxidant, cytotoxic, and anti-inflammatory activity. Using advanced analytical techniques such as gas chromatography-mass spectrometry (GC-MS), Fourier Transform Infrared (FTIR) spectroscopy, and fluorescence analysis, WGO was shown to contain high levels of linoleic acid (45.3%), squalene (2.52 g/100 g), and polyphenols. WGO displayed selective cytotoxicity, inhibiting cancer cells’ viability in melanoma, prostate, and colorectal cancer cell lines, but not normal cells, highlighting its chemoprevention potential. Furthermore, WGO significantly reduced LPS-induced nitric oxide and IL-6 production in macrophages, with effects plateauing at higher doses. The 3D fluorescence spectra showed a significant decrease in fluorescence intensity when human serum albumin interacted with the WGO polyphenol fraction, indicating a strong binding affinity and stable complex formation. These findings emphasize the nutritional and therapeutic potential of WGO as a natural bioactive agent, warranting further mechanistic investigation and broader applications in health and disease management.